Influence of the soil water content and distribution on both the hydraulic and transpiration performance of 'Manzanilla' olive trees

8 páginas, 4 figuras, 20 referencias.-- VI International Symposium on Irrigation of Horticultural Crops, celebrado del 2-6 de noviembre de 2006 en Viña del Mar, Chile.-- [email protected] work was made with mature 'Manzanilla' olive trees in an orchard of a semi-arid area in southern Spain. Three water treatments were considered: Rainfed, in which the trees had rainfall as the only source of water supply; FAO, in which the trees were under localized irrigation to replace the crop water demand, with some roots left in drying soil; Pond, in which the whole rootzones of the trees were maintained under non-limiting soil water conditions for the whole dry season. Our aim was to obtain information on the mechanisms behind the reduction of transpiration (Ep) in the FAO trees, as compared to the Pond trees. Our results show a near-isohydric behaviour of the FAO trees, i.e. those trees under localized irrigation in which some roots are left in drying showed lower stomatal conductance than the Pond trees in which all roots were in wetted soil. This helped the FAO trees to maintain similar leaf water potentials than the Pond trees. In addition, the FAO trees maintained a constant difference between the water potential of the canopy and that in the soil. This has been described as an isohydrodynamic behaviour, and it is thought to be an improvement over a typically anisohydric behaviour. These mechanisms were behind the similar values of tree hydraulic conductance (K p) found in the FAO and Pond treatments. The Rainfed trees showed lower Kp values because of the low Ep values of those trees, due to the low soil water availability in that treatment. Our results show, however, that the Rainfed trees were able to maintain similar values of Kp all throughout the dry season, which shows that the hydraulic efficiency of the xylem of those trees was little affected by embolism, despite of the high demanding conditions in the area.This work has been funded by the Spanish Ministry of Education and Science, research project No.AGL2006-04666/AGR, and by the EU, research project ref. STREP 023120.Peer Reviewe

Effect of VDR gene polymorphisms on osteocalcin secretion in calcitriol-stimulated human osteoblasts

Effect of VDR gene polymorphisms on osteocalcin secretion in calcitriol-stimulated human osteoblasts.BackgroundThe impact of vitamin D receptor (VDR) gene polymorphisms in bone metabolism remains controversial. Some authors have found a beneficial effect of some VDR gene polymorphisms, while others found no differences, or even a lower bone mass in subjects with the same type of polymorphisms. The aim of this study was to assess if the VDR gene polymorphisms could have an effect on the calcitriol-stimulated osteocalcin in human osteoblasts.MethodsOsteoblasts were obtained from human femoral necks replaced because of osteoarthritis. Bones were cut into pieces of 1 to 2mm and placed in a nylon mesh. After the migration of osteoblasts, the pieces were collected and cultured with different concentrations of calcitriol (10−8, 10−9, and 10−10 mol/L). After 48 hours of incubation with calcitriol, the osteocalcin secreted into the medium (corrected by either total proteins or total DNA content) was measured. The DNA was extracted from the osteoblasts, amplified by polymerase chain reaction (PCR), and analyzed for target sequences sites of the BsmI, ApaI, TaqI, and FokI restriction enzymes.ResultsThe response observed in osteocalcin secretion in the bb or TT genotypes doubled the response observed in the BB or tt genotypes (calcitriol 10−8 and 10−9 mol/L). A slight trend was also observed with the aa genotype. Men showed higher levels of osteocalcin secretion than women. Age did not show any influence in osteocalcin secretion.ConclusionVDR alleles and gender demonstrated an effect on the osteocalcin secretion. BB or tt genotypes, and also the “A” allele, showed the lowest calcitriol-stimulated osteocalcin secretion

A counterfactual impact evaluation of a bilingual program on students’ grade point average at a spanish university

This observational study intends to estimate the causal effects of an English as a Medium of Instruction (EMI) program (as predictor) on students Grade Point Average (GPA) (as outcome) at a particular University in Spain by using a Counterfactual Impact Evaluation (CIE). The need to address the crucial question of causal inferences in EMI programs to produce credible evidences of successful interventions contrasts, however, with the absence of experimental or quasi-experimental research and evaluation designs in the field. CIE approach is emerging as a methodologically viable solution to bridge that gap. The program evaluated here consisted in delivering an EMI program in a Primary Education Teacher Training Degree group. After achieving balance on the observed covariates and recreating a situation that would have been expected in a randomized experiment, three matching approaches such as genetic matching, nearest neighbor matching and Coarsened Exact Matching were used to analyze observational data from a total of 1288 undergraduate students, including both treatment and control group. Results show unfavorable effects of the bilingual group treatment condition. Potential interpretations and recommendations are provided in order to strengthen future causal evidences of bilingual education programs’ effectiveness in Higher Education.This work was supported by the Junta de Andalucía-funded Proyecto de Excelencia: “Análisis y Garantía de Calidad de la Educación Superior Plurilingüe en la Educación Superior de Andalucía [Junta de Andalusia-funded Project of Excelence: Analysis and Warrantee of the Quality of Plurilingual Higher Education in Andalucia] (AGCEPESA; Grant Agreement No. P12-SEJ − 1588)

