Elaboração de um planejamento estratégico de uma empresa localizada na região norte do Paraná

Orientadores : Vanderlei Correia Gomes da Silva e Robson Leandro MafiolettiMonografia (especialização) - Universidade Federal do Paraná, Setor de ..., Curso de Especialização em ...Inclui referência

Resistência de união de pinos de fibra cimentados com diferentes materiais e técnicas de ativação à dentina radicular

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Odontologia, Florianópolis, 2011O sucesso clínico de um tratamento restaurador indireto depende de alguns fatores, dentre eles o tipo de cimento e a técnica de cimentação utilizada, que são responsáveis pela interação entre a restauração e o dente. Objetivou-se com este estudo avaliar, in vitro, a influência dos métodos de ativação (química, fotoativação convencional e pulso tardio) de um cimento resinoso auto adesivo e com condicionamento ácido total, sobre a resistência de união, sob push- out, de pinos de fibra fixados na dentina radicular. As coroas de sessenta pré-molares humanos hígidos foram cortados 1mm acima da junção cemento-esmalte, sendo o remanescente radicular padronizado em 16mm ± 1mm. Os dentes foram tratados endodonticamente e após uma semana os condutos preparados com brocas específicas para cimentação dos pinos. Os espécimes foram distribuídos aleatoriamente em 6 grupos (n=10), de acordo com o cimento utilizado e o método de ativação: G1 (RelyX ARC - fotoativação convencional), G2 (RelyX ARC - Ativação química), G3 ( RelyX ARC - fotoativação por pulso tardio), G4 (RelyX U 100 - fotoativação convencional), G5 (RelyX U100 - Ativação química) e G6 (RelyX U 100 - fotoativação por pulso tardio). Após uma semana de cimentação dos pinos, os espécimes foram seccionados em 6 fatias de 1 mm, sendo 2 fatias referentes a cada terço (cervical, médio e apical). Posteriormente, realizou-se o teste de cisalhamento por extrusão (push-out) em uma velocidade de 0,5mm/min. Os dados foram submetidos à análise de variância (ANOVA 2) e teste de Tukey. O cimento RelyX U 100 (Grupos 4, 5 e 6) apresentou, numericamente, maior resistência de união que o cimento RelyX ARC, em todas as técnicas de ativação, embora não tenha sido observada significância estatística na ativação química e na fotoativação por pulso tardio (p>0,05). No cimento RelyX ARC a ativação química apresentou menor valor de resistência de união que a ativação por pulso tardio. No cimento RelyX U 100 a ativação química apresentou menor valor de resistência de união que a fotoativação convencional. A técnica de fotoativação por pulso tardio não promoveu maiores valores de resistência de união em comparação a fotoativação convencional, dentro do mesmo grupo de cimento. Não houve diferenças estatísticas nos valores de resistência de união entre os terços radiculares, dentro de cada grupo.The clinical success of an indirect restorative treatment depends of some factors as the kind of material used as cement and the technique of cementation used. These factors are responsible by the interaction restoration and teeth. The aim of this study was to evaluate, in vitro, the influence of activation methods (chemical, conventional photoactivation and pulse delay) of a self adhesive and a total etch resin cement over the bond strength of fiber posts to root dentin, with push-out test. The clinical crowns of sixty higid human pre-molars were removed up to approximately 1.0 mm above the cement enamel junction, where the root length was standardized in 16mm ± 1mm. The teeth were endodontically treated and after one week the post spaces were prepared with a tapered drill specific for the post system. The specimens were divided randomly in 6 groups (n=10), according to the resin cement and activation methods used: G1 (RelyX ARC # Conventional photoactivation), G2 (RelyX ARC # Chemical), G3 (RelyX ARC # Pulse-delay), G4 (RelyX U 100 - Conventional photoactivation), G5 (RelyX U100 - Chemical) e G 6 (RelyX U 100 # Pulse-delay). One week after the posts cementation, the specimens were sectioned in 6 slices of 1 mm, where 2 slices are referring to each third (cervical, medium and apical). After that, it was done the extrusion shear a test (push-out) with speed of 0,5mm/min. The data were analyzed statistically using ANOVA 2 and Tukey test. The RelyX U 100 cement showed, numerically, higher bond strength than RelyX ARC cement in all activation methods, but statistical significance was observed only with the conventional photoactivation (p<0,05). With RelyX ARC cement, chemical activation showed lesser bond strength value than pulse delay photoactivation. With RelyX U 100 cement, chemical activation showed lesser bond strength value than conventional photoactivation. The pulse-delay activation didn't promote greater bond strength values when compared to conventional photoactivation to the same cement group. There were no statistical differences on bond strength values between the root thirds inside each group

