Estudio de expresión diferencial de genes y distribución de la vinculina en ovario de cerdas

Ponencia publicada en ITEA, vol.104El objetivo de este estudio es analizar las diferencias en la expresión de genes y proteínas en tres estadios reproductivos en cerdas. Con el fin de caracterizar los cambios en los perfiles de expresión, se hibridó RNA de ovario de cerdas en celo, 15 y 45 días de gestación en microchips porcinos. Se detectaron diferencias de expresión en 281 genes (probabilidad posterior <10-11) entre los tres momentos reproductivos analizados en ovario. Uno de estos genes, la vinculina, mostró una expresión 100 veces mayor en celo comparado con 45 días de gestación. Por ello, fue escogido para realizar un análisis de expresión proteica mediante inmunohistoquímica y análisis western blot. Los resultados obtenidos mediante inmunohistoquímica muestran mayor cantidad de vinculina en celo que a 30 días de gestación. Para esta misma proteína, los resultados sugieren la existencia de diferencias significativas entre ovarios de cerdas en celo y a 45 días de gestación mediante la técnica western blotStudy on the differential gene expression and distribution of the vinculin in the ovary of sows The objective of this experiment is to study genes and proteins differing across reproductive stages in swine. RNA from ovary, from sows on heat, 15 and 45 days of pregnancy have been hybridised in porcine oligonucleotide microchips to characterize changes in gene expression profile between different reproductive stages. Expression differences in 281 genes (posterior probability <10-11) have been found between expression at different stages in ovary. One of these genes, vinculin, showed 100 times more expression on heat than at 45 days of pregnancy, so we chose that gene for immunohistochemistry and western blot analysis. On immunohistochemistry we found that ovaries of sows on heat showed stronger vinculin staining than ovaries stroma of sows at 30 days of pregnancy. On western blot, significant differences appeared only between heat and 45 days of pregnancy

Cloud Computing for Climate Modelling: Evaluation, Challenges and Benefits

Cloud computing is a mature technology that has already shown benefits for a wide range of academic research domains that, in turn, utilize a wide range of application design models. In this paper, we discuss the use of cloud computing as a tool to improve the range of resources available for climate science, presenting the evaluation of two different climate models. Each was customized in a different way to run in public cloud computing environments (hereafter cloud computing) provided by three different public vendors: Amazon, Google and Microsoft. The adaptations and procedures necessary to run the models in these environments are described. The computational performance and cost of each model within this new type of environment are discussed, and an assessment is given in qualitative terms. Finally, we discuss how cloud computing can be used for geoscientific modelling, including issues related to the allocation of resources by funding bodies. We also discuss problems related to computing security, reliability and scientific reproducibilityS

Fine scale physical-biological interactions during a shift from relaxation to upwelling with a focus on Dinophysis acuminata and its potential ciliate prey

Wind reversals and quick transitions from relaxation to upwelling in coastal areas cause major changes in water column structure, phytoplankton distribution and dominance, and rates of physiological processes. The cruise "ASIMUTH-Rías" (17–21 June 2013) was carried out in the Galician Rías and adjacent shelf, at the time of a DSP outbreak, to study small-scale physical processes associated with late spring blooms of "D. acuminata" and accompanying microzooplanktonic ciliates with the overall objective of improving predictive models of their occurrence. The cruise coincided with the initiation of an upwelling pulse following relaxation and deepening of a previously formed thin layer of diatoms. A 36-h cell cycle study carried on 18–20 June showed the vertical excursions of the thin layer, mainly delimited by the 13.5–14 °C isotherms and turbulence levels (ε) of 10−8–10−6 m2 s −3, as well as marked changes in phytoplankton composition (increased density and dominance of diatoms). There was no evidence of daily vertical migration of D. acuminata, which remained in the top layer during the cycle study, but the opposite was observed in the ciliate populations. Dinophysis and its potential prey (Mesodinium species) cell maxima overlapped after midday, when the ciliate moved to the surface, suggesting an “ambush” strategy of Dinophysis to catch prey. A remarkable decline (from 0.65 to 0.33 d−1) in division rates (µ) of D. acuminata was associated with increased turbulence (ε 2°C in about 8 h). In contrast, high division rates (µmin ∼ 0.69 d−1) persisted at a mid-shelf station where environmental conditions below the mixed layer were more stable. The onset of upwelling pulses appears to have a double negative effect on the net growth of Dinophysis populations: a direct physical effect due to advective dispersion and an indirect effect, decreased division rates. The latter would be caused by the rapid cooling of the mixed layer, and the increased turbulence at the surface resulting in shear stress to the cells. The short-term impact of upwelling pulses (and the winds promoting it) on the physiology of "Dinophysis" and its ciliate prey, and the role of mid-shelf populations of "Dinophysis" as a relatively undisturbed reservoir for the inoculation of subsequent blooms are discussed.European Commission | Ref. EC FP7-SPACE-2010-1, n. 261860Ministerio de Economía | Ref. CTM2016-75451-C2-2-RInterreg Atlantic Area | Ref. EAPA_182/2016Comisión Nacional de Ciencia y Tecnología Research (CONICYT), Chile | Ref. PAI79160065Comisión Nacional de Ciencia y Tecnología Research (CONICYT), Chile | Ref. REDES17010

