263 research outputs found

    The Role of Oxidation Compounds in Biofilm Growth on Polyethylene Geomembrane Barriers Used in Landfill

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    In a model study, polyethylene was preoxidized and incubated for a period of 7 months at 40°C in two different municipal solid waste leachates. During the postexperimental analyses, specific attention was paid to the carbonyl species and carboxylic acid depletion during the environmental exposure because it is well known that carboxylic acids are believed to be a potential substrate for the development of microorganisms. The results showed that the carbonyl as well as the carboxylic acid depletion observed follows first-order kinetics. The biofilm formation was characterized using a suite of analytical techniques, and its formation was compared with the carboxylic acid and carbonyl depletion profil

    Antioxidant and Antimicrobial Properties of the Fir Buds Syrup

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    The fir buds as well as the fir buds extracts (such as fir buds syrup) are used in traditional medicine and phytotheraphy for the alleviation of light to medium forms of respiratory diseases and for the prophylaxis or treatment of some childhood disorders. The antioxidant and antimicrobial properties of the two types fir buds syrups (cold pressed and boiled) taken into study were demonstrated by its content in vitamin C, flavonoids and by the inhibitory effect on E.coli, B. cereus, S.aureus, L. monocytogenes, Salmonella thypi

    An integral equation method for the inverse conductivity problem

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    We present an image reconstruction algorithm for the Inverse Conductivity Problem based on reformulating the problem in terms of integral equations. We use as data the values of injected electric currents and of the corresponding induced boundary potentials, as well as the boundary values of the electrical conductivity. We have used a priori information to find a regularized conductivity distribution by first solving a Fredholm integral equation of the second kind for the Laplacian of the potential, and then by solving a first order partial differential equation for the regularized conductivity itself. Many of the calculations involved in the method can be achieved analytically using the eigenfunctions of an integral operator defined in the paper.Comment: 15 pages, 8 figure

    Development of functional beverage from wheat grass juice

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    The juice from wheat grass is called "green blood" and is an excellent detoxifying, facilitating the elimination of toxins and fats from body. In the form of fresh juice, it has high concentrations of chlorophyll, active enzymes, vitamins and other nutrients. The aim of this work was the development and characterization of a functional beverage from green wheat juice by adding apple and limes. The antioxidant capacity, vitamin C, polyphenols and flavonoids content were quantified by using spectrophotometry. The final product was pasteurized and evaluated by the content of bioactive compounds during storage at intervals of 7 and 14 days. During storage there were found slight decreases of the contents of bioactive compounds. The juice obtained has a sweet-sour taste, a unique flavor and a very pleasant smell. This product targets all categories of consumers and represents an ideal morning snack for those who are concerned about a healthy lifestyle

    Bullying homofóbico no contexto escolar em Portugal

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    O presente estudo pretendeu explorar o fenómeno do bullying homofóbico em Portugal relativamente à sua prevalência, consequências e formas de agressão. Mediante um questionário online, distribuído pelos núcleos locais da Associação rede ex aequo e preenchido por 184 estudantes, verificou­?se que, à semelhança de resultados internacionais, o recinto escolar é o local privilegiado para a ocorrência deste fenómeno. Os resultados indicam que prevalece a violência psicológica e a vitimização de rapazes; os comportamentos de agressão são desvalorizados; subsiste uma não intervenção nas situações presenciadas; existem consequências psicológicas significativas para as vítimas de bullying homofóbico, em comparação com as não­?vítimas. Defende­?se a importância de conceber programas de sensibilização, de criar medidas de protecção para as vítimas, e ainda de aprofundar a investigação desta temática.info:eu-repo/semantics/publishedVersio

    The cohesin ring concatenates sister DNA molecules

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    Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit protein complex called cohesin whose Scc1, Smc1, and Smc3 subunits form a tripartite ring structure. It has been proposed that cohesin holds sister DNAs together by trapping them inside its ring. To test this, we used site-specific cross-linking to create chemical connections at the three interfaces between the ring’s three constituent polypeptides, thereby creating covalently closed cohesin rings. As predicted by the ring entrapment model, this procedure produces dimeric DNA/cohesin structures that are resistant to protein denaturation. We conclude that cohesin rings concatenate individual sister minichromosome DNAs

    Severe Acute Respiratory Syndrome–associated Coronavirus in Lung Tissue

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    Efforts to contain severe acute respiratory syndrome (SARS) have been limited by the lack of a standardized, sensitive, and specific test for SARS-associated coronavirus (CoV). We used a standardized reverse transcription-polymerase chain reaction assay to detect SARS-CoV in lung samples obtained from well-characterized patients who died of SARS and from those who died of other reasons. SARS-CoV was detected in all 22 postmortem lung tissues (to 109 viral copies/g) from 11 patients with probable SARS but was not detected in any of the 23 lung control samples (sample analysis was blinded). The sensitivity and specificity (95% confidence interval) were 100% (84.6% to 100%) and 100% (85.1% to 100%), respectively. Viral loads were significantly associated with a shorter course of illness but not with the use of ribavirin or steroids. CoV was consistently identified in the lungs of all patients who died of SARS but not in control patients, supporting a primary role for CoV in deaths

    Comparison of different methods for delayed post-mortem diagnosis of falciparum malaria

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    <p>Abstract</p> <p>Background</p> <p>Between 10,000 and 12,000 cases of imported malaria are notified in the European Union each year. Despite an excellent health care system, fatalities do occur. In case of advanced autolysis, the post-mortem diagnostic is impaired. Quicker diagnosis could be achieved by using rapid diagnostic malaria tests.</p> <p>Methods</p> <p>In order to evaluate different methods for the post-mortem diagnosis of <it>Plasmodium falciparum </it>malaria in non-immunes, a study was performed on the basis of forensic autopsies of corpses examined at variable intervals after death in five cases of fatal malaria (with an interval of four hours to five days), and in 20 cases of deaths unrelated to malaria. Detection of parasite DNA by PCR and an immunochromatographic test (ICT) based upon the detection of <it>P. falciparum </it>histidine-rich protein 2 (PfHRP2) were compared with the results of microscopic examination of smears from cadaveric blood, histopathological findings, and autopsy results.</p> <p>Results</p> <p>In all cases of fatal malaria, post-mortem findings were unsuspicious for the final diagnosis, and autoptic investigations, including histopathology, were only performed because of additional information by police officers and neighbours. Macroscopic findings during autopsy were unspecific. Histopathology confirmed sequestration of erythrocytes and pigment in macrophages in most organs in four patients (not evaluable in one patient due to autolysis). Microscopy of cadaveric blood smears revealed remnants of intraerythrocytic parasites, and was compromised or impossible due to autolysis in two cases. PCR and ICT performed with cadaveric blood were positive in all malaria patients and negative in all controls.</p> <p>Conclusion</p> <p>In non-immune fatalities with unclear anamnesis, ICT can be recommended as a sensitive and specific tool for post-mortem malaria diagnosis, which is easier and faster than microscopy, and also applicable when microscopic examination is impossible due to autolysis. PCR is more expensive and time-consuming, but may be used as confirmatory test. In highly endemic areas where asymptomatic parasitaemia is common, confirmation of the diagnosis of malaria as the cause of death has to rely on histopathological findings.</p
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