Occasional Finding Of Neurological Disorders During Children Hearing Loss Evaluation Using The Abr

One of the most important applications of the Brainstem evoked response audiometry (ABR) is in the evaluation of hearing loss in children. Today the ABR is also indicated in the screening of cochleovestibular syndromes to detect retrocochlear lesions, to monitor patients in a coma (brain death), in monitoring the brainstem during skull base surgery, etc. Among the many BERA qualities, is its capacity to evaluate the neurophysiologic integrity of the auditory brainstem pathway. In doing so, sometimes while evaluating hearing function in children we are faced with ABR waves that suggest the presence of retrocochlear lesions (trace asymmetry, increased interpeak intervals), many times confirmed through image studies. These cases are seen as occasional findings of neurologic disorders during children hearing loss evaluation. In this study we report 2 cases of neurologic disorders diagnosed with the use of the ABR to evaluate hearing loss in children. © Revista Brasileira de Otorrinolaringologia. All Rights reserved.733424428Jewett, D.L., Romano, M.N., Williston, J.S., Human auditory evoked potentials: Possible brain stem components detected on the scalp (1970) Science, 167 (924), pp. 1517-1518Starr, A., Hamilton, A.E., Correlation between confirmed sites of neurological lesions and abnormalities of far-field auditory brainstem responses (1976) Electroencephalogr Clin Neurophysiol, 41 (6), pp. 595-608Levine, R.A., Gardner, J.C., Fullerton, B.C., Stufflebeam, S.M., Carlisle, E.W., Furst, M., Effects of multiple sclerosis brainstem lesions on sound lateralization and brainstem auditory evoked potentials (1993) Hear Res, 68 (1), pp. 73-88Moller, A.R., Jannetta, P.J., Auditory evoked potentials recorded intracranially from the brain stem in man (1982) Exp Neurol, 78 (1), pp. 144-157Hashimoto, I., Ishiyama, Y., Yoshimoto, T., Nemoto, S., Brain-stem auditory-evoked potentials recorded directly from human brain-stem and thalamus (1981) Brain, 104 (PART 4), pp. 841-859Anson, B., Donaldson, J., (1981) Surgical anatomy of the temporal bone, , 3rd ed. Philadelphia: W.B. Saunders;Sousa LCA, Piza MRT, Costa SS. Poster: Brainstem Evoked Auditory Potential (ABR) in Neurology and Neurosurgery: 99th Meeting of the American Academy of Otolaryngology- Head and Neck Surgery. New Orleans, USA, Setembro1995Sousa, L.C.A., Piza, M.R.T., Costa, S.S., Diagnosis of Menières Disease: Routine and Extended Tests (2002) Otolaryngol Clin North Am, , JuneMarangos, N., Maier, W., Merz, R., Laszig, R., Brainstem response in cerebellopontine angle tumors (2001) Otol Neurotol, 22 (1), pp. 95-99Haapaniemi, J., Laurikainen, E., Jahansson, R., Rinne, T., Varpula, M., Audiovestibular findings and location of na acoustic neuroma (2000) Eur Arch Otorhinolaryngol, 257 (4), pp. 237-241Costa, S.S., Sousa, L.C.A., Cruz, O.L.M., Colli, B.O., Andrade, M.J., Rollin, G.A.F.S., Schwannoma vestibular: Apresentação atípica (1995) J Bras Neurosurg, 6 (2), pp. 41-48Sousa LCA, Piza MRT, Costa SS, Ferez M, Colli BO. Electrophysiologic Monitoring (ABR) of Coma Status,: 99th Meeting of the American Academy of Otolaryngology. Head and Neck Surgery, New Orleans, USA1995Coser, P.L., Menon, A.D., Electrophysiological study of auditory pathways and the vestibular system in tumor pathology of the 8th cranial nerve and of the cerebellopontile angle (1981) Rev Laryngol Otol Rhinol (Bord), 102 (5-6), pp. 239-24

Cutinase activity in supercritical and organic media: water activity, solvation and acid–base effects

