Functional diversity of 2-oxoglutarate/Fe(II)-dependent dioxygenases in plant metabolism

Oxidative enzymes catalyze many different reactions in plant metabolism. Among this suite of enzymes are the 2-oxoglutarate/Fe(II)-dependent dioxygenases (2-ODDs). Cytochromes P450 (CYPs) as often considered the most versatile oxidative enzymes in nature, but the diversity and complexity of reactions catalyzed by 2-ODDs is superior to the CYPs. The list of oxidative reactions catalyzed by 2-ODDs includes hydroxylations, demethylations, desaturations, ring closure, ring cleavage, epimerization, rearrangement, halogenation, and demethylenation. Furthermore, recent work, including the discovery of 2-ODDs involved in epigenetic regulation, and others catalyzing several characteristic steps in specialized metabolic pathways, support the argument that 2-ODDs are among the most versatile and important oxidizing biological catalysts. In this review, we survey and summarize the pertinent literature with a focus on several key reactions catalyzed by 2-ODDs, and discuss the significance and impact of these enzymes in plant metabolism

Nonresidential Fathers Parenting Their Children Residing in Shelters: A Phenomenological Study

This phenomenological qualitative study explored the parenting role of nonresidential fathers of children living in shelters. Special attention was paid to the perceived contributions of these fathers to the overall health and general well-being of their children residing in shelters. Often separations of nonresidential fathers from their children in shelters decreased their contributions to their children\u27s health and well-being. Increased knowledge of these parental roles and contributions can enhance programs and policies to support these fathers in improving the health and well-being of their children. In-depth semistructured interviews were conducted with 6 demographically diverse nonresidential fathers living in Philadelphia. The health-belief model, in conjunction with the revised health-belief model, was used as a theoretical framework for this study. The research questions were designed to explore nonresidential fathers\u27 parenting roles, perceptions of their contributions, and the facilitators of and barriers to their parenting while their children resided in shelters. An inductive approach to data analysis informed study findings of nonresidential fathers\u27 active participation and engagement in their children\u27s lives, including involvement in their healthcare and health promotion. Perceived facilitators to their parenting role included internal and external motivators, whereas perceived challenges and barriers to their parenting role were externally based. Finally, study findings showed these fathers to be present and making significant contributions to the improved health and overall well-being of their children while they resided in homeless shelters

A new clinical tool for assessing numerical abilities in neurological diseases: numerical activities of daily living

The aim of this study was to build an instrument, the numerical activities of daily living (NADL), designed to identify the specific impairments in numerical functions that may cause problems in everyday life. These impairments go beyond what can be inferred from the available scales evaluating activities of daily living in general, and are not adequately captured by measures of the general deterioration of cognitive functions as assessed by standard clinical instruments like the MMSE and MoCA. We assessed a control group (n = 148) and a patient group affected by a wide variety of neurological conditions (n = 175), with NADL along with IADL, MMSE, and MoCA. The NADL battery was found to have satisfactory construct validity and reliability, across a wide age range. This enabled us to calculate appropriate criteria for impairment that took into account age and education. It was found that neurological patients tended to overestimate their abilities as compared to the judgment made by their caregivers, assessed with objective tests of numerical abilities

Surface and subsurface rolling contact fatigue characteristic depths and proposal of stress indexes

The rolling contact fatigue is distinguished into subsurface initiated (spalling and case crushing) and surface initiated (pitting and micropitting). A characteristic depth was identified for each of these mechanism. The characteristic depth of the case crushing is the hardening depth, while for the spalling it is the maximum cyclic shear stress depth. The pitting depth is the size of the crack for which the mode I stress intensity factor range, due to the fluid pressurization, is higher than the threshold. This depth can be similar to the micropitting depth, in the order of 10 µm, for heavily loaded small radius contacts. Rolling contact fatigue cyclic shear stress indexes are then defined on the basis of the characteristic depths, and they identify the load intensity of each rolling contact fatigue mechanism. The characteristic depths and the stress index approach can be used to relate specific tests to component design, without any size effect misinterpretation

Glutathione-loaded solid lipid microparticles as innovative delivery system for oral antioxidant therapy

