437 research outputs found

    The early evolution of land plants, from fossils to genomics: a commentary on Lang (1937) ‘On the plant-remains from the Downtonian of England and Wales'

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    © 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. The file attached is the published version of the article

    Characteristic Vertical Response of a Footbridge Due to Crowd Loading

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    The characteristic vertical vibration of a flexible footbridge subject to crowd loading is examined in this paper. Typically, bridge vibrations produced from a crowd of pedestrians are estimated by using an enhancement factor applied to the effect caused by a single pedestrian. In this paper, a single pedestrian model, represented by a spring mass damper, which incorporates variables such as pedestrian mass and body stiffness, is used to calibrate a computationally efficient moving force model. This calibrated moving force model is further used in Monte Carlo simulations of non-homogenous crowds to estimate characteristic vertical vibration levels. Enhancement factors, which could be applied to simple single pedestrian moving force models in estimating the response due to a crowd are thus derived. Such enhancement factors are then compared to previously published values. It is found that the greatest difference between the spring mass damper and moving force models respectively occurs when the bridge frequency is at the mean crowd pacing frequency. For bridges with frequencies even slightly removed from this mean, moving force models appear adequate

    Multiple-locus variable number of tandem repeat analysis (MLVA) of Irish verocytotoxigenic Escherichia coli O157 from feedlot cattle: uncovering strain dissemination routes

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    <p>Abstract</p> <p>Background</p> <p>The identification of the routes of dissemination of <it>Escherichia coli (E. coli) </it>O157 through a cohort of cattle is a critical step to control this pathogen at farm level. The aim of this study was to identify potential routes of dissemination of <it>E. coli </it>O157 using Multiple-Locus Variable number of tandem repeat Analysis (MLVA).</p> <p>Results</p> <p>Thirty-eight environmental and sixteen cattle faecal isolates, which were detected in four adjacent pens over a four-month period were sub-typed. MLVA could separate these isolates into broadly defined clusters consisting of twelve MLVA types. Strain diversity was observed within pens, individual cattle and the environment.</p> <p>Conclusion</p> <p>Application of MLVA is a broadly useful and convenient tool when applied to uncover the dissemination of <it>E. coli </it>O157 in the environment and in supporting improved on-farm management of this important pathogen. These data identified diverse strain types based on amplification of VNTR markers in each case.</p

    Controlling Blown Pack Spoilage Using Anti-Microbial Packaging

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    peer-reviewedActive (anti-microbial) packaging was prepared using three different formulations; Auranta FV; Inbac-MDA and sodium octanoate at two concentrations (2.5 and 3.5 times their minimum inhibitory concentration (MIC, the lowest concentration that will inhibit the visible growth of the organisms) against Clostridium estertheticum, DSMZ 8809). Inoculated beef samples were packaged using the active packaging and monitored for 100 days storage at 2 °C for blown pack spoilage. The time to the onset of blown pack spoilage was significantly (p < 0.01) increased using Auranta FV and sodium octanoate (caprylic acid sodium salt) at both concentrations. Moreover, sodium octanoate packs had significantly (p < 0.01) delayed blown pack spoilage as compared to Auranta FV. It was therefore concluded that Auranta FV or sodium octanoate, incorporated into the packaging materials used for vacuum packaged beef, would inhibit blown pack spoilage and in the case of the latter, well beyond the 42 days storage period currently required for beef primalsDepartment of Agriculture, Food and the Marin

    Assessing Integrated Pest Management Implementation and Knowledge Gaps in South Dakota

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    A survey of commercial pesticide applicator training participants was carried out during 2015 winter pesticide certification meetings to assess integrated pest management (IPM) knowledge gaps. Overall, the majority of the respondents reported that they have adequate access to IPM information and that they apply IPM principles in their pest management programs. Preventive fungicide use was identified as a regular practice by half the respondents and was dependent on the region of the state. Participants identified basic pest identification as an area in which more resources are needed. Online information and field days were the preferred options for accessing outreach and Extension
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