Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.Fil: Fontana, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

Ricardo Rojas y el factor estudiantil en la Fundación de la Universidad Nacional de Cuyo

Fil: Fontana de García, María B.

Local anaesthesia efficacy as postoperative analgesia for open shoulder instability surgery. a prospective randomised controlled study

Background and objectives: The aim of present study was to evaluate for the first time, the clinical effect of local anaesthetic infiltration as postoperative analgesia in open shoulder surgery for anterior-inferior instability. The comparison of the local infiltration and interscalenic brachial plexus block to a control group test the local anaesthetic efficacy in this surgery. Methods: 78 patients scheduled for open shoulder surgery were enrolled and randomly assigned to one of three groups: local infiltration anaesthesia (LIA), interscalenic brachial plexus block (IBPB) and control (C). All patients received standardized general anaesthesia and all injections were performed with the same dose and volume of anaesthetic. The number boluses delivered by a PCA pump applied at the end of surgery and the visual analogue score (VAS) at 0, 2, 4, 6, 12, 18 and 24 hours after intervention were recorded. A patient satisfaction score was also assessed. Results: Mean bolus consumption of the rescue analgesic, compared to C, was significantly less both in the LIA and IBPB groups (P<0.05). The IBPB group showed VAS scores that were significantly better than C group at all time points (P<0.05). The VAS scores for LIA group were clinically comparable to IBPB, and only at the 2 and 6 hours, postoperative time points there were no significant differences found in respect to the C group. IBPB and LIA showed comparable patient satisfaction scores. Conclusion: The local anaesthetic infiltration as postoperative analgesia appears to be a clinically valid alternative, statistically comparable to IBPB, with no clinical meaningful adverse effects

Suitability of ground-based SfM-MVS for monitoring glacial and periglacial processes

Photo-based surface reconstruction is rapidly emerging as an alternative survey technique to lidar (light detection and ranging) in many fields of geoscience fostered by the recent development of computer vision algorithms such as structure from motion (SfM) and dense image matching such as multi-view stereo (MVS). The objectives of this work are to test the suitability of the ground-based SfM-MVS approach for calculating the geodetic mass balance of a 2.1km2 glacier and for detecting the surface displacement of a neighbouring active rock glacier located in the eastern Italian Alps. The photos were acquired in 2013 and 2014 using a digital consumer-grade camera during single-day field surveys. Airborne laser scanning (ALS, otherwise known as airborne lidar) data were used as benchmarks to estimate the accuracy of the photogrammetric digital elevation models (DEMs) and the reliability of the method. The SfM-MVS approach enabled the reconstruction of high-quality DEMs, which provided estimates of glacial and periglacial processes similar to those achievable using ALS. In stable bedrock areas outside the glacier, the mean and the standard deviation of the elevation difference between the SfM-MVS DEM and the ALS DEM was-0.42 \ub1 1.72 and 0.03 \ub1 0.74 m in 2013 and 2014, respectively. The overall pattern of elevation loss and gain on the glacier were similar with both methods, ranging between-5.53 and + 3.48 m. In the rock glacier area, the elevation difference between the SfM-MVS DEM and the ALS DEM was 0.02 \ub1 0.17 m. The SfM-MVS was able to reproduce the patterns and the magnitudes of displacement of the rock glacier observed by the ALS, ranging between 0.00 and 0.48 m per year. The use of natural targets as ground control points, the occurrence of shadowed and low-contrast areas, and in particular the suboptimal camera network geometry imposed by the morphology of the study area were the main factors affecting the accuracy of photogrammetric DEMs negatively. Technical improvements such as using an aerial platform and/or placing artificial targets could significantly improve the results but run the risk of being more demanding in terms of costs and logistics

Physical properties of z~4 LBGs: differences between galaxies with and without Ly-alpha emission

We have analysed the physical properties of z~4 Lyman Break Galaxies observed in the GOODS-S survey, in order to investigate the possible differences between galaxies where the Ly-alpha is present in emission, and those where the line is absent or in absorption. The objects have been selected from their optical color and then spectroscopically confirmed by Vanzella et al. (2005). From the public spectra we assessed the nature of the Ly-alpha emission and divided the sample into galaxies with Ly-alpha in emission and objects without Ly-alpha line (i.e. either absent or in absorption). We have then used the complete photometry, from U band to mid infrared from the GOODS-MUSIC database, to study the observational properties of the galaxies, such as UV spectral slopes and optical to mid-infrared colors, and the possible differences between the two samples. Finally through standard spectral fitting tecniques we have determined the physical properties of the galaxies, such as total stellar mass, stellar ages and so on, and again we have studied the possible differences between the two samples. Our results indicate that LBG with Ly-alpha in emission are on average a much younger and less massive population than the LBGs without Ly-alpha emission. Both populations are forming stars very actively and are relatively dust free, although those with line emission seem to be even less dusty on average. We briefly discuss these results in the context of recent models for the evolution of Lyman break galaxies and Ly-alpha emitters.Comment: Accepted for publication in A&A. Uses aa.cls, 6 pages, 3 figure

Serum resistin is causally related to mortality risk in patients with type 2 diabetes: Preliminary evidences from genetic data

Resistin has been firmly associated with all-cause mortality. We investigated, whether, in patients with type 2 diabetes (T2D), this association is sustained by a cause-effect relationship. A genotype risk score (GRS), created by summing the number of resistin increasing alleles of two genome-wide association studies (GWAS)-derived single nucleotide polymorphisms (SNPs), serum resistin measurements and allcause death records were obtained in 1,479 (403 events/12,454 person-years), patients with T2D from three cohorts, Gargano Heart Study-prospective design (n = 350), Gargano Mortality Study (n = 698) and Foggia Mortality Study (n = 431), from Italy. GRS was strongly associated with serum resistin in a non-linear fashion (overall p = 3.5 ∗ 10-7) with effect size modest for GRS = 1 and 2 and much higher for GRS &gt;3, with respect to GRS = 0. A significant non-linear association was observed also between GRS and all-cause mortality (overall p = 3.3 ∗ 10-2), with a low effect size for GRS = 1 and 2, and nearly doubled for GRS ≥ 3, with respect to GRS = 0. Based on the above-reported associations, each genetic equivalent SD increase in log-resistin levels showed a causal hazard ratio of all-cause mortality equal to 2.17 (95%CI: 1.22-3.87), thus providing evidence for a causal role of resistin in shaping the risk of mortality in diabetic patients