Análise dos Incêndios Florestais num período de 30 anos (1990-2017). Caso de estudo Concelho de Mação (Portugal)

As mudanças climáticas globais são uma temática de grande destaque na nossa sociedade, surgindo, inevitavelmente, associado a esta preocupação, o aumento dos gases de efeito de estufa (GEE) presentes na atmosfera. Segundo Chuvieco et al. (2007) os incêndios florestais são responsáveis por quase 40% das emissões totais de CO2, aumentando significativamente na contribuição dos GEE na atmosfera. Uma forma de compreender os fluxos associados ao carbono é o estudo da quantificação da biomassa florestal, que atua como sumidouro. Ao ocorrer um incêndio florestal, esta dinâmica fica profundamente afetada (Celes et al., 2013).Este artigo é financiado por Fundos Nacionais através da FCT - Fundação para a Ciência e a Tecnologia no âmbito do projeto UID/SOC/04020/2013.info:eu-repo/semantics/publishedVersio

Transformation of UML models for service-oriented software architectures

Comunicação apresentada ao IEEE International Conference and Workshop on the Engineering of Computer Based Systems (ECBS 2005), 12, Washington, 2005.The main aim of this paper is to present how to transform user requirements models into a software architecture for mobile applications. The technique (called "4SRS") is essentially based on the mapping of UML use case diagrams into UML object diagrams. UML sequence, activity, and state diagrams and other artifacts can also be considered within the transformation decisions. The applicability of this technique is illustrated by presenting some results from an e-government mobile application. The development of mobile applications typically follow a service-oriented approach. A service is a software entity running on one or more machines and providing a particular type of function to a priori unknown clients. These services must communicate with each other, whose combination makes up a service-oriented architecture. The communication can involve either simple data passing or it could involve two or more services coordinating some activity. Some means of connecting services to each other is needed, so workflow is a critical part of making services effective. When those services react to changes on user context, application are context-aware. For mobile applications, the definition of the underlying service-oriented software architecture must consider the services themselves as user requirements, as well as the mobile operators entry-points and the final clients interfaces, and use them to characterize the platform.USE-ME.GOV - IST-2002-002294 ; Fundação para a Ciência e a Tecnologia - METHODES (POSI/CHS/37334/2001), STACOS (POSI/CHS/48875/2002)

A demonstration case on the transformation of software architectures for service specification

This paper presents a demonstration case on the successive application of a model-based technique to assist on the refinement of software logical architectures. The technique is essentially based on the transformation of use cases into object diagrams. The applicability of the technique is illustrated by presenting some results from a mobile application. For mobile software, the definition of the underlying service-oriented architecture must consider as user requirements the services themselves, the mobile operators entry points and the final clients interfaces, and use them to characterize the platform. Within the presented demonstration case, the specification of one service of the mobile application was obtained by successively applying the technique.Research funded by FCT and FEDER under project STACOS (POSI/CHS/48875/2002)

A molecularly imprinted sensor for sensitive detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG) oxidative stress biomarker

6th Graduate Student Symposium on Molecular Imprinting6st Graduate Student Symposium on Molecular Imprinting - Kent, 27-28 Agosto 2015Early diagnosis of Oxidative Stress (OS) biomarkers can be used as a crucial tool in cancer prevention, treatment and survival. In this context, 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a repair product of oxidized guanine lesions and has been acknowledged as a suitable biomarker of OS1. Under this scope, a simple and sensitive molecularly imprinted (MIP)-based sensor for detection of urinary 8-OHdG has been designed via electrochemical polymerization. The biomimetic film was assembled in-situ on the gold-modified electrode through electropolymerization of phenol monomer combined with the target molecule 8-OHdG. The electropolymerization of phenol was performed by Cyclic Voltammetry (CV) over the potential range 0.1 to 0.9 V in PBS buffer at pH 7.4, enabling the formation of a non-conductive layer. Several experimental parameters, such as, the initial concentration of the monomer and the ratio template-monomer, have been carefully optimized and the electrochemical performance of the designed MIP sensor was investigated by CV and Electrochemical Impedance Spectroscopy (EIS). In parallel, RAMAN and FTIR spectroscopies comproved the formation of polyphenol films on the electrode surface by electrochemical oxidation of phenol. Our results demonstrated that 8-OHdG molecule was successfully entrapped into the polymeric matrix, enabling a three-dimensional structure with numerous imprinted cavities sites. The developed electrochemical biosensor showed high sensitivity and selectivity towards 8-OHdG over the concentration range [0.1 - 100] pg/ml. Moreover, it was employed to detect 8-OHdG in urine samples as a non-invasive approach to assess the extent of DNA oxidative damage. Overall, this label-free biosensor constitutes a promising low-cost tool to be implemented as an easy-to-use protocol for sensitive detection of 8-OHdG in biological samples

