13 research outputs found
ChIA-PET tool for comprehensive chromatin interaction analysis with paired-end tag sequencing
ChIA-PET Tool can be used to process long-range chromatin interaction data. Results are visualized on a user-friendly genome browser
Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces Loss of Epithelial Integrity.
Genome rearrangements, a hallmark of cancer, can result in gene fusions with oncogenic properties. Using DNA paired-end-tag (DNA-PET) whole-genome sequencing, we analyzed 15 gastric cancers (GCs) from Southeast Asians. Rearrangements were enriched in open chromatin and shaped by chromatin structure. We identified seven rearrangement hot spots and 136 gene fusions. In three out of 100 GC cases, we found recurrent fusions between CLDN18, a tight junction gene, and ARHGAP26, a gene encoding a RHOA inhibitor. Epithelial cell lines expressing CLDN18-ARHGAP26 displayed a dramatic loss of epithelial phenotype and long protrusions indicative of epithelial-mesenchymal transition (EMT). Fusion-positive cell lines showed impaired barrier properties, reduced cell-cell and cell-extracellular matrix adhesion, retarded wound healing, and inhibition of RHOA. Gain of invasion was seen in cancer cell lines expressing the fusion. Thus, CLDN18-ARHGAP26 mediates epithelial disintegration, possibly leading to stomach H(+) leakage, and the fusion might contribute to invasiveness once a cell is transformed. Cell Rep 2015 Jul 14; 12(2):272-285
Extensive Promoter-Centered Chromatin Interactions Provide a Topological Basis for Transcription Regulation
Higher-order chromosomal organization for transcription
regulation is poorly understood in eukaryotes.
Using genome-wide Chromatin Interaction
Analysis with Paired-End-Tag sequencing (ChIAPET),
we mapped long-range chromatin interactions
associated with RNA polymerase II in human cells
and uncovered widespread promoter-centered intragenic,
extragenic, and intergenic interactions. These
interactions further aggregated into higher-order
clusters, wherein proximal and distal genes were
engaged through promoter-promoter interactions.
Most genes with promoter-promoter interactions
were active and transcribed cooperatively, and
some interacting promoters could influence each
other implying combinatorial complexity of transcriptional
controls. Comparative analyses of
different cell lines showed that cell-specific chromatin
interactions could provide structural frameworks
for cell-specific transcription, and suggested
significant enrichment of enhancer-promoter interactions
for cell-specific functions. Furthermore,
genetically-identified disease-associated noncoding
elements were found to be spatially engaged with
corresponding genes through long-range interactions.
Overall, our study provides insights into transcription
regulation by three-dimensional chromatin
interactions for both housekeeping and cell-specific
genes in human cells
Chromatin connectivity maps reveal dynamic promoter-enhancer long-range associations.
In multicellular organisms, transcription regulation is one of the central mechanisms modelling lineage differentiation and cell-fate determination. Transcription requires dynamic chromatin configurations between promoters and their corresponding distal regulatory elements. It is believed that their communication occurs within large discrete foci of aggregated RNA polymerases termed transcription factories in three-dimensional nuclear space. However, the dynamic nature of chromatin connectivity has not been characterized at the genome-wide level. Here, through a chromatin interaction analysis with paired-end tagging approach using an antibody that primarily recognizes the pre-initiation complexes of RNA polymerase II, we explore the transcriptional interactomes of three mouse cells of progressive lineage commitment, including pluripotent embryonic stem cells, neural stem cells and neurosphere stem/progenitor cells. Our global chromatin connectivity maps reveal approximately 40,000 long-range interactions, suggest precise enhancer-promoter associations and delineate cell-type-specific chromatin structures. Analysis of the complex regulatory repertoire shows that there are extensive colocalizations among promoters and distal-acting enhancers. Most of the enhancers associate with promoters located beyond their nearest active genes, indicating that the linear juxtaposition is not the only guiding principle driving enhancer target selection. Although promoter-enhancer interactions exhibit high cell-type specificity, promoters involved in interactions are found to be generally common and mostly active among different cells. Chromatin connectivity networks reveal that the pivotal genes of reprogramming functions are transcribed within physical proximity to each other in embryonic stem cells, linking chromatin architecture to coordinated gene expression. Our study sets the stage for the full-scale dissection of spatial and temporal genome structures and their roles in orchestrating development. Nature 2013 Dec 12; 504(7479):306-10
