Whole transcriptome analysis of the hippocampus: toward a molecular portrait of epileptogenesis

<p>Abstract</p> <p>Background</p> <p>Uncovering the molecular mechanisms involved in epileptogenesis is critical to better understand the physiopathology of epilepsies and to help develop new therapeutic strategies for this prevalent and severe neurological condition that affects millions of people worldwide.</p> <p>Results</p> <p>Changes in the transcriptome of hippocampal cells from rats subjected to the pilocarpine model of epilepsy were evaluated by microarrays covering 34,000 transcripts representing all annotated rat genes to date. Using such genome-wide approach, differential expression of nearly 1,400 genes was detected during the course of epileptogenesis, from the early events post <it>status epilepticus (SE) </it>to the onset of recurrent spontaneous seizures. Most of these genes are novel and displayed an up-regulation after <it>SE</it>. Noteworthy, a group of 128 genes was found consistently hyper-expressed throughout epileptogenesis, indicating stable modulation of the p38MAPK, Jak-STAT, PI3K, and mTOR signaling pathways. In particular, up-regulation of genes from the TGF-beta and IGF-1 signaling pathways, with opposite effects on neurogenesis, correlate with the physiopathological changes reported in humans.</p> <p>Conclusions</p> <p>A consistent regulation of genes functioning in intracellular signal transduction regulating neurogenesis have been identified during epileptogenesis, some of which with parallel expression patterns reported in patients with epilepsy, strengthening the link between these processes and development of epilepsy. These findings reveal dynamic molecular changes occurring in the hippocampus that may serve as a starting point for designing alternative therapeutic strategies to prevent the development of epilepsy after acquired brain insults.</p

Acoustic image-based damage identification of oxide aluminum grinding wheel during the dressing operation

Abstract Grinding is a finish process of parts that require high precision and tight dimensional tolerance, which owe high value-added. As the grinding process takes place, the cutting surface of the grinding wheel undergoes wear and then its cutting capacity is reduced. On the other hand, the dressing operation is responsible for restoring the cutting surface of the grinding wheel and, therefore, plays a key role in the grinding process. This work aims at obtaining acoustic images of the grinding wheel surface to identify its conditions during the dressing operation. Experimental tests were conducted with a single-point diamond dresser in a surface grinding machine, which was equipped with an oxide aluminum grinding wheel in which specific marks were intentionally made on its surface to simulate damages for identification. An acoustic emission sensor was fixed to the dresser holder and the signal were acquired at 5 MHz. The signal spectrum was investigated and a frequency band was carefully selected, which represented the conditions of grinding wheel surface. The root mean square values were then computed from the raw signal with and without filtering for several integration periods, and the acoustic images obtained. The results show that the proposed technique is efficient to identify the damage on the wheel surface during the dressing operation as well as its location

Directional field-dependence of magnetoimpedance effect on integrated YIG/Pt-stripline system

We investigated the magnetization dynamics through the magnetoimpedance effect in an integrated YIG/Pt-stripline system in the frequency range of 0.5 up to 2.0 GHz. Specifically, we explore the dependence of the dynamic magnetic behavior on the field orientation by analyzing beyond the traditional longitudinal magnetoimpedance effect of the transverse and perpendicular setups. We disclose here the strong dependence of the effective damping parameter on the field orientation, as well as verification of the very-low damping parameter values for the longitudinal and transverse configurations. We find considerable sensitivity results, bringing to light the facilities to integrate ferrimagnetic insulators in current and future technological applications.This research was funded by CNPq grand numbers 304943/2020-7 and 407385/2018-5, Capes grand number 88887.573100/2020-00 and FCT grant number CTTI-31/18-CF(2)