Protective coating for salt-bath brazing

Ceramic coating, consisting of graphite, enameler's clay, and algin binder, applied to materials prior to salt bath brazing facilitates brazing process and results in superior joints. Alternate coating materials and their various proportions are given

Process for applying a protective coating for salt bath brazing Patent

Application techniques for protecting materials during salt bath brazin

On the componentwise linearity and the minimal free resolution of a tetrahedral curve

A tetrahedral curve is an unmixed, usually non-reduced, one-dimensional subscheme of projective 3-space whose homogeneous ideal is the intersection of powers of the ideals of the six coordinate lines. The second and third authors have shown that these curves have very nice combinatorial properties, and they have made a careful study of the even liaison classes of these curves. We build on this work by showing that they are "almost always" componentwise linear, i.e. their homogeneous ideals have the property that for any d, the degree d component of the ideal generates a new ideal whose minimal free resolution is linear. The one type of exception is clearly spelled out and studied as well. The main technique is a careful study of the way that basic double linkage behaves on tetrahedral curves, and the connection to the tetrahedral curves that are minimal in their even liaison classes. With this preparation, we also describe the minimal free resolution of a tetrahedral curve, and in particular we show that in any fixed even liaison class there are only finitely many tetrahedral curves with linear resolution. Finally, we begin the study of the generic initial ideal (gin) of a tetrahedral curve. We produce the gin for arithmetically Cohen-Macaulay tetrahedral curves and for minimal arithmetically Buchsbaum tetrahedral curves, and we show how to obtain it for any non-minimal tetrahedral curve in terms of the gin of the minimal curve in that even liaison class.Comment: 31 pages; v2 has very minor changes: fixed typos, added Remark 4.2 and char. zero hypothesis to 5.2, and reworded 5.5. To appear, J. Algebr

Proteolytic processing and activation of Clostridium perfringens epsilon toxin by caprine small intestinal contents.

Epsilon toxin (ETX), a pore-forming toxin produced by type B and D strains of Clostridium perfringens, mediates severe enterotoxemia in livestock and possibly plays a role in human disease. During enterotoxemia, the nearly inactive ETX prototoxin is produced in the intestines but then must be activated by proteolytic processing. The current study sought to examine ETX prototoxin processing and activation ex vivo using the intestinal contents of a goat, a natural host species for ETX-mediated disease. First, this study showed that the prototoxin has a KEIS N-terminal sequence with a molecular mass of 33,054 Da. When the activation of ETX prototoxin ex vivo by goat small intestinal contents was assessed by SDS-PAGE, the prototoxin was processed in a stepwise fashion into an ~27-kDa band or higher-molecular-mass material that could be toxin oligomers. Purified ETX corresponding to the ~27-kDa band was cytotoxic. When it was biochemically characterized by mass spectrometry, the copresence of three ETX species, each with different C-terminal residues, was identified in the purified ~27-kDa ETX preparation. Cytotoxicity of each of the three ETX species was then demonstrated using recombinant DNA approaches. Serine protease inhibitors blocked the initial proteotoxin processing, while carboxypeptidase inhibitors blocked further processing events. Taken together, this study provides important new insights indicating that, in the intestinal lumen, serine protease (including trypsin and possibly chymotrypsin) initiates the processing of the prototoxin but other proteases, including carboxypeptidases, then process the prototoxin into multiple active and stable species. Importance: Processing and activation by intestinal proteases is a prerequisite for ETX-induced toxicity. Previous studies had characterized the activation of ETX using only arbitrarily chosen amounts of purified trypsin and/or chymotrypsin. Therefore, the current study examined ETX activation ex vivo by natural host intestinal contents. These analyses demonstrated that (i) ETX processing in host intestinal contents occurs in an ordered, stepwise fashion, (ii) processing of prototoxin by host intestinal contents results in higher-molecular-mass material and 3 distinct ~27-kDa ETX species, and (iii) serine proteases, such as trypsin, chymotrypsin, and other proteases, including carboxypeptidases, play a role in the activation of ETX by intestinal contents. These studies provide new insights into the activation and processing of ETX and demonstrate that this process is more complicated than previously appreciated

Casimir force between eccentric cylinders

We consider the Casimir interaction between a cylinder and a hollow cylinder, both conducting, with parallel axis and slightly different radii. The Casimir force, which vanishes in the coaxial situation, is evaluated for both small and large eccentricities using the proximity approximation. The cylindrical configuration offers various experimental advantages with respect to the parallel planes or the plane-sphere geometries, leading to favourable conditions for the search of extra-gravitational forces in the micrometer range and for the observation of finite temperature corrections.Comment: To be published in Europhysics Letters. 7 pages, 4 figure