Measurement of collagen synthesis by cells grown under different mechanical stimuli

INTRODUCTION: The use of scaffolds in tissue engineering is essential to provide cells with a matrix for cell proliferation and differentiation resulting in tissue regeneration. Normally this process involves seeding cells onto an artificial biodegradable scaffold providing mechanical support for cells until there is sufficient extracellular matrix deposition (ECM) to replace the artificial scaffold. Collagen is the bulk protein found in the ECM and measurement of its synthesis is the most direct, absolute indicator of ECM production

A New High-intensity, Low-momentum Muon Beam for the Generation of Low-energy Muons at PSI

At the Paul Scherrer Institute (PSI, Villigen, Switzerland) a new high-intensity muon beam line with momentum p < 40MeV/c is currently being commissioned. The beam line is especially designed to serve the needs of the low-energy, polarized positive muon source (LE-μ+) and LE-μ SR spectrometer at PSI. The beam line replaces the existing μ E4 muon decay channel. A large acceptance is accomplished by installing two solenoidal magnetic lenses close to the muon production target E that is hit by the 590-MeV PSI proton beam. The muons are then transported by standard large aperture quadrupoles and bending magnets to the experiment. Several slit systems and an electrostatic separator allow the control of beam shape, momentum spread, and to reduce the background due to beam positrons or electrons. Particle intensities of up to 3.5 × 108 μ+/s and 107 μ−/s are expected at 28MeV/c beam momentum and 1.8mA proton beam current. This will translate into a LE-μ+ rate of 7,000/s being available at the LE-μ SR spectrometer, thus achieving μ+ fluxes, that are comparable to standard μ SR facilitie

Fast high fidelity quantum non-demolition qubit readout via a non-perturbative cross-Kerr coupling

Qubit readout is an indispensable element of any quantum information processor. In this work, we experimentally demonstrate a non-perturbative cross-Kerr coupling between a transmon and a polariton mode which enables an improved quantum non-demolition (QND) readout for superconducting qubits. The new mechanism uses the same experimental techniques as the standard QND qubit readout in the dispersive approximation, but due to its non-perturbative nature, it maximizes the speed, the single-shot fidelity and the QND properties of the readout. In addition, it minimizes the effect of unwanted decay channels such as the Purcell effect. We observed a single-shot readout fidelity of 97.4% for short 50 ns pulses, and we quantified a QND-ness of 99% for long measurement pulses with repeated single-shot readouts

Qubit readout using in-situ bifurcation of a nonlinear dissipative polariton in the mesoscopic regime

We explore the nonlinear response to a strong drive of polaritonic meters for superconducting qubit state readout. The two polaritonic meters result from the strong hybridization between a bosonic mode of a 3D microwave cavity and an anharmonic ancilla mode of the superconducting circuit. Both polaritons inherit a self-Kerr nonlinearity $U$, and decay rate $\kappa$ from the ancilla and cavity, respectively. They are coupled to a transmon qubit via a non-perturbative cross-Kerr coupling resulting in a large cavity pull $2\chi > \kappa, ~U$. By applying magnitic flux, the ancilla mode frequency varies modifying the hybridization conditions and thus the properties of the readout polariton modes. Using this, the hybridisation is tuned in the mesoscopic regime of the non-linear dissipative polariton where the self-Kerr and decay rates of one polariton are similar $U\sim \kappa$ leading to bistability and bifurcation behavior at small photon number. This bistability and bifurcation behavior depends on the qubit state and we report qubit state readout in a latching-like manner thanks to the bifurcation of the upper polariton. Without any external quantum-limited amplifier, we obtain a single-shot fidelity of $98.6\%$ in a $500$ ns integration time

Brazing techniques for the fabrication of biocompatible carbon-based electronic devices

Prototype electronic devices have been critical to the discovery and demonstration of the unique properties of new materials, including composites based on carbon nanotubes (CNT) and graphene. However, these devices are not typically constructed with durability or biocompatibility in mind, relying on conductive polymeric adhesives, mechanical clamps or crimps, or solders for electrical connections. In this paper, two key metallization techniques are presented that employ commercially-available brazing alloys to fabricate electronic devices based on diamond and carbonaceous wires. Investigation of the carbon - alloy interfacial interactions was utilized to guide device fabrication. The interplay of both chemical ( adhesive ) and mechanical ( cohesive ) forces at the interface of different forms of carbon was exploited to fabricate either freestanding or substrate-fixed carbonaceous electronic devices. Elemental analysis in conjunction with scanning electron microscopy of the carbon - alloy interface revealed the chemical nature of the Ag alloy bond and the mechanical nature of the Au alloy bond. Electrical characterization revealed the non-rectifying nature of the carbon - Au alloy interconnects. Finally, electronic devices were fabricated, including a Au circuit structure embedded in a polycrystalline diamond substrate

