Driving Process Innovation: A Structured Method for Improving Efficiency in SMEs☆

Abstract This paper proposes a method to drive process innovation toward the increase in efficiency of a production plant. The work defines a structured method, supported by a classification tool, to correctly organize whole plant information with a mayor focus on energy consumptions. The method was tested in a medium enterprise with the target to increase the efficiency of the entire production plant. The method is the basis for a web application tool. A correct data management permits to plan the best practices to improve processes and systems involved in terms of environmental and economic impacts, meaning a process sustainable innovation

optimization of energy efficiency of a production site a method to support data acquisition for effective action plans

Abstract Nowadays the efficient use of energy has acquired a significant importance in the industrial sector. Moreover, stringent regulations on environmental impact lead companies to tread a path towards energy efficiency in short terms to avoid penalties. The goal of this work is to propose a structured method to perform fast and simplified energy assessments. The latter starts from a proper classification of process data, passing through an effective mapping in order to identify criticalities that have to be solved by innovative action plans. Method will be tested on a real case study

Digital Manufacturing Systems: A Framework to Improve Social Sustainability of a Production Site

Abstract The topic of digital manufacturing is increasingly emerging in industry. One of the main scope of data digitalization is achieving more efficient factories. Different techniques and tools under the Industry 4.0 paradigm were already discussed in literature. These are aimed mostly at boosting company efficiency in terms of costs and environmental footprint. However, from a sustainability point of view, the social theme must be equally considered. While energy flows or costs can be already monitored in a production plant, this is not valid for data related to human effort. Monitoring systems aimed at supervising factory social sustainability were not already discussed in literature. The aim of this paper is to propose a method to acquire social related data in a production plant. The method is supported by a smart architecture within the concept of IoT factory. Such architecture permits to monitor the parameters that could influence social sustainability in a production site. After a discussion on production plants facilities and features, the parameters that need to be considered to guarantee socially sustainable manufacturing processes are identified. A set of sensors controls these data taken from different sources, including operator vital signs. Operations as well as humans are monitored. Data acquired by sensors are collected by a central server. A decision maker can interpret the data and improve the production system from a social point of view, implementing corrective actions. Data can be exploited not only for social assessments but even for other analyses on the production system. Guaranteeing social sustainability could boost the factory productivity. A case study is included in the paper: smart sensors are implemented in a production line to understand the operations efficiency in terms of social sustainability

an approach to favor industrial symbiosis the case of waste electrical and electronic equipment

Abstract Waste management, in particular waste electrical and electronic equipment (WEEE), currently represents an important issue for the modern society. A transition toward circular economy and industrial symbiosis models is needed to mitigate the environmental problem and recover value from end of life (EoL) materials/products. This study aims to define an approach and a platform, dedicated to the WEEE sector, to favor the creation of industrial symbiosis opportunities. Through this structured approach, demand of virgin materials and components, and supply of EoL products are linked to find potential collaborations. In this way, EoL components/materials could be reused in different applications, thus closed-loop lifecycles can be created through industrial symbiosis. A case study focused on the reuse of plastics from electrical cables is shown to demonstrate that in the WEEE sector the implementation of industrial symbiosis models can lead to win-win scenarios for all the involved stakeholders

improving a production site from a social point of view an iot infrastructure to monitor workers condition

Abstract In the context of Industry 4.0, this paper focuses on integration of workers in the digitalized factory. It proposes a method to design an IoT infrastructure and acquire human-related data from a production site in order to improve workers wellbeing and overall productivity. The method permits to identify bottlenecks and criticalities from a social point of view, focusing on the human performance, and define corrective actions at different levels (operations, plant layout or shift management). A case study was developed in collaboration with an Italian sole producer to validate the method and the related data acquisition system

637. Targeting of Myeloid Leukemia by IL-10-Engineered Human CD4+ Tr1 Cells

T regulatory type 1 (Tr1) cells, characterized by the co-expression of CD49b and LAG-3 and the ability to secrete high amounts of IL-10, control immune responses by IL-10 and TGF-beta production and by killing of myeloid cells via a Granzyme B-dependent mechanism. Tr1 cells are induced in vitro in the presence of recombinant human IL-10 or tolerogenic dendritic cells secreting high amounts of IL-10 (DC-10). Proof-of-principle clinical trials suggest that Tr1 cells can modulate Graft-versus Host Disease (GvHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, their ability to mediate anti-leukemic activity or their effects of Graft versus Leukemia is largely unknown. We previously showed that enforced IL-10 expression converts human CD4+ T cells into Tr1-like (CD4IL-10) cells that suppress effector T cells in vitro and prevent xenogeneic-GvHD in humanized models. We now demonstrate that these CD4IL-10 cells selectively kill myeloid cell lines and myeloid blasts in vitro in HLA-class I-dependent but antigen-independent manner. Moreover, cytotoxic activity of CD4IL-10 cells is Granzyme B-dependent, is specific for CD13+ cells, and requires CD54 and CD112 expression on target cell lines or primary leukemic blast. Adoptive transfer of CD4IL-10 cells in humanized models mediates direct anti-leukemic activity, and does not compromise the anti-leukemic effect of allogeneic T cells while inhibits xeno-GvHD. These findings provide a strong rationale for designing personalized immunotherapy approaches using CD4IL-10 cells after allo-HSCT to cure myeloid malignancies

