Expression of chloramphenicol acetyltransferase in Bacillus subtilis under the control of a phytoplasma promoter

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Molecular characterization of a phytoplasma causing phyllody in clover and other herbaceous hosts in northern Italy

Red clover (Trifolium pratense) and Ladino clover (Trifolium repens) plants showing phytoplasma-associated symptoms (yellowing/reddening, virescence and phyllody) have been recovered in Friuli-Venezia Giulia, Italy. Using AluI RFLP analysis of PCR amplified 16S rDNA we showed that the disease can be caused independently by two phylogenetically distinct phytoplasmas. One of them showed the very typical 16S rDNA RFLP pattern of the agent of Clover Phyllody in Canada (CCPh). The 16S rDNA of the other phytoplasma (Italian Clover Phyllody phytoplasma, ICPhp) has been PCR amplified, cloned and sequenced. The sequence revealed high similarity (>98%) with phytoplasmas belonging to the X disease cluster, which includes organisms not reported to cause phyllody on their hosts. The analysis by AluI RFLP of the PCR amplified pathogen 16S rDNA from other herbaceous plants (Crepis biennis, Taraxacum officinale, Leucanthemum vulgare) collected nearby with phytoplasma-associated symptoms showed similar patterns. Southern blot hybridization of their EcoRI digested total DNA revealed identical RFLP patterns, suggesting that the causative agent may be the same organism

Fatigue resistance of low pressure nitrided Cr-Mo low alloy steels

Fatigue resistance of steel parts can be improved by nitriding, the more the deeper is the layer affected by nitrogen diffusion, as stated by the Lessells-Firrao law. Low-pressure nitriding has been applied to wheel's hubs fabricated with a quenched and tempered Cr-Mo low alloy steel. N diffusion depths have been compared to similar results obtained with the same type of automotive parts subjected to classical gas nitriding. 12 h total low-pressure treatment time provided an almost 100% increase of hardened depth over 40 h gas nitrided parts. Both treatments allowed reaching an adequate fatigue life, whereas induction hardened parts did not. X-ray diffraction analysis provided phase constitution of top converted layers in both nitriding cases. A rationale based on the effect of early treatment stage top layer formation has been developed

Comparison of Ni-Cr and Co-based alloys for fuel injectors

Presentazione orale al convegno TMS 2011 - 140th Annual Meeting & Exhibition, svoltosi a San Diego, USA, dal 27/02/2011 al 03/03/201

Fatigue behavior of dual-phase and TWIP steels for lightweight automotive structures

Presentazione orale al convegno Euromat 2011, svoltosi a Montpellier, Francia, dal 12/09/2011 al 15/09/201

Microstructural, mechanical and fatigue properties of Cobalt alloys

Presentazione orale al convegno TMS 2010 - 139th Annual Meeting & Exhibition, svoltosi a Seattle (USA) dal 14/02/2010 al 18/02/201

Molecular characterization of Aureobasidium spp. strains isolated during the cold season. A preliminary efficacy evaluation as novel potential biocontrol agents against postharvest pathogens

Several Aureobasidium spp. strains isolated from wild environments during winter 2022 were characterized by sequence analysis of the internal transcribed spacer (ITS), the translation elongation factor EF-1α gene (EF1), and part of the elongase gene (ELO). The variability in the EF1 and ELO loci are higher than in the ITS. All strains but one (UC14), were identified as A. pullulans. To assess the effectiveness of the characterized strains as biocontrol agents (BCAs) of diseases occurring during postharvest storage, a selection of the strains was evaluated by in vitro and in vivo assays. On average, the reduction of Monilinia spp. colony growth was more marked for non-volatile metabolites than for volatile (VOCs). Strain UC14 provided the strongest mycelial growth reduction of Monilinia fructicola by VOCs (66%). According to the in vivo results, all strains were effective in controlling brown rot during cold storage and remarkably in restricting the growth of Monilinia polystroma. In particular, VB23 was the most effective in controlling brown rot incidence, by 80%, 60%, 100%, and severity, by 79.5%, 72.7% and 100%, for Monilinia laxa, M. fructicola, and M. polystroma, respectively

