Ganciclovir penetrates into the cerebrospinal fluid of an infant with congenital cytomegalovirus infection

Currently, there is no evidence whether ganciclovir, or its oral prodrug valganciclovir, penetrates into the cerebrospinal fluid of human infants treated for congenital cytomegalovirus infection. Here we report a case study providing evidence that ganciclovir, administered as valganciclovir, reaches the infant's cerebrospinal fluid when used at the currently recommended dose for congenital cytomegalovirus infection

High‐Resolution Seismic Imaging of Fault‐Controlled Basins: A Case Study From the 2009 Mw 6.1 Central Italy Earthquake

We present the first seismic reflection images of the Paganica and Bazzano basins, two tectonic basins developed in the hanging wall of the Paganica-San Demetrio Fault System, the causative fault of the 2009 Mw 6.1 L'Aquila earthquake, Italy. Five high-resolution seismic profiles were acquired along a main, 7 km long transect cutting across the strands of an active fault system in urbanized areas with widespread sources of seismic noise. Three processing approaches were chosen to tackle a variable and site-dependent data quality . To aid interpretation of this complex setting, we complemented seismic amplitude images with energy and similarity attributes as well with post-stack acoustic impedance inversion. The final seismic sections expose, with unprecedented resolution, the basins' structure and the uppermost splays of the 2009 earthquake. The seismic data show fine details of the subsurface stratigraphic setting, revealing continental depocenters carved in the marine Meso-Cenozoic substratum and displaced by a series of conjugate normal faults, mostly unknown before this study. Several of the imaged fault strands connect to the 2009 coseismic surface ruptures. Matching the seismic interpretation with constraints from surface geology and shallow boreholes, published data from field surveys and scientific drilling, we present a structural map of the Bazzano and Paganica basins with an estimation of the depth of the Meso-Cenozoic substratum. This map highlights a different structure, evolution, and age of the two basins, with the older Bazzano basin that likely began to form in late Pliocene

Control of redundant robot arms with null-space compliance and singularity-free orientation representation

This paper tackles the problem of controlling the position and orientation, expressed in a singularity-free representation form, of the end-effector of a redundant robot, while addressing an active compliant behaviour within the null-space. The manuscript extends the work in [1] by explicitly addressing the orientation part. In order to successfully accomplish the task, a dynamic controller is designed without need of any exteroceptive sensors information. A rigorous stability analysis is provided to confirm the developed theory. Experiments are finally carried out to bolster the performance of the proposed approach

The "Smart Ring" Experience in l'Aquila (Italy): Integrating Smart Mobility Public Services with Air Quality Indexes

This work presents the "City Dynamics and Smart Environment" activities of the Smart Ring project, a model for the smart city, based on the integration of sustainable urban transport services and environmental monitoring over a 4–5-km circular path, the "Smart Ring", around the historical center of l'Aquila (Italy). We describe our pilot experience performed during an experimental on-demand public service electric bus, "SmartBus", which was equipped with a multi-parametric air quality low-cost gas electrochemical sensor platform, "NASUS IV". For five days (28–29 August 2014 and 1–3 September 2014), the sensor platform was installed inside the SmartBus and measured air quality gas compounds (nitrogen dioxide, carbon oxide, sulfur dioxide, hydrogen sulfide) during the service. Data were collected and analyzed on the bases of an air quality index, which provided qualitative insights on the air status potentially experienced by the users. The results obtained are in agreement with the synoptic meteorological conditions, the urban background air quality reference measurements and the potential traffic flow variations. Furthermore, they indicated that the air quality status was influenced by the gas component NO 2 , followed by H 2 S, SO 2 and CO. We discuss the features of our campaign, and we highlight the potential, limitations and key factors to consider for future project designs

Assessing Shallow Soft Deposits through Near-Surface Geophysics and UAV-SfM: Application in Pocket Beaches Environments

This study employs a multimethod approach to investigate the sediment distribution in two pocket beaches, Ramla Beach and Mellieha S Beach, in Malta. Both study sites were digitally reconstructed using unmanned aerial vehicle (UAV) photogrammetry. For each case, an ERT and a dense network of ambient seismic noise measurements processed through a horizontal-to-vertical spectral ratio (HVSR) technique were acquired. Electrical resistivity tomography (ERT) analysis enables the estimation of sediment thickness in each beach. HVSR analysis revealed peaks related to beach sediments overlying limestone rocks in both sites and also indicated a deeper stratigraphic contact in Mellieha S Beach. Based on ERT measurements, sediment thickness is calculated for each HVSR measurement. Interpolation of results allows for bedrock surface modelling in each case study, and when combined with digital terrain models (DTMs) derived from photogrammetric models, sediment volumes are estimated for each site. The geometry of this surface is analyzed from a geological perspective, showing structural control of sediment distribution due to a normal fault in Mellieha S Beach and stratigraphic control facilitated by a highly erodible surface in Ramla Beach. The results emphasize the importance of adopting a three-dimensional perspective in coastal studies for precise sediment volume characterization and a deeper understanding of pocket beach dynamics. This practical multimethod approach presented here offers valuable tools for future coastal research and effective coastal management, facilitating informed decision making amidst the growing vulnerability of coastal zones to climate change impacts

