Effects of some chemical treatments on standard germination, field emergence and vigour in hybrid maize seeds

The main aim of this experiment was to investigate the possible effects of some active ingredients and their combinations, commonly adopted for maize seed treatment in Italian agriculture, on seed germination, vigour (cold test) and field emergence. Among the seed treatments, fludioxonil + metalaxyl-M (non-systemic fungicides) with the insecticide mixture tefluthrin (belonging to the pyrethroids class) and methiocarb (belonging to the carbamates), negatively affected field emergence of hybrid maize seeds under favourable and restricted temperature conditions, and the cold test results. The two insecticides showed a significant synergistic detrimental action compared with the effects of each active ingredient. These results confirm the need for precaution when using new formulations with an insecticide mixture. The cold test was confirmed as an excellent test able to highlight a possible loss of seed vigour, which usually is not evident with a standard germination test, and to estimate seed performance in the field, especially under cold temperatures, as often happens when adopting modern crop management techniques like sod seeding and early sowing at the end of winter

Bleaching techniques impact on some quality parameters in two different cold-pressed oils obtained at farm scale

The consumption of cold-pressed oil, such as linseed (Linum usitatissimum L.) and hemp (Cannabis sativa L.) oil has increased recently. In these oils obtained on the farm, a large number of components able to affect oil quality (mainly chlorophylls) are present. Given this, an experiment was conducted with the following aims: (1) to compare different bleaching methods by applying ultrasound waves and earths in cold-pressed hempseed and linseed oils and (2) to evaluate the effect of different types (acid-activated and natural) and amount (2% and 4%) of earths with different processing temperatures (60 and 80°C) in hempseed oil, utilizing a bleaching method with earths alone. Regarding the linseed oil, any bleaching methodologies adopted must not include ultrasound treatment, as the high temperatures reached determine high levels of oxidation to the detriment of the oil. Concerning the hemp oil, the natural earths, especially at farm level, should be preferred, because in addition to removing chlorophyll satisfactorily, when used at 4% even at the lowest temperature (60°C), an improvement of the stability and nutritional value (Vit. E) of the oil was observed

Yield and Quality of Essential Oils in Hemp Varieties in Different Environments

Due to its possible utilization in cosmetics, medicine and crop protection, as a valuable alternative to petrochemical-derived products, hemp essential oil is now considered a product with high value added and a promising marketing potential. This experiment was conducted with the aim of evaluating the effect of four different locations of Northern Italy during two years (four environments) and three hemp monoecious varieties on the production and quality of essential oils (EOs) obtained by inflorescences harvested at full flowering of female flowers. The highest inflorescence yield was obtained at Maiano 2017, where a superficial groundwater layer (1.5 m) was present, with values that ranged from 1.69 of Fedora to 2.06 t ha 121 of Futura. EOs production ranged between 3.4 and 4.9 L ha 121 , affected mainly by the variety effect. The terpene in EOs, very similar between varieties and environments, was mainly composed of sesquiterpenes (caryophillene and humulene, as the most abundant) rather than monoterpenes (\u3b1-pinene, \u3b2-myrcene and trans-\u3b2-ocimene, in particular). Phytocannabinoids, and in particular cannabidiol (CBD), were not removed from tissues by the steam during hydrodistillation, and if this is confirmed by further experiments, the residual biomass, now considered as waste, could assume significant importance as a source for further utilization

The Performance and Potentiality of Monoecious Hemp (Cannabis sativa L.) Cultivars as a Multipurpose Crop

Given the growing interest in multipurpose hemp crop, eight monoecious cultivars were compared in a two-year trial for quantitative and qualitative yield in a Mediterranean environment characterized by a temperate and humid climate with hot summers. All hemp cultivars were evaluated for yield potential of (i) seed plus stem at seed maturity, and (ii) essential oil yield from inflorescences harvested at full flowering. The second goal was set to test the ability of cultivars to supply new seeds after the removal of inflorescence at full flowering. Among the cultivars, Fedora obtained the best results for seed (0.79 and 0.52 t ha1) and vegetable oil yield (0.17 and 0.09 t ha1) normally and with inflorescence removed plants, respectively. Futura, conversely, showed the best results for inflorescence (3.0 t ha1), essential oil (9 L ha1), and stem yield at seed maturity (8.34 t ha1), as means across the two years. The cultivars studied generally reached the grain-filling stage during a period that was drier and warmer than the average of the same multi-year period, and this negatively affected seed quality. The oil fatty acid composition was mainly composed of polyunsaturated fatty acids (75% on average) and not affected by the cultivar. In conclusion, although the hemp grower should always clearly know the main production objective of the crop, the monoecious cultivars available today allow a multipurpose use of hemp crop, improving the sustainability of the cultivation activity

Oleic acid variation and marker-assisted detection of Pervenets mutation in high- and low-oleic sunflower cross

High-oleic sunflower oil is in high demand on the market due to its heart-healthy properties and richness in monounsaturated fatty acids that makes it more stable in processing than standard sunflower oil. Consequently, one of sunflower breeder's tasks is to develop stable high-oleic sunflower genotypes that will produce high quality oil. We analyzed variability and inheritance of oleic acid content (OAC) in sunflower, developed at the Institute of Field and Vegetable Crops, by analyzing F-1 and F-2 progeny obtained by crossing a standard linoleic and high-oleic inbred line. F-2 individuals were classified in two groups: low-oleic with OAC of 15.24-31.28% and high-oleic with OAC of 62.49-93.82%. Monogenic dominant inheritance was observed. Additionally, several molecular markers were tested for the use in marker-assisted selection in order to shorten the period of detecting high-oleic genotypes. Marker F4-R1 was proven to be the most efficient in detection of genotypes with Pervenets (high-oleic acid) mutation