The present study aimed to develop a novel formulation containing glutathione (GSH) as an oral antioxidant therapy for the treatment of oxidative stress-related intestinal diseases. To this purpose, solid lipid microparticles (SLMs) with Dynasan 114 and a mixture of Dynasan 114 and Dynasan 118 were produced by spray congealing technology. The obtained SLMs had main particle sizes ranging from 250 to 355 µm, suitable for oral administration. GSH was efficiently loaded into the SLMs at 5% or 20% w/w and the encapsulation process did not modify its chemico-physical properties, as demonstrated by FT-IR, DSC and HSM analysis. Moreover, in vitro release studies using biorelevant media showed that Dynasan 114-based SLMs could efficiently release GSH in various intestinal fluids, while 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay demonstrated the good radical scavenging activity of this formulation. Dynasan 114-based SLMs exhibited an excellent biocompatibility on intestinal HT-29 cells at concentrations up to 2000 µg/mL. SLMs containing GSH alone or together with another antioxidant agent (catalase) were effective in reducing intracellular reactive oxygen species (ROS) levels. Overall, this study indicated that spray congealed SLMs are a promising oral drug delivery system for the encapsulation of one or more biological antioxidant agents for local intestinal treatment

Impact of wolf (Canis lupus) on animal husbandry in an Apennine province

Predation has always been an important problem in extensive sheep farms, causing serious economic losses to the farmers. In the Province of Lucca, the presence of reproductive wolf packs has already been confirmed in natural reserves, but occasional signs of presence of the predator have been reported also in neighbouring areas. The present research has been carried out in this Province (between the Orecchiella Natural Reserve and the medium Serchio Valley), in order to obtain more complete information on the location of the wolf (with transects, wolf- howling and snow-tracking), and to verify the real impact and risk factors of predation on live- stock (by means of on-farm surveys carried out in 42 semi-extensive farms) in this area. The presence of wolf was confirmed in the study area with a minimum of four adult individuals and at least one pup: this pack lives around the peaks of the Apennines in the municipalities covered by this investigation. A growing conflict between the wolf and the sheep and goat farms was observed: since 2007 there have been 25 attacks and three farms can be considered subject to chronic predation. The major risk factors are high altitude, large flock size and lack of fences and of guardian dogs. An accurate knowledge of wolf presence and the identification of the farms mostly at risk can be useful for future planning of interventions aimed at prevention and support of farmers, in order to mitigate the conflict caused by predation

Exploring the dimming event of RW Aur A through multi-epoch VLT/X-Shooter spectroscopy

RW Aur A is a CTTS that has suddenly undergone three major dimming events since 2010. We aim to understand the dimming properties, examine accretion variability, and derive the physical properties of the inner disc traced by the CO ro-vibrational emission at NIR wavelengths (2.3 mic). We compared two epochs of X-Shooter observations, during and after the dimming. We modelled the rarely detected CO bandhead emission in both epochs to examine whether the inner disc properties had changed. The SED was used to derive the extinction properties of the dimmed spectrum and compare the infrared excess between the two epochs. Lines tracing accretion were used to derive the mass accretion rate in both states. The CO originates from a region with physical properties of T=3000 K, N$_{CO}$=1x10$^{21}$ cm$^{-2}$ and vsini=113 km/s. The extinction properties of the dimming layer were derived with the effective optical depth ranging from teff 2.5-1.5 from the UV to the NIR. The inferred mass accretion rate Macc is $1.5x 10^{-8}$ Msun/yr and $\sim 2x 10^{-8}$ Msun/yr after and during the dimming respectively. By fitting the SED, additional emission is observed in the IR during the dimming event from dust grains with temperatures of 500-700K. The physical conditions traced by the CO are similar for both epochs, indicating that the inner gaseous disc properties do not change during the dimming events. The extinction curve is flatter than that of the ISM, and large grains of a few hundred microns are thus required. When we correct for the observed extinction, Macc is constant in the two epochs, suggesting that the accretion is stable and therefore does not cause the dimming. The additional hot emission in the NIR is located at about 0.5 au from the star. The dimming events could be due to a dust-laden wind, a severe puffing-up of the inner rim, or a perturbation caused by the recent star-disc encounter.Comment: Accepted by Astronomy & Astrophysic

Does exist a correlation between endometriosis and thrombophilic disorders? A pilot study

OBJECTIVE: At present, there is growing evidence of the existence of a genetic predisposition in both thrombophilic disorders and endometriosis. The aim of our study was to evaluate for the first time the prevalence of some thrombophilic disorders in patients with endometriosis. MATERIALS AND METHODS: We conducted a retrospective study on 138 patients with endometriosis and 278 healthy control women. All women were subjected to a blood examination testing for thrombophilic screening and the variables examinated were: hyperhomocysteinemia, factor V Leiden and factor II prothrombin G20210A mutations in heterozygosis and homozigosis. RESULTS: A significant reduced prevalence (p < 0.05) of factor V Leiden mutation in endometriosis patients was found, whereas no significant differences (p = NS) for factor II and hyperhomocysteinemia were observed. CONCLUSION: Our preliminary data do not show any association between thrombophilic condition and endometriosis. Before assuming hormonal therapies, a thrombophilic plasmatic screening seems to be unnecessary in patients affected by endometriosis