Feeding behaviour of dairy cows in forced or free cow traffic in Automatic Milking System (AMS)

AbstractSeveral options have been suggested in the literature for "inviting" the lactating cows to a milking unit, minimizing the interference with the activities of the cows during the day (eating, drinking, resting) and optimizing the welfare of the animals. The aim of this research was to evaluate the effects of forced and free cow traffic on feeding behaviour in primiparous (PR) and pluriparous (PL) cows. The trial was carried out in the experimental free stall barn "V. Tadini" equipped with a single box automatic milking system (Galaxy, Milkline) and with a 40 lactating cows group. Cows were fed once a day (at 07:30 a.m.) the same Total Mixed Ration (TMR). During the trial two different cow traffic situations combined with different feeds management were tested. In the 1st period forced cow traffic (FC) was used; in the 2nd period free cow traffic (FR) but with an increase of the concentrate fed in the milking unit (+1.5 kg/head/day) and a reduction of the concentrate percentage in TMR (from 44.5 to ..

Host genetic basis of COVID-19: from methodologies to genes

The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is having a massive impact on public health, societies, and economies worldwide. Despite the ongoing vaccination program, treating COVID-19 remains a high priority; thus, a better understanding of the disease is urgently needed. Initially, susceptibility was associated with age, sex, and other prior existing comorbidities. However, as these conditions alone could not explain the highly variable clinical manifestations of SARS-CoV-2 infection, the attention was shifted toward the identification of the genetic basis of COVID-19. Thanks to international collaborations like The COVID-19 Host Genetics Initiative, it became possible the elucidation of numerous genetic markers that are not only likely to help in explaining the varied clinical outcomes of COVID-19 patients but can also guide the development of novel diagnostics and therapeutics. Within this framework, this review delineates GWAS and Burden test as traditional methodologies employed so far for the discovery of the human genetic basis of COVID-19, with particular attention to recently emerged predictive models such as the post-Mendelian model. A summary table with the main genome-wide significant genomic loci is provided. Besides, various common and rare variants identified in genes like TLR7, CFTR, ACE2, TMPRSS2, TLR3, and SELP are further described in detail to illustrate their association with disease severity

TRANSLATE -- A Monte Carlo Simulation of Electron Transport in Liquid Argon

The microphysics of electron and photon propagation in liquid argon is a key component of detector design and calibrations needed to construct and perform measurements within a wide range of particle physics experiments. As experiments grow in scale and complexity, and as the precision of their intended measurements increases, the development of tools to investigate important microphysics effects impacting such detectors becomes necessary. In this paper we present a new time-domain Monte Carlo simulation of electron transport in liquid argon. The simulation models the TRANSport in Liquid Argon of near-Thermal Electrons (TRANSLATE) with the aim of providing a multi-purpose software package for the study and optimization of detector environments, with a particular focus on ongoing and next generation liquid argon neutrino experiments utilizing the time projection chamber technology. TRANSLATE builds on previous work of Wojcik and Tachiya, amongst others, introducing additional cross-section processes up to ionization, thus modeling the full range of drift electron scattering interactions. The simulation is validated by benchmarking its performance with swarm parameters from data collected in experimental setups operating in gas and liquid.Comment: 17 pages, 12 figure

Ultra-High performance concrete (UHPC) with polypropylene (Pp) and steel Fibres: Investigation on the high temperature behaviour

Ultra-high performance concretes (UHPC) are advanced cement-based materials characterised by superior me-chanical properties with respect to normal and high-strength concretes; however, their dense and compact matrix can facilitate the onset of spalling at high temperatures. This problem is often coped up by adding polypropylene (PP) fibres to the mix design, alone or with other types of fibres; steel fibres enhance the material’s tensile ca-pacity. The paper presents a series of tests on two UHPC types (150 and 180 N/mm2) with PP fibres (0.27% of volume) and variable content of steel fibres (0% to 1.92%), aimed at investigating the residual mechanical properties of the material after high temperature exposure. The experimental results are compared to available research on small UHPC specimens exposed to high temperatures, with dosages in PP fibres from 0.03% to 2%, and in steel fibres from 0 to 3%. The results of this research demonstrate that UHPCs need hybrid fibre rein-forcement (PP +steel) to withstand high temperatures, and that the residual strength increases after 200 ◦C exposure, at all steel fibre dosages; this is in line with literature. Available research also shows that strength loss is possible in hot conditions, as found in the present research, while PP fibres alone do not always prevent the occurrence of spalling in small UHPC sample

Towards the Identification of a Suitable Commercial Diet for Carpione (Salmo carpio, Linnaeus 1758): A Multidisciplinary Study on Fish Performances, Animal Welfare and Quality Traits

