Is arthroscopic intervention-assisted fragment-specific fixation technique superior to volar plating in distal radius fractures involving the middle column?

Purpose: In this study, the clinical and radiological results of volar plating and arthroscopic intervention-assisted fragment-specific fixation techniques used in patients operated for AO Type B-C distal radius fracture in which the medial colon is affected were compared. Materials and Methods: X-ray and clinical records of 98 patients who underwent surgical treatment for radius distal end (DER) fracture between April 2011 and January 2017. The patient groups treated with arthroscopy-assisted fragment-oriented fixation (Group A) and plate screw fixation with a volar approach without arthroscopic intervention (Group B) were compared in terms of joint range of motion, grip strength, and clinical scores. Flexion and extension, ulnar deviation, radial deviation, pronation, and supination angles measured with a standard goniometer were recorded in the joint range of motion measurement. Grip strength was measured with a Jamar dynamometer for power measurement. DASH score was used in clinical evaluation. Results: When the operated wrists of both patient groups were compared, there was no significant difference between the DASH score, grip strength, palmar flexion, and pronation angles of motion. A statistically significant difference was observed between the mean dorsiflexion angles of 55 degrees in group A and 44 degrees in group B, mean radial deviation angles of 25 and 19 degrees, and the mean supination angles of 87 and 80 degrees, respectively. Conclusion: Although arthroscopic-assisted fragmentspecific fixation is technically a laborious procedure that requires a learning curve in patients with intra-articular distal radius fractures, it may be beneficial to provide better postoperative results, especially in young patients with high range of motion expectations

Comparison of Three Sequential Extraction Methods for the Determination of Iron, Manganese, and Thallium in Asphaltite Samples

Asphaltite, which is based on petroleum, contains many elements that exist in different forms. In this study, the BCR sequential extraction (BCRSE), Tessier sequential extraction (TSE) and Stover sequential extraction (SSE) procedures were applied for the determination of Fe, Mn and Tl in different forms (exchangeable, carbonates, bound to organic and sulfide, and residual content) in the structure of asphaltite. The statistical relations between the metal values of the three different methods were determined and recommendations are given for the enrichment of these economically valuable elements from asphaltite samples. The most abundant form of Fe (%), Mn (mg kg(-1)) and Tl (mg kg(-1)) follows the order: reducible (2.28), residual (75.85) and residual (81.99) with BCRSE method; carbonates (2.46), residual (79.21) and residual (79.75) with TSE method; carbonates (2.36), residual (67.34) and residual (46.93) with SSE method. The Fe, Mn and Tl speciation scheme gives quantitative knowledge of toxicity, bioavailability, mobility, leachability and bioaccumulation, etc., for asphaltite. A comparison of the three methods shows that the TSE method is shorter and more economical, while SSE gives more detailed information about the chemical structure of the substances, and the BCRSE has reducible (as oxides) fractions for the metals. This is the first time that a comparison of the three extraction methods was carried out on asphaltites and reported. The certified reference material (CRM) NIST-1633b Coal Fly Ash was used to verify the accuracy of the method, and whether the results are in good agreement with the certified values

Research on Drawing Attention to Overlooked Viruses in Plant Viruses Causing Yellowing-Type Symptoms in Cucurbitaceae

A single-stranded, positive-sense plant RNA virus called the Cucurbit chlorotic yellows virus (CCYV, Crinivirus, Closteroviridae) is comprised of RNA1 and RNA2. It is semipersistently transmitted by the whitefly Bemisia tabaci biotypes MEAM1 and MED. In 2004, CCYV was discovered on melon plants in Japan. Several other cucurbit species, as well as a range of non-species, were subsequently reported from countries such as Saudi Arabia, California, Israel, Taiwan, Sudan, Lebanon, Iran, Greece, Türkiye, Egypt, Spain, and China. Whitefly populations are frequently encountered in cucurbit cultivating areas. Virus plant diseases attributed to whiteflies are common in squash cultivation in the open field and greenhouses in Türkiye, especially in cucumbers and squash. Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Tomato leaf curl New Delhi virus (ToLCNDV), Squash vein-yellowing virus (SqVYV) are the leading virus diseases transmitted by Bemisia tabaci. Symptoms caused specifically by these viral diseases are of the yellowing type, although they differ as intervascular yellowing, yellowing of old or young leaves, and upward curling. Besides these viruses, CCYV, a problem in cucurbits, is carried by whitefly like others, causes yellowing type symptoms, and its first record in cucumber was reported in 2017 in Türkiye. However, this virus is ignored in the production of cucurbits, it is a problem in production areas. There are major viruses cause similar symptoms with the presence of the Cucumber chlorotic yellows virus is overlooked in the symptomatological observation. In this study, we tried to reveal the difference of CCYV, which is encountered in cucurbit growing areas in Antalya and whose presence was detected by RT-PCR, from other viruses and emphasized its importance as a virus that should be considered in classical breeding studies

HOW USEFUL IS ELASTOGRAPHY IN THE FOLLOW-UP OF ACHILLES TENDON REPAIR?

