Experimental and analytical determination of characteristics affecting light aircraft landing-gear dynamics

An experimental and analytical investigation was conducted to determine which characteristics of a light aircraft landing gear influence gear dynamic behavior significantly. The investigation focused particularly on possible modification for load control. Pseudostatic tests were conducted to determine the gear fore-and-aft spring constant, axial friction as a function of drag load, brake pressure-torque characteristics, and tire force-deflection characteristics. To study dynamic tire response, vertical drops were conducted at impact velocities of 1.2, 1.5, and 1.8 m/s onto a level surface; to determine axial-friction effects, a second series of vertical drops were made at 1.5 m/s onto surfaces inclined 5 deg and 10 deg to the horizontal. An average dynamic axial-friction coefficient of 0.15 was obtained by comparing analytical data with inclined surface drop test data. Dynamic strut bending and associated axial friction were found to be severe for the drop tests on the 10 deg surface

Results from tests of three prototype general aviation seats

Three types of energy absorbing general aviation seats were dynamically tested and evaluated for crash load attenuation. On the basis of the static and dynamic test results, it was recommended that the tubular frame seats be redesigned to initiate stroking at approximately 12 G's rather than the 20 to 25 G range. Lower density foam was recommended for the foam wedge passenger seat

Experimental investigation of dam-break usinglow cost laser sourcesand image processing techniques

Dambreak waves in a horizontal rectangular and smooth channel are measured using a number of low cost laser sources, fluorescent dies and Planar Laser Induced Fluorescence (PLIF). Even in such a simple configuration, the experiment are useful to investigate a number of factors such as: the initial wave formation process, the positive front propagation and the local velocity of the wave. The positive wave fronts and the shape of the waves are compared with analytically determined solution proposed by Ritter. The effect of the relative reservoir high on the wave maximum and shape is shown to reasonably agree with the Ritter solution. The results so obtained are discussed and allow a deeper insight into the mechanics of dam-break waves. The results are readily available for applications, and a significant modification regarding the initial flow conditions is described

The Linear Behaviour of Pathogen Strain of Bacillus anthracis A0843 in Anthrax Subcutaneous Challenge on Rabbit Model

Background: The pathogen strain of Bacillus anthracis A0843, isolated during an anthrax outbreak occurred in Italy, belongs to the Cluster A1a genotype 3. The authors show its activity underlining that the regular behaviour could make it useful as a reference strain for subcutaneous challenge in rabbit model for anthrax vaccines efficacy test. Italy doesn't use Ames strain because the restrictive measures, imposed after the bioterroristic events occurred in October 2001 in USA, reduced the movements of pathogen agents between reference laboratories in the world. It is necessary to adopt new rules that favour the security and the regularity of the research. Method: This study was done, during 3 years, on 50 New Zeeland rabbits, males and females, with a weigh between 1.200 and 1500 grams. The site of injection was back in the space between the two scapulae. It was used 20 LD50 (about 40.000 spores) of the pathogen strain according to the European Pharmacopoeia. Results: It was observed that anthrax begins to kills after 48 hours from the infection. At 72 hours the percentage of survival is 56,66%; at 96 hours is 30%. It was observed that two animals that survived after 120 hours from infection didn't die. Conclusion: The LD50 of B. anthracis strain A0843 in rabbit is 2.000 spores, less virulent then Ames strain which is characterized of a LD50 of about 1.200 spores. The standard amount of 20 DL50 (about 40.000 spores) of B. anthracis strain A0843 injected in subcutaneous area in rabbits shows a linear behaviour. The higher mortality is observed between 72 and 96 hours. All the animals died within 120 hours from the infection. None of the infected animals survived over this time and we consider it the survival line of anthrax subcutaneous challenge in rabbit. Technical support: Angela Aceti and Nicola Nigro Founds: Ricerca Corrente 2005 of Ministry of Health of Italy This research was done in according to the Decreto legislativo n.116/92 on animal welfar

Material Model Evaluation of a Composite Honeycomb Energy Absorber

A study was conducted to evaluate four different material models in predicting the dynamic crushing response of solid-element-based models of a composite honeycomb energy absorber, designated the Deployable Energy Absorber (DEA). Dynamic crush tests of three DEA components were simulated using the nonlinear, explicit transient dynamic code, LS-DYNA . In addition, a full-scale crash test of an MD-500 helicopter, retrofitted with DEA blocks, was simulated. The four material models used to represent the DEA included: *MAT_CRUSHABLE_FOAM (Mat 63), *MAT_HONEYCOMB (Mat 26), *MAT_SIMPLIFIED_RUBBER/FOAM (Mat 181), and *MAT_TRANSVERSELY_ANISOTROPIC_CRUSHABLE_FOAM (Mat 142). Test-analysis calibration metrics included simple percentage error comparisons of initial peak acceleration, sustained crush stress, and peak compaction acceleration of the DEA components. In addition, the Roadside Safety Verification and Validation Program (RSVVP) was used to assess similarities and differences between the experimental and analytical curves for the full-scale crash test