Divergent Effect of Cobalt and Beryllium Salts on the Fate of Peripheral Blood Monocytes and T Lymphocytes

Occupational exposure to metals such as cobalt and beryllium represents a risk factor for respiratory health and can cause immune-mediated diseases. However, the way they act may be different. We show here that the two metals have a divergent effect on peripheral T lymphocytes and monocytes: BeSO4 induces cell death in monocytes but not in T lymphocytes, which instead respond by producing Interferon gamma (IFN-γ); conversely, CoCl2 induces apoptosis in T lymphocytes but not in monocytes. Interestingly, both metals induce p53 overexpression but with a dramatic different outcome. This is because the effect of p53 in CoCl2-treated monocytes is counteracted by the antiapoptotic activity of cytoplasmic p21Cip1/WAF1, the activation of nuclear factor κB, and the inflammasome danger signaling pathway leading to the production of proinflammatory cytokines. However, CoCl2-treated monocytes do not fully differentiate into macrophage or dendritic cells, as inferred by the lack of expression of CD16 and CD83, respectively. Furthermore, the expression of HLA-class II molecules, as well as the capability of capturing and presenting the antigens, decreased with time. In conclusion, cobalt keeps monocytes in a partially activated, proinflammatory state that can contribute to some of the pathologies associated with the exposure to this meta

The impact of the 'Mis-Peptidome' on HLA Class I-Mediated Diseases: contribution of ERAP1 and ERAP2 and effects on the immune response

The strong association with the Major Histocompatibility Complex (MHC) class I genes represents a shared trait for a group of autoimmune/autoinflammatory disorders having in common immunopathogenetic basis as well as clinical features. Accordingly, the main risk factors for Ankylosing Spondylitis (AS), prototype of the Spondyloarthropathies (SpA), the Behçet's disease (BD), the Psoriasis (Ps) and the Birdshot Chorioretinopathy (BSCR) are HLA-B*27, HLA-B*51, HLA-C*06:02 and HLA-A*29:02, respectively. Despite the strength of the association, the HLA pathogenetic role in these diseases is far from being thoroughly understood. Furthermore, Genome-Wide Association Studies (GWAS) have highlighted other important susceptibility factors such as Endoplasmic Reticulum Aminopeptidase (ERAP) 1 and, less frequently, ERAP2 that refine the peptidome presented by HLA class I molecules to CD8+ T cells. Mass spectrometry analysis provided considerable knowledge of HLA-B*27, HLA-B*51, HLA-C*06:02 and HLA-A*29:02 immunopeptidome. However, the combined effect of several ERAP1 and ERAP2 allelic variants could generate an altered pool of peptides accounting for the "mis-immunopeptidome" that ranges from suboptimal to pathogenetic/harmful peptides able to induce non-canonical or autoreactive CD8+ T responses, activation of NK cells and/or garbling the classical functions of the HLA class I molecules. This review will focus on this class of epitopes as possible elicitors of atypical/harmful immune responses which can contribute to the pathogenesis of chronic inflammatory diseases