Structure and ligand-based design of P-glycoprotein inhibitors: a historical perspective

Computer-assisted drug design (CADD) is a valuable approach for the discovery of new chemical entities in the field of cancer therapy. There is a pressing need to design and develop new, selective, and safe drugs for the treatment of multidrug resistance (MDR) cancer forms, specifically active against P-glycoprotein (P-gp). Recently, a crystallographic structure for mouse P-gp was obtained. However, for decades the design of new P-gp inhibitors employed mainly ligand-based approaches (SAR, QSAR, 3D-QSAR and phar macophore studies), and structure-based studies used P-gp homology models. However, some of those results are still the pillars used as a starting point for the design of potential P-gp inhibitors. Here, pharmacophore mapping, (Q)SAR, 3D-QSAR and homology modeling, for the discovery of P-gp inhibitors are reviewed. The importance of these methods for understanding mechanisms of drug resistance at a molecular level, and design P-gp inhibitors drug candidates are discussed. The examples mentioned in the review could provide insights into the wide range of possibilities of using CADD methodologies for the discovery of efficient P-gp inhibitors.info:eu-repo/semantics/publishedVersio

Environmental risk assessment in a contaminated estuary: an integrated weight of evidence approach as a decision support tool

Environmental risk assessment of complex ecosystems such as estuaries is a challenge, where innovative and integrated approaches are needed. The present work aimed at developing an innovative integrative methodology to evaluate in an impacted estuary (the Sado, in Portugal, was taken as case study), the adverse effects onto both ecosystem and human health. For the purpose, new standardized lines of evidence based on multiple quantitative data were integrated into a weight of evidence according to a best expert judgment approach. The best professional judgment for a weight of evidence approach in the present study was based on the following lines of evidence: i) human contamination pathways; ii) human health effects: chronic disease; iii) human health effects: reproductive health; iv) human health effects: health care; v) human exposure through consumption of local agriculture produce; vi) exposure to contaminated of water wells and agriculture soils; vii) contamination of the estuarine sedimentary environment (metal and organic contaminants); viii) effects on benthic organisms with commercial value; and ix) genotoxic potential of sediments. Each line of evidence was then ordinally ranked by levels of ecological or human health risk, according to a tabular decision matrix and expert judgment. Fifteen experts scored two fishing areas of the Sado estuary and a control estuarine area, in a scale of increasing environmental risk and management actions to be taken. The integrated assessment allowed concluding that the estuary should not be regarded as impacted by a specific toxicant, such as metals and organic compounds hitherto measured, but by the cumulative risk of a complex mixture of contaminants. The proven adverse effects on species with commercial value may be used to witness the environmental quality of the estuarine ecosystem. This method argues in favor of expert judgment and qualitative assessment as a decision support tool to the integrative management of estuaries. Namely it allows communicating environmental risk and proposing mitigation measures to local authorities and population under a holistic perspective as an alternative to narrow single line of evidence approaches, which is mandatory to understand cause and effect relationships in complex areas like estuaries.info:eu-repo/semantics/publishedVersio

Multidrug resistance reversal effects of aminated thioxanthones and interaction with cytochrome P450 3A4

Aminated thioxanthones have recently been described as dual-acting agents: growth inhibitors of leukemia cell lines and P-glycoprotein (P-gp) inhibitors. To evaluate the selectivity profile of thioxanthones as inhibitors of multidrug resistance (MDR), their interaction with other ABC transporters, which were found to have a strong correlation with multidrug resistance, such as multidrug resistant proteins 1 (MRP1), 2 (MRP2) and 3 (MRP3) and breast cancer resistance protein (BCRP) was also evaluated. The interaction of thioxanthones with cytochrome P450 3A4 (CYP3A4) together with the prediction of their binding conformations and metabolism sites was also investigated. Methods. The UIC2 monoclonal antibody-labelling assay was performed using P-gp overexpressing leukemia cells, K562Dox, incubated with eight thioxanthonic derivatives, in order to confirm their P-gp inhibitory activity. A colorimetric-based ATPase assay using membrane vesicles from mammalian cells overexpressing a selected human ABC transporter protein (P-gp, MRP1, MRP2, MRP3, or BCRP) was performed. To verify if some of the thioxanthonic derivatives were substrates or inhibitors of CYP3A4, a luciferin-based luminescence assay was performed. Finally, the in silico prediction of the most probable metabolism sites and docking studies of thioxanthones on CYP3A4 binding site were investigated. Results. Thioxanthones interacted not only with P-gp but also with MRP and BCRP transporters. These compounds also interfere with CYP3A4 activity in vitro, in accordance with the in silico prediction. Conclusion. Thioxanthonic derivatives are multi-target compounds. A better characterization of the interactions of these compounds with classical resistance mechanisms may possibly identify improved treatment applications.info:eu-repo/semantics/publishedVersio