Simulating the Response of a Composite Honeycomb Energy Absorber

NASA has sponsored research to evaluate an externally deployable composite honeycomb designed to attenuate loads in the event of a helicopter crash. The concept, designated the Deployable Energy Absorber (DEA), is an expandable Kevlar(Registered TradeMark) honeycomb. The DEA has a flexible hinge that allows the honeycomb to be stowed collapsed until needed during an emergency. Evaluation of the DEA began with material characterization of the Kevlar(Registered TradeMark)-129 fabric/epoxy, and ended with a full-scale crash test of a retrofitted MD-500 helicopter. During each evaluation phase, finite element models of the test articles were developed and simulations were performed using the dynamic finite element code, LS-DYNA(Registered TradeMark). The paper will focus on simulations of two full-scale impact tests involving the DEA, a mass-simulator and a full-scale crash of an instrumented MD-500 helicopter. Isotropic (MAT24) and composite (MAT58) material models, which were assigned to DEA shell elements, were compared. Based on simulations results, the MAT58 model showed better agreement with test

Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis

Anthrax is a zoonotic infection caused by the bacterium Bacillus anthracis (BA). Specific identification of this pathogen often relies on targeting genes located on two extrachromosomal plasmids, which represent the major pathogenicity factors of BA. However, more recent findings show that these plasmids have also been found in other closely related Bacillus species. In this study, we investigated the possibility of identifying species-specific and universally applicable marker peptides for BA. For this purpose, we applied a high-resolution mass spectrometry-based approach for 42 BA isolates. Along with the genomic sequencing data and by developing a bioinformatics data evaluation pipeline, which uses a database containing most of the publicly available protein sequences worldwide (UniParc), we were able to identify eleven universal marker peptides unique to BA. These markers are located on the chromosome and therefore, might overcome known problems, such as observable loss of plasmids in environmental species, plasmid loss during cultivation in the lab, and the fact that the virulence plasmids are not necessarily a unique feature of BA. The identified chromosomally encoded markers in this study could extend the small panel of already existing chromosomal targets and along with targets for the virulence plasmids, may pave the way to an even more reliable identification of BA using genomics- as well as proteomics-based techniques

Comparing computer-generated and pathologist-generated tumour segmentations for immunohistochemical scoring of breast tissue microarrays

BACKGROUND: Tissue microarrays (TMAs) have become a valuable resource for biomarker expression in translational research. Immunohistochemical (IHC) assessment of TMAs is the principal method for analysing large numbers of patient samples, but manual IHC assessment of TMAs remains a challenging and laborious task. With advances in image analysis, computer-generated analyses of TMAs have the potential to lessen the burden of expert pathologist review. METHODS: In current commercial software computerised oestrogen receptor (ER) scoring relies on tumour localisation in the form of hand-drawn annotations. In this study, tumour localisation for ER scoring was evaluated comparing computer-generated segmentation masks with those of two specialist breast pathologists. Automatically and manually obtained segmentation masks were used to obtain IHC scores for thirty-two ER-stained invasive breast cancer TMA samples using FDA-approved IHC scoring software. RESULTS: Although pixel-level comparisons showed lower agreement between automated and manual segmentation masks (κ=0.81) than between pathologists' masks (κ=0.91), this had little impact on computed IHC scores (Allred; [Image: see text]=0.91, Quickscore; [Image: see text]=0.92). CONCLUSIONS: The proposed automated system provides consistent measurements thus ensuring standardisation, and shows promise for increasing IHC analysis of nuclear staining in TMAs from large clinical trials

The Development of Two Composite Energy Absorbers for Use in a Transport Rotorcraft Airframe Crash Testbed (TRACT 2) Full-Scale Crash Test

Two composite energy absorbers were developed and evaluated at NASA Langley Research Center through multi-level testing and simulation performed under the Transport Rotorcraft Airframe Crash Testbed (TRACT) research program. A conical-shaped energy absorber, designated the conusoid, was evaluated that consisted of four layers of hybrid carbon-Kevlar plain weave fabric oriented at [+45deg/-45deg/-45deg/+45deg] with respect to the vertical direction. A sinusoidal-shaped energy absorber, designated the sinusoid, was developed that consisted of hybrid carbon-Kevlar plain weave fabric face sheets, two layers for each face sheet oriented at +/-45deg with respect to the vertical direction, and a closed-cell ELFOAM P200 polyisocyanurate (2.0-lb/cu ft) foam core. The design goal for the energy absorbers was to achieve average floor-level accelerations of between 25- and 40-g during the full-scale crash test of a retrofitted CH-46E helicopter airframe, designated TRACT 2. Variations in both designs were assessed through dynamic crush testing of component specimens. Once the designs were finalized, subfloor beams of each configuration were fabricated and retrofitted into a barrel section of a CH-46E helicopter. A vertical drop test of the barrel section was conducted onto concrete to evaluate the performance of the energy absorbers prior to retrofit into TRACT 2. The retrofitted airframe was crash tested under combined forward and vertical velocity conditions onto soft soil. Finite element models were developed of all test articles and simulations were performed using LS-DYNA, a commercial nonlinear explicit transient dynamic finite element code. Test-analysis results are presented for each energy absorber as comparisons of time-history responses, as well as predicted and experimental structural deformations and progressive damage under impact loading for each evaluation level