We performed a comparative study on the activity of Fusarium solani pisi cutinase immobilized on zeolites NaA and NaY, in n-hexane, acetonitrile, supercritical ethane (sc-ethane) and sc-CO2, at two different water activity (aW) values set by salt hydrate pairs in situ and at acid–base conditions fixed with solid-state buffers of aqueous pKa between 4.3 and 10.6. The reaction studied was the transesterification of vinyl butyrate by (R,S)-2-phenyl-1-propanol. The transesterification activity of cutinase was highest and similar in sc-ethane and in n-hexane,about one order of magnitude lower in acetonitrile and even lower in sc-CO2. Activity coefficients (γ) generated for the two substrates indicated that they were better solvated in acetonitrile and thus less available for binding at the active site than in the other three solvents. γ data also suggested higher reaction rates in sc-ethane than in n-hexane, as observed, and provided evidence for a direct negative effect of sc-CO2 on enzyme activity. Manipulation of the acid–base conditions of the media did not afford any improvement of the initial rates of transesterification relative to the blanks (no added acid–base buffer, only salt hydrate pair), except in the case of cutinase immobilized on zeolite NaA in sc-ethane at aW = 0.7. The poor performance of the blank in this case and the great improvement observed in the presence of a basic buffer suggest a deleterious acidic effect in the medium which, an experiment without additives confirmed, was not due to the known acidic character of the salt hydrate pair used to set aW = 0.7. In acetonitrile, increasing aW was accompanied by a decrease in initial rates of transesterification, unlike in the other solvents. There was considerable hydrolysis in acetonitrile, where initial rates of hydrolysis increased about 20-fold from aW = 0.2 to 0.7. Hydrolysis was less pronounced in sc-ethane and in n-hexane, and only at aW = 0.7, and in sc-CO2 butyric acid was detected only at very long reaction times, in agreement with a generally low catalytic activity. Cutinase enantio-selectivity towards the alcohol substrate was low and unaffected by any manipulation of medium conditions.This work has been supported by Fundação para a Ciência e Tecnologia (FCT, Portugal) through the contracts PRAXIS/PBIO/14314/1998 and POCTI/35429/QUI/2000 and the grant PRAXIS XXI/BD/21615/99 (S. Garcia), and by FEDER.We thank Ricardo Baptista for help in the production of cutinase

Pragas da soja no Brasil e seu manejo integrado.

Pragas da soja; Pragas que atacam as folhas: Anticarsia gemmatalis (Lep.: Noctuidae); Pseudoplusia includens (Lep.: Noctuidae); Coleopteros desfolhadores; Outros organismos que atacam as folhas; Pragas que atacam vagens e graos: Percevejos sugadores de sementes; Lagartas das vagens; Brocas das vagens; Pragas que atacam plantulas, hastes e peciolos: Sternechus subsignatus (Col.: Curculionidae); Elasmopalpus lignosellus (Lep.: Pyralidae); Epinotia aporema (Lep.: Tortricidae); Outros insetos que atacam plantulas, hastes e peciolos; Pragas que atacam as raizes da soja: Coros; Percevejos-castanho-da-raiz; Outros insetos que atacam as raizes; Inimigos naturais das pragas da soja; Predadores: Hemipteros; Coleopteros; Parasitoides: Parasitoides de lagartas; Parasitoides de percevejos; Entomopatogenos: Virus; Fungos; Amostragem das pragas; Controle integrado e nivel de acao; Controle integrado das pragas que atacam as folhas: Anticarsia gemmatalis; Pseudoplusia includens e outros Plusiinae;; Coleopteros desfolhadores; Outros organismos que atacam as folhas; Controle integrado das pragas que atacam as vagens e graos: Percevejos; Lagarta-das-vagens; Broca-das-vagens; Controle integrado das pragas que atacam plantulas, hastes e peciolos: Sternechus subsignatus; Epinotia aporema; Controle integrado das pragas que atacam as raizes: Coros; Percevejo-castanho-da-raiz.bitstream/item/128003/1/ID-6173.pd

Z-score mapping for standardized analysis and reporting of cardiovascular magnetic resonance modified Look-Locker inversion recovery (MOLLI) T1 data: normal behavior and validation in patients with amyloidosis

