USING CALLING ACTIVITY TO PREDICT CALLING ACTIVITY: A CASE STUDY WITH THE ENDANGERED HOUSTON TOAD (BUFO [ANAXYRUS] HOUSTONENSIS)

Understanding anuran calling activity patterns is important for maximizing efficiency and value of call survey data collection and analyses. Previous studies have primarily focused on identifying and quantifying abiotic variables that influence anuran calling activity, and investigating relationships between calling activity and population estimates. In this study we investigated the use of a predictor pond approach to guide call survey effort. In this approach, calling activity at a subset of breeding sites (e.g., ponds) is used as a predictor of calling activity at additional breeding sites, with the goal being to minimize sampling effort while simultaneously maximizing sampling efficiency. We explored the efficiency of this approach using call survey data collected on the endangered Houston Toad (Bufo [Anaxyrus] houstonensis) at 15 known breeding ponds over 9 survey years. We found that if calling activity at 3 predictor ponds was used to decide if additional call surveys would occur at the remaining 12 ponds, we would have hypothetically correctly assumed calling activity was not occurring at non-predictor ponds on 92.1% of survey nights, and we would have hypothetically detected 93.9% of the total number of detected individuals over the 9 survey years. We found the predictor pond approach performed well in our case study, and believe it could be a valuable tool for many anuran monitoring programs

Ecología y comportamiento reproductivo de Eleutherodactylus aureolineatus (Anura, Brachycephalidae) del dosel de la cuenca del Amazonas Superior, Ecuador

Exploration and investigation of the anuran fauna in the canopies of the Upper Amazon Basin has led to the recent discoveries of new species of the genus Eleutherodactylus utilizing the microhabitat within bromeliads. Detailed information on the ecology and natural history of these communities are scarce due, in part, to the difficulty of accessing their habitat. New sampling methods for rainforest canopies have allowed for the collection and observation of the herpetofauna utilizing this habitat. Sexual size dimorphism in Eleutherodactylus aureolineatus, confirms placement in the E. unistrigatus species group and E. lacrimosus assemblage. The described microhabitat, vocalization characteristics, reproductive behavior and egg deposition of E. aureolineatus provides significant ecological background on this poorly documented group of frogs and their important "wetlands in the sky". The data and observations herein contribute to the overall understanding of the characters and ecological factors which define the E. lacrimosus assemblage.La exploración y la investigación de la fauna de anuros en el estrato del dosel de la cuenca del Amazonas Superior ha llevado a descubrimientos recientes de nuevas especies del genero Eleutherodactylus que utilizan el interior de las bromelidas como micro-hábitat. La información detallada en la ecología y la historia natural de estas comunidades es escasa debido en parte a la dificultad de acceso de su hábitat. Nuevos métodos de muestreo para doseles de selvas pluviales han tomado en cuenta la colecta y la observación de la herpetofauna que utiliza este hábitat. El dimorfismo sexual de tamaño en Eleutherodactylus aureolineatus confirma su posición dentro del grupo de especies de E. unistrigatus y la colección de E. lacrimosus. La descripción del micro-hábitat, características de vocalización, comportamiento reproductivo y el sitio de deposición de huevo de E. aureolineatus provee antecedentes ecológicos importantes para este grupo de ranas pobremente documentadas y sus importantes "pantanos en el cielo". Los datos y las observaciones que aquí se presentan, contribuyen a la comprensión general de los caracteres y los factores ecológicos que definen la colección de E. lacrimosus

A new species of the Eleutherodactylus lacrimosus assemblage (Anura, Brachycephalidae) from the lowland rainforest canopy of Yasuni National Park, Amazonian Ecuador

A new species of Eleutherodactylus from the lowland rainforest canopy in northeastern Amazonian Ecuador is described. It is placed in the Eleutherodactylus lacrimosus assemblage of the greater unistrigatus group. It is most similar to the sympatric species Eleutherodactylus lacrimosus, but differs by the lack of a papilla at the tip of the snout, tubercles on upper eyelids, tubercles on dorsum, dorsal markings, and larger body size, and the presence of lateral fringes on the fingers and a tarsal fold. The new species inhabits tank bromeliads in the upper strata of the rainforest canopy at heights of 23.5-38.0 m. The effects of implementation of canopy surveys on biological diversity are briefly discussed

Redução do forrageio do iguana-negro-de-cauda-espinhosa, Ctenosaura similis (Sauria: Iguanidae), na presença de estímulos do predador

