A longitudinal examination of stressors, appraisals, and coping in youth swimming

Purpose Involvement in sport has the potential to cause athletes, coaches, and parents to experience stress. However, the extent to which experiences of stress are shared within the athletic triad is unknown. The purpose of this study was to examine the individual and shared stress experiences among youth swimmers, their mothers, and coach within the context of training, tapering, and competition. Design Multi-case study design. Method Four female swimmers, their mother, and one coach completed daily diaries for six weeks and up to three semi-structured interviews. Data were analyzed through within- and cross-case thematic analysis. Results The study showed evidence of shared stress experiences between all three members of the athletic triad. Participants predominately encountered organizational stressors, which they appraised in relation to movements between squad, interpersonal relationships, and overall progress towards performance goals/outcomes. Numerous coping strategies were employed by participants, with varying degrees of effectiveness, such as seeking social support, distancing, and lift sharing. The coping strategies used by coaches, swimmers, and parents were often interrelated with participants frequently seeking emotional support from one another. The majority of stressors and appraisals cited by parents and swimmers were shared, with both heavily relying on social support to help each other cope with the stressors encountered. Conclusion Athletes, parents, and coaches have the capacity to influence one another's stress experiences and as such their experiences should be considered simultaneously to maximize the impact of interventions

Emotional experiences in youth tennis

Abstract Objectives To explore adolescents' emotional experiences in competitive sport. Specifically, this study sought to identify, 1) The emotions adolescents' experience at tennis tournaments, 2) The precursors of the emotions they experience, and, 3) How adolescents attempt to cope with these emotions. Design Case-study. Method Four adolescent tennis players competed in four or five tennis matches under the observation of a researcher. Immediately following each match, participants completed a post-match review sheet and a semi-structured interview. A further semi-structured interview was completed at the end of the tournament. Review sheets, notes from match observations, and video recordings of matches were used to stimulate discussions during final interviews. All data were analyzed following the procedures outlined by Miles and Huberman (1994). Results Participants cited numerous positively and negatively valenced emotions during matches and tournaments. Participants' emotions seemed to be broadly influenced by their perceptions of performance and outcomes, as well as their opponent's behavior and player's perceptions of their own behavior. Participants described various strategies to cope with these emotions, such as controlling breathing rate, focusing on positive thoughts, and individualized routines. Further, if participants perceived them to be facilitative, negative emotions could be beneficial for performance. Conclusion This study provided original insights into the complexity of adolescent athletes' emotional experiences at competitions and highlights the critical need for further in-depth examinations of youth sport to fully comprehend the experiences of young people. Most notably, the findings highlight the necessity of considering the impact of both intra- and interpersonal influences on adolescents' emotional experiences, while also accounting for temporal changes

TAp73 promotes anabolism

Metabolic adaptation has emerged as a hallmark of cancer and a promising therapeutic target, as rapidly proliferating cancer cells adapt their metabolism increasing nutrient uptake and reorganizing metabolic fluxes to support biosynthesis. The transcription factor p73 belongs to the p53-family and regulates tumorigenesis via its two N-terminal isoforms, with (TAp73) or without (ΔNp73) a transactivation domain. TAp73 acts as tumor suppressor, at least partially through induction of cell cycle arrest and apoptosis and through regulation of genomic stability. Here, we sought to investigate whether TAp73 also affects metabolic profiling of cancer cells. Using high throughput metabolomics, we unveil a thorough and unexpected role for TAp73 in promoting Warburg effect and cellular metabolism. TAp73-expressing cells show increased rate of glycolysis, higher amino acid uptake and increased levels and biosynthesis of acetyl-CoA. Moreover, we report an extensive TAp73-mediated upregulation of several anabolic pathways including polyamine and synthesis of membrane phospholipids. TAp73 expression also increases cellular methyl-donor S-adenosylmethionine (SAM), possibly influencing methylation and epigenetics, and promotes arginine metabolism, suggestive of a role in extracellular matrix (ECM) modeling. In summary, our data indicate that TAp73 regulates multiple metabolic pathways that impinge on numerous cellular functions, but that, overall, converge to sustain cell growth and proliferation

Bitter Taste Receptor Agonists Mitigate Features of Allergic Asthma in Mice.

Asthma is characterized by airway inflammation, mucus secretion, remodeling and hyperresponsiveness (AHR). Recent research has established the bronchodilatory effect of bitter taste receptor (TAS2R) agonists in various models. Comprehensive pre-clinical studies aimed at establishing effectiveness of TAS2R agonists in disease models are lacking. Here we aimed to determine the effect of TAS2R agonists on features of asthma. Further, we elucidated a mechanism by which TAS2R agonists mitigate features of asthma. Asthma was induced in mice using intranasal house dust mite or aerosol ova-albumin challenge, and chloroquine or quinine were tested in both prophylactic and treatment models. Allergen challenge resulted in airway inflammation as evidenced by increased immune cells infiltration and release of cytokines and chemokines in the lungs, which were significantly attenuated in TAS2R agonists treated mice. TAS2R agonists attenuated features of airway remodeling including smooth muscle mass, extracellular matrix deposition and pro-fibrotic signaling, and also prevented mucus accumulation and development of AHR in mice. Mechanistic studies using human neutrophils demonstrated that inhibition of immune cell chemotaxis is a key mechanism by which TAS2R agonists blocked allergic airway inflammation and exerted anti-asthma effects. Our comprehensive studies establish the effectiveness of TAS2R agonists in mitigating multiple features of allergic asthma

