Characteristics of the 50 patients and transplant.

<p>Characteristics of the 50 patients and transplant.</p

Clusters of immunologic variables correlated to aGVHD, cGVHD, and relapse by multivariate analysis.

<p>Clusters of immunologic variables correlated to aGVHD, cGVHD, and relapse by multivariate analysis.</p

Comparison of immunological variables between patients with and without acute GVHD.

<p>Comparison of immunological variables between patients with and without acute GVHD.</p

Univariate analysis of immunological variables associated with relapse.

<p>Univariate analysis of immunological variables associated with relapse.</p

Characteristics of the 50 patients and transplant.

<p>Characteristics of the 50 patients and transplant.</p

Steroid effect on lymphocyte subsets and index of B/T cell neogenesis at day +90.

<p>Steroid effect on lymphocyte subsets and index of B/T cell neogenesis at day +90.</p

Comparison of immunological variables between patients with and without chronic GVHD.

<p>Comparison of immunological variables between patients with and without chronic GVHD.</p

Gene expression profiles analysis of MSCs after co-culturing with CB-CD34+ cells.

<p>(A) Supervised analysis of MSCs-alone vs. MSCs after 10 days of co-culture with CB-CD34+ cells. A heat map of the genes differentially expressed in a statistically significant manner after co-culture (corrected p value <0.05) is plotted. (B) Gene Set Enrichment Analysis (GSEA) of MSCs-alone vs. MSCs maintained for 10 days in co-culture with CB-CD34+ cells. Top 10 gene sets from hallmark gene sets, canonical/KEGG pathways and oncogenic signatures are plotted. Please refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172430#pone.0172430.s013" target="_blank">S4 Table</a> for the complete GSEA details.</p

<i>Ex vivo</i> expansion of CB-CD34+ cells with MSCs.

<p>5×10⁵ CB-CD34+ cells were cultured alone or in presence of a layer of MSCs for 10 days; the fold increase in total cell number was calculated from the original CD34+ cells seeded at day 0. The absolute number of CB-CD34+ cells was measured by flow cytometry. Values came from 5 independent replicates. Abbreviation: CB-alone: number of CB-CD34+ cells after 10 days of single culture; SN-fraction: CB-CD34+ cells in the supernatant (SN-fraction) of the co-cultures with MSCs; AD-fraction: CB-CD34+ cells grown directly in contact with MSCs layer; Total SN+AD: total number of CB-CD34+ cells after co-culture with MSCs.</p

Additional file 4: of In vitro and in vivo single-agent efficacy of checkpoint kinase inhibition in acute lymphoblastic leukemia

Enrichment analysis report: enrichment by process networks. (PDF 35 kb