SIMPLE SUMMARY: Carpione (Salmo carpio, Linnaeus 1758) is an endangered precious endemism of Lake Garda (Northern Italy), the largest Italian lake. To date, several bottlenecks about its culture remain unsolved, including the identification of a proper growth-out diet. In the present study, four different grossly isolipidic, isoproteic, and isoenergetic diets containing ingredients from different origins were used for S. carpio culture. Specifically, a diet largely based on marine ingredients, and currently used for carpione farming, was used as control. Three other diets were formulated in order to include relevant percentages of vegetable ingredients or processed animal proteins (at two different inclusion levels). After a three-month feeding trial, fish zootechnical performances, welfare, and flesh quality were evaluated through a multidisciplinary approach, including histology, gene expression, chemical analysis, and Fourier transform infrared spectroscopy (FTIR). This study provided the first insights on carpione physiological responses to different commercial dietary formulations. ABSTRACT: Carpione (Salmo carpio, Linnaeus 1758) is an endangered precious endemism of Lake Garda (Northern Italy), the largest Italian lake. To date, several bottlenecks about its culture remain unsolved, including the identification of a proper growth-out diet. The aim of the present study was to test four different grossly isolipidic, isoproteic, and isoenergetic diets in which the main ingredients had a different origin. Specifically, a diet currently used by local farmers for carpione culture, largely based on marine ingredients, was used as control (CTRL), while the other three diets were formulated by partially replacing marine ingredients with plant ones (VEG) or with different percentages of processed animal proteins (PAP1 and PAP2). The feeding trial was run in triplicate, over a three-month period. No significant differences in growth performance among the experimental groups were observed. However, remarkable histological alterations and inflammatory markers upregulation were observed in VEG group, while PAP inclusion played a role in attenuating inflammation and improving nutrient uptake. Fillet analyses highlighted significant differences in marketable traits and flesh fatty acid composition among the experimental groups, including the reduction of polyunsaturated fatty acids related to PAPs inclusion. In conclusion, PAPs used in the present study promoted S. carpio gut health and absorption capacity, while further studies are required to maintain proper quality traits of the final product

Measuring the effect of Mankai® (Wolffia globosa) on the gut microbiota and its metabolic output using an in vitro colon model

10openInternationalInternational coauthor/editorMankai® is a cultivated strain of Wolffia globosa an aquatic plant of the family Lemnaceae commonly known as Duckweeds. Recent studies suggest that consumption of a Mankai® enriched diet may provide positive health effects by decreasing body weight and improving glucose homeostasis and plasma lipid profiles. However, the effects of Mankai® alone on the composition and metabolic output of the human gut microbiota has not been fully investigated. Here, Mankai® was digested and fermented in vitro using a batch culture model of the proximal colon. Inulin and cellulose were used as readily and poorly fermentable control fibers respectively. Mankai® significantly stimulated the production of phenolic metabolites and short chain fatty acids by the gut microbiota (p<0.05). Three major microbial metabolites, 3-4-hydroxyphenyl propionic acid, 3-3-hydroxyphenyl propanoic acid and protocatechuic acid were significantly increased after 24 h fermentation. Moreover, Mankai® treatment lowered the overall microbial diversity (p<0.05), in line with a selective microbiome modulation.openDiotallevi, Camilla; Gaudioso, Giulia; Fava, Francesca; Angeli, Andrea; Lotti, Cesare; Vrhovsek, Urska; Rinott, Ehud; Shai, Iris; Gobbetti, Marco; Tuohy, KieranDiotallevi, C.; Gaudioso, G.; Fava, F.; Angeli, A.; Lotti, C.; Vrhovsek, U.; Rinott, E.; Shai, I.; Gobbetti, M.; Tuohy, K

CALR-positive myeloproliferative disorder in a patient with Ph-positive chronic myeloid leukemia in durable treatment-free remission: a case report

Current diagnostic criteria for Philadelphia-negative myeloproliferative neoplasia (MPN) have been redefined by the discovery of Janus kinase 2 (JAK2), myeloproliferative leukemia (MPL) and calreticulin (CALR) genetic alterations. Only few cases of coexistence of CALR-mutated MPN and Philadelphia-positive chronic myeloid leukemia (CML) have been described so far. Here we report the case of a patient with CML diagnosed in 2001, treated with imatinib and pegylated interferon (IFN) frontline. She reached complete molecular remission (CMR) and discontinued imatinib, maintaining treatment free remission. Due to persistent thrombocytosis, we repeated bone marrow (BM) analysis and diagnosed CARL-mutated essential thrombocythemia (ET). A CALR-positive clone was found to be present since 2001, and was unaffected by imatinib treatment, possibly representing a molecular abnormality arising at stem cell level

Treatment-Free Remission in Chronic Myeloid Leukemia Harboring Atypical BCR-ABL1 Transcripts

Discontinuation of tyrosine kinase inhibitors (TKI) is the main goal today in the field of Philadelphia positive chronic myeloid leukemia (Ph + CML) and the criteria to attempt the interruption of therapy are well defined and rely on the possibility to regularly monitor the BCR-ABL1 transcript. Patients harboring atypical transcripts are automatically excluded from protocols due to the absence of a standardized method of quantification of their minimal residual disease (MRD). We report here the outcome of 6 patients with atypical transcripts with a long follow up whose MRD was followed in three cases with digital PCR during their treatment free remission (TFR)