A cloud-based Cyber-Physical System for environmental monitoring

Cyber-Physical Systems (CPSs) represent a new generation of digital systems, where cyber entities and physical devices cooperate towards a set of common goals. The research presented in this paper aims to contribute to the development of CPSs by proposing an open architecture applicable in environmental monitoring, consisting of three layers. The paper provides a detailed description of each one of the CPS's main components. The bottom layer, composed of wireless sensor nodes, allows the acquisition of data and their transfer to the upper layers. The top and middle layers, composed of a measurements database, a knowledge base, a multi-agent society and web services, assure the proper operation of the CPS based on decision rules and complex data analyses. The proposed architecture provides a high degree of flexibility and scalability

Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-Open, and Desensitized States

SummaryIonotropic glutamate receptors (iGluRs) mediate the majority of fast excitatory signaling in the nervous system. Despite the profound importance of iGluRs to neurotransmission, little is known about the structures and dynamics of intact receptors in distinct functional states. Here, we elucidate the structures of the intact GluA2 AMPA receptor in an apo resting/closed state, in an activated/pre-open state bound with partial agonists and a positive allosteric modulator, and in a desensitized/closed state in complex with fluorowilliardiine. To probe the conformational properties of these states, we carried out double electron-electron resonance experiments on cysteine mutants and cryoelectron microscopy studies. We show how agonist binding modulates the conformation of the ligand-binding domain “layer” of the intact receptors and how, upon desensitization, the receptor undergoes large conformational rearrangements of the amino-terminal and ligand-binding domains. We define mechanistic principles by which to understand antagonism, activation, and desensitization in AMPA iGluRs

Flavodiiron Proteins in Oxygenic Photosynthetic Organisms: Photoprotection of Photosystem II by Flv2 and Flv4 in Synechocystis sp. PCC 6803

BACKGROUND: Flavodiiron proteins (FDPs) comprise a group of modular enzymes that function in oxygen and nitric oxide detoxification in Bacteria and Archaea. The FDPs in cyanobacteria have an extra domain as compared to major prokaryotic enzymes. The physiological role of cyanobacteria FDPs is mostly unknown. Of the four putative flavodiiron proteins (Flv1-4) in the cyanobacterium Synechocystis sp. PCC 6803, a physiological function in Mehler reaction has been suggested for Flv1 and Flv3. PRINCIPAL FINDINGS: We demonstrate a novel and crucial function for Flv2 and Flv4 in photoprotection of photosystem II (PSII) in Synechocystis. It is shown that the expression of Flv2 and Flv4 is high under air level of CO(2) and negligible at elevated CO(2). Moreover, the rate of accumulation of flv2 and flv4 transcripts upon shift of cells from high to low CO(2) is strongly dependent on light intensity. Characterization of FDP inactivation mutants of Synechocystis revealed a specific decline in PSII centers and impaired translation of the D1 protein in Delta flv2 and Delta flv4 when grown at air level CO(2) whereas at high CO(2) the Flvs were dispensable. Delta flv2 and Delta flv4 were also more susceptible to high light induced inhibition of PSII than WT or Delta flv1 and Delta flv3. SIGNIFICANCE: Analysis of published sequences revealed the presence of cyanobacteria-like FDPs also in some oxygenic photosynthetic eukaryotes like green algae, mosses and lycophytes. Our data provide evidence that Flv2 and Flv4 have an important role in photoprotection of water-splitting PSII against oxidative stress when the cells are acclimated to air level CO(2). It is conceivable that the function of FDPs has changed during evolution from protection against oxygen in anaerobic microbes to protection against reactive oxygen species thus making the sustainable function of oxygen evolving PSII possible. Higher plants lack FDPs and distinctly different mechanisms have evolved for photoprotection of PSII

Fractional order based velocity control system for a nanorobot in non-Newtonian fluids

Customized patient drug delivery overcomes classic medicine setbacks such as side effects, improper drug absorption or slow action. Nanorobots can be successfully used for targeted patient-specific drug administration, but they must be reliable in the entire circulatory system environment. This paper analyzes the possibility of fractional order control applied to the nanomedicine field. The parameters of a fractional order proportional integral controller are determined with the purpose of controlling the velocity of the nanorobot in non-Newtonian fluids envisioning the blood flow in the circulatory system

An autotuning method for a fractional order PD controller for vibration suppression

Fractional order controllers are receiving an ever-increasing interest from the research community due to their advantages. However, most of the tuning procedures for fractional order controllers assume a fully known mathematical model of the process. In this paper, an autotuning method for the design of a fractional order PD controller is presented and applied to the vibration suppression in airplane wings. To validate the designed controller, an experimental unit consisting of a smart beam that simulates the behaviour of an airplane wing is used. The experimental results demonstrate the efficiency of the designed controller in suppressing unwanted vibrations

A cloud-based Cyber-Physical System for environmental monitoring

Cyber-Physical Systems (CPSs) represent a new generation of digital systems, where cyber entities and physical devices cooperate towards a set of common goals. The research presented in this paper aims to contribute to the development of CPSs by proposing an open architecture applicable in environmental monitoring, consisting of three layers. The paper provides a detailed description of each one of the CPS's main components. The bottom layer, composed of wireless sensor nodes, allows the acquisition of data and their transfer to the upper layers. The top and middle layers, composed of a measurements database, a knowledge base, a multi-agent society and web services, assure the proper operation of the CPS based on decision rules and complex data analyses. The proposed architecture provides a high degree of flexibility and scalability