Isolation and identification of a male-produced aggregation-sex pheromone for the velvet longhorned beetle, Trichoferus campestris.

The velvet longhorned beetle, Trichoferus campestris (Faldermann) ("VLB"; Coleoptera: Cerambycidae), is native to eastern Asia where it infests and damages a wide range of deciduous and coniferous tree species, including orchard and timber species. Immature stages of VLB are transported to new countries via international commerce, and populations have established outside the native range of the species. Here, we show that identification of pheromones of invasive pest species can be expedited by knowledge of the semiochemistry of related taxa. Histological sectioning revealed subcuticular, male-specific prothoracic glands connected to pits in the cuticle, which, in related species, are diagnostic for production of male-produced aggregation-sex pheromones, usually characterized by 2,3-alkanediol/hydroxyketone structural motifs. However, in preliminary field bioassays, beetles were not attracted by any known cerambycid pheromones. Subsequently, we identified a novel variant of the hydroxyketone motif ("trichoferone") from headspace volatiles of males. In field bioassays, synthetic trichoferone was more attractive to both sexes of VLB than previously developed high-release-rate ethanol lures, and attraction was strongly female biased. This study demonstrated the utility of the prothoracic gland trait for predicting pheromone use in cerambycid species in the subfamily Cerambycinae, and that identification of pheromones of novel species can be expedited by knowledge of pheromones of related species. Trichoferone should prove to be a valuable tool for detection of VLB in regions where the beetle is or may become established

Detection and mapping of illicit drugs and their metabolites in fingermarks by MALDI MS and compatibility with forensic techniques

Despite the proven capabilities of Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS) in laboratory settings, research is still needed to integrate this technique into current forensic fingerprinting practice. Optimised protocols enabling the compatible application of MALDI to developed fingermarks will allow additional intelligence to be gathered around a suspect’s lifestyle and activities prior to the deposition of their fingermarks while committing a crime. The detection and mapping of illicit drugs and metabolites in latent fingermarks would provide intelligence that is beneficial for both police investigations and court cases. This study investigated MALDI MS detection and mapping capabilities for a large range of drugs of abuse and their metabolites in fingermarks; the detection and mapping of a mixture of these drugs in marks, with and without prior development with cyanoacrylate fuming or Vacuum Metal Deposition, was also examined. Our findings indicate the versatility of MALDI technology and its ability to retrieve chemical intelligence either by detecting the compounds investigated or by using their ion signals to reconstruct 2D maps of fingermark ridge details

Developing Legacy System Migration Methods and Tools for Technology Transfer

This paper presents the research results of an ongoing technology transfer project carried out in coopera- tion between the University of Salerno and a small software company. The project is aimed at developing and transferring migration technology to the industrial partner. The partner should be enabled to migrate monolithic multi-user COBOL legacy systems to a multi-tier Web-based architecture. The assessment of the legacy systems of the partner company revealed that these systems had a very low level of decompos- ability with spaghetti-like code and embedded control flow and database accesses within the user interface descriptions. For this reason, it was decided to adopt an incremental migration strategy based on the reengineering of the user interface using Web technology, on the transformation of interactive legacy programs into batch programs, and the wrapping of the legacy programs. A middleware framework links the new Web-based user interface with the Wrapped Legacy System. An Eclipse plug-in, named MELIS (migration environment for legacy information systems), was also developed to support the migration process. Both the migration strategy and the tool have been applied to two essential subsystems of the most business critical legacy system of the partner company

Identifying Cloned Navigational Patterns in Web Applications

Web Applications are subject to continuous and rapid evolution. Often programmers indiscriminately duplicate Web pages without considering systematic development and maintenance methods. This practice creates code clones that make Web Applications hard to maintain and reuse. We present an approach to identify duplicated functionalities in Web Applications through cloned navigational pattern analysis. Cloned patterns can be generalized in a reengineering process, thus to simplify the structure and future maintenance of the Web Applications. The proposed method first identifies pairs of cloned pages by analyzing similarity at structure, content, and scripting code. Two pages are considered clones if their similarity is greater than a given threshold. Cloned pages are then grouped into clusters and the links connecting pages of two clusters are grouped too. An interconnection metric has been defined on the links between two clusters to express the effort required to reengineer them as well as to select the patterns of interest. To further reduce the comprehension effort, we filter out links and nodes of the clustered navigational schema that do not contribute to the identification of cloned navigational patterns. A tool supporting the proposed approach has been developed and validated in a case study

Cooking influence on physico-chemical fruit characteristics of eggplant (Solanum melongena L.)

