Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway

Bioactive peptides are relevant nutritional factors that exhibit many functions including antioxidant, anti-hypertensive, anticancer and antimicrobial properties. In this paper, four synthetic peptides ARHPHPHLSFM (A-11-M), AVPYPQR (A-7-R), NPYVPR (N-6-R) and KVLPVPEK (K-8-K) with sequences present in milk proteinswere examined for their antioxidant properties. The compounds show moderate free radical scavenging activityin the ABTS and crocin assays (A-7-R and N-6-R) and lipid peroxidation inhibition in Caco-2 cells (N-6-R and K-8-K). All peptides, in particular K-8-K, activate the Keap1-Nrf2 system by allowing the translocation of the tran-scription factor Nrf2 from the cytosol to nucleus. This activation triggers the overexpression of the antioxidantenzymes Trx1, TrxR1, GR, NQO1 and SOD1. Furthermore, molecular modeling shows that K-8-K is able to hinderthe interaction of Nrf2 with Keap1. The reported results show that the antioxidant action in cells of thesebioactive peptides is mostly due to the activation of Keap1-Nrf2 signaling pathwa

Fundamental parameters of Cepheids. V. Additional photometry and radial velocity for southern Cepheids

I present photometric and radial velocity data for Galactic Cepheids, most of them being in the southern hemisphere. There are 1250 Geneva 7-color photometric measurements for 62 Cepheids, the average uncertainty per measurement is better than 0.01 mag. A total of 832 velocity measurements have been obtained with the CORAVEL radial velocity spectrograph for 46 Cepheids. The average accuracy of the radial velocity data is 0.38 km/s. There are 33 stars with both photometry and radial velocity data. I discuss the possible binarity or period change that these new data reveal. I also present reddenings for all Cepheids with photometry. The data are available electronically.Comment: To appear in ApJS. Data available electronically at ftp://cfa-ftp.harvard.edu/pub/dbersier

Assessment techniques, database design and software facilities for thermodynamics and diffusion

The purpose of this article is to give a set of recommendations to producers of assessed thermodynamic data, who may be involved in either the critical evaluation of limited chemical systems or the creation and dissemination of larger thermodynamic databases. Also, it is hoped that reviewers and editors of scientific publications in this field will find some of the information useful. Good practice in the assessment process is essential, particularly as datasets from many different sources may be combined together into a single database. With this in mind, we highlight some problems that can arise during the assessment process and we propose a quality assurance procedure. It is worth mentioning at this point, that the provision of reliable assessed thermodynamic data relies heavily on the availability of high quality experimental information. The different software packages for thermodynamics and diffusion are described here only briefly

Levantamento da ocorrência de populações do nematóide das galhas do cafeeiro (Meloidogyne sp.) em Rondônia.

Este trabalho teve como objetivo determinar a ocorrência e as principais espécies Meloidogyne que venham a ocorrer em lavouras de café rondonienses por meio da caracterização das espécies, a partir de análises morfológicas e isoenzimáticas de fêmeas do nematóide, coletadas de raízes e solo.bitstream/item/50269/1/cot332-nematoidedasgalhasdocafeeiro.pd

Ab initio simulations of the kinetic properties of the hydrogen monomer on graphene

The understanding of the kinetic properties of hydrogen (isotopes) adatoms on graphene is important in many fields. The kinetic properties of hydrogen-isotope (H, D and T) monomers were simulated using a composite method consisting of density functional theory, density functional perturbation theory and harmonic transition state theory. The kinetic changes of the magnetic property and the aromatic $\pi$ bond of the hydrogenated graphene during the desorption and diffusion of the hydrogen monomer was discussed. The vibrational zero-point energy corrections in the activation energies were found to be significant, ranging from 0.072 to 0.205 eV. The results obtained from quantum-mechanically modified harmonic transition state theory were compared with the ones obtained from classical-limit harmonic transition state theory over a wide temperature range. The phonon spectra of hydrogenated graphene were used to closely explain the (reversed) isotope effects in the prefactor, activation energy and jump frequency of the hydrogen monomer. The kinetic properties of the hydrogen-isotope monomers were simulated under conditions of annealing for 10 minutes and of heating at a constant rate (1.0 K/s). The isotope effect was observed; that is, a hydrogen monomer of lower mass is desorbed and diffuses more easily (with lower activation energies). The results presented herein are very similar to other reported experimental observations. This study of the kinetic properties of the hydrogen monomer and many other involved implicit mechanisms provides a better understanding of the interaction between hydrogen and graphene.Comment: Accepted by J. Phys. Chem.

New detectors for the kaon and hypernuclear experiments with KaoS at MAMI and with PANDA at GSI

The KaoS spectrometer at the Mainz Microtron MAMI, Germany, is perceived as the ideal candidate for a dedicated spectrometer in kaon and hypernuclei electroproduction. KaoS will be equipped with new read-out electronics, a completely new focal plane detector package consisting of scintillating fibres, and a new trigger system. First prototypes of the fibre detectors and the associated new front-end electronics are shown in this contribution. The Mainz hypernuclei research program will complement the hypernuclear experiments at the planned FAIR facility at GSI, Germany. At the proposed antiproton storage ring the spectroscopy of double Lambda hypernuclei is one of the four main topics which will be addressed by the PANDA Collaboration. The experiments require the operation of high purity germanium (HPGe) detectors in high magnetic fields (B= 1T) in the presence of a large hadronic background. The performance of high resolution Ge detectors in such an environment has been investigated.Comment: Presentation at International Symposium on the Development of Detectors for Particle, Astroparticle and Synchrotron Radiation Experiments, Stanford, Ca (SNIC06), 6 pages, LaTeX, 11 eps figure

Fermented soy-derived bioactive peptides selected by a molecular docking approach show antioxidant properties involving the keap1/nrf2 pathway

Bioactive peptides are a group of molecules with health beneficial properties, deriving from food matrices. They are protein fragments consisting of 2–20 amino acids that can be released by microbial fermentation, food processing and gastrointestinal digestion. Once hydrolyzed from their native proteins, they can have different functions including antioxidant activity, which is important for cell protection by oxidant agents. In this work, fermented soy products were digested in vitro in order to improve the release of bioactive peptides. These were extracted, purified and analyzed in vitro and in a cellular model to assess their antioxidant activity. Peptide sequences were identified by LC-MS/MS analysis and a molecular docking approach was used to predict their ability to interact with Keap1, one of the key proteins of the Keap1/Nrf2 pathway, the major system involved in redox regulation. Peptides showing a high score of interaction were selected and tested for their antioxidant properties in a cellular environment using the Caco-2 cell line and examined for their capability to defend cells against oxidative stress. Our results indicate that several of the selected peptides were indeed able to activate the Keap1/Nrf2 pathway with the consequent overexpression of antioxidant and phase II enzymes

Tensor Analyzing Powers for Quasi-Elastic Electron Scattering from Deuterium

We report on a first measurement of tensor analyzing powers in quasi-elastic electron-deuteron scattering at an average three-momentum transfer of 1.7 fm$^{-1}$. Data sensitive to the spin-dependent nucleon density in the deuteron were obtained for missing momenta up to 150 MeV/$c$ with a tensor polarized $^2$H target internal to an electron storage ring. The data are well described by a calculation that includes the effects of final-state interaction, meson-exchange and isobar currents, and leading-order relativistic contributions.Comment: 4 pages, 3 figure