Long-term surveillance of the feline leukemia virus in the endangered Iberian lynx (Lynx pardinus) in Andalusia, Spain (2008-2021)

Feline leukemia virus (FeLV) infection is considered one of the most serious disease threats for the endangered Iberian lynx (Lynx pardinus) Over 14 years (2008-2021), we investigated FeLV infection using point-of-care antigen test and quantitative real-time TaqMan qPCR for provirus detection in blood and tissues in lynxes from Andalusia (Southern Spain). A total of 776 samples from 586 individuals were included in this study. The overall prevalence for FeLV antigen in blood/serum samples was 1.4% (5/360) (95% CI: 0.2-2.6), FeLV proviral DNA prevalence in blood samples was 6.2% (31/503) (95% CI: 4.1-8.6), and FeLV proviral DNA in tissues samples was 10.2% (34/333) (95% CI: 7-13.5). From a subset of 129 longitudinally sampled individuals, 9.3% (12/129) PCR-converted during the study period. Our results suggest that FeLV infection in the Andalusian population is enzootic, with circulation of the virus at low levels in almost all the sampling years. Moreover, since only one viremic individual succumbed to the infection, this study suggests that lynxes may therefore control the infection decreasing the possibility of developing a more aggressive outcome. Although our results indicate that the FeLV infection in the Iberian lynx from Andalusia tends to stay within the regressive stage, continuous FeLV surveillance is paramount to predict potential outbreaks and ensure the survival of this population

FILO Ciliophora

Capítulo de libroEl conocimiento de la diversidad de los ciliados planctónicos en aguas de Galicia hasta la fecha es muy limitado. La mayor parte de los estudios publicados solo incluyen grandes grupos o algunas especies dominantes, que clásicamente se identificaban por microscopía óptica adaptada y hoy en día se se usan técnicas moleculares basadas en la amplificación, clonación y secuenciación de regiones del gen codificador del ARNr 18S, que permite la identificación filogenética de las "especies" o OTUs que componen las comunidades de ciliados planctónicos, por ejemplo. La lista añadida incluye 25 familias y 88 géneros y especies, recogidos en el marco de proyectos de observación sistemática del IEO... La clasificación usada se basa en Lynn&Small (2002) y la nomenclatura empleada en WORMS (Warren, 2011). Diferenciación por ambientes pelágicos.En prens

An All-Organic Flexible Visible Light Communication System

Visible light communication systems can be used in a wide variety of applications, from driving to home automation. The use of wearables can increase the potential applications in indoor systems to send and receive specific and customized information. We have designed and developed a fully organic and flexible Visible Light Communication system using a flexible OLED, a flexible P3HT:PCBM-based organic photodiode (OPD) and flexible PCBs for the emitter and receiver conditioning circuits. We have fabricated and characterized the I-V curve, modulation response and impedance of the flexible OPD. As emitter we have used a commercial flexible organic luminaire with dimensions 99 × 99 × 0.88 mm, and we have characterized its modulation response. All the devices show frequency responses that allow operation over 40 kHz, thus enabling the transmission of high quality audio. Finally, we integrated the emitter and receiver components and its electronic drivers, to build an all-organic flexible VLC system capable of transmitting an audio file in real-time, as a proof of concept of the indoor capabilities of such a system.This Project was funded by Comunidad de Madrid through the SINFOTON-CM Research Program (S2013/MIT-2790), and the Spanish Ministry of Economy, the Agencia Estatal de Investigación and European Union's FEDER through the TEC2016-77242-C3-(1-R, 2-R and 3-R) AEI/FEDER, UE Projects

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum