Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics.

Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner

Hemangiopericytoma of the neck

Hemangiopericytoma (HPC) is an exceedingly rare tumor of uncertain malignant potential. Approximately 300 cases of HPC have been reported since Stout and Murray described HPCs as "vascular tumors arising from Zimmerman's pericytes" in 1942. After further characterization, the WHO reclassified HPC as a fibroblastic/myofibroblastic tumor. Long term follow up is mandatory because the histologic criteria for prediction of biologic behavior are imprecise. There are reports of recurrence and metastasis many years after radical resection. The head and neck incidence is less than 20%, mostly in adults

Studies in Biochemical Pathology in Trichinosis. II. Changes in Liver and Muscle Glycogen and Some Blood Chemical Parameters in Mice

Paper by George L. Stewar

Selenium in serum and neoplastic tissue in breast cancer: correlation with CEA

Trace element selenium (Se) is regarded to be a breast cancer preventive factor involved in multiple protective pathways. In all, 80 women with breast cancer who underwent a radical mastectomy were enrolled in the study. Serum Se and carcinoembryonic antigen levels were measured using a fluorometric and IRMA assay, respectively. Se tissue concentration was determined by a tissue extracting fluorometric assay. For statistical analysis purposes t-test was used and P-values <0.001 were regarded as statistically significant. Serum Se was 42.5±7.5 μg l−1 in breast cancer patients and 67.6±5.36 μg l−1 in the age-matched control group of healthy individuals. Serum carcinoembryonic antigen in patients was 10±1.7 U ml−1 (normal <2.5 U ml−1 in nonsmokers/<3.5 U ml−1 in smokers). A statistically significant difference was found for both serum Se and CEA between two groups studied (P<0.001). Neoplastic tissue Se concentration was 2660±210 mg g−1 tissue; its concentration in the adjacent non-neoplastic tissue was 680±110 mg g−1 tissue (P<0.001). An inverse relationship between Se and CEA serum levels was found in the two groups studied (r=−0.794). There was no correlation between serum/tissue Se concentration and stage of the disease. The decrease in serum Se concentration as well as its increased concentration in the neoplastic breast tissue is of great significance. These alterations may reflect part of the defence mechanisms against the carcinogenetic process

Exfoliativ-zytologische Untersuchungen des Epithels der Mundhöhle mit besonderer Berücksichtigung auf die Gingiva

Da bismo što bolje odredili stupanj maturacije površinskog epitela pojedinih regija sluznice usne šupljine pa tako i gingive, služimo se eksfolijativnom citologijom. Tehnika površinskog razmaza vrlo je podesna, jer polikromatskim bojadisanjem celularnih detalja omogućavamo morfološko diferenciranje i određivanje zrelosti svake stanice posebno. Podaci iz Literature o diferenciranju endocelularne morfološke strukture pojedinih regija epitela zdrave i patološki promijenjene gingive često su oprečni. Vjerojatno je tomu uzrok utjecaj različitih funkcijskih podražaja i nedovoljno poznavanje faktora koji dovode do promjene u epitelu gingive i tako stvaraju podlogu za patološke procese u parodontu. Eksfolijativno citološka ispitivanja površinskog epitela zdrave i patološki promijenjene gingive, prikazana u ovom radu, prilog su uočavanju povezanosti endocelularne strukture epitela gingive i funkcijskih manifestacija parodoncija.In order to determine in the best possible way the degree of maturation of the superficial epithelium in individual regions of the oral mucosa and thus of the gingiva, exfoliative cytology is often used. The technique of the superficial smear is very suitable because the polychromatic staining of the cellular details enables us to make the morphological differentiation and determination of the maturity of each cell separately. Data from the literature on the differentiation of the endocellular morphological structure of individual epithelial regions in a healthy and in a pathologically alterated gingiva are often contradictory. Possibly this may be ascribed to the effect of different functional stimuli and insufficient knowledge of the factors leading to a change in the gingival epithelium, thus forming the basis for pathological processes in the parodontium. Exfolliative cytology of the superficial epithelium of a healthy and a pathologically changed gingiva reported on in this paper is a contribution towards better knowledge of the link between the endocellular structure of the gingival epithelium and functional manifestations in the parodontium.Um den Reifegrad des Oberflächenepithels von einzelnen Regionen der Mundhöhlenschleimhaut zu bestimmen, bedienten wir uns der exfoliativ-zytologischen Methode. Die Technik des oberflächlichen Ausstrichs ist sehr geeignet, da die polychromatische Färbung der Zelibestandteile die morphologische Differenzierung und Reifebestimmung jeder einzelnen Zelle ermöglicht. Die Literaturangaben über die Differenzierung der endozellulären morphologischen Struktur der einzelnen Epithelregionen der gesunden und kranken Gingiva, sind häufig kontradiktorisch. Wahrscheinlich wird dies durch den Einfluss verschiedener Funktionsreize und infolge ungenügender Kenntnis der Faktoren welche zu Veränderungen im Epithel der Gingiva führen und derart die Grundlagen zu pathologischen Prozessen im Parodont schaffen, verursacht. Die angeführten exfoliativ-zytologischen Untersuchungen stellen einen Beitrag zur Kenntnis der Interaktion zwischen endozellulärer Eplithelstruktur der Gingiva und der Funktion des Parodonts, dar

Mammary-carcinoma cells in mouse liver: infiltration of liver tissue and interaction with Kupffer cells.

Interactions between TA3 mammary-carcinoma cells and liver cells were studied with the electron microscope in mouse livers that had been perfused with a defined medium containing the tumour cells. Infiltration of liver tissue by the TA3 cells proceeded in the following steps. First, numerous small protrusions were extended through endothelial cells and into hepatocytes. Next, some cells had larger processes deeply indenting hepatocytes. Finally a few tumour cells became located outside the blood vessels. Two variant cell lines, TA3/Ha and TA3/St, differing in cell coat and surface charge, did not differ in the extent of infiltration. TA3/Ha cells were often encircled by thin processes of liver macrophages (Kupffer cells). Encircled cells were initially intact, but later some of them degenerated. These observations suggest that TA3/Ha cells were phagocytized by the Kupffer cells. Encirclement appeared to be inhibited after only 30 min, when many cells were still partly surrounded. Encirclement of TA3/St was much less frequent. After injection of tumour cells intra-portally in vivo, similar results were obtained, which demonstrated the validity of the perfused liver model. TA3/Ha cells formed much fewer tumour nodules in the liver than TA3/St cells

Gingival Changes Following Scaling, Root Planing and Oral Hygiene—A Biometric Evaluation

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141193/1/jper0245.pd

Antiphospholipid Antibodies in Patients with Myasthenia Gravis

We measured antiphospholipid antibodies in sera from 94 patients with myasthenia gravis (MG). We found lgG aCL in 14/94 (14.9 % )lgM aCL in 6/94 (6.4 %) and LA in 4/56 (7.1 %) patients with MG. As a whole 21 of 94 (22.3 % ) patients with MG had some aPL. There was no correlation between the presence of aPL and the severity of MGthe presence of hyperplasia of thymustiter of the antiacetylcholine receptor antibodies or anti-single stranded DNA antibodies. Though the percentage of malignant thymoma with aPL were higher than that of malignant thymoma without aPLwe thought that aPL were not the specific antibody in malignant thymoma. In MGaPL did not play as the aCL syndrome and seemed to be non-specific antibodies

A study of chemical and cellular changes induced by Trichinella infections

The progressive host responses to the invading larvae of Trichinella spiralis and Trichinella pseudospiralis were monitored in the peritoneal cavity and skeletal muscles of mice;Activated peritoneal macrophages, obtained 15 days after oral infection of mice with T. spiralis, were found to secrete a transiently appearing protein (TAP). This protein has been purified by the use of polyacrylamide gel electrophoresis and isoelectrofocusing techniques. Chemical analysis revealed that TAP is acidic due to the high content of glutamic and aspartic acids. With 50% of its total amino acids being non-polar, this 50,000 dalton molecule tends to crystallize at its isoelectric point (5.7) and in deionized water. High-titer (1.5 x 10(\u27-5)) specific antibodies (IgG) to TAP were used to demonstrate that peritoneal macrophages are solely responsible for TAP production;The biological function of TAP is not known. It is not a plasma protein, a constituent substance in the cytoplasm of resident white blood cells, a membrane-bound molecule, an elastase or a plasminogen activator. However, TAP may have an immuno-regulatory function;T. spiralis and T. pseudospiralis infections of murine skeletal muscles were investigated using synchronous infections for histological studies and asynchronous infections for ultrastructural studies. The penetration of muscle cells by either species induces marked morphological changes in the host cells including: (1) an increase in the amount of endoplasmic reticulum, ribosomes, mitochondria and Golgi apparatus; (2) an increase in the size of muscle cell nuclei with enlarged nucleoli; and (3) degeneration and loss of myofilaments;In comparision with T. spiralis infected muscle cells, cells infected with T. pseudospiralis show a slower rate of myofilament degeneration and lighter basophilic staining and remain elongated and non-encapsulated;Tissue autoradiography plus various superimposed and concomitant infections with both species indicate that T. spiralis-infected muscle cells are intrinsically responsible for capsule formation. Encapsulation is independent of host fibroblasts and the presence of T. pseudospiralis. The lower content of guanosine and cytidine-rich RNA in the cytoplasm of T. pseudospiralis infected muscle cells suggests that collagen mRNA may not be present for the encapsulation processes

Comprehensive lung injury pathology induced by mTOR inhibitors

Molecular Targets in Oncology[Abstract] Interstitial lung disease is a rare side effect of temsirolimus treatment in renal cancer patients. Pulmonary fibrosis is characterised by the accumulation of extracellular matrix collagen, fibroblast proliferation and migration, and loss of alveolar gas exchange units. Previous studies of pulmonary fibrosis have mainly focused on the fibro-proliferative process in the lungs. However, the molecular mechanism by which sirolimus promotes lung fibrosis remains elusive. Here, we propose an overall cascade hypothesis of interstitial lung diseases that represents a common, partly underlying synergism among them as well as the lung pathogenesis side effects of mammalian target of rapamycin inhibitors