The relationship between chronic sleep deficits and distractions in young adult drivers

This study reports the relationship between chronic sleep deficits and distractions on driving mistakes in university undergraduates (age 19-23 years). All participants were asked to complete a questionnaire about their sleeping habits and to drive a fixed-base driving simulator housed in a Fiat Cinquencento. Drivers were distracted either by being asked to read a map, operate a radio, take a drink, open a sweet wrapper or discuss with the experimenter (on a mobile phone during the drive).The results showed that drivers had more speed limit exceedances and more road edge excursions when distracted. There was also a significant difference in speed exceedances between participants who had sufficient sleep and those that had a chronic sleep debt. Significant positive correlations were found between speed exceedance and obtaining too little sleep, and between feeling uncomfortable during the day in the distracted drivers. There was also a significant negative correlation between speed exceedances and actual hours of sleep. Even when not distracted, a positive correlation existed between the number of collisions and difficulty in waking up. The results of this study indicate that young drivers with chronic sleep deficits are more likely to make driving errors when distracted

Comparative in-vitro studies on the efficacy of ivermectin against gastrointestinal sheep nematode

Purpose: This study was designed to evaluate the relative efficacy of various brands of ivermectin injection available for use in clinical veterinary practice in Nigeria. Method: Ivermectin injections were evaluated by a larval development assay (LDVA), using the larvae of Strongyles (predominantly Haemonchus contortus) of sheep. The effect of standard solutions of the drug from the various brands on the transformation of L1 to L3 and survival of L3 larvae was used to assess bioactivity. The 50% lethal concentration (LC50) was determined from regression line obtained by probit transformation of the biological data. The LC50 values for each of the brands were compared with that of the innovator brand (Ivomec Superâ) for any significant difference. Results: The LC50 values obtained for the five brands varied widely. It ranges from 1.1±0.17 ng/ml for the innovator brand to 2.3±0.3, 3.0±0.3, 8.0±0.2 and 17.0±0.3 ng/ml for the other four brands. The biological assays performed on each of the five brands were of comparable precision. LC50 for Ivomec superâ was significantly different from those of the other four brands (Student's t test, p < 0.01). Conclusion: The bioactivities of brands of ivermectin injections available in Nigeria are significantly different. This is a probable reason for the varied treatment response to various brands of ivermectin injection in veterinary practice in Nigeria. This justifies the need for drug regulatory bodies in Nigeria to ensure that ivermectin injections registered for use in Nigeria meets approved standards before the drugs are allowed to be imported into the country. Key words: Bioactivity, ivermectin, sheep nematode Trop J Pharm Res, December 2003; 2(2): 235-23

ATRIAL FIBRILLATION IN PATIENT WITH DIABETES MELLITUS 2 TYPE: CO-EXISTANCE AND THERAPEUTIC APROCHES

On the example of the clinical case of atrial fibrillation in patient with diabetes mellitus type 2, were discussed molecular mechanisms and therapeutic perspectives, anticoagulation treatment and anti-arrhythmic treatment strategies benefit. Recommendations on lifestyle modification and medicament treatment tactics are described

Dataset on noise level measurement in Ota metropolis, Nigeria

Datasets contained in this article are noise level measurementcarried out at 41 different locations in Ota metropolis, Nigeria. Thenoise readings were measured at a time interval of 30 min for eachsite considered using a precision grade sound level meter. Theanalysis was based on the noise descriptors LAeq,L10,L90,LD, TNIand NEI. Results from the study reflects that the highest and lowestequivalent noise levels (LAeq) were recorded at commercial areas(96 dB (A)) and residential areas (52 dB (A)), respectively, thebackground noise level (L90) has the highest and lowest values atcommercial areas (77 dB (A)) and residential areas (44 dB (A)),respectively and the peak value (L10) has the highest value andlowest value at the commercial areas (96 dB (A)) and residentialareas (56 dB (A)). Based on the WHO recommendations and stan-dards, only 2 out of the 41 locations considered are under normallyacceptable situation while the noise levels of other areas are notacceptable. Noise map developed in this study provides enoughinformation for technical controls and interim legislation againstenvironmental noise pollution in the metropolis. Moreover, con-sidering the noise emission standards, planning and promoting thecitizens awareness about the high noise risk could help to mitigatethe effect of noise in Ota, Metropolis. The noise data in this study are useful as reference and guideline for future regulations onnoise limit to be implemented for urban areas in Nigeria anddeveloping countries at large

