Cathodic stripping voltammetry of peptide complexes

Small peptides containing the histidine residue are considered as models of the metal binding site of bioactive peptides and proteins. The present study has been concerned with the cathodic stripping voltammetry of the copper complexes of imidazole (the parent molecule), and several histidine containing peptides; glycylglycyl-L-histidine (GGH), glycyl-L-histidylglycine (GHG), L-histidylglycine (HG), glycyl-L-histidine (GH), Carnosine (Car) and thyrotropine releasing factor(TRF). Accumulation and reduction mechanisms were further studied by cyclic voltammetry, and indirect cathodic stripping voltammetric method of determining these peptides has been developed. [Continues.

Optimization of Antioxidants with Different Fermentation TIME in the Salvage Industry Infertile Eggs

The eggs infertile is one alternative food to meet the nutritional needs and other benefts of eggs. The study aims to determine the effect of different fermentation time of the optimization efforts antioxidants in infertile eggs hatching the remaining results. The study design used a completely randomized design (CRD) with three treatments and fve replications. Study treatment was 0, 2 and 4 days. Materials used are infertile eggs hatching period of 18 days as many as 45 items. The parameters measured were the antioxidant activity, the concentration of antioxidants and thiobarbituric acid (TBA) and fat content. The results showed that the antioxidant activity in the rest of the infertile eggs hatching industry results highly signifcant effect increases with the length of fermentation consecutive 0 days amounted to 87.44%, amounting to 96.75% 2 days and 4 days amounted to 99.38%. The concentration of antioxidants in infertile eggs hatching salvage industry very signifcantly decreased with increasing fermentation time consecutive 0 days of 11.56%, 2 days of 10.66% and 4 days of 11.11%. TBA value (malonaldehid mg/10 mg) in infertile eggs hatching salvage industry very signifcantly decreased with increasing fermentation time consecutive 0 days of 0,09, 2 days of 0.08 and 4 days of 0.05. The percentage of fat in infertile eggs hatching salvage industry very signifcantly decreases with increasing length of fermentation consecutive days 0 of 11.56%, 2 days of 10.66% and 4 days of 11.11%. Fermentation for 2 days to optimize the rest of antioxidants in the eggs infertile hatching results

Total laparoscopic hysterectomy: an experience at a tertiary care hospital

Background: This study is aimed to review indications, demographic data of patients, clinical outcomes and safety of total laparoscopic hysterectomy.Methods: This is a prospective observational study of total 150 patients who underwent total laparoscopic hysterectomy (TLH) from 1st June 2017 to 30th November 2018 in GMERS Civil Hospital Sola.Results: ~45% patients were between 40-50 years age group; 60% patients had 2 or more deliveries; commonest indication was symptomatic adenomyosis ; uterine size in ~57% of patients were up to 6 weeks; duration of surgery in ~91% of patients <120 minutes; intraoperative blood loss in all cases <200ml; no intra-operative and postoperative complications were encountered.Conclusions: TLH is safe procedure with minimal blood loss, minimal postoperative pain and discomfort and shorter duration of hospital stay when performed via expert hands

Karakteristik Whey Limbah Dangke Dan Potensinya Sebagai Produk Minuman Dengan Menggunakan Lactobacillus Acidophilus FNCC 0051

