Ratlara Malathion ve rutin uygulamaları sonrası akciğer dokularında metalloproteinaz düzeyleri ile oksidatif stres ve apoptoz belirteçlerinin değerlendirilmesi

Malathion (MLT) is an important environmental pollutant in the organophosphate class. Rutin (RUT), on the other hand, is one of the flavonoid family members whose effectiveness against various toxic agents has been extensively studied. In the present study, the effects of MLT and RUT treatments on oxidative stress, apoptosis and metalloproteinases in lung tissues of rats were investigated. In the study, MDA, GSH, Nrf2, HO-1, MMP2, MMP9 and caspase-3 levels in lung tissues were analyzed by biochemical or RT-PCR method after rats received MLT and/or RUT treatment for 28 days. The data showed that MLT-induced MDA levels decreased after RUT treatment. In addition, it was determined that Nrf2 and HO-1 mRNA transcript levels and GSH levels suppressed by MLT approached the control group levels after RUT treatment. MLT up-regulated the expression of metalloproteinases (MMP2 and MMP9) in lung tissues, while RUT down-regulated the expression of these genes. In addition, it was observed that MLT triggered caspase-3 expression, while RUT exerted an anti-apoptotic effect by suppressing caspase-3. As a result, it was determined that while MLT showed toxic effects in the lung tissues of rats through oxidative stress, apoptosis and metalloproteinases, RUT could alleviate these toxic effects.Malathion (MLT), organofosfat sınıfında önemli bir çevresel kirleticidir. Rutin (RUT) ise çeşitli toksik ajanlara karşı etkinliği yoğun olarak araştırılan flavonoid aile üyelerinden biridir. Bu çalışmada, MLT ve RUT tedavilerinin sıçanların akciğer dokularında oksidatif stres, apoptoz ve metalloproteinazlar üzerine etkileri araştırıldı. Çalışmada, sıçanlara 28 gün MLT ve/veya RUT tedavisi verildikten sonra akciğer dokularındaki MDA, GSH, Nrf2, HO-1, MMP2, MMP9 ve kaspaz-3 seviyeleri biyokimyasal veya RT-PCR yöntemi ile analiz edildi. Veriler, MLT ile indüklenen MDA seviyelerinin RUT tedavisinden sonra düştüğünü gösterdi. Ayrıca Nrf2 ve HO-1 mRNA transkript düzeyleri ile MLT tarafından baskılanan GSH düzeylerinin RUT tedavisi sonrası kontrol grubu düzeylerine yaklaştığı belirlendi. MLT, akciğer dokularında metalloproteinazların (MMP2 ve MMP9) ekspresyonunu yukarı doğru düzenlerken, RUT bu genlerin ekspresyonunu aşağı regüle etti. Ayrıca MLT'nin kaspaz-3 ekspresyonunu tetiklediği, RUT'nin ise kaspaz-3'ü baskılayarak anti-apoptotik etki gösterdiği gözlendi. Sonuç olarak, MLT'nin oksidatif stres, apoptoz ve metalloproteinazlar yoluyla sıçanların akciğer dokularında toksik etkiler gösterirken, RUT'nin bu toksik etkileri azaltabileceği belirlendi

Evaluation of the effects of chrysin on diclofenac-Induced cardiotoxicity in rats by the markers of oxidative stress, endoplasmic reticulum stress and apoptosis