Sunflower Hybrid Breeding: From Markers to Genomic Selection

In sunflower, molecular markers for simple traits as, e.g., fertility restoration, high oleic acid content, herbicide tolerance or resistances to Plasmopara halstedii, Puccinia helianthi, or Orobanche cumana have been successfully used in marker-assisted breeding programs for years. However, agronomically important complex quantitative traits like yield, heterosis, drought tolerance, oil content or selection for disease resistance, e.g., against Sclerotinia sclerotiorum have been challenging and will require genome-wide approaches. Plant genetic resources for sunflower are being collected and conserved worldwide that represent valuable resources to study complex traits. Sunflower association panels provide the basis for genome-wide association studies, overcoming disadvantages of biparental populations. Advances in technologies and the availability of the sunflower genome sequence made novel approaches on the whole genome level possible. Genotype-by-sequencing, and whole genome sequencing based on next generation sequencing technologies facilitated the production of large amounts of SNP markers for high density maps as well as SNP arrays and allowed genome-wide association studies and genomic selection in sunflower. Genome wide or candidate gene based association studies have been performed for traits like branching, flowering time, resistance to Sclerotinia head and stalk rot. First steps in genomic selection with regard to hybrid performance and hybrid oil content have shown that genomic selection can successfully address complex quantitative traits in sunflower and will help to speed up sunflower breeding programs in the future. To make sunflower more competitive toward other oil crops higher levels of resistance against pathogens and better yield performance are required. In addition, optimizing plant architecture toward a more complex growth type for higher plant densities has the potential to considerably increase yields per hectare. Integrative approaches combining omic technologies (genomics, transcriptomics, proteomics, metabolomics and phenomics) using bioinformatic tools will facilitate the identification of target genes and markers for complex traits and will give a better insight into the mechanisms behind the traits

Inheritance of the high oleic trait and environmental effects on seed fatty acid composition in High Oleic Sunflower(Helianthus annuus L.).

The topic of research presented in this thesis is the evaluation of environmental and maternal effects on oleic acid content and its inheritability in High Oleic Sunflower. The environmental effects have been studied by using two sowing dates in different years. The effects of temperature are considered. Maternal effects have been studied by using reciprocal crosses and backcrosses between two High Oleic inbred lines. Finally, inheritance of high oleic acid content has been studied by analyzing parents, their F1, F2 and partially F3. The main goal of this thesis is to determine that factors affect oleic acid content in HO genotypes and, consequently, to give some indications for HO sunflower breeding procedures

Development and Optimization of an HPLC-PDA Method for the Determination of Major Cannabinoids in Hemp (Cannabis sativa L.) Essential Oil Obtained by Hydrodistillation

The use of hemp (Cannabis sativa L.) essential oil (EO) has shown a significant increase in interest and use during recent years. In this work, a new and simple reversed-phase HPLC with photodiode-array (PDA) detector method has been developed and optimized for the detection and quantification of cannabidiolic acid (CBDA), cannabidiol (CBD), cannabinol (CBN), Δ9-tetrahydrocannabinol (Δ9-THC), and Δ9-tetrahydrocannabinolic acid (THCA). The cannabinoids were extracted from the EO by partition with n-hexane and water, followed by sonication, evaporation to dryness under nitrogen, and reconstitution with methanol:chloroform (9:1, v/v) before HPLC-PDA analysis. The method shows good selectivity and robustness, linearity in the range 0.5–100 mg L−1 with R2 higher than 0.999 for all cannabinoids analyzed, LOD of 0.11–0.16 mg L−1, and LOQ of 0.35–0.48 mg L−1. The recovery was between 78 and 100% and the intra-day and intermediate precision, expressed as relative standard deviation (RSD), was < 4% and 4–10%, respectively

Seed processing and oil quality of Jatropha curcas L. on farm scale: A comparison with other energy crops

There are many examples of small-scale initiatives, based on exploitation of the jatropha-crude oil chain, to provide energy at local level, especially in the rural areas of a developing country, where mechanical pressing is currently themostwidely used process to extract oil fromseed. This study is a comparative analysis of the extraction performance parameters and the quality of the cold pressed oil obtained utilizing a commercial single screw extruder at the farm level for the extraction of Jatropha curcas, sunflower and rapeseed oil. In the case of J. curcas, the best operating results were obtained at a seed feed rate of 25.7 kg/h yielding about 8 kg/h of oil. This result, although satisfactory, was much lower than that of the rape and sunflower seeds. The levels of the main mineral contents (phosphorus, calcium and magnesium) in the jatropha oil, are significantly higher than those specified in the DIN V 51605 standard. Moreover jatropha oil showed high K and Na content (N2 ppm), the elements that promote deposit formations in engines, while these were not detectable in rapeseed and sunflower oil. Preventive measures (optimizing harvest and storage condition) and avoiding an excessive rise in oil temperature (b50 \ub0C) during the extraction process are necessary to improve the quality of the jatropha crude oil. In order to meet the quality standards of the new DIN 51506, in order to reduce the mineral elements content in the jatropha oil (especially P, Ca + Mg) inert additives may be mixed with the oil during the seed processing, but these additives can lower the oxidation stability of the oil, so a stabilizer also needs to be added. Moreover these steps complicate the technological process and lower the commercial value of the oil, clearly demonstrating the advantage of producing oil with low concentration of P, Ca + Mg and K + Na in the first plac