Transactivation of EGFR by LPS induces COX-2 expression in enterocytes

Necrotizing enterocolitis (NEC) is the leading cause of gastrointestinal morbidity and mortality in preterm infants. NEC is characterized by an exaggerated inflammatory response to bacterial flora leading to bowel necrosis. Bacterial lipopolysaccharide (LPS) mediates inflammation through TLR4 activation and is a key molecule in the pathogenesis of NEC. However, LPS also induces cyclooxygenase-2 (COX-2), which promotes intestinal barrier restitution through stimulation of intestinal cell survival, proliferation, and migration. Epidermal growth factor receptor (EGFR) activation prevents experimental NEC and may play a critical role in LPS-stimulated COX-2 production. We hypothesized that EGFR is required for LPS induction of COX-2 expression. Our data show that inhibiting EGFR kinase activity blocks LPS-induced COX-2 expression in small intestinal epithelial cells. LPS induction of COX-2 requires Src-family kinase signaling while LPS transactivation of EGFR requires matrix metalloprotease (MMP) activity. EGFR tyrosine kinase inhibitors block LPS stimulation of mitogen-activated protein kinase ERK, suggesting an important role of the MAPK/ERK pathway in EGFR-mediated COX-2 expression. LPS stimulates proliferation of IEC-6 cells, but this stimulation is inhibited with either the EGFR kinase inhibitor AG1478, or the selective COX-2 inhibitor Celecoxib. Taken together, these data show that EGFR plays an important role in LPS-induction of COX-2 expression in enterocytes, which may be one mechanism for EGF in inhibition of NEC

HLA-DQA1*05 carriage associated with development of anti-drug antibodies to infliximab and adalimumab in patients with Crohn's Disease

Anti-tumor necrosis factor (anti-TNF) therapies are the most widely used biologic drugs for treating immune-mediated diseases, but repeated administration can induce the formation of anti-drug antibodies. The ability to identify patients at increased risk for development of anti-drug antibodies would facilitate selection of therapy and use of preventative strategies.This article is freely available via Open Access. Click on Publisher URL to access the full-text

Short communication - A PRELIMINARY INVESTIGATION OF RESISTANCE TO ANTHELMINTICS IN STRONGYLES OF CATTLE IN SHAKI, NIGERIA

A survey was conducted and the occurrence of anthelmintic resistance in strongyles of cattle was detected. Ten herds of cattle in Shaki, Oyo state of Nigeria were studied. Larval Development Assay (LDA) was used as the test method. Four drugs were tested. Resistance to the four drugs were detected. Resistance to albendazole were detected in two herds and in one herd for febantel with LD50 > 0.10 μgml -1 . Resistance to levalmisole and morantel was detected in three and in two herds respectively with LD50 >1.0μgml -

BIOSEPARATION AND ACTIVITY OF KHAYA SENEGALENSIS FRACTIONS AGAINST INFECTIVE LARVAE OF HAEMONCHUS CONTORTUS

The anthelmintic constituents of Khaya senegalensis (Deser.) A. Juss (Meliaceae) bark extract, previously demonstrated to show both in vitro and in vivo activity against gastrointestinal nematodes of sheep were investigated by chromatographic separation of the crude extract and bioassay of fractions. Bioseparation of the crude ethanol (95%) extract was carried out by gradient vacuum liquid chromatographic analysis and thin layer chromatography fingerprinting of eluates. The activities of the fractions were tested by a larval development assay in vitro. The survival rate of infective larvae L3 of sheep nematodes, predominantly Haemonchus contortus, was used to assess relative bioactivity. A secondary fraction obtained from further purification by preparative thin layer chromatography of a primary active fraction was also assessed for bioactivity. The compositions of the fractions were determined by qualitative chemical tests. The extracts killed infective larvae of H. contortus in a concentration-dependent manner. Best-fit LC50 values are 80.81, 63.73, 44.03 and 63.90 µg/ml for fractions A, B, C, and D, respectively (95% CI). The fractions are composed of saponins (A), saponins and alkaloids (B) saponins, terpenoids, flavonoids, condensed tannins (C), and saponins and tannins (D). Fraction C showed the highest activity of all the fractions (p < 0.0001, 1-way ANOVA, Bartlett’s test). The secondary fraction, C1A obtained from fraction C gave best-fit LC50 value of 5.09 µg/ml (95% CI) and was identified to be condensed tannin. The condensed tannins in the bark of K. senegalensis are the most potent anthelmintic principles of the extract

Anthelmintic Efficacy of Nauclea Latifolia Extract Against Gastrointestinal Nematodes of Sheep: In Vitro and In Vivo Studies

Direct effects of Nauclea latifolia extracts on different gastrointestinal nematodes of sheep is described. In vivo and in vitro studies were conducted to determine possible anthelmintic effect of leaf extracts of Nauclea latifolia toward different ovine gastro intestinal nematodes. A larval development assay was used to investigate in vitro, the effect of aqueous and ethanolic extracts of N. latifolia towards strongyles larvae. The development and survival of infective larvae (L3) was assessed and best-fit LC50 values were computed by global model of non-linear regression analysis curve-fitting (95% CI). Twenty sheep harbouring naturally acquired gastrointestinal nematodes were treated with oral administration of ethanolic extracts at a dose rate of 125 mg/kg, 250 mg/kg and 500mg/kg to evaluate therapeutic efficacy, in vivo