Diklofenak (DF), steroid olmayan anti-inflamatuar ilaçlar arasındadır ve yüksek dozlarda kalp dahil çeşitli dokularda toksik etkiler göstermektedir. Krisin (KRS) antioksidan ve anti-apoptotik gibi birçok yararlı etkiye sahiptir. Sunulan çalışmada DF ile indüklenen kardiyotoksisite üzerine KRS’nin etkileri araştırılmıştır. Bu amaçla Sprague Dawley ratlara DF ve/veya KRS uygulamaları yapıldıktan sonra kalp dokularında oksidatif stres, endoplazmik retikulum stresi ve apoptoz belirteçleri ve serum CK-MB seviyeleri analiz edildi. Elde edilen veriler DF ile indüklenen oksidatif stresin KRS uygulamasından sonra SOD, CAT ve GPx enzimlerinin ekspresyonlarının tetiklenmesi ve GSH seviyelerinin artması ile hafiflediğini ve MDA seviyelerinde azalma meydana geldiğini göstermektedir. Ayrıca ER stres belirteçleri olan ve DF’nin indüklediği ATF-6, PERK, IRE1 ve GRP78 ekspresyonlarının KRS tedavisinden sonra aşağı yönlü düzenlendiği belirlendi. ER stresi ile bağlantılı olarak KRS’nin Bax ve Kaspaz-3 ekspresyonlarını baskıladığı ve Bcl-2 ekspresyonunu arttırdığı da elde edilen veriler arasındadır. Ayrıca kalp hasarının önemli bir göstergesi olan CK-MB seviyelerinin KRS uygulamasından sonra azaldığı görüldü. Sonuç olarak DF’nin neden olduğu kardiyotoksisiteye karşı KRS’nin önemli bir koruma sağladığı tespit edildi.Diclofenac (DF) is among non-steroidal anti-inflammatory drugs and in high doses it has toxic effects on various tissues, including the heart. Chrysin (CRS) has many beneficial effects such as antioxidant and anti-apoptotic. In the present study, the effects of CRS on DF-induced cardiotoxicity were investigated. For this purpose, after DF and/or CRS treatments were applied to Sprague Dawley rats, the markers of oxidative stress, endoplasmic reticulum stress and apoptosis in heart tissues and serum CK-MB levels were analyzed. The data obtained displayed that oxidative stress induced by DF was alleviated by triggering the expression of SOD, CAT and GPx enzymes and increasing GSH levels after CRS administration, and a decrease in MDA levels occurred. It was also determined that the expressions of ATF-6, PERK, IRE1 and GRP78, which are ER stress markers and induced by DF, were downregulated after CRS treatment. Additionally, CRS suppressed Bax and Caspase-3 expressions and increased Bcl-2 expression in connection with ER stress. Another finding of this study demonstrated that CK-MB levels, which are an important indicator of heart damage, decreased after CRS administration. As a result, CRS provided a significant protection against cardiotoxicity caused by DF

The Role of Hematological Parameters in the Diagnosis of Childhood Allergic Conjunctivitis

Objective:We aimed to investigate the parameters of complete blood count and the levels of systemic inflammatory biomarkers in children with allergic conjunctivit and to evaluate their role in diagnosis in this study.Method:We included 71 pediatric patients with allergic conjunctivit diagnosis referred from the ophthalmology outpatient clinics who had sensitivity to at least one allergen and 71 age- and sex-matched healthy controls and compared complete blood count results, immunoglobulin E (IgE), neutrophil/lymphocyte, and platelet/lymphocyte ratios and systemic immune-inflammation index results. We built a multivariate model with correlated results.Results:Eosinophil counts and serum total IgE values were significantly higher in the patient group compared to the control group (p<0.001). Other parameters were not statistically different. 70.4% (n=50) of the patients had seasonal allergic conjunctivit, and 29.6% (n=21) had perennial allergic conjunctivit. In the skin prick tests performed in the patient group, 60.6% (n=43) of the patients had pollen, 54.9% (n=39) mite, 12.7% (n=9) dander, 11.3% (n=8) cockroach, and 4.2% (n=3) had alternaria sensitivities. In the multivariate analysis, every 100-cell increase in eosinophil count increased the hazard ratio of allergic conjunctivitis 1.3 times (95% confidence interval: 1.1-1.5), and every 100-units increase in total IgE levels increased 1.2 times (95% confidence interval: 1.1-1.5).Conclusion:We found no significant relationship between neutrophil/lymphocyte and platelet/lymphocyte ratios, and SII with allergic conjunctivit. Increasing eosinophil count and serum total IgE levels increase the hazard ratio for developing allergic conjunctivit. Pollen sensitivity was the most common factor in the skin test in allergic conjunctivit-diagnosed patients

