86 research outputs found

    Morphometric and Phylogenic Analysis of Six Population Indonesian Local Goats

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    The research objectives were to characterize morphometric and genetic distance between populations of Indonesian local goats. The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variable between Benggala goats (n= 96), Marica (n= 60), Jawarandu (n= 94), (Kacang (n= 217), Muara (n= 30) and Samosir (n= 42). Discriminant analysis used to clasify body weight and body measurements. In the analysis of variance showed that body weight and body measurement (body length, height at withers, thorax width, thorax height, hert girth, skull width and height, tail length and width, ear length and width) of Muara goats was higher (P<0.05) compared to the other groups, and the lowest was in Marica goats. The smallest genetic distance was between Marica and Samosir (11.207) and the highest were between Muara and Benggala (255.110). The highest similarity between individual within population was found in Kacang (99.28%) and the lowest in Samosir (82.50%). The canonical analysis showed high correlation on canon circumference, body weight, skull width, skull height, and tail width variables so these six variables can be used as distinguishing variables among population. The result from Mahalonobis distance for phenogram tree and canonical analysis showed that six populations of Indonesian local goats were divided into six breed of goats: the first was Muara, the second was Jawarandu, the third was Kacang, the fourth was Benggala, the fifth was Samosir and the sixth was Marica goats. The diversity of body size and body weight of goats was observed quite large, so the chances of increasing productivity could be made through selection and mating programs

    Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows

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    Pit-1 gene has been identified as the pituitary specific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total  of 320 Indonesian buffaloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH cattle. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buffaloes

    Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

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    Kappa casein (ĸ-cn) gene as one of the four casein genes commonly has two variant alleles. B allele is positively correlated with milk proteins, so potentially be used as MAS to improve milk protein content. This study aimed to identify genetic variation of the ĸ-casein gene in Holstein-Friesian (HF) from several dairy regions in West Java. Blood samples were taken through jugular vein of lactating heifers and cows, from intensive managements under the Local Government Dairy Breeding Station - Cikole (BBPT Cikole-SP) (82 hds), Embryo Transfer Station (BET) (50 hds), and Eco Farm of Animal Science Faculty of Bogor Agricultural University (EcoFarm) (20 hds); as well as from semi-intensive managements of small dairy farmers under the supervision of the North Lembang Cooperative Unit (KPSBU Lembang) in two villages of Cilumber (98 hds) and Pasar Kemis (92 hds).  Blood samples were also taken via cocsigalis vein of AI (active and non active) HF bulls at the two National AI Stations in Lembang (25 hds) and Singosari (32 hds).  Identification of genetic variation was by applying PCR-RFLP method. Three genotypes were identified, namely AA, AB, and BB, resulting two alleles of A and B. Results entirely showed that the frequencies of the BB HF female were very low, with the range of 0%-6% (vs AA genotype= 10%-54% and AB genotype= 46%-85%), despite of the relatively high frequency of the B allele over the A allele (23%-48% vs. 52%-77%). The low frequency of the observed BB females might be due to the limited AI active BB bulls used for services by the two national AI stations (0%-4%)

    Berudu Anura di Sungai Kedurang, Bengkulu Selatan dan Pusat Pendidikan Konservasi Alam Bodogol, Jawa Barat

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    This paper aims is to identify and to describe morphological characteristic of tadpoles in Kedurang River, South Bengkulu and Cisuren River, PPKA Bodogol. Visual Encounter Survey along the river method was used to collect the sample of tadpoles. Three species were found in both rivers, namely Bufo melanostictus, Limnonectes sp. and Rhacophorus sp

    Intermediate Role of the Criterion of Focus on the Students Benefiting in the Relationship between Adopting the Criterion of Partnership and Resources and Achieving Community Satisfaction in the Palestinian Universities