An assessment of forest fires and CO2 gross primary production from 1991 to 2019 in Mação (Portugal)

Forest-fire rates have increased in Southern European landscapes. These fires damage forest ecosystems and alter their development. During the last few decades, an increase in fast-growing and highly fuel-bearing plant species such as bush, Eucalyptus globulus Labill., and Pinus pinaster Ait. has been observable in the interior of Portugal. This study aims to verify this assumption by the quantification of the biomass carbon sink in the forests of the Mação municipality. Maps of fire severity and forest biomass evolution after a wildfire event were produced for the period of 1991 to 2019. To quantify carbon retention in this region, this evolution was correlated with gross primary production (GPP) on the basis of satellite imagery from Landsat 5, Landsat 8, and MODIS MYD17A2H. Results show that wildfires in Mação increased in area and severity with each passing decade due to the large accumulation of biomass promoted by the abandonment of rural areas. Before the large fires of 2003, 2017, and 2019, carbon rates reached a daily maximum of 5.4, 5.3, and 4.7 gC/m2/day, respectively, showing a trend of forest-biomass accumulation in the Mação municipality.UIDB/04020/2020; UIDB/04292/2020info:eu-repo/semantics/publishedVersio

Protein disulphide isomerase-induced refolding of sonochemically prepared Ribonuclease A microspheres

The present communication describes for the first time the development of Ribonuclease A (RNase A) microspheres using the sonochemical method followed by an enzymatic treatment with protein disulphide isomerase (PDI). Ultrasound application induced changes on the protein physicochemical and biological properties: the enzymatic activity of RNase A was decreased in 35% and the free thiol groups content was significantly increased, probably due to the breakage of protein disulphide bonds and assembly of RNase A monomers. The deconvolution of amide I band, from Fourier Transform Infrared Spectroscopy, showed that the secondary structure of RNase A was slightly changed after microspherization. The PDI application on microspheres promoted the recovery of RNase A biological activity and induced the release of active protein into solution in its native state. These results were promoted by different states of PDI active site: oxidized and reduced, respectively. The PDI aptitude to catalyze the refolding of a protein substrate in the form of spheres is here reported.Margarida Fernandes thanks FCT "Fundacao para a Ciencia e Tecnologia" for providing the grant for PhD studies (SFRH/BD/38363/2007). Helena Ferreira thanks POPH/FSE for co-financing and FCT for fellowship SFRH/BPD/38939/2007

New uses for old drugs: pharmacophore‐based screening for the discovery of P‐glycoprotein inhibitors

P-glycoprotein (P-gp) is one of the best character ized transporters responsible for the multidrug resistance phenotype exhibited by cancer cells. Therefore, there is widespread interest in eluci dating whether existing drugs are candidate P-gp substrates or inhibitors. With this aim, a pharma cophore model was created based on known P-gp inhibitors and it was used to screen a database of existing drugs. The P-gp modulatory activity of the best hits was evaluated by several methods such as the rhodamine-123 accumulation assay using K562Dox cell line, and a P-gp ATPase activ ity assay. The ability of these compounds to enhance the cytotoxicity of doxorubicin was assessed with the sulphorhodamine-B assay. Of the 21 hit compounds selected in silico, 12 were found to significantly increase the intracellular accumulation of Rhodamine-123, a P-gp substrate. In addition, amoxapine and loxapine, two tetracy clic antidepressant drugs, were discovered to be potent non-competitive inhibitors of P-gp, causing a 3.5-fold decrease in the doxorubicin GI50 in K562Dox cell line. The overall results provide important clues for the non-label use of known drugs as inhibitors of P-gp. Potent inhibitors with a dibenzoxazepine scaffold emerged from this study and they will be further investigated in order to develop new P-gp inhibitors.info:eu-repo/semantics/publishedVersio