Letter from Allan M. Fleming

Letter concerning payments for notes

Letter from Allan M. Fleming

Letter concerning payments for notes

Letters between Allan M. Fleming and William Kerr

Letters concerning expenses for an official trip of the Utah Agricultural College

Letters between Ida M. Snowden and W. J. Kerr, as well as letters of recommendation from Thomas H. Smith, Allan M. Fleming, J. L. Kendall, and W. W. Maughan

Letters concerning Mrs. Ida M. Snowden

Sequence divergence of Mus spretus and Mus musculus across a skin cancer susceptibility locus

<p>Abstract</p> <p>Background</p> <p><it>Mus spretus </it>diverged from <it>Mus musculus </it>over one million years ago. These mice are genetically and phenotypically divergent. Despite the value of utilizing <it>M. musculus </it>and <it>M. spretus </it>for quantitative trait locus (QTL) mapping, relatively little genomic information on <it>M. spretus </it>exists, and most of the available sequence and polymorphic data is for one strain of <it>M. spretus</it>, Spret/Ei. In previous work, we mapped fifteen loci for skin cancer susceptibility using four different <it>M. spretus </it>by <it>M. musculus </it>F1 backcrosses. One locus, <it>skin tumor susceptibility 5 </it>(<it>Skts5</it>) on chromosome 12, shows strong linkage in one cross.</p> <p>Results</p> <p>To identify potential candidate genes for <it>Skts5</it>, we sequenced 65 named and unnamed genes and coding elements mapping to the peak linkage area in outbred <it>spretus</it>, Spret/EiJ, FVB/NJ, and NIH/Ola. We identified polymorphisms in 62 of 65 genes including 122 amino acid substitutions. To look for polymorphisms consistent with the linkage data, we sequenced exons with amino acid polymorphisms in two additional <it>M. spretus </it>strains and one additional <it>M. musculus </it>strain generating 40.1 kb of sequence data. Eight candidate variants were identified that fit with the linkage data. To determine the degree of variation across <it>M. spretus</it>, we conducted phylogenetic analyses. The relatedness of the <it>M. spretus </it>strains at this locus is consistent with the proximity of region of ascertainment of the ancestral mice.</p> <p>Conclusion</p> <p>Our analyses suggest that, if <it>Skts5 </it>on chromosome 12 is representative of other regions in the genome, then published genomic data for Spret/EiJ are likely to be of high utility for genomic studies in other <it>M. spretus </it>strains.</p

Modelling, optimisation and decision support using the grid

Modelling, Optimisation and Decision Support tools are vital in most areas of engineering. As part of the Distributed Aircraft Maintenance Environment (DAME) e-Science project, a virtual “work-bench” has been developed that can aid the engineer in solving engineering design problems. While the problem that motivated the development of these tools was taken from the aerospace industry, this sort of approach has broader application in areas such as automotive and marine engineering, as well as in the medical industry

A novel human iPSC model of COL4A1/A2 small vessel disease unveils a key pathogenic role of matrix metalloproteinases

Cerebral small vessel disease (SVD) affects the small vessels in the brain and is a leading cause of stroke and dementia. Emerging evidence supports a role of the extracellular matrix (ECM), at the interface between blood and brain, in the progression of SVD pathology, but this remains poorly characterized. To address ECM role in SVD, we developed a co-culture model of mural and endothelial cells using human induced pluripotent stem cells from patients with COL4A1/A2 SVD-related mutations. This model revealed that these mutations induce apoptosis, migration defects, ECM remodeling, and transcriptome changes in mural cells. Importantly, these mural cell defects exert a detrimental effect on endothelial cell tight junctions through paracrine actions. COL4A1/A2 models also express high levels of matrix metalloproteinases (MMPs), and inhibiting MMP activity partially rescues the ECM abnormalities and mural cell phenotypic changes. These data provide a basis for targeting MMP as a therapeutic opportunity in SVD.</p

Cluster Control in Oligouranyl Complexes of p-t-Butylcalix[8]arene

Formation of uranyl ion complexes of p-t-butylcalix[8]arene by reaction of the calixarene with [UO2(dmso)5]2+ in the presence of various bases leads to the crystallisation of solids with interestingly different stoichiometry, involving both di- and tri-uranate oligomers bound to the calixarene in anionic species. In all, the calixarene hexa-anion is present in a virtually identical conformation, suggesting that conformational preferences of the ligand must be a major factor controlling the form of the bound oxo-metal complex. Hydrogen bonding to the anions does not appear to be prominent even in the presence of protonated amines and this may explain the formation of some remarkable cation/solvent/simple anion clusters found within the lattices

Deprotonation of large calixarenes-cation binding and conformations

© 2016 CSIRO. Single crystal X-ray studies of p-t-butylcalix[10]arene·2dmso·7H2O (1) and [NMe4][p-t-butylcalix[9]arene-H]·2dmso·H2O (2), provide new data on these large macrocycles and their conformations, that of 2 being the first where an encapsulated [NMe4]+ cation is present, while 1 contains the neutral ligand. Both were obtained as crystalline products of the reactions of the calixarenes with tetramethylammonium hydroxide after prolonged standing. The structure of [NEt4][calix[4]arene-H], in which the cation approaches inclusion in the shallow cone of the anion, is also defined and compared with various other alkylammonium derivatives of calixarenes as well as that of p-t-butylcalix[9]arene