Effects of sinapic acid on lead acetate-induced oxidative stress, apoptosis and inflammation in testicular tissue

In this study, the effect of lead acetate (PbAc) and sinapic acid (SNP) administration on oxidative stress, apoptosis, inflammation, sperm quality and histopathology in testicular tissue of rats was tried to be determined. PbAc was administered at a dose of 30 mg/kg/bw for 7 days to induce testicular toxicity in rats. Oral doses of 5 and 10 mg/kg/bw SNP were administered to rats for 7 days after PbAc administration. According to our findings, while PbAc administration increased MDA content in rats, it decreased GPx, SOD, CAT activity and GSH content. NF-kB, IL-1β, TNF-α, and COX-2, which are among the inflammation parameters that increased due to PbAc, decreased with the administration of SNP. Nrf2, HO-1, and NQO1 mRNA transcript levels decreased with PbAc, but SNP treatments increased these mRNA levels in a dose-dependent manner. RAGE and NLRP3 gene expression were upregulated in PbAc treated rats. MAPK14, MAPK15, and JNK relative mRNA levels decreased with SNP treatment in PbAc treated rats. While the levels of apoptosis markers Bax, Caspase-3, and Apaf-1 increased in rats treated with PbAc, the level of Bcl-2 decreased, but SNP inhibited this apoptosis markers. PbAc caused histopathological deterioration in testis tissue and negatively affected spermatogenesis. When the sperm quality was examined, the decrease in sperm motility and spermatozoon density caused by PbAc, and the increase in the ratio of dead and abnormal spermatozoa were inhibited by SNP. As a result, while PbAc increased apoptosis and inflammation by inducing oxidative stress in testicles, SNP treatment inhibited these changes and increased sperm quality

The use of inflammatory markers as a diagnostic and prognostic approach in neonatal calves with septicaemia

The objective of this study was to evaluate the usefulness of inflammatory markers as a diagnostic and prognostic approach in neonatal calves with septicaemia. The study material consisted of 13 neonatal calves with septicaemia (septicaemic calves, SC) and ten healthy neonatal calves (control calves, CC). Blood samples were collected for biochemical, haematological and microbiological analyses. In addition, faecal samples were collected for microbiological and virological analyses. Three of neonatal calves with septicaemia were positive for E. coli (E. coli O157 serotype) by microbiological examination, but all neonatal calves with septicaemia were negative for rota- and coronaviruses. By haematological examination, there were no significant differences between SC and CC for white blood cell (WBC) and neutrophil (NEU) counts (P > 0.05). NEU counts were higher on day 0 than on day 15 in SC (P < 0.05). Red blood cell (RBC) counts and packed cell volume (PCV) values were higher on day 0 in the SC than in the CC (P < 0.05). By biochemical analyses, tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), procalcitonin (PCT), haptoglobin (Hp), and fibrinogen (Fb) concentrations were higher on day 0 in the SC than in the CC (P < 0.05). After treatment (on day 15), the serum IL-6, PCT, Hp, and Fb concentrations were significantly decreased in the SC compared to the CC (P < 0.05). The serum iron (Fe) concentrations were lower on day 0 in the SC than in the CC (P < 0.05), and were higher on day 15 than on day 0 in the SC (P < 0.05). The study revealed that inflammatory markers could be used for determining the diagnosis and prognosis in neonatal calves with septicaemia

Dokuz Tip Mizaç Ölçeği Ergen (DTMÖ-E) Formunun Geçerlik- Güvenirliği ve Ergenlerde Dikkat Eksikliği Hiperaktivite Bozukluğu İle Mizaç Tipleri Arasındaki İlişki

