Effects of Low Frequency Ultrasonic Treatment on the Maturation of Steeped Greengage Wine

To accelerate wine maturation, low frequency ultrasonic waves of 28 kHz and 45 kHz were used to treat the steeped greengage wine. The contents of total acid, total ester, fusel oils and the wine chromaticity were determined before and after the ultrasonic treatment. The volatile compounds were analysed by GC–MS method, and the sensory quality was evaluated by panelist. The results indicated that ultrasonic treatment of the steeped greengage wine at 45 kHz 360Wfor 30 min was effective to accelerate the aging process, where the fusel oils and alcohol compounds were significantly reduced and acid and ester compounds were significantly increased

Numerical Study on Spout Elevation of a Gas-Particle Spout Fluidized Bed in Microwave-Vacuum Dryer

The dynamic characteristics of gas-particle spout fluidized bed in a pulsed spouted microwave-vacuum drying system (PSMVD) were investigated. The spout fluidization process in a pseudo-2-D spout fluidized bed was simulated by computational fluid dynamics (CFD) using the inviscid two-fluid theory method (TFM) based on the kinetic theory of granular flow. The dynamic characteristics of the spout fluidized bed and the effect of spout elevation on the particle movement were revealed, which could be used to improve the uniformity of particle mixing and microwave heating. The mathematical model demonstrated that the spout fluidization process includes isolated, merged and transitional jets and the fluidization at a specific spout gas velocity has a start-up stage and a quasi-steady fluidization stage. The spout velocity was an important factor controlling particle status in the spout fluidized bed and a critical velocity was identified for effect transition of the flow pattern. There was an approximately linear correlation between the jet penetration depth and the spout velocity. When the spout gas velocity increased up to the critical velocity region, the pressure drop tended to convert from negative pressure to positive pressure

Effect of different drying methods on the protein and product quality of hairtail fish meat gel

Three different methods, namely hot air drying (HA), microwave vacuum drying (MV), and vacuum freeze drying (FD), were employed to investigate the effect of drying method on the quality of hairtail fish meat gel. Compared with HA and MV, FD samples showed a better quality in terms of moisture content, water absorption index, and water solubility index, and had the highest overall acceptance in sensory evaluation. FD preserved the protein from degradation and formed an ordered porous microstructure. The nitrogen fraction assay revealed that protein was degraded into 40–100 kDa fragments during drying in HA, which was almost not affected by MV and FD. Overall, FD was the most suitable method for drying of meat gel made from hairtail, followed by MV and HA

Design of dual inhibitors of human TNF-α and IL-6 with potentials for the treatment of rheumatoid arthritis

Purpose: To design dual inhibitors of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) with potentials for the treatment of rheumatoid arthritis (RA). Methods: Tumor necrosis factor α (TNF-α) and IL-6 were investigated as potential drug targets for the treatment of RA. Dual inhibitors targeting both TNF-α and IL-6 were designed simultaneously using molecular docking simulation-based in silico virtual screening technique. National Cancer Institute (NCI) diversity set-II consisting of 1818 diverse ligands were screened against both drug targets in order to identify potential lead molecules on the basis of lowest binding energy. Results: Out of 1818 diverse ligand molecules present in the NCI diversity set-II, five lead molecules were selected based on best binding interactions with both target receptors. The results of toxicity profiling showed that compounds ZINC19701771 and ZINC06576501 lacked major toxicity-associated functional groups linked to mutagenic, tumorigenic, irritant and reproductive effects. However, ZINC03898665 and ZINC05015095 possessed some mutagenic and reproductive effects. Compound ZINC01757986 also showed a high chance of mutagenicity. Conclusion: These results indicate that the two lead molecules (ZINC19701771 and ZINC06576501) that showed reliable physicochemical properties can serve as potential candidates for development of anti-arthritis drug for effective inhibition of human TNF-α and IL-6 receptors

Preparation and In Vitro Release of Drug-Loaded Microparticlesfor Oral Delivery Using Wholegrain Sorghum Kafirin Protein

Kafirin microparticles have been proposed as an oral nutraceutical and drug delivery system. This study investigates microparticles formed with kafirin extracted from white and raw versus cooked red sorghum grains as an oral delivery system. Targeted delivery to the colon would be beneficial for medication such as prednisolone, which is used in the management of inflammatory bowel disease. Therefore, prednisolone was loaded into microparticles of kafirin from the different sources using phase separation. Differences were observed in the protein content, in vitro protein digestibility, and protein electrophoretic profile of the various sources of sorghum grains, kafirin extracts, and kafirin microparticles. For all of the formulations, the majority of the loaded prednisolone was not released in in vitro conditions simulating the upper gastrointestinal tract, indicating that most of the encapsulated drug could reach the target area of the lower gastrointestinal tract. This suggests that these kafirin microparticles may have potential as a colon-targeted nutraceutical and drug delivery system

Effect of different drying processes on the protein degradation and sensory quality of Layú: a Chinese dry-curing grass carp

Five different drying methods, sun drying, intermittent drying, low-temperature drying at 5°C, low-temperature drying at 15°C, and hot-air drying at 45°C, were comparatively evaluated based on physicochemical properties and sensory properties of dry-cured Layú. Sun drying and intermittent drying Layú showed superior sensory qualities compared with other dried samples. Based on the comprehensive comparison of sensory qualities and safety concerns, intermittent drying Layú was more acceptable compared to other dry-cured Layú and thus was recommended for dry-curing fish products as it could shorten the drying time with relative constant drying rate, increased flavor amino acid content, and fewer safety concerns