HLA-E gene polymorphism associates with ankylosing spondylitis in Sardinia

Abstract INTRODUCTION: Ankylosing spondylitis (AS) is a severe, chronic inflammatory disease strongly associated with HLA-B27. The presence of additional HLA risk factors has been suggested by several studies. The aim of the current study is to assess the occurrence of an additional HLA susceptibility locus in the region between HLA-E and HLA-C in the Sardinian population. METHODS: 200 random controls, 120 patients with AS and 175 HLA-B27 positive controls were genotyped for six single nucleotide polymorphisms (SNPs) spanning the HLA region between HLA-E and HLA-C loci previously shown to harbour an additional susceptibility locus for AS. Allele, genotype and haplotype frequencies were compared. RESULTS: The data confirm our previous finding of a significant increase in patients with AS of allele A at SNP rs1264457 encoding for an Arg at the functional HLA-E polymorphism (Arg128/Gly128). This was due to a remarkable increase in the frequency of genotype A/A in patients vs HLA-B27-matched controls (51% vs 29%; P for trend: 5 x 10-5). Genotype distribution of three other SNPs mapping in genes (GNL1, PRR3 and ABCF-1) close to HLA-E and showing high LD with it, was also significantly skewed. Accordingly, haplotype distribution was also remarkably different. The frequency of the haplotype AAGA, is 42% in random controls, increases to 53% in the HLA-B27-positive controls, and reaches 68% in patients with AS (P values: 2 x 10-11 vs random and 3 x 10-4 vs HLA-B27 controls). CONCLUSIONS: There is a strong association between the presence of a haplotype in genes mapping between HLA-E and HLA-C and AS due to an increase of homozygous markers in patients. The strongest association however, is with the HLA-E functional polymorphism rs1264457. Since HLA-E is the ligand for the NKG2A receptor, these data point to the natural killer (NK) activity as possible player in the pathogenesis of AS

The Expression of Vasoactive Intestinal Peptide Receptor 1 Is Negatively Modulated by MicroRNA 525-5p

Background: The human Vasoactive Intestinal Peptide (VIP) is a neurokine with effects on the immune system where it is involved in promoting tolerance. In this context, one of its receptors, VPAC1, has been found to be down-modulated in cells of the immune network in response to activating stimuli. In particular, the bacterial liposaccaride (LPS), a strong activator of the innate immune system, induces a rapid decrease of VPAC1 expression in monocytes and this event correlates with polymorphisms in the 3'-UTR of the gene. Methodology/Principal Findings: MicroRNA 525-5p, having as putative target the 3'-UTR region of VPAC1, has been analysed for its expression in monocytes and for its role in down-modulating VPAC1 expression. We report here that miR-525-5p is promptly up-regulated in LPS-treated monocytes. This microRNA, when co-transfected in 293T cells together with a construct containing the 3'-UTR of the VPAC1 gene, significantly reduced the luciferase activity in a standard expression assay. The U937 cell line as well as primary monocytes enforced to express miR-525-5p, both down-modulate VPAC1 expression at similar extent. Conclusions/Significance: Our results show that the response to an inflammatory stimulus elicits in monocytes a rapid increase of miR-525-5p that targets a signaling pathway involved in the control of the immune homeostasis

VizieR Online Data Catalog: Hi-GAL. inner Milky Way: +68>=l>=70 (Molinari+, 2016)

This is the first public data release of high-quality products from the Herschel Hi-GAL survey. The release comes two years after the end of the Herschel observing campaign and is the result of extensive testing of the data reduction and extraction procedures created by members of the Hi-GAL consortium. The complexity and the large variation of the background conditions in all Herschel wavelength bands makes source extraction on the Galactic plane a challenging task. With Hi-GAL DR1, we provide access (http://vialactea.iaps.inaf.it) through a cutout service to high-quality images and compact source catalogues for the Galactic plane at 70, 160, 250, 350, and 500um in the region 68°>=l>=-70° and |b|<= 1°

Elaboração de um planejamento estratégico de uma empresa localizada na região norte do Paraná

Orientadores : Vanderlei Correia Gomes da Silva e Robson Leandro MafiolettiMonografia (especialização) - Universidade Federal do Paraná, Setor de ..., Curso de Especialização em ...Inclui referência