Evaluation Of Mechanical and Biocompatibility Properties of Hydroxyapatite/Manganese Dioxide Nanocomposite Scaffolds for Bone Tissue Engineering Application

The aim of this research was to evaluate the mechanical properties, biocompatibility, and degradation behavior of scaffolds made of pure hydroxyapatite (HA) and HA‐modified by MnO2 for bone tissue engineering applications. HA and MnO2 were developed using sol‐gel and precipitation methods, respectively. The scaffolds properties were characterized using X‐ray diffraction (XRD), Fourier transform spectroscopy (FTIR), scanning electron microcopy (SEM), energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). The interaction of scaffold with cells was assessed using in vitro cell proliferation and alkaline phosphatase (ALP) assays. The obtained results indicate that the HA/ MnO2 scaffolds possess higher compressive strength, toughness, hardness, and density when compared to the pure HA scaffolds. After immersing the scaffold in the SBF solution, more deposited apatite appeared on the HA/MnO2, which results in the rougher surface on this scaffold compared to the pure HA scaffold. Finally, the in vitro biological analysis using human osteoblast cells reveals that scaffolds are biocompatible with adequate ALP activit

Dark Sector Studies with Neutrino Beams

An array of powerful neutrino-beam experiments will study the fundamentalproperties of neutrinos with unprecedented precision in the coming years. Alongwith their primary neutrino-physics motivations, there has been growingrecognition that these experiments can carry out a rich program of searches fornew, light, weakly-coupled particles that are part of a dark sector. In thiswhite paper, we review the diverse theoretical motivations for dark sectors andthe capabilities of neutrino beam experiments to probe a wide range of modelsand signatures. We also examine the potential obstacles that could limit theseprospects and identify concrete steps needed to realize an impactful darksector search program in this and coming decades.<br

Dark Sector Studies with Neutrino Beams

An array of powerful neutrino-beam experiments will study the fundamentalproperties of neutrinos with unprecedented precision in the coming years. Alongwith their primary neutrino-physics motivations, there has been growingrecognition that these experiments can carry out a rich program of searches fornew, light, weakly-coupled particles that are part of a dark sector. In thiswhite paper, we review the diverse theoretical motivations for dark sectors andthe capabilities of neutrino beam experiments to probe a wide range of modelsand signatures. We also examine the potential obstacles that could limit theseprospects and identify concrete steps needed to realize an impactful darksector search program in this and coming decades.<br

Electric field-modulated non-ohmic behavior of carbon nanotube fibers in polar liquids.

We report a previously unseen non-ohmic effect in which the resistivity of carbon nanotube fibers immersed in polar liquids is modulated by the applied electric field. This behavior depends on the surface energy, dielectric constant, and viscosity of the immersion media. Supported by synchrotron SAXS and impedance spectroscopy, we propose a model in which the gap distance, and thus the conductance, of capacitive interbundle junctions is controlled by the applied field.JT acknowledges generous financial support from: The Cambridge Commonwealth European and International Trust, CONACyT (Mexico), Dyson Ltd, and Pembroke College Cambridge. JJV acknowledges support from MINECO (Spain) and FP7-People-Marie Curie Action-CIG.This is the accepted manuscript. The final version is available from ACS at http://pubs.acs.org/doi/abs/10.1021/nn5030835

Non-degenerate parametric amplifiers based on dispersion engineered Josephson junction arrays

Determining the state of a qubit on a timescale much shorter than its relaxation time is an essential requirement for quantum information processing. With the aid of a new type of non-degenerate parametric amplifier, we demonstrate the continuous detection of quantum jumps of a transmon qubit with 90% fidelity in state discrimination. Entirely fabricated with standard two-step optical lithography techniques, this type of parametric amplifier consists of a dispersion engineered Josephson junction (JJ) array. By using long arrays, containing $10^3$ JJs, we can obtain amplification at multiple eigenmodes with frequencies below $10~\mathrm{GHz}$, which is the typical range for qubit readout. Moreover, by introducing a moderate flux tunability of each mode, employing superconducting quantum interference device (SQUID) junctions, a single amplifier device could potentially cover the entire frequency band between 1 and $10~\mathrm{GHz}$.Comment: P.W. and I.T. contributed equally. 9 pages, 5 figures and appendice