Dangke whey has not been widely utilized. Dangke whey handling is required for the prevention of environmental pollution, especially in Enrekang. Evaluating the characteristics of dangke whey is the initial step of dangke whey handling. Complete data about the dangke whey would be scientific information for researchers or the public to further the utilization. Nutritional value of dangke whey allows the use of Lactobacillus acidophilus FNCC 0051 to be processed into beverage products. This research objectives were to investigate the characteristics of whey from dangke waste, which were obtained from milk protein separation by using lyophilization of papaya latex, and it also to evaluate the potential of dangke whey as beverage product by using Lactobacillus acidophilus FNCC 0051. A commercial fermented beverages product is used as a comparison, based on the activity of growth/bacteria numbers, lactic acid and pH. Milk was added with enzyme solution (lyophilized latex) on the level of 0.4 to 1% (v/v). The best level was used for the production of the fermented whey beverage products. Whey was mixed with tapioca level of 0, 0.35, 0.7, 1.05, and 1.4%, and was heated by stirring for 5 minutes at temperature of 70oC. The whey was pasteurized at the temperature of 80oC for 30 minutes. The whey was cooled, and inoculated with L.acidophilus at the level of 1, 3, 5, and 7%, and incubated at 37°C for 8, 12, 16, 18, and 24 hours, respectively. The characteristics of whey dangke were as follows: the solid total was 6.95 ± 0.23%, lactic acid was 0.1 ± 0.003%, fat was 0.2 ± 0.05%, protein was 0.63 ± 0.009%, lactose was 5.08 ± 0.009%, pH was 6.31 ± 0.01, and viscocity was 0.19 ± 0.004 poise. Dangke whey is potential as a beverage products with the addition of Lactobacillus acidophilus with quality similar to the commercial fermented beverage after incubated for 16 hours, with the addition of inoculum level of 5% and tapioca level of 0.35% based on the activity of growth, lactic acid and pH

A Study of Online Learning Studies in Natural Sciences Subject in Biology Topic during the Covid-19 Pandemic

Online learning is one of the efforts made by the government in the education sector to prevent the spread of Covid-19 in Indonesia. The purpose of this study is to determine the implementation of the application of online learning to science lessons in biology material during the Covid-19 pandemic. This research method is descriptive. The results showed that the implementation of online learning of Biology Science material class VIII MTs in Gorontalo City in the teacher aspect included strong criteria as seen from 5 indicators, namely online learning tools, online learning processes, student participation in online learning, online practicum implementation and the assessment process. The implementation of online learning on the material of Biology Science class VIII MTs in Gorontalo City on student aspects includes strong criteria as can be seen from 4 indicators, namely the online learning process, student participation in online learning, implementation of online practicum and increasing creativity. Thus, it can be concluded that the implementation of online learning of biology science material class VIII MTs in Gorontalo City is a strong criterion.Abstrak: Pembelajaran daring adalah salah satu upaya yang dilakukan pemerintah sektor pendidikan untuk mencegah penyebaran Covid-19 di Indonesia. Tujuan penelitian ini untuk mengetahui pelaksanaan penerapan pembelajaran daring pada pelajaran IPA materi biologi di masa pandemi Covid-19. Metode penelitian ini deskriptif kulitatif. Hasil penelitian menunjukkan bahwa keterlaksanaan pembelajaran daring materi IPA Biologi kelas VIII MTs di Kota Gorontalo pada aspek guru termasuk kriteria kuat terlihat dari 5 indikator yaitu perangkat pembelajaran daring, proses pembelajaran daring, partisipasi siswa pada pembelajaran daring, pelaksanaan praktikum secara daring dan proses penilaian. Keterlaksanaan pembelajaran daring pada materi IPA Biologi kelas VIII MTs di Kota Gorontalo pada aspek siswa termasuk kriteria kuat terlihat dari 4 indikator yaitu proses pembelajaran daring, partisipasi siswa pada pembelajaran daring, pelaksanaan praktikum secara daring dan meningkatkan kreativitas. Dengan demikian dapat disimpulkan bahwa keterlaksanaan pembelajaran daring materi IPA biologi kelas VIII MTs di Kota Gorontalo termasuk kriteria kuat. &nbsp; &nbsp

Hubungan Karakteristik Individu Perawat dengan Penerapan Prinsip Benar Pemberian Obat di RS PKU Muhamadiyah Yogyakarta Unit II

