An Approach to Relate Viewpoints and Modeling Languages

The architectural design of distributed enterprise applications from the viewpoints of different stakeholders has been proposed for some time, for example, as part of RM-ODP and IEEE 1471, and seems now-a-days to gain acceptance in practice. However, much work remains to be done on the relationships between different viewpoints. Failing to relate viewpoints may lead to a collection of viewpoint models that is inconsistent, and may therefore lead to an incorrect implementation. This paper defines an approach that helps designers to relate different viewpoints to each other. Thereby, it helps to enforce the consistency of the overall design. The results of this paper are expected to be particularly interesting for Model Driven Architecture (MDA) projects, since the proposed models can be used for the explicit definition of the models and relationships between models in an MDA trajectory

Effective extraction and filtering of frequency components in physiological signals using sum-of-sinusoids modelling

In biological signal processing, modelling and extraction of specific frequency components constitute an important procedure for filtering signal components of interest as well as artefact removal. Under some interference scenarios, a satisfactory elimination of artefacts from the signal must be even performed by subtraction of an artefact waveform model or template, rather than the use of linear band-pass filters. That is the case of the gradient artefact induced in the EEG within the fMRI scanner, which cannot be characterized by a specific bandwidth or spectral content. This paper presents a simple and accurate approach based upon sum-of-sinusoids modelling for signal and artefact frequency components representation in physiological signals. According to the proposed method, each signal frequency component is approximated as a sinusoid, whose amplitude and phase parameters are estimated by making use of the Discrete Fourier Transform (DFT). The proposed approach reveals to perform an effective modelling and extraction of ECG signal components as well as underlying gradient artefacts in the EEG signal

Utilising PRA to develop a postgraduate qualification in visual impairment studies

This article reports on the study that focused on the utilisation of a Participatory Reflection and Action (PRA) methodology in order to develop a postgraduate qualification in visual impairment studies. The broader project aims to create ways in which learners with visual impairment can be included in South African schools. In lieu of this, it has therefore come to our attention that teachers of these learners in full service schools (FSS) and special needs schools may require appropriate education and training so as to effectively work with learners with visual impairment. For our exploration, we followed a case study design and utilised PRA approach as the main data generation source in the five provinces that were involved in the study. The sample involved 255 teachers in full service and special needs schools and 50 expert stakeholders in the field of visual impairment. To most teachers, being involved in PRA-based workshops was somewhat new, thus, our study found that teachers have varying views in terms of them (teachers) being part of PRA-based workshops. Although most of them commend the process of these workshops, others feel that there could be an opportunity for improvement

Changes in the carbon balance of tropical forest: evidence from long-term plots

The role of the world’s forests as a “sink” for atmospheric carbon dioxide is the subject of active debate. Long-term monitoring of plots in mature humid tropical forests concentrated in South America revealed that biomass gain by tree growth exceeded losses from tree death in 38 out of 50 neotropical sites. These forest plots have accumulated 0.71 + 0.34 tons of carbon per hectare per year in recent decades. The data suggest that neotropical forests may be a significant carbon sink, reducing the rate of increase in atmospheric CO2

Improving manual assembly lines devoted to complex electronic devices by applying Lean tools

Manual assembly lines are usually deeply studied before implementation. Nevertheless, several problems upsurge when the product needs to be slightly changed. This is very common in complex electric and electronic devices usually produced in small batches, where the customers are demanding more and more features and the product needs to be continuously updated. However, these updates sometimes create huge difficulties for the previously installed assembly line, generating as well, line unbalancing and wastes of time regarding the initial situation. In this paper, a deep study of an adjusted assembly line of electronic devices was carried out using Value Stream Mapping (VSM) method to fully understand and document the different tasks and operations. The Lean Line Balancing (LLB) was also applied in order to reduce the line bottleneck by balancing the Task Time (TT) of each workstation so that there are no delays, and nobody is overburden with their task. Standardized processes and standardized work were also applied. During the line layout development stage, assembly fixtures, wastes reductions and visual management techniques were applied as well, different concepts were generated and, finally, the best solutions were selected. Throughout the study, many benefits for the studied manual assembly line were found, which can be considered as a strong motivation to apply Lean Manufacturing (LM) tools for better line efficiency and production rate.info:eu-repo/semantics/publishedVersio

Locking the GFP Fluorophore to Enhance Its Emission Intensity

Funding Information: Thanks are due to the University of Aveiro, FCT/MEC, Centro 2020 and Portugal2020, the COMPETE program, and the European Union (FEDER program) via the financial support to the LAQV-REQUIMTE (UIDB/50006/2020 and UIDP/50006/2020), to the CICECO-Aveiro Institute of Materials (UID/CTM/50011/2019, UIDB/50011/2020 and UIDP/50011/2020), financed by national funds through the FCT/MCTES, to the Portuguese NMR Network. SG is supported by national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. JRMF. Thanks FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for her PhD grant (UI/BD/151272/2021). Publisher Copyright: © 2022 by the authors.The Green Fluorescent Protein (GFP) and its analogues have been widely used as fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the fluorescence of the GFP is not emissive when isolated in solution, outside the protein environment. The most accepted explanation is that the quenching of the fluorescence results from the rotation of the aryl–alkene bond and from the Z/E isomerization. Over the years, many efforts have been performed to block these torsional rotations, mimicking the environment inside the protein β-barrel, to restore the emission intensity. Molecule rigidification through chemical modifications or complexation, or through crystallization, is one of the strategies used. This review presents an overview of the strategies developed to achieve highly emissive GFP chromophore by hindering the torsional rotations.publishersversionpublishe