Toll-like Receptor 4, F4/80 And Pro-inflammatory Cytokines In Intestinal And Mesenteric Fat Tissue Of Crohn's Disease.

Crohn's disease (CD) is a chronic intestinal ailment with a multifactorial etiology, whose incidence has increased during the last three decades. Recently, a role for mesenteric fat has been proposed in CD pathophysiology, since fat hypertrophy is detected nearby the affected intestinal area; however, there are few studies on this aspect. To evaluate inflammatory activity in intestinal mucosa and mesenteric fat tissue of patients with CD and controls. Ten patients with ileocecal CD and 16 patients with non-inflammatory disease (control groups) were studied. The specimens were snap-frozen and the expression of TLR-4, F4/80, IL1-β and IL-6 were determined by immunoblot of protein extracts. TLR4 RNA level were measured using RT-PCR. The t Test was applied (p<0.05). The local ethical committee approved the study. The intestinal mucosa of CD group had significantly higher protein levels of TLR-4, F4/80, IL-1β and IL-6 than the controls. The gene expression of TLR4 was lower in the intestinal mucosa of CD compared to the control group. Regard the mesenteric fat tissue, there was no statistical difference related to TLR-4, F4/80, IL-1β and IL-6 proteins expression. These findings may result from an up-regulation of macrophage activation and intracellular pathways activated by bacterial antigens, which are more important in intestinal mucosa than fat tissue in CD patients. This may represent an anomalous regulation of innate immunity and could contribute to the production of proinflammatory mediators and disease development.698-10

LABORATÓRIO DE BIOMECÂNICA DO PORTO: ENSINO, INVESTIGAÇÃO E SERVIÇOS À COMUNIDADE

Porto Biomechanics Laboratory is a technological centre of the University of Porto dedicated to teaching, scientific and technological research, innovation, to provide specialized technical services and transference of knowledge and technology (product development) in Biomechanics (human and animal) of ergonomic, clinical, sports or biomimetic interest (e.g. design, computer animation and model development). LABIOMEP-UP mission is to catalyse the growth potential and competitive edge of University of Porto in all domains directly or indirectly related to Biomechanics, including the interaction with industry.El Laboratorio de Biomecánica de Porto es un centro tecnológico de la Universidad de Porto dedicado a la docencia, la investigación científica y tecnológica, la innovación, proporcionar servicios técnicos especializados y transferencia de conocimiento y tecnología (desarrollo de productos) en Biomecánica (humana y animal) de ergonomía, clínica, deportiva, o interés biomimético (por ejemplo, diseño, animación por computadora y desarrollo de modelos). La misión de LABIOMEP-UP es catalizar el potencial de crecimiento y la ventaja competitiva de la Universidad de Oporto en todos los dominios relacionados directa o indirectamente con la biomecánica, incluida la interacción con la industria.O Laboratório de Biomecânica do Porto (LABIOMEP-UP) é um centro de competências da Universidade do Porto dedicado ao ensino, investigação científica e tecnológica, inovação, prestação de serviços técnicos especializados e transferência de conhecimento e tecnologia em Biomecânica (humana e animal) de interesse ergonómico, clínico, desportivo ou biomimético (ex: design, animação computacional e desenvolvimento de modelos). A missão do LABIOMEP-UP é catalisar o potencial de crescimento e vantagem competitiva da Universidade do Porto em todos os domínios direta ou indiretamente relacionados com a Biomecânica, incluindo a interação com o tecido industrial e comercial