Thermal diffusivity measurements of metastable austenite during continuous cooling

The thermal diffusivity of the metastable undercooled austenite is relevant for the quantitative analysis of the carbon and low-alloy steel quench. The standard laser-flash method requires prior thermal equilibrium between the sample and the furnace, which may not be possible to achieve without allowing the metastable phase to transform. Nevertheless, depending upon the steel's hardenability, the thermal transient due to a laser pulse may be much shorter than a cooling transient sufficiently steep to prevent the transformation of the austenite. In one such case, flash measurements were performed during continuous sample cooling and the thermal diffusivity of the metastable austenite was determined by using an extension of the standard analytical model. The adopted analytical model and data reduction procedure are described and the limitations and uncertainties of this method are discussed, also with the aid of a non-linear numerical simulation. The measured thermal diffusivity of the under cooled low-alloy austenite decreases linearly from 5.4•10−6 m2 s−1 at 1133 K to 4.3•10−6 m2 s−1 at 755 K; this trend is in broad agreement with one previous set of measurements upon a low-alloy undercooled austenite and with a large number of previous standard measurements upon stable (high-alloy) austenitic stainless steels

Studies on the aetiology of kiwifruit decline: interaction between soil-borne pathogens and waterlogging

Aims: In 2012, Italian kiwifruit orchards were hit by a serious root disease of unknown aetiology (kiwifruit decline, KD) that still causes extensive damage to the sector. While waterlogging was soon observed to be associated with its outbreak, the putative role of soil microbiota remains unknown. This work investigates the role of these two factors in the onset of the disease. Methods: Historical rainfall data were analysed to identify changes that might explain KD outbreak and mimic the flooding conditions required to reproduce the disease in a controlled environment. A greenhouse experiment was thus designed, and vines were grown in either unsterilized (U) or sterilized (S) soil collected from KD-affected orchards, and subjected (F) or not (N) to artificial flooding. Treatments were compared in terms of mortality rate, growth, and tissue modifications. Results: KD symptoms were only displayed by FU-treated vines, with an incidence of 90%. Ultrastructural observations detected tyloses and fibrils in the xylem vessels of all plants, irrespective of the treatment. Phytopythium vexans and Phytopythium chamaehyphon, isolated from roots of FU plants, emerged as the associated microorganisms. Conclusions: We succeeded in reproducing KD under controlled conditions and confirmed its association with both waterlogging and soil-borne microorganism(s)

Room temperature plastic flow localization in a Mn-alloyed austenitic steel

A fully austenitic steel containing 0.5 wt.% C and 22 wt.% Mn was recently proposed for the fabrication of automotive body structures by room-temperature sheet forming, the goal being weight reduction and better crash performance owing to its much higher yield stress and elongation (as compared to presently employed ferritic and multiphase steels). Full-thickness tensile specimens, cut from as-produced sheets, were polished and tested at different strain rates, and the macroscopic surface relief eventually induced by the plastic deformation was recorded with a video camera. Between 0.3 and 0.4 true strain, successive macroscopic deformation bands (forming about 45° angle with the tensile axis and involving the full specimen width) travel along the specimen, a new one being nucleated as the previous reaches one of the specimen heads, whereas the gage displacement vs. stress curve shows a series of steps, each corresponding to the transit of a band through the gage length, and the cross-head displacement vs. stress curve shows isolated stress peaks, each immediately preceding the nucleation of a new band. Afterwards, and up to rupture, a series of stationary deformation bands appear, most being immediately adjacent to the preceding ones, with the stress vs. strain curve showing a series of serrations with large stress drops. As the strain rate is increased from 0.0004 to 0.4 s-1, the overall flow stress slightly decreases and the mentioned plastic localization phenomena become less evident