Investigação das atividades anti-inflamatória e antiproliferativa de nanopartículas de ouro

Orientador : Daniela Cabrini de AlmeidaCoorientador: Priscila Lúcia PawloskiMonografia (graduação) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Curso de Graduação em BiomedicinaInclui referência

Evaluation of anthropometric facial landmarks in woman with Blepharophimosis, Ptosis, and Epicanthus Inversus Syndrome (BPES)

Introduction: Blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) is a syndrome easily recognized by facial appearance. In this sense, the facial anthropometry is a simple and non-invasive way to evaluate the morphology of the facial surface of individuals, thus, defining the craniofacial dimensions. Objective: To evaluate the facial anthropometric measurements of a Caucasian female, aged 20 years, diagnosed with BPES and to compare these measures with the values described in the literature for non-syndromic woman.Material and methods: This research is an observational study of a Caucasian female, aged 20 years, who was diagnosed with BPES. Frontal photographs were taken, and the images analyzed by nine researchers calibrated in Image J® software. The facial measurements evaluated were head, face, orbits, nose, and labio-oral region and were compared with non-syndromic woman. Results: All vertical and horizontal face measurements were higher than that of otherfemales from Caucasian groups. BPES woman also presented bilateral ptosis and the main differences appear in the region of the orbits. Conclusion: The anthropometric facial analysis of BPES woman showed a significant change in the facial landmarks.Introduction: Blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) is a syndrome easily recognized by facial appearance. In this sense, the facial anthropometry is a simple and non-invasive way to evaluate the morphology of the facial surface of individuals, thus, defining the craniofacial dimensions. Objective: To evaluate the facial anthropometric measurements of a Caucasian female, aged 20 years, diagnosed with BPES and to compare these measures with the values described in the literature for non-syndromic woman.Material and methods: This research is an observational study of a Caucasian female, aged 20 years, who was diagnosed with BPES. Frontal photographs were taken, and the images analyzed by nine researchers calibrated in Image J® software. The facial measurements evaluated were head, face, orbits, nose, and labio-oral region and were compared with non-syndromic woman. Results: All vertical and horizontal face measurements were higher than that of otherfemales from Caucasian groups. BPES woman also presented bilateral ptosis and the main differences appear in the region of the orbits. Conclusion: The anthropometric facial analysis of BPES woman showed a significant change in the facial landmarks

Educomunicação, Transformação Social e Desenvolvimento Sustentável

Esta publicação apresenta os principais trabalhos dos GTs do II Congresso Internacional de Comunicação e Educação nos temas Transformação social, com os artigos que abordam principalmente Educomunicação e/ou Mídia-Educação, no contexto de políticas de diversidade, inclusão e equidade; e, em Desenvolvimento Sustentável os artigos que abordam os avanços da relação comunicação/educação no contexto da educação ambiental e desenvolvimento sustentável

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Investigação das atividades anti-inflamatória e antiproliferativa de nanopartículas de ouro

Orientador : Daniela Cabrini de AlmeidaCoorientador: Priscila Lúcia PawloskiMonografia (graduação) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Curso de Graduação em BiomedicinaInclui referência

Análise da despigmentação da pele causada por monobenzona em camundongos nocautes para os receptores das cininas

Orientadora: Profa Dra Daniela de Almeida CabriniCoorientador: Prof. Dr. Michel Fleith OtukiDissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Farmacologia. Defesa : Curitiba, 30/11/2021Inclui referênciasResumo: As alterações na pigmentação da pele podem ser o resultado de anormalidades em um determinado estágio da formação da melanina, destruição dos melanócitos ou degradação defeituosa da melanina. Estudos anteriores demonstraram que todos os constituintes do sistema das cininas são expressos constitutivamente na pele normal e patológica, sugerindo que os peptídeos cininas podem estar envolvidos em importantes funções primárias da pele e eventos patológicos. No entanto, não há estudos relatando a participação dos receptores de cininas no processo de despigmentação da pele. Neste contexto, o presente estudo foi desenhado com o objetivo de determinar o papel dos receptores das cininas no modelo do vitiligo induzido por monobenzona. Os resultados obtidos mostraram que camundongos knockout para os receptores de cininas B1 e B2, assim como os camundongos knockout para os receptores duplos de cininas, apresentaram despigmentação local da pele maior do que o tipo selvagem (WT) após aplicação tópica diária de creme de monobenzona a 40% (MBZ) por 50 dias. Da mesma forma, foi observado nível mais baixo de conteúdo de melanina na epiderme de camundongos knockout para o receptor B1 ou B2 tratados com MBZ. Digno de nota, os camundongos KOB1B2R exibiram níveis diminuídos de melanina na pele normal, e o tratamento com MBZ não alterou isso. Comparativamente, a ablação genética dos receptores de cininas B1 e B2 levou à redução da geração de EROs em animais tratados com MBZ. Novamente, os camundongos KOB1B2R apresentaram níveis mais baixos de EROs em condições fisiológicas da pele. A deficiência dos receptores de cinina B1 e B2 mostrou aumento do infiltrado de células dérmicas na pele com vitiligo. Da mesma forma, a ausência do receptor B1 foi associada ao maior acúmulo de IL-4, IL-6 e IL-17 em animais tratados com MBZ. Relevantemente, a ausência de ambos os receptores B1 e B2 foi associada a um efeito protetor, pois preveniu o aumento nos níveis de citocinas induzido pelo tratamento com MBZ.Abstract: Changes in skin pigmentation can be the result of abnormalities at a certain stage of melanin formation, destruction of melanocytes or a defective degradation of melanin. Previous studies have shown that all kinin system constituents are constitutively expressed in the normal and pathological skin, suggesting that kinin peptides may be involved in important primary function of the skin and pathological events. However, there are no studies reporting the participation of the kinin receptors in skin depigmentation process. In this context, the present study was designed in order to determine the role of kinin receptors in the monobenzone-induced vitiligo-like model. The obtained results showed that kinin B1 and B2 receptor knockout mice, as well as double kinin receptors knockout mice presented higher local skin depigmentation than wild type (WT) after daily topical application of 40% monobenzone (MBZ) cream over 50 days. Similarly, was observed lower level of melanin content in epidermis of B1 receptor or B2 receptor knockout mice treated with MBZ. Of note, KOB1B2R mice displayed decreased levels of melanin in the normal skin, and MBZ treatment did not change it. Comparably, the genetic ablation of B1 and B2-kinin receptors leads to reduced ROS generation in animals MBZ- treated. Again, the KOB1B2R mice presented lower ROS levels in physiological skin conditions. Both kinin B1 and B2 receptor deficiency showed increased dermal cell infiltrate in vitiligo skin. In the same way, lack of the B1 receptor was associated with greater accumulation of IL-4, IL-6 and IL-17 in animals treated with MBZ. Relevantly, the absence of both B1 and B2 receptors was associated with a protective effect, as it prevented the increase in cytokine levels induced by MBZ treatment

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data