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    The study aimed at identifying the intermediate role of the criterion of emphasis on students and beneficiaries in the relationship between adopting the criterion of partnership and resources and achieving the satisfaction of the society. The study used the analytical descriptive method. The study was conducted on university leadership in Al-Azhar, Islamic and Al-Aqsa Universities. The sample of the study consisted of (200) individuals, 182 of whom responded, and the questionnaire was used in collecting the data. The study reached a number of results, the most important of which were: The level of adoption by the Palestinian universities of the criterion of partnership and resources came to a large extent. The level of adoption by the Palestinian universities of the criterion of emphasis on the beneficiary students was very high. The criterion of partnership and resources to achieve the satisfaction of the community surrounding the Palestinian universities, the existence of a statistically significant impact of the adoption of the criterion of partnership and resources to strengthen the focus on students benefiting in Palestinian universities, The focus on the students benefiting from the satisfaction of the community surrounding the Palestinian universities, the existence of a statistically significant impact on the adoption of the criterion of partnership and resources to achieve the satisfaction of the community surrounding the Palestinian universities, The study presented a number of recommendations, most important of which are: Increase interest in the resource and partnership criterion through enhancing the university's resources and developing internal and external partnerships to serve the university and the society. Increase the focus on students and beneficiaries by providing an appropriate educational environment and academic programs that meet the needs of the labor market. Through the strengthening of existing community service centers and the establishment of new community centers, strengthening the partnership of industrial companies and promoting scientific research for the benefit of universities and society

    The Availability of the Resource Standard and Partnership as One of the Possibilities of Excellence in Palestinian Universities According to the European Model

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    The study aimed to identify the availability of the resource and partnership standard as one of the possibilities of excellence in Palestinian universities according to the European model. The study used the analytical descriptive method. The study was conducted on the university leadership at Al - Azhar and Islamic Universities, where the study population consisted of (282) individuals. The study sample consisted of (135) individuals, (119) of them responded, and the questionnaire was used in collecting the data. The study has reached a number of results, the most important of which is the existence of a high standard of resources and partnership in the Palestinian public universities operating in the southern governorates. The fields of the resource and partnership criteria were as follows: (79.8%) management of internal and external partnerships; management of technical resources (technology) (76.4%), Buildings, equipment and resources (76%), Finance Management (72.8%). The study presented a number of recommendations, the most important of which is: Increasing the interest of universities in applying the criteria of resources and partnership as one of the criteria of excellence, increasing the interest of universities in managing finance and obtaining funding for their various activities, periodic maintenance of buildings and equipment and ensuring their suitability to the requirements of the educational process

    Morphometric and Phylogenic Analysis of Six Population Indonesian Local Goats

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    The research objectives were to characterize morphometric and genetic distance between populations of Indonesian local goats. The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variable between Benggala goats (n= 96), Marica (n= 60), Jawarandu (n= 94), (Kacang (n= 217), Muara (n= 30) and Samosir (n= 42). Discriminant analysis used to clasify body weight and body measurements. In the analysis of variance showed that body weight and body measurement (body length, height at withers, thorax width, thorax height, hert girth, skull width and height, tail length and width, ear length and width) of Muara goats was higher (P<0.05) compared to the other groups, and the lowest was in Marica goats. The smallest genetic distance was between Marica and Samosir (11.207) and the highest were between Muara and Benggala (255.110). The highest similarity between individual within population was found in Kacang (99.28%) and the lowest in Samosir (82.50%). The canonical analysis showed high correlation on canon circumference, body weight, skull width, skull height, and tail width variables so these six variables can be used as distinguishing variables among population. The result from Mahalonobis distance for phenogram tree and canonical analysis showed that six populations of Indonesian local goats were divided into six breed of goats: the first was Muara, the second was Jawarandu, the third was Kacang, the fourth was Benggala, the fifth was Samosir and the sixth was Marica goats. The diversity of body size and body weight of goats was observed quite large, so the chances of increasing productivity could be made through selection and mating programs

    Aplikasi Teknik Environmental Dna(edna) untuk Deteksi Spesies Cheraxquadricarinatus (Von Martens 1868) Menggunakan Sampel Air

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    Cherax quadricarinatus merupakan spesies introduksi yang berasal dari Australia. Siklus hidup yang cepat dan toleransi yang tinggi terhadap lingkungan mengakibatkan C. quadricarinatusmudah menginvasi ekosistem perairan. Penelitian ini bertujuan untuk mengaplikasikan teknik eDNA secara cepat untuk deteksi C.quadricarinatus. Teknik ekstraksi dilakukan dengan menggunakan teknik pengendapan. Validasi keberadaan C.quadricarinatusdilakukan dengan menggunakan metode pengembangan PCR dan desain primer spesifik. Hasil menunjukkan bahwa keberadaan C.quadricarinatusterdeteksi sebanyak 60%dari keseluruhan badan perairan. Kombinasi protokol deteksi eDNA dan metode pengembangan PCR, serta primer spesifik yang didesain dapat digunakan untuk mendeteksi keberadaan C. quadricarinatus
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