Far-Ultraviolet Dust Albedo Measurements in the Upper Scorpius Cloud Using the SPINR Sounding Rocket Experiment

The Spectrograph for Photometric Imaging with Numeric Reconstruction (SPINR) sounding rocket experiment was launched on 2000 August 4 to record far-ultraviolet (912-1450 A) spectral and spatial information for the giant reflection nebula in the Upper Scorpius region. The data were divided into three arbitrary bandpasses (912-1029 A, 1030-1200 A, and 1235-1450 A) for which stellar and nebular flux levels were derived. These flux measurements were used to constrain a radiative transfer model and to determine the dust albedo for the Upper Scorpius region. The resulting albedos were 0.28+/-0.07 for the 912-1029 A bandpass, 0.33+/-0.07 for the 1030-1200 A bandpass, and 0.77+/-0.13 for the 1235-1450 A bandpass

Plume mapping and isotopic characterisation of anthropogenic methane sources

Methane stable isotope analysis, coupled with mole fraction measurement, has been used to link isotopic signature to methane emissions from landfill sites, coal mines and gas leaks in the United Kingdom. A mobile Picarro G2301 CRDS (Cavity Ring-Down Spectroscopy) analyser was installed on a vehicle, together with an anemometer and GPS receiver, to measure atmospheric methane mole fractions and their relative location while driving at speeds up to 80 kph. In targeted areas, when the methane plume was intercepted, air samples were collected in Tedlar bags, for delta C-13-CH4 isotopic analysis by CF-GC-IRMS (Continuous Flow Gas Chromatography-Isotope Ratio Mass Spectrometry). This method provides high precision isotopic values, determining delta C-13-CH4 to +/- 0.05 per mil. The bulk signature of the methane plume into the atmosphere from the whole source area was obtained by Keeling plot analysis, and a delta C-13 -CH4 signature, with the relative uncertainty, allocated to each methane source investigated. Both landfill and natural gas emissions in SE England have tightly constrained isotopic signatures. The averaged delta C-13-CH4 for landfill sites is -58 +/- 3%o. The delta C-13-CH4 signature for gas leaks is also fairly constant around -36 +/- 2 parts per thousand, a value characteristic of homogenised North Sea supply. In contrast, signatures for coal mines in N. England and Wales fall in a range of -51.2 +/- 0.3 parts per thousand to 30.9 +/- 1.4 parts per thousand, but can be tightly constrained by region. The study demonstrates that CRDS-based mobile methane measurement coupled with off-line high precision isotopic analysis of plume samples is an efficient way of characterising methane sources. It shows that iiotopic measurements allow type identification, and possible location of previously unknown methane sources. In modelling studies this measurement provides an independent constraint to determine the contributions of different sources to the regional methane budget and in the verification of inventory source distribution. (C) 2015 Elsevier Ltd. All rights reserved

Tracer Measurements in Growing Sea Ice Support Convective Gravity Drainage Parameterizations

Gravity drainage is the dominant process redistributing solutes in growing sea ice. Modeling gravity drainage is therefore necessary to predict physical and biogeochemical variables in sea ice. We evaluate seven gravity drainage parameterizations, spanning the range of approaches in the literature, using tracer measurements in a sea ice growth experiment. Artificial sea ice is grown to around 17 cm thickness in a new experimental facility, the Roland von Glasow air‐sea‐ice chamber. We use NaCl (present in the water initially) and rhodamine (injected into the water after 10 cm of sea ice growth) as independent tracers of brine dynamics. We measure vertical profiles of bulk salinity in situ, as well as bulk salinity and rhodamine in discrete samples taken at the end of the experiment. Convective parameterizations that diagnose gravity drainage using Rayleigh numbers outperform a simpler convective parameterization and diffusive parameterizations when compared to observations. This study is the first to numerically model solutes decoupled from salinity using convective gravity drainage parameterizations. Our results show that (1) convective, Rayleigh number‐based parameterizations are our most accurate and precise tool for predicting sea ice bulk salinity; and (2) these parameterizations can be generalized to brine dynamics parameterizations, and hence can predict the dynamics of any solute in growing sea ic

Morphogenesis and differentiation in Rhodomicrobium

Differentiation and morphogenesis in the budding photosynthetic bacterium Rhodomicrobium vannielii (Rm5) was investigated using batch and continuous culture techniques. A previously undocumented 'simplified' vegetative cell cycle was identified. 'Simplified' Rm 5 cells (with a developmental cycle resembling that of the prosthecate Hyphomicrobium, were isolated from the late exponential growth phase of batch cultures, or from continuous cultures grown under high CO₂ tensions. Expression of this cell type appears to be under environmental control although the possibility that such cells may be mutants has not been overlooked. Previous studies have associated swarm cell production with mother cells comprising multicellular arrays, but the existence of 'simplified' cells suggests that microcolony formation is not a prerequisite for swarm cell synthesis. The characteristics of this unusual cell type and the morphology and physiology were compared with cells of Rhodomicrobium expressing the ’normal’ developmental cycle. Soluble protein extracts of ’simplified’ Rm 5 cells were compared with those of the other cell types by polyacrylamide gel electrophoresis. Since reproducibility of results was difficult to achieve under batch culture conditions, the possibility of using a chemostat to perform simple physiological studies and optimise growth conditions was investigated. Experiments were performed in order to try and elucidate whether or not 'simplified' Rm 5 cells were mutations, or a form of cellular expression in response to particular environmental conditions. Although growth of Rm 5 is accompanied by obligate differentiation, life cycle variations such as exospore production and swarm cell formation are almost certainly environmentally induced. Preliminary investigations were made as to the effect of different environmental conditions on cellular expression in Rm 5. Light intensity, pH and CO₂ tension were involved with swarm cell production and development, whereas the presence of trace elements was a probable requirement for successful exospore formation