Introduction: In addition to conservative modalities in the treatment of Achilles tendon injuries, open, percutaneous and minimally invasive semi-open techniques, as well as biological open surgical repair methods are used as surgical options. Compression elastography is one of the methods used for the follow-up of treatment in Achilles tendon injuries. Methods: 23 patients were included in our study between July 2013 and June 2014, as long as they had at least 4 years of follow-up. In the final control, the intact side and the operated side were both examined and compared. The variables were the American Orthopedic Foot and Ankle Score (AOFAS) which is measured as a functional score considering plantar flexion and dorsiflexion; calf circumference; Achilles tendon anteroposterior (AP) diameter; and elastographic examination. Results: The strain ratio value and AP diameter of the patients was significantly higher on the operated side than on the non-operated side (p 0.05). No correlation was observed between strain ratio and AOFAS (p: 0,995). Conclusion: Elastography is not a useful technique to evaluate functional results on long-term tendon healing. Level of Evidence III; Retrospective comparative study. © 2022. Acta Ortopedica Brasileira. All Rights Reserved.Thanks to Prof. Dr. Ebru Yeşildağ for consultation of study plannin

MOLECULAR CHARACTERIZATION OF ZYMV RESISTANT AND SUSCEPTIBLE CUCUMBER LINES

Circuit-level simulation is a computationally intensive problem that has proven to be particularly difficult to parallelize. While device evaluation can be performed in parallel in conventional circuit simulators, the execution overhead is high. We show that, by partitioning a circuit, OpenMP can be used to solve sub-circuits in different threads, without compromising accuracy. It is shown that execution time can be reduced proportionally to the number of threads

Mid-long Term Results of Dorsal Approach and Temporary Fixation Procedure in Overlooked Perilunate Carpal Dislocation

Aim:Our aim in this study is to present our mid-long term functional and radiological results after open reduction with dorsal approach, K-wire application and scapholunate ligament repair in cases with perilunate dislocation.Materials and Methods:Eleven patients who applied for perilunate dislocation between 2014 and 2018 and were followed up for at least 18 months were included in our study. The scapholunate angle, scapholunate gap, arthrosis, and avascular necrosis were evaluated in cases with isolated perilunate dislocations. The wrist flexion-extension and pronation-supination range of motion (ROM) of the patients were measured using a goniometer, and the wrist grip strength using a Jamar dynamometer. Functionally, the cases were evaluated according to the Disabilities of the Arm, Shoulder and Hand (DASH) scoring and the Herzberg clinical scoring system.Results:The mean age was 41.1 (28-64) years, the mean follow-up time was 3.8 (1.5-6) years. The mean scapholunate angle was 49.1±8.7 (37.3-70.4) degrees, the mean scapholunate gap was 2.0±0.5 (1.2-2.8) mm, the mean flexion-extension ROM was 131±17.5 (90-155) degrees, and the mean pronation-supination ROM was 155.4±6.1 (140-160) degrees. Patients had a mean DASH score of 5.6±3.2 (0-10.9) and a mean Herzberg clinical score of 89.1±9.4 (70-100).Conclusion:In perilunate dislocations, early and appropriate treatment is the main determinant of prognosis. Close follow-up of these injuries for at least 18 months is extremely important in terms of possible complications

Comparison of chemical and biological properties of in vivo and in vitro samples of Salvia siirtica Kahraman, Celep & Dogan extracts prepared with different solvents

© 2021The use of Salvia species among the public and their importance in the scientific world increase due to their numerous pharmacological and biological activities on a daily basis. In this study, the phytochemical contents of different parts (root, branch, leaf, flower, whole) of different specimens of Salvia siirtica (SS) (in vivo and in vitro) extracts prepared with various solvents (petroleum ether, chloroform and ethanol) were determined using different techniques (GC-MS and LC-MS/MS) and the results were compared. In addition, biological activities (antioxidant, cytotoxic, anticholinesterase antiurease, antityrosinase, antielastase and anticollagenase) of all samples were determined and compared. The antioxidant potential of the analysed samples was found to be high, and their enzyme activity potential was low. Besides, in vitro SS-TIS (Temporary Immersion System) sample showed high cytotoxic activity (viability% 2.12 ± 0.06) against MCF-7 (breast cancer) cell line. The results of GC-MS and LC-MS/MS analyses indicated that ferruginol and sugiol could be isolated from the ethanol extract of S. siirtica roots, salvigenin and β-sitosterol from the chloroform extract of the aerial parts, and phenolic compounds from the ethanol extract of the aerial parts. In addition, the amount of rosmarinic acid, caffeic acid and 12-demethylmulticauline of in vitro samples were found to be higher than those of in vivo samples. Furthermore, all samples, both in vivo and in vitro, contained high levels of rosmarinic acid and β-sitosterol. The whole chloroform extract (SSWC) could be the source extract for salvigenin (33952.13 µg/g) and β-sitosterol (16369.71 µg/g), and the root ethanol extract (SSRE) for ferruginol (17721.99 µg/g). As a result, it is understood that the nature of the plant material, the choice of an appropriate solvent and the parts of the material used are quite effective in chemical content. S. siirtica promise potential natural antioxidant agent in food and/or pharmaceutical industry due to its phytochemical content (rosmarinic and caffeic acids, ferruginol, salvigenin) and antioxidant activity

Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling

High mortality rates of glioblastoma (GBM) patients are partly attributed to the invasive behavior of tumor cells that exhibit extensive infiltration into adjacent brain tissue, leading to rapid, inevitable, and therapy-resistant recurrence. In this study, we analyzed transcriptome of motile (dispersive) and non-motile (core) GBM cells using an in vitro spheroid dispersal model and identified SERPINE1 as a modulator of GBM cell dispersal. Genetic or pharmacological inhibition of SERPINE1 reduced spheroid dispersal and cell adhesion by regulating cell-substrate adhesion. We examined TGFβ as a potential upstream regulator of SERPINE1 expression. We also assessed the significance of SERPINE1 in GBM growth and invasion using TCGA glioma datasets and a patient-derived orthotopic GBM model. SERPINE1 expression was associated with poor prognosis and mesenchymal GBM in patients. SERPINE1 knock-down in primary GBM cells suppressed tumor growth and invasiveness in the brain. Together, our results indicate that SERPINE1 is a key player in GBM dispersal and provide insights for future anti-invasive therapy design