Experimental and numerical investigations on historical masonry wall specimens tested in shear-compression configuration

The paper presents the experimental investigation carried out on wall specimens reproducing the ancient masonry of several monumental building located in the old city centre of L'Aquila (Italy) and damaged by the April 2009 earthquake. The wall specimens were prepared in accordance with the traditional technique, using original stone elements and typical poor mortar. Subsequently, the specimens were consolidated with mortar injections. Other specimens were also reinforced with Ultra High Tensile Strength Steel wires applied as coating technique (not wrapped). Shear-compression tests were carried out on the wall specimens to evaluate the effects of the reinforcements both in terms of final stiffness and strength of the specimens. A non-linear Finite Element Model (FEM) was developed to reproduce the experimental tests and to better understand the damage phenomena. The load-displacement curves predicted by the FEM compared quite well with the experimental ones. The failure mode of the specimens was properly captured by the FEM. The effectiveness of the external reinforcement was proved to strictly depend on the coating adhesiveness to the walls surface. The premature debonding of the external reinforcement was demonstrated to cause the fragile post-peak behaviour during both the actual experimental test and the numerical simulations

Ocorrência de coronavírus entérico de ferrets no Brasil: nota prévia

Ferret enteric coronavirus (FECV) is associated to the epizootic catarrhal enteritis (ECE) in ferrets (Mustela putorius furo). In this study, we report the occurrence of this agent in four diarrheic stool samples of domestic ferrets, analyzed by negative staining transmission electron microscopy and a specific RT-PCR assay targeting the nucleocapsid (N) gene. These findings are the first report of FECV in Brazil and address the importance of this virus on the etiology of enteric disorders in ferrets.Coronavírus entérico de furões (FECV) é associado à enterite catarral epizoótica (ECE) em furões (Mustela putorius furo). Neste estudo, relatamos a ocorrência deste agente em quatro amostras fecais diarreicas de furões domésticos, analisadas por microscopia eletrônica de transmissão (contrastação negativa) e RT-PCR específica e direcionada ao gene de nucleocapsídeo (N). Estes achados constituem o primeiro relato de FECV no Brasil e remetem para a importância deste vírus na etiologia de quadros entéricos nestes animais

Primer design for complete sequencing of the Avastrovirus ORF2 gene

Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention

Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil

The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus – LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT‑PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV‑infected lilies and nine fragments of 600 nt from LSV‑infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).O objetivo deste trabalho foi descrever a ocorrência do Tulip breaking virus (TBV, sinonímia Lily mottle virus – LMoV) quarentenário e do Lily symptomless virus (LSV) e suas análises moleculares, para fornecer dados para a remoção do TBV da lista brasileira de pragas quarentenárias A1, pois este vírus está disseminado pelos principais cultivos comerciais de lírio no Brasil. A ocorrência desses vírus foi detectada em 12 regiões produtoras, por meio da reação de transcrição reversa multiplex (RT‑PCR), com uso de iniciadores específicos para os genes das respectivas capas proteicas (CP). Foram sequenciados oito fragmentos de 800 nucleotídeos (nt), obtidos de lírios infectados com LMoV, e nove fragmentos de 600 nt, obtidos de lírios infectados com LSV. A reconstrução da árvore filogenética mostrou um ramo robusto que continha as sequências brasileiras de LMoV, outros isolados de LMoV, TBV e Tulip band breaking virus, o que indica que todos são isolados de LMoV, embora agrupados em dois subgrupos. A análise filogenética também mostrou um ramo robusto que suporta todas as sequências brasileiras e outras de LSV, com exceção de um isolado japonês de LSV. Análises de recombinação também mostraram um isolado recombinante de LMoV, enquanto nenhum evento de recombinação foi detectado em LSV. Lily mottle virus é prevalente em cultivos de lírios no Brasil, em infecções simples e mistas com LSV ou Cucumber mosaic virus (CMV)