LABORATÓRIO DE BIOMECÂNICA DO PORTO: ENSINO, INVESTIGAÇÃO E SERVIÇOS À COMUNIDADE

Porto Biomechanics Laboratory is a technological centre of the University of Porto dedicated to teaching, scientific and technological research, innovation, to provide specialized technical services and transference of knowledge and technology (product development) in Biomechanics (human and animal) of ergonomic, clinical, sports or biomimetic interest (e.g. design, computer animation and model development). LABIOMEP-UP mission is to catalyse the growth potential and competitive edge of University of Porto in all domains directly or indirectly related to Biomechanics, including the interaction with industry.El Laboratorio de Biomecánica de Porto es un centro tecnológico de la Universidad de Porto dedicado a la docencia, la investigación científica y tecnológica, la innovación, proporcionar servicios técnicos especializados y transferencia de conocimiento y tecnología (desarrollo de productos) en Biomecánica (humana y animal) de ergonomía, clínica, deportiva, o interés biomimético (por ejemplo, diseño, animación por computadora y desarrollo de modelos). La misión de LABIOMEP-UP es catalizar el potencial de crecimiento y la ventaja competitiva de la Universidad de Oporto en todos los dominios relacionados directa o indirectamente con la biomecánica, incluida la interacción con la industria.O Laboratório de Biomecânica do Porto (LABIOMEP-UP) é um centro de competências da Universidade do Porto dedicado ao ensino, investigação científica e tecnológica, inovação, prestação de serviços técnicos especializados e transferência de conhecimento e tecnologia em Biomecânica (humana e animal) de interesse ergonómico, clínico, desportivo ou biomimético (ex: design, animação computacional e desenvolvimento de modelos). A missão do LABIOMEP-UP é catalisar o potencial de crescimento e vantagem competitiva da Universidade do Porto em todos os domínios direta ou indiretamente relacionados com a Biomecânica, incluindo a interação com o tecido industrial e comercial

Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis

<p>Abstract</p> <p>Background</p> <p>Ileal pouch-anal anastomosis (IPAA) is the surgical procedure of choice for patients with refractory ulcerative colitis (UC) and for familial adenomatous polyposis (FAP) with many rectal polyps. Pouchitis is one of the more frequent complications after IPAA in UC patients; however, it is rare in FAP.</p> <p>Objective</p> <p>Evaluate pro-apoptotic activity in endoscopically and histological normal mucosa of the ileal pouch in patients with UC and FAP.</p> <p>Methods</p> <p>Eighteen patients (nine with UC and nine with FAP) with J pouch after total rectocolectomy were studied. Biopsies were obtained from the mucosa of the pouch and from normal ileum. The specimens were snap-frozen and the expressions of Bax and Bcl-2 were determined by immunoblot of protein extracts and by immunohistochemistry analysis. FADD, Caspase-8, APAF-1 and Caspase-9 were evaluated by immunoprecipitation and immunoblot.</p> <p>Results</p> <p>Patients with UC had significantly higher protein levels of Bax and APAF-1, Caspase-9 than patients with FAP, but were similar to controls. The expressions of Bcl-2 and FADD, Caspase-8 were similar in the groups. Immunohistochemistry for Bax showed less intensity of immunoreactions in FAP than in UC and Controls. Bcl-2 immunostaining was similar among the groups.</p> <p>Conclusion</p> <p>Patients with FAP present lower levels of pro-apoptotic proteins in all methods applied, even in the absence of clinical and endoscopic pouchitis and dysplasia in the histological analysis. These findings may explain a tendency of up-regulation of apoptosis in UC patients, resulting in higher rates of progression to pouchitis in these patients, which could correlate with mucosal atrophy that occurs in inflamed tissue. However, FAP patients had low pro-apoptotic activity in the mucosa, and it could explain the tendency to low cell turn over and presence of adenomas in this syndrome.</p

Antinociceptive action and redox properties of citronellal, an essential oil present in lemongrass

This is a copy of an article published in the Journal of Medicinal Food© 2011 - copyright Mary Ann Liebert, Inc.; Journal of Medicinal Food is available online at: http://online.liebertpub.comCitronellal (CT) is a monoterpenoid and the major constituent of the mixture of terpenoids that give the citronella oil its lemon scent. Citronella oil is widely used around the world for various purposes and is mainly obtained from plants of the Cymbopogon genus, which are known as “lemongrass.” Considering these plants have been used worldwide for various medicinal purposes, the interest of researchers to understand the biological activities of monoterpenoids related to the Cymbopogon genus has been increasing. In the present work, we investigated the antinociceptive action and the redox properties of CT. Our results indicate that intraperitoneal injection of CT was effective in reducing nociceptive face-rubbing behavior in both phases of the formalin test, which was also naloxone-sensitive. CT also evoked antinociceptive response in the capsaicin and glutamate tests. The total radical-trapping antioxidant parameter and total antioxidant reactivity assays indicate that CT at doses of 0.1 and 1 mg/mL exerts a significant antioxidant activity, which is probably related to its ability to scavenge superoxide and nitric oxide, but not H2O2 or hydroxyl radicals, as evaluated separately by specific in vitro tests. These results show for the first time the antinociceptive potential of CT and indicate that the antioxidant properties of this compound may rely on its mechanism of biological actions because CT-containing natural products are used to treat various diseases related to oxidative stress and reactive species