Translocating the blood-brain barrier using electrostatics

Copyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.Mammalian cell membranes regulate homeostasis, protein activity, and cell signaling. The charge at the membrane surface has been correlated with these key events. Although mammalian cells are known to be slightly anionic, quantitative information on the membrane charge and the importance of electrostatic interactions in pharmacokinetics and pharmacodynamics remain elusive. Recently, we reported for the first time that brain endothelial cells (EC) are more negatively charged than human umbilical cord cells, using zeta-potential measurements by dynamic light scattering. Here, we hypothesize that anionicity is a key feature of the blood-brain barrier (BBB) and contributes to select which compounds cross into the brain. For the sake of comparison, we also studied the membrane surface charge of blood components—red blood cells (RBC), platelets, and peripheral blood mononuclear cells (PBMC).To further quantitatively correlate the negative zeta-potential values with membrane charge density, model membranes with different percentages of anionic lipids were also evaluated. From all the cells tested, brain cell membranes are the most anionic and those having their lipids mostly exposed, which explains why lipophilic cationic compounds are more prone to cross the blood-brain barrier.Fundação para a Ciência e Tecnologia — Ministério da Educação e Ciência (FCT-MEC, Portugal) is acknowledged for funding (including fellowships SFRH/BD/42158/2007 to Marta M.B. Ribeiro, SFRH/BD/41750/2007 to Marco M. Domingues and SFRH/BD/70423/2010 to João M. Freire) and project PTDC/QUI-BIQ/119509/2010. Marie Curie Industry-Academia Partnerships and Pathways (European Commission) is also acknowledged for funding (FP7-PEOPLE-2007-3-1-IAPP, Project 230654)

Participação no XI Met-Encontro nacional sobre metodologias de laboratório.

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Evidence for a Massive Neutron Star from a Radial-Velocity Study of the Companion to the Black Widow Pulsar PSR B1957+20

The most massive neutron stars constrain the behavior of ultra-dense matter, with larger masses possible only for increasingly stiff equations of state. Here, we present evidence that the black widow pulsar, PSR B1957+20, has a high mass. We took spectra of its strongly irradiated companion and found an observed radial-velocity amplitude of K_obs=324+/-3 km/s. Correcting this for the fact that, due to the irradiation, the center of light lies inward relative to the center of mass, we infer a true radial-velocity amplitude of K_2=353+/-4 km/s and a mass ratio q=M_PSR/M_2=69.2+/-0.8. Combined with the inclination i=65+/-2 deg inferred from models of the lightcurve, our best-fit pulsar mass is M_PSR=2.40+/-0.12 M_sun. We discuss possible systematic uncertainties, in particular in the lightcurve modeling. Taking an upper limit of i<85 deg based on the absence of radio eclipses at high frequency, combined with a conservative lower-limit to the motion of the center of mass, K_2>343 km/s (q>67.3), we infer a lower limit to the pulsar mass of M_PSR>1.66 M_sun.Comment: 7 pages, 3 figures, 1 table, accepted for publication in ApJ; revision includes more detail on the spectral classification and discussion of other recent high neutron-star masse

Production, breeding and potential of cowpea crop in Brazil.

About 50 years ago cowpea was reported as a relatively minor tropical legume. However, in the last years, it has been emerging as one of the most important food legume of the 21st century (SINGH, 2010). Brazil is not out of this panorama. Brazilian agriculture is undergoing major technological changes and, in addition, globalization in agribusiness has caused impacts on the production chain of several crops, particularly those heavily dependent on the use of a large volume of agricultural inputs, mainly fertilizers and pesticides. Such crops have had a higher production cost each year. On the other hand, this situation has brought new opportunities. Business farmers have sought new alternatives for their production arrangements. In this context cowpea constitutes one of the best options.bitstream/item/85620/1/Doc-216-Production.pd

Aproveitamento do amendoim na dieta alimentar : BRS Havana

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Características químicas de sementes de melancia (Citrullus vulgaris), produzidas espontaneamente numa área cultivada com Atriplex irrigada com água salobra.

o objetivo de identificar e caracterizar as propriedades químicas de sementes de melancia - Citrullus vulgaris, (registro CPATSA n° 017, ano 1977), foram coletadas sementes ao acaso de um lote de frutos colhidos em 2004

Preparo de soluções.

Introdução; Cuidados gerais no preparo das soluções; Concentração de soluções; Diluição de soluções; Transformações mais usadas; exercicios sobre calculos de soluções;bitstream/CNPA-2009-09/14619/1/CIRTEC42.pd