Coercive and anisotropy fields in patterned amorphous FeSi submicrometric structures

Amorphous FexSi12x films have been prepared on Si substrates in order to fabricate submicrometric magnetic structures with soft magnetic behavior. The magnetic properties compositional dependence of the unpatterned samples has been analyzed to select the Fe content (x50.7) with the lowest coercive and anisotropy fields values. Arrays of Fe0.7Si0.3 lines have been fabricated by electron beam lithography combined with a liftoff technique, with typical dimensions of 200 nm linewidth and 1 mm line spacing. These arrays present coercive fields parallel to the line direction as small as 9 Oe.Peer reviewe

Role of Klotho and AGE/RAGE-Wnt/β-catenin signalling pathway on the development of cardiac and renal fibrosis in diabetes

Fibrosis plays an important role in the pathogenesis of long-term diabetic complications and contributes to the development of cardiac and renal dysfunction. The aim of this experimental study, performed in a long-term rat model, which resembles type 1 diabetes mellitus, was to investigate the role of soluble Klotho (sKlotho), advanced glycation end products (AGEs)/receptor for AGEs (RAGE), fibrotic Wnt/β-catenin pathway, and pro-fibrotic pathways in kidney and heart. Diabetes was induced by streptozotocin. Glycaemia was maintained by insulin administration for 24 weeks. Serum and urine sKlotho, AGEs, soluble RAGE (sRAGE) and biochemical markers were studied. The levels of Klotho, RAGEs, ADAM10, markers of fibrosis (collagen deposition, fibronectin, TGF-β1, and Wnt/β-catenin pathway), hypertrophy of the kidney and/or heart were analysed. At the end of study, diabetic rats showed higher levels of urinary sKlotho, AGEs and sRAGE and lower serum sKlotho compared with controls without differences in the renal Klotho expression. A significant positive correlation was found between urinary sKlotho and AGEs and urinary albumin/creatinine ratio (uACR). Fibrosis and RAGE levels were significantly higher in the heart without differences in the kidney of diabetic rats compared to controls. The results also suggest the increase in sKlotho and sRAGE excretion may be due to polyuria in the diabetic rats

Library of high and mid-resolution spectra in the CaII H & K, Hα, Hβ, NaI D_1, D_2, and HeI D3 line regions of F, G, K and M field stars

In this work we present spectroscopic observations centered in the spectral lines most widely used as optical indicators of chromospheric activity (Hα, Hβ, CaII H & K, and HeI D_3) in a sample of F, G, K and M chromospherically inactive stars. The spectra have been obtained with the aim of providing a library of high and mid-resolution spectra to be used in the application of the spectral subtraction technique to obtain the active-chromosphere contribution to these lines in chromospherically active single and binary stars. This library can also be used for spectral classification purposes. A digital version with all the spectra is available via ftp and the World Wide Web (WWW) in both ASCII and FITS formats

The JAK3Q988P mutation reveals oncogenic potential and resistance to ruxolitinib

T-cell acute lymphoblastic leukemia (T-ALL) arises from the malignant transformation of T-cell progenitors at various differentiation stages. Given that patients who relapse have a dismal prognosis, there is an urgent need to identify the molecular alterations that are present in such patients and promote leukemogenesis to implement personalized therapies with higher efficacy and fewer adverse effects. In the present manuscript, we identified the JAK3Q988P mutation in a T-ALL patient who did not achieve a durable response after the conventional treatment and whose tumor cells at relapse presented constitutive activation of the JAK/STAT pathway. Although JAK3Q988P has been previously identified in T-ALL patients from different studies, the functional consequences exerted by this mutation remain unexplored. Through the combination of different hematopoietic cellular models, we functionally characterize JAK3Q988P as an oncogenic mutation that contributes to leukemogenesis. Notably, JAK3Q988P not only promotes constitutive activation of the JAK/STAT pathway in the absence of cytokines and growth factors, as is the case for other JAK3 mutations that have been functionally characterized as oncogenic, but also functions independently of JAK1 and IL2RG, resulting in high oncogenic potential as well as resistance to ruxolitinib. Our results indicate that ruxolitinib may not be efficient for future patients bearing the JAK3Q988P mutation who instead may obtain greater benefits from treatments involving other pharmacological inhibitors such as tofacitinib.This work was supported in part by funds from Ministerio de Economía y Competitividad (SAF2015‐70561‐R; MINECO/FEDER, EU to J.F.‐P. and M.V.‐M.); Ministerio de Ciencia, Innovación y Universidades (RTI2018‐093330‐B‐I00; MCIU/FEDER, EU to J.F.‐P. and J.S.); Fundación Ramón Areces (CIVP19S7917 to J.F.‐P.); Comunidad de Madrid (B2017/BMD‐3778; LINFOMAS‐CM to J.F.‐P.); Asociación Española Contra el Cáncer (AECC, 2018; PROYE18054PIRI to J.F.‐P.);and Instituto de Investigación Sanitaria Fundación Jiménez Díaz to J.F.‐P.;institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged.S