The presence of a predator may have direct and indirect effects on the behavior of the prey. Although altered behavior may help prey avoid predators, it also can have a potential impact on critical activities such as foraging. Predator-prey interactions are routinely studied in laboratory-based experiments owing to the perceived difficulties of conducting such experiments in natural settings. We conducted an experimental study under field conditions in Palo Verde National Park in northwestern Costa Rica to assess behavioral responses of Black Spiny-tailed Iguanas (Ctenosaura similis) to the presence of predators and predator cues. Free-roaming iguanas were offered mango in designated areas in the presence of a predator (Boa constrictor), a predator cue (B. constrictor feces), and a control (no predator or predator cue). Results indicate that iguanas reduced their foraging efforts in the presence of both a predator and its cue.A presença de um predador pode exercer efeitos diretos e indiretos sobre o comportamento da presa. Embora a alteração do comportamento possa ajudar a presa a evitar predadores, pode também ter um impacto potencial sobre atividades críticas, como o forrageio. As interações predador-presa são comumente estudadas em experimentos de laboratório devido às dificuldades em conduzir experimentos sob condições naturais. Conduzimos um estudo experimental em campo no Parque Nacional Palo Verde, no noroeste da Costa Rica, para avaliar as respostas dos iguanas-negros-de-cauda-espinhosa (Ctenosaura similis) à presença de predadores e de seus estímulos. Oferecemos manga a iguanas livres em áreas pré-estabelecidas na presença de um predador (Boa constrictor), de um estímulo do predador (fezes de B. constrictor) e em uma áreacontrole (sem predador ou estímulo). Os resultados indicam que os iguanas reduziram seus esforços de forrageio tanto na presença do predador como de seu estímulo

Encontro casual na região central do Texas fornece informações sobre a ecologia da estivação de Siren nettingi (Caudata: Sirenidae)

Siren spp. costumam ser vertebrados dominantes nas áreas úmidas que ocupam e são conhecidas por estivar quando essas áreas úmidas secam. Considerações práticas limitam as observações in situ de indivíduos em estivação. Em 12 de outubro de 2021, descobrimos por acaso um agregado em estivação de Siren nettingi no condado de Bastrop, Texas, Estados Unidos. Essas salamandras foram escavadas em solo compacto e rochoso adjacente a uma estrada de caliche, em profundidades que variavam entre ~0,2 e 1,5 m. A vegetação dominante nesse local incluía Ulmus crassifolia, Persicaria sp. e várias espécies de gramíneas. Recuperamos 140 indivíduos, dos quais sete foram resgatados e 133 foram capturados vivos. Medimos 115 deles quanto ao comprimento rostro-cloacal (SVL) e observamos que o agregado era dominado por jovens. Estimamos uma densidade de estivação de 2,33 indivíduos/m2 que é comparável às densidades estimadas para populações sem estivação. No entanto, como não houve monitoramento para esse estudo, provavelmente tenha ocorrido um evento de mortalidade em massa. Portanto, sugerimos que a construção de estradas no habitat preferido seja considerada uma ameaça às populações dessas salamandras.Siren spp. are often dominant vertebrates in the wetlands they occupy and are known to estivate when such wetlands dry up. Practical considerationslimit in-situ observations of estivating individuals. On 12 October 2021, we incidentally discovered an estivating aggregate of Siren nettingi in Bastrop County, Texas, USA. These salamanders were excavated from compact, rocky soil adjacent to a caliche road, at depths that ranged between ~0.2 to 1.5 m. The dominant vegetation at this site included Ulmus crassifolia, Persicaria sp., and various grass species. We recovered 140 individuals of which seven were salvaged and 133 were captured live. We measured 115 of these for snout–vent length (SVL) and observed the aggregate was predominated by juveniles. We estimated an estivation density of 2.33 sirens/m2 that is comparable to densities estimated for non-estivating populations. However, in-lieu of monitoring that was in place for this study, we expect a mass mortality event would have likely occurred. We therefore suggest that roadway construction in preferred habitat be considered as a threat to siren populations

A Place to Call Home: Amphibian Use of Created and Restored Wetlands

Loss and degradation of wetland habitats are major contributing factors to the global decline of amphibians. Creation and restoration of wetlands could be a valuable tool for increasing local amphibian species richness and abundance. We synthesized the peer-reviewed literature addressing amphibian use of created and restored wetlands, focusing on aquatic habitat, upland habitat, and wetland connectivity and configuration. Amphibian species richness or abundance at created and restored wetlands was either similar to or greater than reference wetlands in 89% of studies. Use of created and restored wetlands by individual species was driven by aquatic and terrestrial habitat preferences, as well as ability to disperse from source wetlands. We conclude that creating and restoring wetlands can be valuable tools for amphibian conservation. However, the ecological needs and preferences of target species must be considered to maximize the potential for successful colonization and long-term persistence

Mitochondrial sequence data reveal population structure within Pustulosa pustulosa

Unionid mussels are among the most imperiled group of organisms in North America, and Pustulosa pustulosa is a freshwater species with a relatively wide latitudinal distribution that extends from southern Ontario, Canada, to Texas, USA. Considerable morphological and geographic variation in the genus Pustulosa (formerly Cyclonaias) has led to uncertainty over species boundaries, and recent studies have suggested revisions to species-level classifications by synonymizing C. aurea, C. houstonensis, C. mortoni, and C. refulgens with C. pustulosa (currently P. pustulosa). Owing to its wide range and shallow phylogenetic differentiation, we analyzed individuals of P. pustulosa using mitochondrial DNA sequence data under a population genetics framework. We included 496 individuals, which were comprised of 166 samples collected during this study and 330 additional sequences retrieved from GenBank. Pairwise ΦST measures based on ND1 data suggested there may be up to five major geographic groups present within P. pustulosa. Genetic differentiation between regions within Texas was higher compared to populations from the Mississippi and Great Lakes populations, which may reflect differences in historical connectivity. Mitochondrial sequence data also revealed varying demographic histories for each major group suggesting each geographic region has also experienced differential population dynamics in the past. Future surveys should consider exploring variation within species after phylogeographic delimitation has been performed. In this study, we begin to address this need for freshwater mussels via the P. pustulosa system

Predation and the Maintenance of Color Polymorphism in a Habitat Specialist Squamate

Multiple studies have addressed the mechanisms maintaining polymorphism within a population. However, several examples exist where species inhabiting diverse habitats exhibit local population-specific polymorphism. Numerous explanations have been proposed for the maintenance of geographic variation in color patterns. For example, spatial variation in patterns of selection or limited gene flow can cause entire populations to become fixed for a single morph, resulting in separate populations of the same species exhibiting separate and distinct color morphs. The mottled rock rattlesnake (Crotalus lepidus lepidus) is a montane species that exhibits among-population color polymorphism that correlates with substrate color. Habitat substrate in the eastern part of its range is composed primarily of light colored limestone and snakes have light dorsal coloration, whereas in the western region the substrate is primarily dark and snakes exhibit dark dorsal coloration. We hypothesized that predation on high contrast color and blotched patterns maintain these distinct color morphs. To test this we performed a predation experiment in the wild by deploying model snakes at 12 sites evenly distributed within each of the two regions where the different morphs are found. We employed a 2×2 factorial design that included two color and two blotched treatments. Our results showed that models contrasting with substrate coloration suffered significantly more avian attacks relative to models mimicking substrates. Predation attempts on blotched models were similar in each substrate type. These results support the hypothesis that color pattern is maintained by selective predation

Response to Therapeutic Sleep Deprivation: A Naturalistic Study of Clinical and Genetic Factors and Post-treatment Depressive Symptom Trajectory

Research has shown that therapeutic sleep deprivation (SD) has rapid antidepressant effects in the majority of depressed patients. Investigation of factors preceding and accompanying these effects may facilitate the identification of the underlying biological mechanisms. This exploratory study aimed to examine clinical and genetic factors predicting response to SD and determine the impact of SD on illness course. Mood during SD was also assessed via visual analogue scale. Depressed inpatients (n = 78) and healthy controls (n = 15) underwent ~36 h of SD. Response to SD was defined as a score of ≤ 2 on the Clinical Global Impression Scale for Global Improvement. Depressive symptom trajectories were evaluated for up to a month using self/expert ratings. Impact of genetic burden was calculated using polygenic risk scores for major depressive disorder. In total, 72% of patients responded to SD. Responders and non-responders did not differ in baseline self/expert depression symptom ratings, but mood differed. Response was associated with lower age (p = 0.007) and later age at life-time disease onset (p = 0.003). Higher genetic burden of depression was observed in non-responders than healthy controls. Up to a month post SD, depressive symptoms decreased in both patients groups, but more in responders, in whom effects were sustained. The present findings suggest that re-examining SD with a greater focus on biological mechanisms will lead to better understanding of mechanisms of depression