Relative expression of TAp73 and ΔNp73 isoforms

The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other family members, transcribed as different isoforms with opposing pro- and anti-apoptotic functions. Unlike p53, p73 mutations are extremely rare in cancers. Instead, the pro-apoptotic activities of transcriptionally active p73 isoforms are commonly inhibited by over-expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoforms is critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N-terminal p73 isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform is abundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detected higher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues

Lexical coverage evaluation of large-scale multilingual semantic lexicons for twelve languages

The last two decades have seen the development of various semantic lexical resources such as WordNet (Miller, 1995) and the USAS semantic lexicon (Rayson et al., 2004), which have played an important role in the areas of natural language processing and corpus-based studies. Recently, increasing efforts have been devoted to extending the semantic frameworks of existing lexical knowledge resources to cover more languages, such as EuroWordNet and Global WordNet. In this paper, we report on the construction of large-scale multilingual semantic lexicons for twelve languages, which employ the unified Lancaster semantic taxonomy and provide a multilingual lexical knowledge base for the automatic UCREL semantic annotation system (USAS). Our work contributes towards the goal of constructing larger-scale and higher-quality multilingual semantic lexical resources and developing corpus annotation tools based on them. Lexical coverage is an important factor concerning the quality of the lexicons and the performance of the corpus annotation tools, and in this experiment we focus on evaluating the lexical coverage achieved by the multilingual lexicons and semantic annotation tools based on them. Our evaluation shows that some semantic lexicons such as those for Finnish and Italian have achieved lexical coverage of over 90% while others need further expansion

Release of Lungworm Larvae from Snails in the Environment: Potential for Alternative Transmission Pathways

Background: Gastropod-borne parasites may cause debilitating clinical conditions in animals and humans following the consumption of infected intermediate or paratenic hosts. However, the ingestion of fresh vegetables contaminated by snail mucus and/or water has also been proposed as a source of the infection for some zoonotic metastrongyloids (e.g., Angiostrongylus cantonensis). In the meantime, the feline lungworms Aelurostrongylus abstrusus and Troglostrongylus brevior are increasingly spreading among cat populations, along with their gastropod intermediate hosts. The aim of this study was to assess the potential of alternative transmission pathways for A. abstrusus and T. brevior L3 via the mucus of infected Helix aspersa snails and the water where gastropods died. In addition, the histological examination of snail specimens provided information on the larval localization and inflammatory reactions in the intermediate host. Methodology/Principal Findings: Twenty-four specimens of H. aspersa received ~500 L1 of A. abstrusus and T. brevior, and were assigned to six study groups. Snails were subjected to different mechanical and chemical stimuli throughout 20 days in order to elicit the production of mucus. At the end of the study, gastropods were submerged in tap water and the sediment was observed for lungworm larvae for three consecutive days. Finally, snails were artificially digested and recovered larvae were counted and morphologically and molecularly identified. The anatomical localization of A. abstrusus and T. brevior larvae within snail tissues was investigated by histology. L3 were detected in the snail mucus (i.e., 37 A. abstrusus and 19 T. brevior) and in the sediment of submerged specimens (172 A. abstrusus and 39 T. brevior). Following the artificial digestion of H. aspersa snails, a mean number of 127.8 A. abstrusus and 60.3 T. brevior larvae were recovered. The number of snail sections positive for A. abstrusus was higher than those for T. brevior. Conclusions: Results of this study indicate that A. abstrusus and T. brevior infective L3 are shed in the mucus of H. aspersa or in water where infected gastropods had died submerged. Both elimination pathways may represent alternative route(s) of environmental contamination and source of the infection for these nematodes under field conditions and may significantly affect the epidemiology of feline lungworms. Considering that snails may act as intermediate hosts for other metastrongyloid species, the environmental contamination by mucus-released larvae is discussed in a broader context

Overcoming the Impasse in Modern Economics

This document is the Accepted Manuscript version of the following article: Francesca Gagliardi, and David Gindis, 'Overcoming the Impasse in Modern Economics', Competition and Change, Vol. 15 (4): 336-42, November 2011, doi: 10.1179/102452911X13135903675732. Published by SAGE.Peer reviewe

Taxon-Specific Aerosolization of Bacteria and Viruses In an Experimental Ocean-Atmosphere Mesocosm

Ocean-derived, airborne microbes play important roles in Earth’s climate system and human health, yet little is known about factors controlling their transfer from the ocean to the atmosphere. Here, we study microbiomes of isolated sea spray aerosol (SSA) collected in a unique ocean–atmosphere facility and demonstrate taxon-specific aerosolization of bacteria and viruses. These trends are conserved within taxonomic orders and classes, and temporal variation in aerosolization is similarly shared by related taxa. We observe enhanced transfer into SSA of Actinobacteria, certain Gammaproteobacteria, and lipid-enveloped viruses; conversely, Flavobacteriia, some Alphaproteobacteria, and Caudovirales are generally under-represented in SSA. Viruses do not transfer to SSA as efficiently as bacteria. The enrichment of mycolic acid-coated Corynebacteriales and lipid-enveloped viruses (inferred from genomic comparisons) suggests that hydrophobic properties increase transport to the sea surface and SSA. Our results identify taxa relevant to atmospheric processes and a framework to further elucidate aerosolization mechanisms influencing microbial and viral transport pathways