The purpose of this study is developing adolescent form (11-16 years old) of Nine Types Temperament Scale (NTTS) depending on Nine Types Temperament Model (Study 1) and evaluating and searching the relationship between Attention Deficit and Hyperactivity Disorder (ADHD) and Types of NTTM (Study 2). Sample of Study I consist of 1240 students who are between 11 and 16 years old. Pilot form of the Scale which consists of 90 items is tested with confirmatory factor analysis and with convergent and discriminant validity and the last version of the measure which consist of 82 items is prepared. Basic Personality Traits Inventory (BPTI) which depends on Fife Factor Model is used for Criterion-related validation. Results show that NTTS-A is valid and reliable measure for evaluating temperament types between 11-16 years adolescents. Their fit index is calculated as χ2 /df &lt;3; SRMR, 0.06; RMSEA, 0.045; CFI, 0.909: TLI, 0901 supported to validity data. Sample of Study II consist of 56 adolescents who are diagnosed with ADHD according to DSM-IV diagnosis criteria and 56 students who does not get diagnosed with ADHD or mental retardation/medical disorder. Temperament types of participants are evaluated by the agency of Study-I and NTTS-A, which is developed appropriate for Turkish culture. Indication of the study shows that there are more NTT7 and NTT8 in NTTM types between adolescents with ADHD than the group, who are not diagnosed with ADHD. As a result, it is discussed that traits which belong to some temperament types could have a predisposing effect on emergence of ADHD, experiencing these traits extreme severely could cause a view similar to ADHD and ADHD which is a neurodevelopmental disease could interact with temperament traits of person, in that way it could illustrate ADHD clinic. Available findings support critics about that students are labelled as ADHD specially in the last years in education environment even if they have mostly not developmental problems and students are diagnosed easily with limited observations. However, it is asserted that diagnosis based on temperament, treatment and psychosocial support programmes could be formed and effects of ADHD, which is a factor interacting with temperament traits, on personality structure of adolescents on their development process, could be predicted. Our study has the feature of being the first measure developing study which is intended to evaluate temperament types of adolescents between 11-16 years old and also it is has the feature of being the first study which searches relationship between NTTM types and ADHD.Bu çalışmanın amacı, Dokuz Tip Mizaç Modeli (DTMM)’ne dayalı olarak erişkinler için geliştirilen Dokuz Tip Mizaç Ölçeğinin (DTMÖ) 11-16 yaş arası ergen formunu (DTMÖ-E) geliştirmek (Çalışma I) ve DTMM tipleri ile Dikkat Eksikliği Hiperaktivite Bozukluğu (DEHB) arasındaki ilişkiyi incelemek ve değerlendirmektir (Çalışma II). Çalışma I’in örneklemi 11-16 yaş aralığındaki 1240 öğrenciden oluşmaktadır. 90 maddeden oluşan ölçeğin pilot formu doğrulayıcı faktör analizi, yakınsak ve ayırma (diskriminant) geçerliği ile test edilmiş ve ölçeğin 82 maddelik son hali hazırlanmıştır. Ölçüt geçerliği için ise Beş Faktör Modeli’ne dayanan Temel Kişilik Özellikleri Envanteri (TKÖE) kullanılmıştır. Sonuçlar, DTMÖ-E’nin 11-16 yaş arası ergenlerin mizaç tiplerini değerlendirmede kullanılabilecek geçerli ve güvenilir bir ölçek olduğunu göstermektedir. Uyum indeksleri χ2 /df &lt;3; SRMR, 0.06; RMSEA, 0.045; CFI, 0.909: TLI, 0901 olarak geçerlik verilerini destekler nitelikte hesaplanmıştır. Çalışma II’nin örneklemi ise DSM-IV tanı ölçütlerine göre DEHB tanısı almış 56 ergen ve mental retardasyon / tıbbi rahatsızlık ve DEHB tanısı bulunmayan 56 öğrenciden oluşmaktadır. Katılımcıların mizaç tipleri, Çalışma-I ile Türk kültürüne uygun olarak geliştirilen DTMÖ-E aracılığıyla değerlendirilmiştir. Çalışmanın bulguları, DEHB’li ergenler arasında DTMM tiplerinden DTM7 ve DTM8’in DEHB tanısı almayan gruba göre daha fazla bulunduğunu göstermektedir. Sonuç olarak, bazı mizaç tiplerine ait özelliklerin DEHB ortaya çıkmasına yatkınlaştırıcı etkisinin olabileceği, bu özelliklerin aşırı şiddette yaşanmasının DEHB benzeri bir görünüm ortaya çıkarabileceği ve nörogelişimsel bir hastalık olan DEHB’in kişinin mizaç özellikleri ile etkileşerek DEHB kliniğini sergileyebileceği tartışılmıştır. Mevcut bulgular, eğitim çevrelerinde öğrencilerin özellikle son yıllarda sıklıkla gelişimsel bir sorun olmadığı halde DEHB olarak etiketlenmesi ve sınırlı gözlemlerle kolay tanı konmasıyla ilgili eleştirileri destekleyebilecek niteliktedir. Bunun yanı sıra DTMM’nin yaklaşımı sayesinde mizaç temelli tanı, tedavi ve psikososyal destek programlarının oluşturulabileceği ve mizaç özellikleriyle etkileşen bir faktör olarak DEHB’in ergenlerin gelişim süreçlerinde kişilik yapısına nasıl bir etkide bulunacağının öngörülebileceği öne sürülmektedir. Çalışmamız, Türkiye’de 11-16 yaş arasındaki ergenlerin mizaç tiplerini değerlendirmeye yönelik ilk ölçek geliştirme çalışması olmanın yanı sıra, DTMM tipleri ile DEHB arasındaki ilişkiyi inceleyen ilk çalışma olma özelliğindedir