Physico-chemical traits of three eggplant genotypes ("Tunisina", "Buia" and "L 305") were evaluated before and after two cooking treatments (grilling and boiling). Different genotypes revealed different changes after cooking, with "Tunisina" showing a better retention of phytochemicals with respect to other two genotypes. The main physical phenomena were water loss during grilling, and dry matter loss after boiling. Chlorogenic acid, the main phenolic in eggplant, resulted higher in grilled samples, while delphinidin glycosides resulted more retained in boiled samples. Glycoalkaloids, thiols and biogenic amines were generally stable, while 5-hydroxy-methyl-furfural was found only in grilled samples. Interestingly, Folin-Ciocalteu index and free radical scavenging capacity, measured with three different assays, were generally increased after cooking, with a greater formation of antioxidant substances in grilled samples. NMR relaxation experiments clarified the hypothesis about the changes of eggplant compounds in terms of decomposition of larger molecules and production of small ones after cooking

Rocket salad: crop description, bioactive compounds and breeding perspectives

Rocket salad is a plant member of the Brassicaceae family whose name encloses species of the Eruca and Diplotaxis genera characterized by leaves with peculiar pungent taste and strong flavour. It has been originated in the Mediterranean area and nowadays is worldwide cultivated and consumed as food condiment and in ready-to-use mixed salad packages. Several other uses are recognized in cosmetics and medicine. This crop represents a valuable source of health benefits due to the presence of a range of health-promoting phytochemicals including carotenoids, vitamin C, fiber, polyphenols, and glucosinolates. These compounds are potentially linked in the prevention of certain diseases and types of cancer. Glucosinolates, represent the major class of compounds in rocket, and their hydrolysis products are responsible of the typical pungent aromas and flavours. Despite the continuous increase of the global consumption during the recent years, few efforts have been carried out in genetic improvement programs aimed to constitute new varieties due to biological and reproductive barriers. In the present article is provided a brief overview of the principal species of rocket salad used in dietary and discussed the qualitative properties as well as the potentiality and constraints for breeding

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Human rotaviruses represent a major cause of severe diarrheal disease in infants and young children. The limited impact of oral vaccines on global estimates of rotavirus mortality and the suboptimal use of oral rehydration justify the need for alternative prophylactic and therapeutic strategies, especially for immunocompromised hosts. The protective effects of colostrum\u2014the first milk produced during the initial 24 to 48 h after parturition\u2014are well documented in the literature. In particular, the ingestion of hyperimmune bovine colostrum has been proposed as an alternative preventive approach against human rotavirus gastroenteritis. Although the immunization of pregnant cows with human rotavirus boosts the release of specific immunoglobulin G in bovine colostrum, it raises regulatory and safety issues. In this study, we demonstrated that the conventional bovine rotavirus vaccine is sufficient to enhance the anti-human rotavirus protective efficacy of bovine colostrum, thus providing a conservative approach to produce hyperimmune bovine colostrum, making it exploitable as a functional food

Application of MALDI MS Imaging after sequential processing of latent fingermarks

Latent fingermarks are routinely visualised by subjecting them to one or more CSI/crime lab processes to maximise the recovery of ridge flow and minutiae permitting an identification. In the last decade mass spectrometric imaging (MSI) techniques have been applied to fingermarks to provide information about a suspect and/or on the circumstances of the crime as well as yielding additional images of the ridge pattern. In some cases, these techniques have shown the ability to provide further ridge detail, "filling in the gaps" of the developed mark. Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI MSI) is presently the most advanced of the so-called ‘surface analysis’ techniques, in terms of compatibility with a number of fingermark enhancement processes and implementation in operational casework. However, for the use of this technique in major crimes to become widespread, compatibility with sequential processing must be demonstrated. This short study has assessed compatibility with a number fingermark processing sequences applied to natural marks on the adhesive side of brown (parcel) and clear tapes. Within the study undertaken, the results confirm the possibility to use MALDI MSI in sequence with multiple processes offering in some instances, complementary ridge detail with respect to that recovered from marks developed by conventional sequence processing