1,091 research outputs found
Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments
Unsynchronized cells of an essentially diploid strain of female Chinese hamster cells derived from lung tissue (CHL) were laser-UV-microirradiated (=257 nm) in the nucleus either at its central part or at its periphery. After 7–9 h postincubation with 0.5 mM caffeine, chromosome preparations were made in situ. Twenty-one and 29 metaphase spreads, respectively, with partial chromosome shattering (PCS) obtained after micro-irradiation at these two nuclear sites, were Q-banded and analyzed in detail. A positive correlation was observed between the frequency of damage of chromosomes and both their DNA content and length at metaphase. No significant difference was observed between the frequencies of damage obtained for individual chromosomes at either site of microirradiation. The frequency of joint damage of homologous chromosomes was low as compared to nonhomologous ones. Considerable variation was noted in different cells in the combinations of jointly shattered chromosomes. Evidence which justifies an interpretation of these data in terms of an interphase arrangement of chromosome territories is discussed. Our data strongly argue against somatic pairing as a regular event, and suggest a considerable variability of chromosome positions in different nuclei. However, present data do not exclude the possibility of certain non-random chromosomal arrangements in CHL-nuclei. The interphase chromosome distribution revealed by these experiments is compared with centromere-centromere, centromere-center and angle analyses of metaphase spreads and the relationship between interphase and metaphase arrangements of chromosomes is discussed
Intracranial V. cholerae Sialidase Protects against Excitotoxic Neurodegeneration
Converging evidence shows that GD3 ganglioside is a critical effector in a number of apoptotic pathways, and GM1 ganglioside has neuroprotective and noötropic properties. Targeted deletion of GD3 synthase (GD3S) eliminates GD3 and increases GM1 levels. Primary neurons from GD3S−/− mice are resistant to neurotoxicity induced by amyloid-β or hyperhomocysteinemia, and when GD3S is eliminated in the APP/PSEN1 double-transgenic model of Alzheimer's disease the plaque-associated oxidative stress and inflammatory response are absent. To date, no small-molecule inhibitor of GD3S exists. In the present study we used sialidase from Vibrio cholerae (VCS) to produce a brain ganglioside profile that approximates that of GD3S deletion. VCS hydrolyzes GD1a and complex b-series gangliosides to GM1, and the apoptogenic GD3 is degraded. VCS was infused by osmotic minipump into the dorsal third ventricle in mice over a 4-week period. Sensorimotor behaviors, anxiety, and cognition were unaffected in VCS-treated mice. To determine whether VCS was neuroprotective in vivo, we injected kainic acid on the 25th day of infusion to induce status epilepticus. Kainic acid induced a robust lesion of the CA3 hippocampal subfield in aCSF-treated controls. In contrast, all hippocampal regions in VCS-treated mice were largely intact. VCS did not protect against seizures. These results demonstrate that strategic degradation of complex gangliosides and GD3 can be used to achieve neuroprotection without adversely affecting behavior
Sigma-1 receptor agonist fluvoxamine for postoperative delirium in older adults: report of three cases
<p>Abstract</p> <p>Background</p> <p>Postoperative delirium is a topic of great importance in the geriatric surgical specialty. Although antipsychotic drugs are the medications most frequently used to treat this syndrome, these drugs are associated with a variety of adverse events, including sedation, extrapyramidal side effects, and cardiac arrhythmias. Drug treatment for postoperative delirium requires careful consideration of the balance between the effective management of symptoms and potential adverse effects.</p> <p>Methods</p> <p>We report on a Japanese woman (an 86-year-old (open reduction and internal fixation of the right femoral neck fracture), and two Japanese men (an 86-year-old (abdominal aortic aneurysm stent grafting), and a 77-year-old (right upper lobectomy due to lung tumour)) in which the selective serotonin reuptake inhibitor and sigma-1 receptor agonist fluvoxamine was effective in ameliorating the postoperative delirium of these patients.</p> <p>Results</p> <p>Delirium Rating Scale scores in these patients dramatically decreased after treatment with fluvoxamine.</p> <p>Conclusions</p> <p>Doctors should consider fluvoxamine as an alternative approach to treating postoperative delirium in older patients in order to avoid the risk of side effects and increased mortality by antipsychotic drugs.</p
RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis
Mucorales are an emerging group of human pathogens that are responsible for the lethal disease mucormycosis. Unfortunately, functional studies on the genetic factors behind the virulence of these organisms are hampered by their limited genetic tractability, since they are reluctant to classical genetic tools like transposable elements or gene mapping. Here, we describe an RNAi-based functional genomic platform that allows the identification of new virulence factors through a forward genetic approach firstly described in Mucorales. This platform contains a whole-genome collection of Mucor circinelloides silenced transformants that presented a broad assortment of phenotypes related to the main physiological processes in fungi, including virulence, hyphae morphology, mycelial and yeast growth, carotenogenesis and asexual sporulation. Selection of transformants with reduced virulence allowed the identification of mcplD, which encodes a Phospholipase D, and mcmyo5, encoding a probably essential cargo transporter of the Myosin V family, as required for a fully virulent phenotype of M. circinelloides. Knock-out mutants for those genes showed reduced virulence in both Galleria mellonella and Mus musculus models, probably due to a delayed germination and polarized growth within macrophages. This study provides a robust approach to study virulence in Mucorales and as a proof of concept identified new virulence determinants in M. circinelloides that could represent promising targets for future antifungal therapies
Research opportunities for argumentation in social networks
Nowadays, many websites allow social networking between their users in an
explicit or implicit way. In this work, we show how argumentation schemes theory can
provide a valuable help to formalize and structure on-line discussions and user opinions in
decision support and business oriented websites that held social networks between their users.
