Erratum

Evolution des teneurs en polyamines dans les boutons floraux, les fleurs et les jeunes baies de Vitis villifera L. (cv. Cabernet Sauvignon) atteints d'eutypioseVitis 43 (3), 139-144 (2004

Isolation and characterization of an antifungal agent active against human pathogenic fungi, produced by Pseudomonas fluorescens FSJ-3

An antifungal agent, produced by Pseudomonas fiuorescens strain FSJ-3 originating from Moroccan soil (Fès), was isolated and characterized. It was extracted from Sabouraud's glucose broth culture by n-butanol and purified by gel filtration and silica gel chromatography giving yellow crystals. Its structure was assigned to 1-phenazinecarboxamide by analysing IH and 13 C-NMR and mass spectral data. It shOwed excellent activity against several species ofbacteria, yeasts and human pathogenic filamentous fungi , including Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus and caused morphological modifications on Arthroderma simii hyphae. I-phenazinecarboxamide was non toxicwhen tested on rat leucocytes up to the highest concentration (200 pg/ml).Isolement et caractérisation d'une substance antifongique élaborée par Pseudomonas fluorescens FSJ-3, active contre des champignons pathogènes de l'hommeLa souche FSJ-3 de Pseudomonas fZuorescens douée d'une forte activité antifongique contre les champignons pathogènes de l'homme a été isolée à partir du sol (Fès, Maroc). La substance active, élaborée par cette souche, a été extraite du milieu de culture liquide, avec du n-butanol et purifiée par chromatographie sur gel de filtration et sur gel de silice. Le I-phénazinecarboxamide, obtenu sous forme de cristaux jaunes, a été analysé par RMN -IH et I3C et par spectrométrie de masse. Cette molécule inhibe la croissance de plusieurs bactéries, levures et champignons filamenteux, comme Candida albicans, Cryptococcus neoformans , Aspergillus fumigatus. Le 1-phénazinecarboxamide provoque des altérations morphologiques sur les hyphes d' Arthroderma simii. À forte concentration (200 pg/ml), il n'est pas toxique pour les leucocytes de rat

Evolution des teneurs en polyamines dans les boutons floraux, les fleurs et les jeunes baies de Vitis vinifera L. (cv. Cabernet Sauvignon) atteints d’eutypiose

Development of polyamine levels in flower buds, flowers and young berries of Vitis vinifera L. (cv. Cabernet Sauvignon) infected by eutypiosis fungus, Eutypa lata Effects of eutypiosis on polyamines (PAs) and tyramine contents in flower buds, flowers and young berries of grapevine were studied to determine the relationship between these components and disease development. The study was performed using organs (1) from healthy vines, (2) healthy appearing organs from vines with one symptomless arm and one diseased arm, and (3) diseased organs. Free PAs, conjugated polyamines ( PAs-fpm) and wall-bound polyamines (PAs-mm) were analysed in Vitis vinifera L. cv. Cabernet Sauvignon which is sensitive to eutypiosis. An increase of PAs-mm and a decrease of free PAs were observed in diseased organs; particularly putrescine and diaminopropane were concerned. On the other hand these substances were not affected by eutypiosis in the healthy appearing organs. Alterations of free PAs and PAs-mm in diseased organs were correlated with alteration of the flowering process. In contrast, the PAsfpm levels decreased in diseased organs in response to eutypiosis whereas they increased in healthy appearing organs. This group of PAs seems to play an important role in the response to Eutypa lata attacks to prevent the expression of symptoms. Accumulation of the conjugated tyramine in diseased and healthy appearing organs as a response to eutypiosis might be used as marker of Eutypa lata infection

Detection of Diarrheic Shellfish Poisoning and Azaspiracid Toxins in Moroccan Mussels: Comparison of the LC-MS Method with the Commercial Immunoassay Kit

Diarrheic shellfish poisoning (DSP) is a recurrent gastrointestinal illness in Morocco, resulting from consumption of contaminated shellfish. In order to develop a rapid and reliable technique for toxins detection, we have compared the results obtained by a commercial immunoassay-“DSP-Check” kit” with those obtained by LC-MS. Both techniques are capable of detecting the toxins in the whole flesh extract which was subjected to prior alkaline hydrolysis in order to detect simultaneously the esterified and non esterified toxin forms. The LC-MS method was found to be able to detect a high level of okadaic acid (OA), low level of dinophysistoxin-2 (DTX2), and surprisingly, traces of azaspiracids 2 (AZA2) in mussels. This is the first report of a survey carried out for azaspiracid (AZP) contamination of shellfish harvested in the coastal areas of Morocco. The “DSP-Check” kit was found to detect quantitatively DSP toxins in all contaminated samples containing only OA, provided that the parent toxins were within the range of detection and was not in an ester form. A good correlation was observed between the two methods when appropriate dilutions were performed. The immunoassay kit appeared to be more sensitive, specific and faster than LC-MS for determination of DSP in total shellfish extract

Mise en oeuvre de deshydrogenases dans un reacteur electrochimique de regeneration du NAD"+

SIGLEINIST T 71636 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Antimicrobial Activity of Untenospongin B, a Metabolite from the Marine Sponge Hippospongia communis collected from the Atlantic Coast of Morocco

(-)-Untenospongin B isolated from the marine sponge Hippospongia communis has been tested for its antimicrobial activity against bacteria and human pathogenic fungi using agar disk method and was found to possess a broad and strong activity toward the test organisms. Its antifungal activity was further characterized by determination of the minimum inhibitory concentration (MIC) against five fungal species using broth microdilution method

Proceedings of the 3rd International Conference of Economics and Management (CIREG 2016) Volume I

This book brings together papers presented at the 3rd Conference of Research in Economics and Management (CIREG) held in Morocco in May 2016. With a focus on the challenges of SMEs and innovative solutions, they highlight the contribution of researchers in the fields of business and management, with all their micro and macro-economic aspects. They shed light on the universal scientific vision of the importance of SMEs with answers relevant to their local context and adapted to their specific national situation. The relevance of SME research lies in its heuristic value of analyzing change, rather than in constructing a category, a particularly useful empirical concept. This first volume is focused on economic issues

Proceedings of the 3rd International Conference of Economics and Management (CIREG 2016) Volume I

This book brings together papers presented at the 3rd Conference of Research in Economics and Management (CIREG) held in Morocco in May 2016. With a focus on the challenges of SMEs and innovative solutions, they highlight the contribution of researchers in the fields of business and management, with all their micro and macro-economic aspects. They shed light on the universal scientific vision of the importance of SMEs with answers relevant to their local context and adapted to their specific national situation. The relevance of SME research lies in its heuristic value of analyzing change, rather than in constructing a category, a particularly useful empirical concept. This first volume is focused on economic issues