BACKGROUND: T1 mapping using modified Look-Locker inversion recovery (MOLLI) provides quantitative information on myocardial tissue composition. T1 results differ between sites due to variations in hardware and software equipment, limiting the comparability of results. The aim was to test if Z-scores can be used to compare the results of MOLLI T1 mapping from different cardiovascular magnetic resonance (CMR) platforms. METHODS: First, healthy subjects (n = 15) underwent 11 combinations of native short-axis T1 mapping (four CMR systems from two manufacturers at 1.5 T and 3 T, three MOLLI schemes). Mean and standard deviation (SD) of septal myocardial T1 were derived for each combination. T1 maps were transformed into Z-score maps based on mean and SD values using a prototype post-processing module. Second, Z-score mapping was applied to a validation sample of patients with cardiac amyloidosis at 1.5 T (n = 25) or 3 T (n = 13). RESULTS: In conventional T1 analysis, results were confounded by variations in field strength, MOLLI scheme, and manufacturer-specific system characteristics. Z-score-based analysis yielded consistent results without significant differences between any two of the combinations in part 1 of the study. In the validation sample, Z-score mapping differentiated between patients with cardiac amyloidosis and healthy subjects with the same diagnostic accuracy as standard T1 analysis regardless of field strength. CONCLUSIONS: T1 analysis based on Z-score mapping provides consistent results without significant differences due to field strengths, CMR systems, or MOLLI variants, and detects cardiac amyloidosis with the same diagnostic accuracy as conventional T1 analysis. Z-score mapping provides a means to compare native T1 results acquired with MOLLI across different CMR platforms

Susceptibility testing of Candida albicans and Candida glabrata to Glycyrrhiza glabra L.

Medicinal plants and their botanical formulations have gained a pivotal attention among scientific researchers mainly due to its action as health promoters. Licorice (Glycyrrhiza glabra L.) constitutes a great example, with an increasingly evidenced antimicrobial potential. Opportunistic yeast infections constitute an alarming public health problem, highly exacerbated by the inefficacy of antifungal drugs and the increase of drug-resistant microorganisms, being Candida species one of the most common invaders. The present work aims to assess Candida glabrata and Candida albicans susceptibility to G. glabra methanol: water extract by using flow cytometry and transmission electron microscopy techniques. After 5 minutes, licorice extract (1.5 mg/mL) altered Candida membrane potential. Within an hour, it induced primary damages on Candida species cells, causing cell cytoplasm disorganization with high evidence of cell membrane invaginations, making cells turgid. Thus, based on the current findings, licorice extract seems to be a promising anti-Candida agent, without presenting any toxic potential at the effective concentrations used.The authors are grateful to Foundation for Science and Technology (FCT, Portugal) for N. Martins grant (SFRH/BD/87658/2012) and financial support to the research centre CIMO (strategic project PEst-OE/ AGR/UI0690/2014). This work was also supported by the Programa Operacional, Fatores de competitividade – COMPETE and by national funds through FCT – Fundação para a Ciência e a Tecnologia on the scope of the projects FCT PTDC/SAU-MIC/119069/2010, RECI/EBBEBI/0179/2012 and PEst-OE/EQB/LA0023/2013. The authors thank the Project “BioHealth – Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE-07-0124-FEDER-000027, cofunded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER.info:eu-repo/semantics/publishedVersio

The formation of planetary disks and winds: an ultraviolet view

Planetary systems are angular momentum reservoirs generated during star formation. This accretion process produces very powerful engines able to drive the optical jets and the molecular outflows. A fraction of the engine energy is released into heating thus the temperature of the engine ranges from the 3000K of the inner disk material to the 10MK in the areas where magnetic reconnection occurs. There are important unsolved problems concerning the nature of the engine, its evolution and the impact of the engine in the chemical evolution of the inner disk. Of special relevance is the understanding of the shear layer between the stellar photosphere and the disk; this layer controls a significant fraction of the magnetic field building up and the subsequent dissipative processes ougth to be studied in the UV. This contribution focus on describing the connections between 1 Myr old suns and the Sun and the requirements for new UV instrumentation to address their evolution during this period. Two types of observations are shown to be needed: monitoring programmes and high resolution imaging down to, at least, milliarsecond scales.Comment: Accepted for publication in Astrophysics and Space Science 9 figure

Nosology of genetic skeletal disorders: 2023 revision.