The effect of four mutations on the expression of iduronate-2-sulfatase in mucopolysaccharidosis type II

AbstractMucopolysaccharidosis type II (Hunter syndrome; OMIM 309900) is a rare X-linked recessive lysosomal storage disorder caused by the deficiency of the enzyme iduronate-2-sulfatase (IDS; EC 3.1.6.13). Different alterations at the IDS locus, mostly missense mutations, have been demonstrated, by expression study, as deleterious, causing significant consequences on the enzyme function or stability. In the present study we report on the results of the transient expression of the novel K347T, 533delTT, N265I and the already described 473delTCC (previously named ΔS117) mutations in the COS 7 cells proving their functional consequence on IDS activity. This type of information is potentially useful for genotype–phenotype correlation, prognosis and possible therapeutic intervention

Physical properties and antimicrobial activity of bioactive film based on whey protein and Lactobacillus curvatus 54M16 producer of bacteriocins

The objective of the work was to study the viability and antimicrobial activity of bacteriocin-producing lactic acid bacteria (LAB) incorporated into whey protein/inulin/gelatine (WP) edible films in presence or absence of nutrient (modified MRS broth). Moreover, the role of the cell on the film structure and properties has been investigated. The results of the work showed that WP-based films were able to ensure a high viability of the bacteriocin-producing strain L. curvatus 54M16 during 28 days of storage at 4 �C. The addition of nutrient in the film matrix slightly affected the viability of the cells, but it was critical for the antimicrobial activity of the films. Films in presence of nutrient showed a good antimicrobial activity against L. innocua C6 as in vitro system as on cooked ham. The presence of LAB has a significant effect on the structure of the film: it reduced the viscosity of the film forming solution and improved the elasticity and the percentage of elongation. Whereas, no effect was observed for water vapour transmission rate and solubility. Thus, WP-based films in presence of modified MRS broth can be used as effective delivery and carrier systems for lactic acid bacteria to develop bioactive edible film or coating with antimicrobial properties

Severe pertussis infection in infants less than 6 months of age: clinical manifestations and molecular characterization

We conducted a study to determine the main traits of pertussis among unimmunized infants less than 6 months of age. From August 2012 to March 2015, 141 nasopharyngeal aspirates (NPAs) were collected from infants with respiratory symptoms attending 2 major hospitals in Rome. Clinical data were recorded and analyzed. Lab-confirmation was performed by culture and realtime PCR. B. pertussis virulence-associated genes (ptxP, ptxA and prn), together with multilocus variable-number tandem repeat analysis (MLVA), were also investigated by the sequence-based analysis on the DNAs extracted from positive samples. Antibiotic susceptibility with Etest was defined on 18 viable B. pertussis isolates. Samples from 73 infants resulted positives for B. pertussis. The median age of the patients was 45 d (range 7–165); 21 infants were treated with macrolides before hospital admission. Cough was reported for a median of 10 d before admission and 18 d after hospital discharge among infected infants, 84% of whom showed paroxysmal cough. No resistance to macrolides was detected. Molecular analysis identified MT27 as the predominant MLVA profile, combined with ptxP3-ptxA1-prn2 associated virulence genes. Although our data may not be generalized to the whole country, they provide evidence of disease severity among infants not vaccinated against pertussis. Moreover, genetically related B. pertussis strains, comprising allelic variants of virulence associated genes, were identified

Modelling soil water conent in a tomato field: proximal gamma ray spectroscopy and soil-crop system models

Proximal soil sensors are taking hold in the understanding of soil hydrogeological processes involved in precision agriculture. In this context, permanently installed gamma ray spectroscopy stations represent one of the best space-time trade off methods at field scale. This study proved the feasibility and reliability of soil water content monitoring through a seven-month continuous acquisition of terrestrial gamma radiation in a tomato test field. By employing a 1 L sodium iodide detector placed at a height of 2.25 m, we investigated the gamma signal coming from an area having a ~25 m radius and from a depth of approximately 30 cm. Experimental values, inferred after a calibration measurement and corrected for the presence of biomass, were corroborated with gravimetric data acquired under different soil moisture conditions, giving an average absolute discrepancy of about 2%. A quantitative comparison was carried out with data simulated by AquaCrop, CRITeRIA, and IRRINET soil-crop system models. The different goodness of fit obtained in bare soil condition and during the vegetated period highlighted that CRITeRIA showed the best agreement with the experimental data over the entire data-taking period while, in presence of the tomato crop, IRRINET provided the best results.Comment: 18 pages, 9 Figures, 3 Table

Best practices, challenges and innovations in pediatrics in 2019

This paper runs through key progresses in epidemiology, pathomechanisms and therapy of various diseases in children that were issued in the Italian Journal of Pediatrics at the end of last year. Novel research and documents that explore areas such as allergy, critical care, endocrinology, gastroenterology, infectious diseases, neonatology, neurology, nutrition, and respiratory tract illnesses in children have been reported. These observations will help to control childhood illnesses