6 research outputs found
Antinociceptive and anti-inflammatory activities of ethanolic extract of Alafia barteri
This study analyzes the antinociceptive and anti-inflammatory properties of ethanolic leaf extract of Alafia barteri Oliv., Apocynaceae, based on its medicinal use in the treatment of toothaches, inflammation and fevers. The antinociceptive effect was assessed in mice using acetic acid-induced writhing, tail clip, tail immersion and formalin assays. Anti-inflammatory activity was evaluated on carrageenan-induced paw oedema in rats, and xylene-induced ear oedema in mice. In acetic acid-induced writhing test, the extract at different doses (50, 100 and 200 mg/kg, p.o.) significantly (p < 0.05) and dose-dependently reduced pain by 35.04, 56.49 and 84.25%, respectively. The extract also significantly inhibited both the early and late phases of formalin-induced nociception in mice. In the tail immersion test, the extract caused a significant inhibition of pain (34.43% inhibition, after 90 min) at a dose of 200 mg/kg, while the effect of the extract in the tail clip test was only significant at the 100 mg/kg dose. A. barteri caused a significant inhibition of paw oedema development in the carrageenan and xylene-induced oedema tests. There was no mortality recorded following treatment with the extract (5 g/kg, p.o.). The results support the traditional use of A. barteri in the treatment of various diseases associated with pain and inflammation
Gastroprotective Effects of DAS-77 (a Phytomedicine) in Ulcer Models in Rats
Purpose: DAS-77 is a phytomedicine that contains the dried bark of
Mangifera indica and root of Carica papaya . This study investigated
the antiulcer effects of DAS-77 in rats. Methods: DAS-77 was
administered orally twice daily for five consecutive days at doses of
50 - 400 mg/kg. Ulcer was induced in rats with ethanol, indomethacin,
pylorus ligation (PL) and cold restraint stress (CRS). Ulcer scores
were recorded based on examination of excised stomachs. Estimations of
gastric content volume, pH and titratable acidity in the PL model and
determination of the levels of antioxidants and malondialdehyde (MDA)
in gastric tissues in the CRS model were also done. Results: In all
the models, DAS-77 produced significant dose-dependent reductions in
ulcer score. Peak effects were produced at the dose of 400 mg/kg with
ulcer inhibition values of 98.57, 76.23, 99.28 and 96.70 % compared to
100.00, 93.79, 98.92 and 96.79 % for misoprostol/cimetidine,
respectively, for the ethanol, indomethacin, PL and CRS models. In the
PL model, DAS-77 caused a significant increase in pH of gastric content
but a reduction in volume and titratable acidity. At doses of 50 and
100 mg/kg in the CRS model, DAS-77 significantly increased the level of
reduced glutathione (GSH) and diminished MDA. Conclusion: The results
obtained in this study suggest that DAS-77 possesses gastroprotective
activity possibly due to reduced gastric secretion and acidity, and
antioxidant activity