The "Nosology of genetic skeletal disorders" has undergone its 11th revision and now contains 771 entries associated with 552 genes reflecting advances in molecular delineation of new disorders thanks to advances in DNA sequencing technology. The most significant change as compared to previous versions is the adoption of the dyadic naming system, systematically associating a phenotypic entity with the gene it arises from. We consider this a significant step forward as dyadic naming is more informative and less prone to errors than the traditional use of list numberings and eponyms. Despite the adoption of dyadic naming, efforts have been made to maintain strong ties to the MIM catalog and its historical data. As with the previous versions, the list of disorders and genes in the Nosology may be useful in considering the differential diagnosis in the clinic, directing bioinformatic analysis of next-generation sequencing results, and providing a basis for novel advances in biology and medicine

Dissecting the contribution of knee joint NGF to spinal nociceptive sensitization in a model of OA pain in the rat

Objective: Although analgesic approaches targeting nerve growth factor (NGF) for the treatment of osteoarthritis (OA) pain remain of clinical interest, neurophysiological mechanisms by which NGF contribute to OA pain remain unclear. We investigated the impact of local elevation of knee joint NGF on knee joint, vs remote (hindpaw), evoked responses of spinal neurones in a rodent model of OA pain. Design: In vivo spinal electrophysiology was carried out in anaesthetised rats with established pain behaviour and joint pathology following intra-articular injection of monosodium iodoacetate (MIA), vs injection of saline. Neuronal responses to knee joint extension and flexion, mechanical punctate stimulation of the peripheral receptive fields over the knee and at a remote site (ipsilateral hind paw) were studied before, and following, intra-articular injection of NGF (10 mg/50 ml) or saline. Results: MIA-injected rats exhibited significant local (knee joint) and remote (lowered hindpaw withdrawal thresholds) changes in pain behaviour, and joint pathology. Intra-articular injection of NGF significantly (P<0.05) increased knee extension-evoked firing of spinal neurones and the size of the peripheral receptive fields of spinal neurones (100% increase) over the knee joint in MIA rats, compared to controls. Intra-articular NGF injection did not significantly alter responses of spinal neurones following noxious stimulation of the ipsilateral hind paw in MIA-injected rats. Conclusion: The facilitatory effects of intra-articular injection of NGF on spinal neurones receiving input from the knee joint provide a mechanistic basis for NGF mediated augmentation of OA knee pain, however additional mechanisms may contribute to the spread of pain to remote site

Low-diffusion Xe-He gas mixtures for rare-event detection: electroluminescence yield

High pressure xenon Time Projection Chambers (TPC) based on secondary scintillation (electroluminescence) signal amplification are being proposed for rare event detection such as directional dark matter, double electron capture and double beta decay detection. The discrimination of the rare event through the topological signature of primary ionisation trails is a major asset for this type of TPC when compared to single liquid or double-phase TPCs, limited mainly by the high electron diffusion in pure xenon. Helium admixtures with xenon can be an attractive solution to reduce the electron diffu- sion significantly, improving the discrimination efficiency of these optical TPCs. We have measured the electroluminescence (EL) yield of Xe–He mixtures, in the range of 0 to 30% He and demonstrated the small impact on the EL yield of the addition of helium to pure xenon. For a typical reduced electric field of 2.5 kV/cm/bar in the EL region, the EL yield is lowered by ∼ 2%, 3%, 6% and 10% for 10%, 15%, 20% and 30% of helium concentration, respectively. This decrease is less than what has been obtained from the most recent simulation framework in the literature. The impact of the addition of helium on EL statistical fluctuations is negligible, within the experimental uncertainties. The present results are an important benchmark for the simulation tools to be applied to future optical TPCs based on Xe-He mixtures. [Figure not available: see fulltext.]