Evaluation of arginase activity, nitric oxide and oxidative stress status in sheep with contagious agalactia

It is known that inflammatory organ damages due to various agents, such as microorganisms including mycoplasmas, lead to oxidative stress. Nitric oxide (NO) functions as an antimicrobial agent, and arginase decreases proinflammatory cytokine release. There are very few studies on arginase activity, NO level and oxidative stress status in mycoplasmal infections. Therefore, the aim of this study was to evaluate erythrocyte arginase activity, plasma NO level and oxidative stress status in sheep with contagious agalactia. The study material consisted of 10 healthy sheep and 14 sheep with contagious agalactia characterised by mastitis, arthritis and keratoconjunctivitis. Erythrocyte arginase activity, plasma NO, malondialdehyde (MDA), total oxidant capacity (TOC) and total antioxidant capacity (TAC) levels were measured. Significant decreases in erythrocyte arginase activity and plasma TAC level (P < 0.001), and significant increases in plasma NO, MDA and TOC levels (P < 0.001) were found in the diseased sheep as compared with the healthy animals. This study suggests that contagious agalactia may cause oxidative stress due to increased plasma MDA and TOC levels and decreased plasma TAC levels, and that the decrease in erythrocyte arginase activity and increase in plasma NO level may contribute to the elimination of mycoplasmal agents causing contagious agalactia

A Primary Leiomyosarcoma of the Thyroid Gland: A Case and Literature Review

Introduction: We want to present a rare case of Primary leiomyosarcoma of the thyroid (PLT) gland and review the literature on PLT including the differential diagnoses, pathology, and alternative treatment strategies.Presentation of Case: A 56-year-old man who underwent left side total thyroidectomy with diagnosis of substernal goiter. On pathologic examination, three different pathology clinics had a common opinion that this was a grade 3 pleomorphic sarcoma of thyroid itself. Positron Emission Tomography (PET/CT) obtained one month after surgery displayed no distant metastases. Loco regional radiotherapy (RT) to the thyroid bed was delivered up to a dose of 59.4 Gray (Gy) in 1.8 Gy daily fractions. PET/CT obtained three months after RT showed bilateral multiple lung metastases without loco regional recurrence. The patient received 6 courses of doxorubicin and cyclophosphamide based chemotherapy. A new PET/CT scan showed only two metabolically active metastases on both lungs. Because of disappearance of small metastases, the patient underwent sequential bilateral metastasectomy in one month interval. Pathology results verified the metastases of PLT. The patient is still alive without any signs of disease 6 years after RT and he is the only long surviving case reported up to now.Conclusion: The treatment protocols for PLT have not been well established yet, because of their rareness and poor prognosis. We believe that our case may be directive for PLT treatment