Fatty acid profile, oxidative stability and toxicological safety of bayberry kernel oil

The fatty acid profile, oxidative stability and toxicological safety of bayberry (Myrica rubra Sieb. et Zucc.) kernel oil (BKO) extracted by supercritical carbon dioxide (SC-CO2) and solvent of diethyl ether were assessed. Fatty acid profile was determined by gas chromatography, oxidative stability by placing the sample of 25 g in a blast oven at 50 ± 1 °C to accelerate oxidation and toxicological safety by bacterial reverse mutation (Ames test) and acute oral toxicity in mice. The results demonstrated that in comparison to lard and rapeseed oil, the peroxide values of BKO were higher but the acid values were similar during the incubation test. The Ames test demonstrated no mutagenicity and no obvious acute toxicity were observed, suggesting that the BKO has potential as a novel edible oil

Characterization of polyphenols in Australian sweet lupin (Lupinus angustifolius) seed coat by HPLC-DAD-ESI-MS/MS

Seeds of the legume lupin (Lupinus spp.) are becoming increasingly important as human food. The seed coat, at ~25% of the whole seed of Lupinus angustifolius (Australian sweet lupin, ASL), is the main by-product of lupin kernel flour production. The primary market for lupin seed coat is low value feed with very limited use in foods. In this study, seed coats of six ASL commercial varieties from two growing sites were sampled for identification and quantification of polyphenols using a high-performance liquid chromatography (HPLC) with diode array detector (DAD) and coupled with a triple quadrupole mass spectrometer which equipped with electrospray ionization source (ESI-MS/MS). Three flavones (apigenin-7-O-β-apiofuranosyl-6,8-di-C-β-glucopyranoside, vicenin 2, and apigenin-7-O-β-glucopyranoside), one isoflavone (genistein) and one dihydroflavonol derivative (aromadendrin-6-C-β-D-glucopyranosyl-7-O-[β-D-apiofuranosyl-(1 → 2)]-O-β-D-glucopyranoside), and several hydroxybenzoic and hydroxycinnamic acid derivatives were identified. Considerable variations in levels of individual polyphenols were found but apigenin-7-O-β-apiofuranosyl-6,8-di-C-β-glucopyranoside was the predominant polyphenol in all samples accounting for 73.08–82.89% of the total free polyphenols. These results suggest that ASL seed coat could be valuable dietary source of polyphenols. © 2018 Elsevier Lt

Novel artificial cell microencapsulation of a complex gliclazide-deoxycholic bile acid formulation: A Characterization Study

Gliclazide (G) is an antidiabetic drug commonly used in type 2 diabetes. It has extrapancreatic hypoglycemic effects, which makes it a good candidate in type 1 diabetes (T1D). In previous studies, we have shown that a gliclazide-bile acid mixture exerted a hypoglycemic effect in a rat model of T1D. We have also shown that a gliclazide-deoxycholic acid (G-DCA) mixture resulted in better G permeation in vivo, but did not produce a hypoglycemic effect. In this study, we aimed to develop a novel microencapsulated formulation of G-DCA with uniform structure, which has the potential to enhance G pharmacokinetic and pharmacodynamic effects in our rat model of T1D. We also aimed to examine the effect that DCA will have when formulated with our new G microcapsules, in terms of morphology, structure, and excipients’ compatibility. Microencapsulation was carried out using the Büchi-based microencapsulating system developed in our laboratory. Using sodium alginate (SA) polymer, both formulations were prepared: G-SA (control) at a ratio of 1:30, and G-DCA-SA (test) at a ratio of 1:3:30. Complete characterization of microcapsules was carried out. The new G-DCA-SA formulation was further optimized by the addition of DCA, exhibiting pseudoplastic-thixotropic rheological characteristics. The size of microcapsules remained similar after DCA addition, and these microcapsules showed no chemical interactions between the excipients. This was supported further by the spectral and microscopy studies, suggesting microcapsule stability. The new microencapsulated formulation has good structural properties and may be useful for the oral delivery of G in T1D

Microencapsulation as a novel delivery method for the potential antidiabetic drug, Probucol

Introduction: In previous studies, we successfully designed complex multicompartmental microcapsules as a platform for the oral targeted delivery of lipophilic drugs in type 2 diabetes (T2D). Probucol (PB) is an antihyperlipidemic and antioxidant drug with the potential to show benefits in T2D. We aimed to create a novel microencapsulated formulation of PB and to examine the shape, size, and chemical, thermal, and rheological properties of these microcapsules in vitro. Method: Microencapsulation was carried out using the Büchi-based microencapsulating system developed in our laboratory. Using the polymer, sodium alginate (SA), empty (control, SA) and loaded (test, PB-SA) microcapsules were prepared at a constant ratio (1:30). Complete characterizations of microcapsules, in terms of morphology, thermal profiles, dispersity, and spectral studies, were carried out in triplicate. Results: PB-SA microcapsules displayed uniform and homogeneous characteristics with an average diameter of 1 mm. The microcapsules exhibited pseudoplastic-thixotropic characteristics and showed no chemical interactions between the ingredients. These data were further supported by differential scanning calorimetric analysis and Fourier transform infrared spectral studies, suggesting microcapsule stability. Conclusion: The new PB-SA microcapsules have good structural properties and may be suitable for the oral delivery of PB in T2D. Further studies are required to examine the clinical efficacy and safety of PB in T2D