Mastitis pathogens and their resistance against antimicrobial agents in herds of dairy cows situated in marginal parts of Slovakia

Marginal regions are relatively large part of the area Slovakia which in terms of the economy breeding ruminants can efficiently produce animal commodities only occasionally. Geographic, social and economic stability of these regions is strongly influenced by breeding of ruminants with market milk production. Mastitis is a disease complex that assumes highest clinical and economic significance in milk animals particularly medium to high yielding dairy cattle, usually in and around periparturient period. The objective of this study was to evaluate the effectiveness of different antibiotics against mastitis causing microorganisms during first month of lactation in two herds of 230 and 310 dairy cows situated in marginal parts of Slovakia. Milk samples from quarters were cultured and identified bacteria were subjected to antimicrobial susceptibility test by disc diffusion method to a large number of antibiotics. The prevalence of mastitis in the monitored herds of dairy cows was 26.1% to 17.6%, respectively. A total of 1663 milk samples from udder quarters were investigated, 446 (21.3%) samples were positive. No pathogens were isolated from 1663 (78.4%) milk samples. From all tested bacteria Staphylococcus spp. and Streptococcus spp. which were isolated from subclinical and clinical mastitis, were found amoxicillin + clavulanat and tetradelta to be most effective drug followed by ceftiofur and rifaximin. The significant difference was confirmed between the Staph. aureus and coagulase-negative staphylococci (CoNS) isolates with respect to their susceptibility to the various antibiotics. Antibiotic susceptibility tests should be done to determine the effectiveness of drug that can be used for successful treatment of diseases. Proper isolation and identification of the causative organism play significant role in prevention and control of the diseases

Influence of selenium and vitamin e supplementation during pregnancy on udder health and milk quality in dairy cows at parturition

Selenium and vitamin E ranks among very important antioxidant agents protecting the organism from the effect of reactive oxygen forms. The deficiency of both nutrients during pregnancy in cows often result in metabolic disorders and increased of cases of related diseases (mastitis, retained placenta and other reproductive disorders). The aim of the present work was to study the influence of different dose of parenteral administration selenium and vitamin E in dairy cows prior to parturition on selected metabolic parameters, udder health and milk quality. A total in herd of 270 Holstein cattle in east of Slovakia in a two-four lactation-gestation cycle the control group (C) and 2 experimental groups (D, D1) were selected. All groups were similarly housed, managed and fed with the diet containing from 36 to 42 mg vitamin E and 0.2 mg.kg-1 Se of DM through the study period. In group D a products containing vitamin E and selenium were administered IM four weeks prior to the expected date of parturition in total dose of 1000 mg of dl-α-tocopherol acetate and of 44 mg sodium selenite per cow, respectively. In group D1 the same products were administered twice, four and two weeks prior to parturition. Blood samples were 4 weeks prior to predicted calving date (the time of treatment), on parturition day and at 14th day after calving for assessment of plasma vitamin E and selenium concentrations. Blood samples of the calves were drawn from jugular vein at birth and first colostrum was also collected. The occurrence of the mastitis and retained placenta during the first 14th day after calving were evaluated in all groups. Higher plasmatic and colostral concentrations of selenium and vitamin E were found only in group with repeat application of Se and vitamin E (D1) collected on the day of parturition. At the 14th day of postpartal period a trend of lower occurrence of mastitis was observed in group D1 compared to D group, administered IM once and control group. Parenteral supplementation of selenium and vitamin E during pregnancy had no impact on their transmission into the milk and on the presence of bacterial agents in raw milk obtained from dairy cows diagnosed with mastitis.&nbsp

Effect of peroral supplementation with selenium and vitamin e during late pregnancy on udder health and milk quality in dairy cows

The aim of the experiment was to study selenium and vitamin E sources in the diet of dairy cows in late phase of pregnancy and their effects on udder health and milk quality during the first two weeks after calving. The experiment included 48 cows of Holstein breed divided into four equal groups (n = 12). The first experimental group (D1) was fed with addition of vitamin E in total dose of 1020 dl-a-tocopherol acetate Se/cow per day. The second group (D2) was added the selenium at a dose of 0.3 mg.kg-1 of DM in form of sodium selenite. The third group (D3) was supplemented with addition of vitamin E in combination with sodium selenite in total dose of 1020 dl-a-tocopherol acetate Se/cow per day and of 5.0 mg Se/cow per day, respectively. The control group (C) was without the addition of selenium and vitamin E. In group (D2) with addition of selenium at a dose of 0.3 mg.kg-1 of DM and vitamin E a dose of 50 mg dl-a-tocopherol acetate/kg of DM in diet, increased the plasmatic concentration of selenium and vitamin E and reduced the incidence of mastitis by 13.3% and number of somatic cells during peripartal period in comparison with other groups

Assessment of lipid peroxidation in dairy cows with subclinical and clinical mastitis

