111 research outputs found
The study of osteoprotective properties of nanoparticulated forms of resveratrol and losartan
The study demonstrated that endothelial dysfunction of bone microvasculature and deterioration of regional blood flow in bone developed eight weeks after ovariectomy in female white Wistar rats thus raising the risk of generalized osteoporosis. Nanoparticulated forms of losartan and resveratrol possessing endothelioprotective action effectively prevented reduction of regional microcirculation in bone tissue through keeping it at the same level as intact rats ha
Study of the microcirculation level in bone with osteoporosis and osteoporotic fractures during therapy with recombinant erythropoietin, rosuvastatin and their combinations
The experiment was carried out in female white Wistar rats. The effects of recombinant erythropoietin, rosuvastatin and their combination were investigated based on the blood supply to the bone after a modelled experimental osteoporosis. It was found that the studied drugs prevent decrease of bone microcirculation in cases of osteoporosis and in callus tissue in experimental osteoporotic fractures, positively influencing the course of reparative regeneration of bone tissu
Test-system for estimation of activity of GSK-3 inhibitors as antihypoxants and differentiation of endothelial progenitors in vitro
A model test-system was developed for validation in vitro of antihypoxic and endothelio-differentiating activity of newly created glycogen synthase kinase-3 inhibitors in comparison with the reference drug β4-thymosi
Study of the influence of L-norvaline, rosuvastatin and their combination on the level of microcirculation in bone tissue in experimental osteoporosis and fractures on its background
The experiment was carried out in female white Wistar rats to investigate the effect of L-norvaline, rosuvastatin and their combination on parameters of blood supply to the bone tissue on models of experimental osteoporosis and osteoporotic fracture
STUDY OF ANTI-OSTEOPOROTIC ACTION OF DRUGS FROM THE GROUP OF STATINS
In an experiment on female Wistar white rats, the osteoprotective effect of atorvastatin, simvastatin and rosuvastatin was studied in a model of experimental osteoporosis caused by bilateral ovariectomy. It was found that after ovariectomy in female rats develops endothelial dysfunction, including the vessels of the microcirculatory of the bone, leading to a deterioration of blood supply to bone tissue and the occurrence of osteoporosis. It was found that atorvastatin, simvastatin and rosuvastatin, possessing endothelioprotective activity, prevent deterioration of blood supply to bone tissue and thinning of bone trabeculae, thus having anti-osteoporotic activity. Key words: osteoporosis, endothelial dysfunction, statins, atorvastatin, simvastatin, rosuvastatin, strontium ranelate
A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity
In this study, we show that the role of nonmuscle myosin
II (NMII)-B in front–back migratory cell polarity is
controlled by a short stretch of amino acids containing
five serines (1935–1941). This motif resides near the
junction between the C terminus helical and nonhelical
tail domains. Removal of this motif inhibited NMII-B assembly,
whereas its insertion into NMII-A endowed an
NMII-B–like ability to generate large actomyosin bundles
that determine the rear of the cell. Phosphomimetic
mutation of the five serines also inhibited NMII-B assembly,
rendering it unable to support front–back polarization.
Mass spectrometric analysis showed that several of
these serines are phosphorylated in live cells. Single-site
mutagenesis showed that serine 1935 is a major regulatory
site of NMII-B function. These data reveal a novel regulatory
mechanism of NMII in polarized migrating cells
by identifying a key molecular determinant that confers
NMII isoform functional specificityThis work is supported by grants SAF2011-24953 from MINECO, FP7
Marie Curie CIG-293719 from the EU, CIVP16A1831 from the Ramon
Areces Foundation (M. Vicente-Manzanares), GM 23244 (A.R. Horwitz),
GM037537 (D.F. Hunt), and the Cell Migration Consortium U54 GM64346
(A.R. Horwitz and D.F. Hunt). M. Vicente-Manzanares is an investigator from
the Ramón y Cajal Program (RYC-2010-06094)
The research of abnormal toxicity and local irritant effect in the Draize test of the drug furacilin, concentrate for the preparation of a solution for local and externaluse
The research of abnormal toxicity and locally irritating effect of the drug Furacilin, concentrate for the preparation of a solution for local and external use in comparison with the drug Furacilin, tablets for the preparation of the solution, was carried ou
MHC-IIB Filament Assembly and Cellular Localization Are Governed by the Rod Net Charge
Actin-dependent myosin II molecular motors form an integral part of the cell cytoskeleton. Myosin II molecules contain a long coiled-coil rod that mediates filament assembly required for myosin II to exert its full activity. The exact mechanisms orchestrating filament assembly are not fully understood., negatively-charged regions of the coiled-coil were found to play an important role by controlling the intracellular localization of native MHC-IIB. The entire positively-charged region is also important for intracellular localization of native MHC-IIB.A correct distribution of positive and negative charges along myosin II rod is a necessary component in proper filament assembly and intracellular localization of MHC-IIB
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