6,536 research outputs found
Diminishing Return for Increased Mappability with Longer Sequencing Reads: Implications of the k-mer Distributions in the Human Genome
The amount of non-unique sequence (non-singletons) in a genome directly
affects the difficulty of read alignment to a reference assembly for high
throughput-sequencing data. Although a greater length increases the chance for
reads being uniquely mapped to the reference genome, a quantitative analysis of
the influence of read lengths on mappability has been lacking. To address this
question, we evaluate the k-mer distribution of the human reference genome. The
k-mer frequency is determined for k ranging from 20 to 1000 basepairs. We use
the proportion of non-singleton k-mers to evaluate the mappability of reads for
a corresponding read length. We observe that the proportion of non-singletons
decreases slowly with increasing k, and can be fitted by piecewise power-law
functions with different exponents at different k ranges. A faster decay at
smaller values for k indicates more limited gains for read lengths > 200
basepairs. The frequency distributions of k-mers exhibit long tails in a
power-law-like trend, and rank frequency plots exhibit a concave Zipf's curve.
The location of the most frequent 1000-mers comprises 172 kilobase-ranged
regions, including four large stretches on chromosomes 1 and X, containing
genes with biomedical implications. Even the read length 1000 would be
insufficient to reliably sequence these specific regions.Comment: 5 figure
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Cities and population health.
A majority of the world's population will live in urban areas by 2007 and cities are exerting growing influence on the health of both urban and non-urban residents. Although there long has been substantial interest in the associations between city living and health, relatively little work has tried to understand how and why cities affect population health. This reflects both the number and complexity of determinants and of the absence of a unified framework that integrates the multiple factors that influence the health of urban populations. This paper presents a conceptual framework for studying how urban living affects population health. The framework rests on the assumption that urban populations are defined by size, density, diversity, and complexity, and that health in urban populations is a function of living conditions that are in turn shaped by municipal determinants and global and national trends. The framework builds on previous urban health research and incorporates multiple determinants at different levels. It is intended to serve as a model to guide public health research and intervention
The mystery of the writing that isn’t on the wall: differences in public representations in traditional and agile software development
This paper considers the use of public displays, such as whiteboards and papers pinned to walls, by different software development teams, based on evidence from a number of empirical studies. This paper outlines differences in use observed between traditional and agile teams and begins
to identify the implications that they may have for software
development
Choice, responsibility, and health: What role for the food movement?
https://scholarworks.uvm.edu/fss2014/1002/thumbnail.jp
From Lifestyle to Social Determinants: New Directions for Community Health Promotion Research and Practice
"... und Zwingli vor mir wie eine überhängende Wand": Karl Barths Wahrnehmung der Theologie Huldrych Zwinglis in seiner Göttinger Vorlesung von 1922/23
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