Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. siRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single-molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.Peer reviewe

Clinical and structural outcome twenty years after repair of isolated supraspinatus tendon tears

BACKGROUND: This study evaluated the clinical and structural outcome 20 years after repair of isolated supraspinatus tendon tears. We hypothesized that the results would deteriorate over time. MATERIALS AND METHODS: For this retrospective multicenter study, 137 patients were recalled for a clinical and imaging assessment. Six patients (4.3%) had died from unrelated causes, 52 (38.0%) were lost to follow-up, and 13 (9.5%) had undergone reoperations. This left 66 patients for clinical evaluation. Radiographs and magnetic resonance imaging were additionally performed for 45 patients, allowing assessment of osteoarthritis, tendon healing, fatty infiltration (FI), and muscle atrophy. RESULTS: The Constant Score (CS) improved from 51.5 ± 14.1 points preoperatively to 71 points (P < .05) with a mean Subjective Shoulder Value (SSV) of 77.2% ± 22%. Tendon discontinuity (Sugaya IV-V) was present in 19 of 45 patients (42 %), and there was advanced FI (Goutallier III-IV) of the supraspinatus in 12 (27%) and of the infraspinatus muscle in 16 (35%). Supraspinatus atrophy was present in 12 patients (28%), advanced arthritis in 6, and cuff tear arthropathy in 12 (30%). The CS and SSV were significantly inferior for shoulders with FI of stages III to IV (P < .05). The CS was lower in cuff tear arthropathy and correlated with infraspinatus FI. CONCLUSIONS: At 20 years after surgical repair of isolated supraspinatus tears, the clinical outcome remains significantly above the preoperative state. FI of the infraspinatus is the most influential factor on long-term clinical outcome

Effects of Operating Parameters on Ionic Liquid Membrane to Remove Humidity in a Green Continuous Process

Membrane processes are promising methods to separate gases from feed streams without phase changing. A hybrid process, the combination of ionic liquids with a ceramic membrane (ILM), has been developed for humidity removal in a green continuous process. This new concept provides a more efficient and available ionic liquid (IL)-based membrane regeneration process, which just switches the moist feed stream to dry air. Furthermore, the ILM presents high stability and mechanical resistance during long-time operation. In addition, the influences of several operating parameters, including flow rate, temperature, absolute pressure, and feed concentration on process efficiency were investigated. The lower inlet flow rate was found to be favorable for drying humid air. Moreover, when the pressure increased, the mass of absorbed water was increased, while the feed concentration had no significant effects on the membrane separation performance. However, the operating temperature had a great effect on humidity removal. It is necessary to note that the processes at room temperature can limit the energy consumption. The absorbing process of ILM remained efficient after several absorption desorption cycles. Therefore, the new ILM hybrid process that has been developed has great potential for consecutive humidity removal processes

Survivorship of Reverse Shoulder Arthroplasty According to Indication, Age and Gender

Background. The indications for reverse shoulder arthroplasty (RSA) have been widely expanded, but only a few studies report the long-term survival of these implants. Our objective was to report the long-term survivorship of a large series of RSAs implanted for different etiologies. Methods. A retrospective multicenter study including all the RSAs was performed in six shoulder-specialized centers with at least 2 years of follow up. We reviewed 1611 RSAs, operated between 1993 and 2010, including 497 cuff-tear arthropathies (CTA), 239 revision RSAs, 188 massive cuff tears (MCT), 185 fracture sequelae (FS), 183 failed previous cuff repairs (FCR), and 142 primary osteoarthritis (POA). The mean follow-up was 5.6 &plusmn; 3.9 years (range 2&ndash;20). Results. Overall, 266 RSAs (16.5%) had at least one complication leading to 64 reoperations (4.0%) and 110 revision surgeries (6.8%). The most frequent complications were infection (3.8%), instability (2.8%), and humerus-related complications (2.8%). At 10 years, the survival without revision surgery was 91.0% in primary RSAs and 80.9% in revision RSAs for failed arthroplasty (p &lt; 0.001). In the primary RSA group, MCT and FCR led to 10-year survivals for over 95% but fracture sequelae and tumors had the lowest 10-year survivals (83.9% and 53.1%). Younger patients had a lower 10-year survival. In revision RSAs, male patients had a significantly lower survival than females (72.3% vs. 84.5% at 10 years, p = 0.020). Discussion. Primary RSA for cuff-deficient shoulders or POA leads to a high 10-year survival, but revision RSA or primary RSA for FS and tumors are at high-risk for revision. Surgeons should be aware of high rates of complications and lower survival rates of RSA in younger patients, in males, and in RSAs for revision surgery

Membrane SF: Hybrid membrane process to remove emerging pollutants

International audienc

Removal of Humidity by Ionic Liquid Filter to Protect Gas Sensors

International audienceAir quality has become a hot issue of common concern, particularly BTEX gases (Benzene, Toluene, Ethylbenzene and Xylene). They often cause serious environmental problems and have negative effect on human health even at very low concentration. Gas sensors have been in use for monitoring flammable as well as toxic gases. Since a few years, metal oxide gas sensor are also used to the control and monitor the air quality The detection of gaseous pollutants under real conditions requires working in a humid environment. It is well known that the humidity reduces the performances of gas sensors, particularly in terms of sensitivity. In this work, we demonstrated the possibility to reduce the humidity impact on a metal oxide sensor by using ionic liquid-based filter, without modification of the sensor sensitivity

Removal of Humidity by Ionic Liquid Filter to Protect Gas Sensors

International audienceAir quality has become a hot issue of common concern, particularly BTEX gases (Benzene, Toluene, Ethylbenzene and Xylene). They often cause serious environmental problems and have negative effect on human health even at very low concentration. Gas sensors have been in use for monitoring flammable as well as toxic gases. Since a few years, metal oxide gas sensor are also used to the control and monitor the air quality The detection of gaseous pollutants under real conditions requires working in a humid environment. It is well known that the humidity reduces the performances of gas sensors, particularly in terms of sensitivity. In this work, we demonstrated the possibility to reduce the humidity impact on a metal oxide sensor by using ionic liquid-based filter, without modification of the sensor sensitivity

Membrane SF: Hybrid membrane process to remove emerging pollutants

International audienc

Neuropathy of the suprascapular nerve and massive rotator cuff tears: a prospective electromyographic study

An association between massive rotator cuff tear (RCT) and suprascapular nerve neuropathy has previously been suggested. The anatomic course of the suprascapular nerve is relatively fixed along its passage. Thus, injury to the nerve by trauma, compression, and iatrogenic reasons is well documented. However, the association between retraction of the RCT and development of neuropathy of the suprascapular nerve remains unclear. We aimed to prospectively evaluate the suprascapular nerve for preoperative neurodiagnostic abnormalities in shoulders with massive RCT