Mastitis is still one of the major causes of economic losses in dairy sector. The routine application of bacteriologic examination of milk samples is often insufficient and for this reason, alternative parameters are used to identify trends in the development of the udder health. Therefore, the objectives of this study were to determine the relationship of oxidative product levels, using malondialdehyde (MDA) as a marker on occurrence of mastitis and its causing pathogens. Dairy herd of 223 Slovak spotted cattle were tested for etiology and occurrence of mastitis based on assessment of clinical signs, abnormal udder secretions, Californian Mastitis Test (CMT) with subsequent collecting of milk samples for bacteriological examination. From 892 quarter milk samples were selected for MDA detection 51 subclinical (SM) and 26 clinical mastitis (CM) quarters with positive CMT score and positive bacteriological examination of Staphylococcus spp. and Streptococcus spp. as well 40 healthy quarters. Results showed that among the current pathogens of the mammary gland belong CNS, S. aureus, S. sanguinis, S. uberis and E. coli, which were the most frequently isolated from SM and CM. The highest MDA level was observed from clinical cases of mastitis however, increased MDA levels were detectable from subclinical cases. Bacterial isolates from subclinical quarter milk samples are different levels of MDA. In this study, we found that quarter milk samples infected with S. uberis were higher compared to other pathogens. In conclusion, differences in both severity of mastitis and mastitic pathogens were associated with differences of oxidative products in infected udders

Process Automation in Design Sprint

The topic of the thesis is an automation of the processes that are considered an integral part of the Design sprint methodology. The proposed solution is based on the knowledge gained by participating in one of the design sprints at Kentico Software s.r.o. After analyzing the individual processes, the evaluation of customer feedback on the given prototype was chosen for automation. The created JavaScript solution performs text analysis of the feedback to help speed up its processing

Process Automation in Design Sprint

Táto práca rieši tematiku automatizácie procesov, ktoré sú súčasťou agilnej metodiky Design šprintu. Navrhnuté riešenie vychádza z poznatkov nadobudnutých absolvovaním Design šprintu v spoločnosti Kentico Software s.r.o. Na základe analýzy jednotlivých procesov bolo pre automatizáciu vybrané zhodnotenie zákazníckej spätnej väzby na daný prototyp. Vytvorené riešenie v jazyku JavaScript vykoná textovú analýzu spätnej väzby a urýchľuje tak jej spracovanie.The topic of the thesis is an automation of the processes that are considered an integral part of the Design sprint methodology. The proposed solution is based on the knowledge gained by participating in one of the design sprints at Kentico Software s.r.o. After analyzing the individual processes, the evaluation of customer feedback on the given prototype was chosen for automation. The created JavaScript solution performs text analysis of the feedback to help speed up its processing.

Modal analysis - Measurements versus FEM and artificial neural networks simulation

The article deals with the experimental modal analysis of glass laminates plates with different shape and these results are compared with those obtained by applications of the artificial neural networks (ANN) and finite element method (FEM) simulation. We have investigated the dependence of the generated mode frequency as a function of sample thickness as well as the sample shape (rounding) of glass laminate samples. The coincidence of both experimental and simulated results is very good. © 2011 Springer-Verlag

The study of electrical transport in rubber blends filled by single wall carbon nanotubes

The paper deals with the electrical and mechanical properties of rubber blends filled by single wall carbon nanotubes (SWCNT-0,6 weight %). We have investigated the alternating current resistivity (ACR), real and imaginary part of dielectric constant,and loss factor .The addition of (SWCNT) to a rubber blend decreases ACR and increases constants ,and loss factor.Web of Science216

Heavy consumption of garlic (Allium sativum) exert nephro- and pulmonary toxicity at maternal and embryonic level in the Albino rats

ABSTRACTDespite the health benefits of herbals, random misuse of such therapy without consult tends to participate in public health issues. This study describes the nephro-and pulmonary toxicity effects of heavy consumption of garlic (Allium sativum) during developmental period. Two groups of ten healthy pregnant female Albino rats each were formed from the 20 in total. Gavages of distilled water were administered to the control group. Since garlic group rats orally received garlic aqueous extract (GAE) 1 ml/rat daily from gestational day (GD) 6 to 19. Kidney functions and lipid biomarkers were assayed along with histopathological investigations. Our results confirmed that the group that received garlic had significantly increased serum levels of urea, cholesterol, triglycerides and LDL. Moreover, the foetal and maternal tissues of the kidney and lung exhibited a varying degree of histopathological manifestations characterized by kidney necrosis and lung haemorrhage, severe congestion, and thickening of the blood vessels. Renal and pulmonary fibrosis were detectable by Masson’s trichrome. A positive expression of the foetal kidney and placental tissues towards Bax protein was noticed. In conclusion, depending on existing findings, the administration of higher concentrations of garlic could explode renal and pulmonary toxicity and decrease the rat’s weight