<i>Xenopus tropicalis</i> Genome Re-Scaffolding and Re-Annotation Reach the Resolution Required for <i>In Vivo</i> ChIA-PET Analysis
<div><p>Genome-wide functional analyses require high-resolution genome assembly and annotation. We applied ChIA-PET to analyze gene regulatory networks, including 3D chromosome interactions, underlying thyroid hormone (TH) signaling in the frog <i>Xenopus tropicalis</i>. As the available versions of <i>Xenopus tropicalis</i> assembly and annotation lacked the resolution required for ChIA-PET we improve the genome assembly version 4.1 and annotations using data derived from the paired end tag (PET) sequencing technologies and approaches (e.g., DNA-PET [gPET], RNA-PET etc.). The large insert (~10Kb, ~17Kb) paired end DNA-PET with high throughput NGS sequencing not only significantly improved genome assembly quality, but also strongly reduced genome “fragmentation”, reducing total scaffold numbers by ~60%. Next, RNA-PET technology, designed and developed for the detection of full-length transcripts and fusion mRNA in whole transcriptome studies (ENCODE consortia), was applied to capture the 5' and 3' ends of transcripts. These amendments in assembly and annotation were essential prerequisites for the ChIA-PET analysis of TH transcription regulation. Their application revealed complex regulatory configurations of target genes and the structures of the regulatory networks underlying physiological responses. Our work allowed us to improve the quality of <i>Xenopus tropicalis</i> genomic resources, reaching the standard required for ChIA-PET analysis of transcriptional networks. We consider that the workflow proposed offers useful conceptual and methodological guidance and can readily be applied to other non-conventional models that have low-resolution genome data.</p></div
RNA-PET efficiently captures transcripts ends.
<p>A. Overlap between RNA-Seq reads and Ensembl and RNA-PET-based models. B. Demarcation of gene model boundaries by RNA-PET. The histogram shows the relative size of Ensembl gene models in bins of various sizes. C. Enrichment of RNA-Pol II around Ensembl gene models and RNA-PET-based models. This shows that RNA-Pol II density fits well with RNA-PET based models, but not Ensembl models.</p
Examples of genome annotation improvements.
<p>Track order: Ensembl models, RNA-PET based models, RNA-PET ditags and RNA-Seq reads density. A, B, C: <i>sumo1</i>, <i>cadm2</i> and <i>kiaa1958</i> loci. D: Un-annotated gene split over scaffold_1031 and scaffold_1460.</p
Benefit of genome re-annotation with RNA-PET for ChIA-PET analysis.
<p>A. Genomic view of an un-annotated gene. Track order: Ensembl genes, RNA-PET-based models, ChIA-PET TR binding density, RNA Pol-II binding density, RNA-Seq reads density with (+T<sub>3</sub>) and without (-T<sub>3</sub>) THs treatment. B. Close up of TR binding sites. Track order: Ensembl genes, RNA-PET-based genes, location of ChIP-qPCR probes, RNA-PET PETs, TR binding density and RNA Pol-II binding density. C: ChIP-qPCR validation of TR binding at locations shown in B. Ab: antibody, T<sub>3</sub>: 3’,5,3’ triiodothyronine treatment. D: Transcriptional induction assayed by RT-qPCR.</p
Benefit of genome re-annotation with RNA-PET for ChIA-PET analysis.
<p>A. Large genomic view of the <i>bcl6</i> locus. Track order: Ensembl genes, RNA-PET-based models, ChIA-PET TR binding density, interaction PETs, RNA Pol-II binding density, RNA-Seq reads density with (+T<sub>3</sub>) and without (-T<sub>3</sub>) treatment with thyroid hormones. B. Close up on TR binding sites. Track order: Ensembl genes, RNA-PET-based genes, location of ChIP-qPCR probes, RNA-PET ditags, TR binding density and RNA Pol-II binding density. C: ChIP-qPCR validation of TR binding at locations shown in B. Ab: Antibody, T<sub>3</sub>: 3’,5,3’ triiodothyronine treatment. D: Induction of <i>trpg1</i>, <i>lpp</i> and <i>bcl6</i> genes transcription assayed by RT-qPCR. E: Three-dimensional model of the locus topology.</p