Beberapa negara mengalami 70% insiden kesalahan pengobatan hingga menimbulkan kecatatan permanen pada pasien. Kesalahan pemberian obat dapat terjadi karena petugas kesehatan belum menerapkan prinsip benar dalam pemberian obat. Beberapa prinsip benar pemberian obat yaitu: tepat dosis, tepat waktu, tepat pasien, tepat dokumentasian, tepat cara, tepat obat. Tujuan penelitian untuk mengetahui hubungan karakteristik perawat dengan penerapan prinsip benar pemberian obat di RS PKU Muhamadiyah Yogyakarta Unit II. Penelitian ini menggunakan data sekunder yang di peroleh pada penelitian sebelumnya dengan menggunakan sampel sebanyak 32 Perawat, dan dilakukan analisis menggunkan chi square dan Fisher excat test. Hasil penelitian menunjukkan karakteristik individu di RS PKU Muhammadiyah Yogyakarta Unit II berdasarkan jenis kelamin perempuan sebanyak 29 (90,7%), berusia 26-35 tahun yaitu 18 (43,8%), lama bekerja karyawan yang baru bekerja &lt; 1 tahun yaitu 22 (68,8%), pendidikan D3 keperawatan yaitu 27 (84,4%). Kesimpulan penelitian ini adalah terdapat hubungan yang signifikan antara jenis kelamin, usia, lama berkerja dan Pendidikan dengan penerapan prinsip benar pemberian obat.Kata Kunci: Prinsip benar; Benar Obat; Perawat; Right principle; True medicine; Nurse

The prevalence of macrovascular complications among diabetic patients in the United Arab Emirates

<p>Abstract</p> <p>Background</p> <p>Diabetes Mellitus (DM) is a major public health problem in the UAE with a prevalence rate reaching 24% in national citizens and 17.4% in expatriates. The aim of this study was to determine the prevalence and risk factors of macrovascular complications among diabetic patients in the Al-Ain district of the United Arab Emirates (UAE).</p> <p>Methods</p> <p>The study was part of a general cross-sectional survey carried out to assess the prevalence of diabetes (DM) complications among known diabetic patients in Al-Ain District, UAE. Patients were randomly selected during 2003/2004. Patients completed an interviewer-administered questionnaire carried out by treating doctors and underwent a complete medical assessment including measurement of height, weight, blood pressure and examination for evidence of macrovascular complications. A standard ECG was recorded and blood samples were taken to document fasting blood sugar, glycosylated haemoglobin (HbA_1C) and lipid profile.</p> <p>Results</p> <p>A sample of 513 diabetic patients was selected with a mean age of 53 years (SD ± 13.01). Overall, 29.5% of DM patients had evidence of macrovascular complications: 11.6% (95% CI: 8.8–14.4) of patients had peripheral vascular disease (PVD), 14.4% (95% CI: 11.3–17.5) had a history of coronary artery disease (CAD) and 3.5% (95% CI: 1.9–5.1%) had cerebrovascular disease (CVD). Of the total population surveyed 35% (95%CI: 30.8–39) had hypertension. The analysis showed that macrovascular complications in diabetic patients were more common among males, increased with age, were more common among hypertensive patients and its prevalence increased steadily with duration of DM.</p> <p>Conclusion</p> <p>Our data revealed a significant association between hypertension and presence of macrovascular disease among diabetic patients. However, the risk of CAD in the UAE was relatively low compared to that seen in patients in other geographical settings. In addition, a lack of correlation between macrovascular disease and glycemic control among patients with DM was observed.</p

Pengaruh Penggunaan Rhizobium Dan Penambahan Mulsa Organik Jerami Padi Pada Tanaman Kedelai Hitam (Glycine Max (L) Merril) Varietas Detam 1

