Nursing process: systematization of the nursing care instrument in the perception of nurses

Objective: this study aimed to identify the perception of nurses about the contribution of the nursing process for the systematization of nursing care performed in a Teaching Hospital in the South of Brazil. Methods: descriptive and exploratory qualitative study. The study participants were 38 nurses. The qualitative analysis was of the content and with the resources of the Atlas Ti® software. Results: after the analysis the following thematic categories were found: operational aspects of the nursing process; the nursing process and its practical application; facilities and difficulties in implementing the Nursing Process. Conclusion: the use of a care model to guide nursing practice for more than three decades has reflected positively on the quality of the assistance provided, as well as the satisfaction of nurses to perceive their work as recognized. The nursing process improves the systematization of nursing care, with the philosophy of the institution and of the Nursing Service aligned to a permanent education policy

Análise da influência de telas utilizadas como reforço em revestimento de argamassa de fachada frente a esforços de tração

Descolamentos e fissuras são manifestações patológicas recorrentes em revestimentos de fachadas de edificaçõesbrasileiras. Neste contexto, sabe-se que o revestimento de argamassa ainda é o mais utilizado no mercadoda construção civil e tem demonstrado diversos problemas decorrentes dos materiais e da execução demaneira inadequada. Portanto, o presente trabalho buscou avaliar a influência do uso de diferentes telas comoreforço de revestimento argamassado, com o objetivo de identificar qual apresenta o melhor desempenho.Foram utilizados três modelos de telas, dois metálicos galvanizados de diferentes malhas e espessuras e ummodelo de polietileno de alta densidade (PEAD). A fim de caracterizar a tela isoladamente quanto à resistênciaà tração, foi realizado o ensaio de tração simples em corpos de prova de 250x300 mm para cada modelode tela. Para avaliar seu comportamento em conjunto com a argamassa, foi realizado o ensaio de tração naflexão de 4 pontos em corpos de prova prismáticos de 200x500 mm, no qual foram inseridas amostras dastelas nas posições de 15, 25 e 30 mm da base para um corpo de prova com espessura de 50 mm. Os resultadosobtidos indicam que a tela metálica eletrosoldada galvanizada, de malha quadrada 25x25 mm e fio de1,24 mm, apresentou o melhor desempenho dentre os modelos testados.Palavras-chave: Manifestações Patológicas; Revestimento de Argamassa; Telas; Resistência à Tração

Estudo experimental da influência da adição de microfibras de polipropileno e da cura na dureza superficial de pisos de concreto

As falhas de dosagem e execução de pisos de concreto, constantemente levam ao surgimento de manifestaçõespatológicas. Sendo assim, é indispensável o conhecimento dos fatores que influenciam o comportamentodo concreto ou alteram suas características, bem como a utilização de mão de obra de qualidade. Em vistadisso, este estudo avaliou a influência da adição de microfibras de polipropileno nos teores de 0 g/m³, 600g/m³ e 900 g/m³ e de diferentes condições de cura (sem cura, cura úmida e cura úmida após três dias de exposiçãoao ambiente) nos concretos empregados em pisos. Para tanto, foram realizados ensaios de exsudação,resistência à compressão uniaxial e dureza superficial por esclerometria. Os resultados indicam que a utilizaçãodas microfibras influenciou de forma significativa a exsudação e a resistência à compressão, ainda que adureza superficial não tenha sido afetada diretamente por este material e nem pelo tipo de cura. De maneirageral, pode-se dizer que dentre os teores de microfibras de polipropileno avaliados, o teor de 600 g/m³ é omais indicado para adição no concreto, apresentando melhor desempenho nas propriedades analisadas. Épossível afirmar ainda que a cura, apesar de não ter causado influência significativa na superfície do concreto,é um fator de extrema importância para suas propriedades de massa.Palavras-chave: Pisos de Concreto; Manifestações Patológicas; Microfibras de Polipropileno; Cura; DurezaSuperficial

Degradación de hidrocarburos de petróleo por bacterias aisladas de manglares

The petroleum hydrocarbon contamination represents a worldwide problem, since its accumulation promotes a serious environmental impact. Thereby, the use of microorganisms, such as those from mangrove micro biota, as degrading agents of various carbon sources is poorly exploited in environmental remediation processes. Thus, this in vitro study evaluated the degrading potential of isolated bacteria from mangrove sediments in the degradation of petroleum hydrocarbons. Analysis of the genetic diversity using the 16S rRNA marker revealed closely related (99%) sequences with Proteobacterium, Pseudomonas and Exiguobacterium. Results showed the bacterial growth in the mineral saline medium (MSM) containing 1% petroleum or diesel, as carbon sources. This growth was determinated by optical density at 595 nm for 15 days, with sample withdrawal every 48 h. Bacterial growth indicated the hydrocarbon metabolization. However, bacteria were more efficient at degrading petroleum. Overall, experimental data displayed the potential application of these bacteria in bioremediation processes, due to their metabolic and adaptive capacities to grow in a rich hydrocarbon medium.Los hidrocarburos de petróleo representan un problema mundial, pues su acumulación promueve un serio impacto ambiental. Así, el uso de microorganismos, por ejemplo los de la microbiota de manglares, como agentes degradadores de diversas fuentes de carbono, es poco explotado en procesos de remediación ambiental. Así, este estudio evaluó in vitro el potencial degradador de bacterias aisladas de sedimento de manglar en la degradación de hidrocarburos. El análisis genético usando el marcador 16S rRNA reveló secuencias íntimamente relacionadas (99%) con Proteobacterium, Pseudomonas y Exiguobacterium. Los resultados mostraron el crecimiento de bacterias en medio salino mineral (MSM) conteniendo petróleo o diesel al 1%, como fuentes de carbono. Este crecimiento, determinado por densidad óptica (DO) a 595 nm durante 15 días, con toma de muestras a cada 48 h, indicó la matabolización de hidrocarburos. Sin embargo, las bacterias fueron más eficientes en degradarlos. Por lo tanto, los resultados muestran la potencial aplicación de las bacterias en procesos de biorremediación por su capacidad metabólica y adaptativa de crecimiento usando hidrocarburos

Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage

Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 µg/mL of EAE (11.29%) in comparison to 15.0 µg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 µg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. Conclusions: The extraction residue from neem leaf after hexane extraction is a source important of cytotoxic and genotoxic molecules against TK6 cells, the results also can suggest that the toxic effects in TK6 cells can be provided most likely due to the presence of high content of TPC from neem extracts.The CAPES (Coordination for the Improvement of Higher Education Personnel), Program Sandwich Abroad (Process: PDSE 99999.003409/15-5) provided funding for Klebson Silva Santos’ financial support and scholarship in Portugal. This study also was funded by CNPq (National Council for Scientific and Technological Development), and FAPITEC/SE (Fundação de Apoio à Pesquisa e à Inovação Tecnológica do Estado de Sergipe). The study also was carried out with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and the project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265. This work was also supported by the UIDB/50006/2020 project, funded by FCT/MCTES (Portugal, through national funds); SYSTEMIC “an integrated approach to the challenge of sustainable food systems: adaptive and mitigatory strategies to address climate change and malnutrition”, a Knowledge hub on Nutrition and Food Security, funded by FWO, INRA, BLE, MIPAAF, IZM, RCN, FCT and AEI in a joint action of JPI HDHL, JPI-OCEANS, and FACCE-JPI launched in 2019 under the ERA-NET ERA-HDHL [696295].info:eu-repo/semantics/publishedVersio

Corncob as carbon source in the production of xanthan gum in different strains Xanthomonas sp.

Xanthan gum (XG) is a biopolymer obtained in fermentation and used as a rheology control agent in aqueous systems and in stabilizing emulsions and suspensions. XG, together with other polysaccharides, can form soft, cohesive composite gels. The carbon source in the fermentative process is responsible for one-third of the production costs, and the search for less expensive and sustainable alternatives is ongoing. The use of agricultural residues such as the corncob is highly suggestive due to their abundance. This study aims to evaluate the use of derived hemicellulose fractions from the alkaline extraction of corncob as a carbon source in the production of XG in trials using four strains of Xanthomonas sp. (629, 1078, 254, and S6). The results indicate that strain 629 provides the higher yield (8.37 ± 5.75 g L−1) while using a fermentation medium containing a carbon source of saccharose (1.25%), hemicellulose fractions (3.75%), and salts. In this same medium, the strain 629 produces gum in 3% aqueous solution, showing the higher apparent viscosity (9298 ± 31 mPa s−1) at a shear rate of 10 s−1 at 25 °C. In conclusion, corncob is proven to be a promising sustainable alternative carbon source in the obtention of XG, improving the economic viability of the process within a biorefinery context. Saccharose must, however, also be included in the fermentation medium.0F1A-358B-43B5 | Fernando Jorge Ribeiro da MataN/

Essential oil of the leaves of Eugenia uniflora L.: Antioxidant and antimicrobial properties

AbstractEssential oil (EO) of the leaves of Eugenia uniflora L. (Brazilian cherry tree) was evaluated for its antioxidant, antibacterial and antifungal properties. The acute toxicity of the EO administered by oral route was also evaluated in mice. The EO exhibited antioxidant activity in the DPPH, ABTS and FRAP assays and reduced lipid peroxidation in the kidney of mice. The EO also showed antimicrobial activity against two important pathogenic bacteria, Staphylococcus aureus and Listeria monocytogenes, and against two fungi of the Candida species, C. lipolytica and C. guilliermondii. Acute administration of the EO by the oral route did not cause lethality or toxicological effects in mice. These findings suggest that the EO of the leaves of E. uniflora may have the potential for use in the pharmaceutical industry

Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction

Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25◦C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE)assolvents. Extractions using only ethanol(EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells.Klebson Silva Santos thanks CAPES (Process: PDSE 99999.003409/15-5) for the financial support during his Ph.D. studies in Portugal. Authors are grateful to CAPES, and FAPITEC (EDITAL CAPES/FAPITEC Nº 11/2016–PROEF/Processo de AUXPE 88881.157437/2017-01) for the financial support. The study was also carried out with financial support from FEDER, under the Partnership Agreement PT2020.info:eu-repo/semantics/publishedVersio