Kedelai hitam (Glycine max (L) Merril) merupakan tanaman asli Asia tropis seperti Asia Tenggara. Dengan menanam kedelai di sawah, dapat mengembalikan kesuburan tanah. Tujuan penelitian ini untuk mempelajari pengaruh aplikasi mulsa organik jerami padi dan berbagai dosis Rhizobium pada pertumbuhan dan hasil tanaman kedelai hitam (Glycine max (L) Merril). Penelitian dilaksanakan di desa Kedungmaling, kecamatan Sooko, Kabupaten Mojokerto. Ketinggian tempat pada lokasi penelitian 33 mdpl dengan suhu harian berkisar antara 27ºC. Lahan yang digunakan merupakan lahan bekas tanaman padi. Penelitian dilaksanakan pada bulan Juli 2013 hingga Oktober 2013. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari 6 perlakuan dan 4 ulangan. Perlakuan tersebut terdiri dari P1=kontrol, P2=Mulsa jerami padi, P3= Rhizobium dengan dosis 5 g kg-1 benih kedelai, P4=Rhizobium dengan dosis 3 gr kg-1 benih kedelai, P5= Mulsa jerami padi dan Rhizobium dengan dosis 5 g kg-1 benih kedelai dan P6= Mulsa jerami padi dan Rhizobium dengan dosis 3 g kg-1 benih kedelai. Dari hasil penelitian menunjukkan pada perlakuan P5 mampu meningkatkan hasil tanaman kedelai hitam varietas Detam 1. Peningkatan hasil pada tanaman kedelai hitam varietas Detam 1 pada perlakuan P5 mencapai 27,38%. Sedangkan perlakuan yang menunjukkan hasil terendah dalam penelitian ini ialah pada perlakuan P1 (kontrol)

Penerapan Model Pembelajaran Kooperatif Tipe Course Review Horay (Crh) Untuk Meningkatkan Hasil Belajar Matematika Siswa Kelas Viii3 SMP Negeri 4 Tapung

This research was classroom action research that aims to improve learning process and mathematics learning outcomes with applied the cooperative learning type of Course Review Horay (CRH). The subject of the research were students of class VIII3 SMP Negeri 4 Tapung in the second semester academic years 2014/2015. There are 24 students in the class, with the heterogenous level. The research are two cycles, each a cycle has four stages, that are planning, implementation, observation, and reflection. Instrument to collect the data are observation and mathematics achivement test. The observation data analyzed by using descriptive and qualitative, and for students' test by using descriptive and quantitative. The qualitative analysis showed an improvement of learning process prior to action to the first cycle and second cycle. The results of this research showed an increase in students achievement from the based-score with the percentage 29,16% to 45,83% on the first test and 70,83% on the second test. Based on the finding can be concluded the implentation of applied the cooperative learning type of Course Review Horay (CRH) to improve students' mathematics achievement at class VIII3 SMP Negeri 4 Tapung in the second semester academic years 2014/2015

Nitric Oxide and Abscisic Acid Mediate Heat Stress Tolerance through Regulation of Osmolytes and Antioxidants to Protect Photosynthesis and Growth in Wheat Plants

Nitric oxide (NO) and abscisic acid (ABA) play a significant role to combat abiotic stress. Application of 100 µM sodium nitroprusside (SNP, NO donor) or ABA alleviated heat stress effects on photosynthesis and growth of wheat (Triticum aestivum L.) plants exposed to 40 °C for 6 h every day for 15 days. We have shown that ABA and NO synergistically interact to reduce the heat stress effects on photosynthesis and growth via reducing the content of H2O2 and thiobarbituric acid reactive substances (TBARS), as well as maximizing osmolytes production and the activity and expression of antioxidant enzymes. The inhibition of NO and ABA using c-PTIO (2-4 carboxy-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and fluridone (Flu), respectively, reduced the osmolyte and antioxidant metabolism and heat stress tolerance. The inhibition of NO significantly reduced the ABA-induced osmolytes and antioxidant metabolism, exhibiting that the function of ABA in the alleviation of heat stress was NO dependent and can be enhanced with NO supplementation.Thus, regulating the activity and expression of antioxidant enzymes together with osmolytes production could act as a possible strategy for heat tolerance