Two real case studies are studied and analysed. Then, guidelines to enhance social decision
support and recommendations with argumentation are provided.This work summarises results of the authors joint research, funded by an STMS of the Agreement Technologies COST Action 0801, by the Spanish government grants [CONSOLIDER-INGENIO 2010 CSD2007-00022, and TIN2012-36586-C03-01] and by the GVA project [PROMETEO 2008/051].Heras Barberá, SM.; Atkinson, KM.; Botti Navarro, VJ.; Grasso, F.; Julian Inglada, VJ.; Mcburney, PJ. (2013). Research opportunities for argumentation in social networks. Artificial Intelligence Review. 39(1):39-62. doi:10.1007/s10462-012-9389-0S3962391Amgoud L (2009) Argumentation for decision making. Argumentation in artificial intelligence. Springer, BerlinAnderson P (2007) What is Web 2.0? Ideas, technologies and implications for education. JISC Iechnology and Standards Watch reportBentahar J, Meyer CJJ, Moulin B (2007) Securing agent-oriented systems: an argumentation and reputation-based approach. In: Proceedings of the 4th international conference on information technology: new generations (ITNG 2007), IEEE Computer Society, pp 507–515Buckingham Shum S (2008) Cohere: towards Web 2.0 argumentation. In: Proceedings of the 2nd international conference on computational models of argument, COMMA, pp 28–30Burke R (2002) Hybrid recommender systems: survey and experiments. User Model User-Adapt Interact 12:331–370Cartwright D, Atkinson K (2008) Political engagement through tools for argumentation. In: Proceedings of the second international conference on computational models of argument (COMMA 2008), pp 116–127Chesñevar C, McGinnis J, Modgil S, Rahwan I, Reed C, Simari G, South M, Vreeswijk G, Willmott S (2006) Towards an argument interchange format. Knowl Eng Rev 21(4):293–316Chesñevar CI, Maguitman AG, Gonzà lez MP (2009) Empowering recommendation technologies through argumentation. Argumentation in artificial intelligence. Springer, Berlin, pp 403–422GarcÃa AJ, Dix J, Simari GR (2009) Argument-based logic programming. Argumentation in artificial intelligence. Springer, BerlinGolbeck J (2006) Generating predictive movie recommendations from trust in social networks. In: Proceedings of the fourth international conference on trust management, LNCS, vol 3986, 93–104Gordon T, Prakken H, Walton D (2007) The Carneades model of argument and burden of proof. Artif Intell 171(10–15):875–896Guha R, Kumar R, Raghavan P, Tomkins A (2004) Propagating trust and distrust. In: Proceedings of the 13th international conference on, World Wide Web, pp 403–412Heras S, Navarro M, Botti V, Julián V (2009) Applying dialogue games to manage recommendation in social networks. In: Proceedings of the 6th international workshop on argumentation in multi-agent aystems, ArgMASHeras S, Atkinson K, Botti V, Grasso F, Julián V, McBurney P (2010a) How argumentation can enhance dialogues in social networks. In: Proceedings of the 3rd international conference on computational models of argument, COMMA, vol 216, pp 267–274Heras S, Atkinson K, Botti V, Grasso F, Julián V, McBurney P (2010b) Applying argumentation to enhance dialogues in social networks. In: ECAI 2010 workshop on computational models of natural argument, CMNA, pp 10–17Karacapilidis N, Tzagarakis M (2007) Web-based collaboration and decision making support: a multi-disciplinary approach. Web-Based Learn Teach Technol 2(4):12–23Kim D, Benbasat I (2003) Trust-related arguments in internet stores: a framework for evaluation. J Electron Commer Res 4(2):49–64Kim D, Benbasat I (2006) The effects of trust-assuring arguments on consumer trust in internet stores: application of Toulmin’s model of argumentation. Inf Syst Rese 17(3):286–300Laera L, Tamma V, Euzenat J, Bench-Capon T, Payne T (2006) Reaching agreement over ontology alignments. In: Proceedings of the 5th international semantic web conference (ISWC 2006)Lange C, Bojãrs U, Groza T, Breslin J, Handschuh S (2008) Expressing argumentative discussions in social media sites. In: Social data on the web (SDoW2008) workshop at the 7th international semantic web conferenceLinden G, Smith B, York J (2003) Amazon.com recommendations: item-to-item collaborative filtering. IEEE Internet Comput 7(1):76–80Linden G, Hong J, Stonebraker M, Guzdial M (2009) Recommendation algorithms, online privacy and more. Commun ACM, 52(5)Mika P (2007) Ontologies are us: a unified model of social networks and semantics. J Web Semant 5(1):5–15Montaner M, López B, de la Rosa JL (2002) Opinion-based filtering through trust. In: Cooperative information agents VI, LNCS, vol 2446, pp 127–144Ontañón S, Plaza E (2008) Argumentation-based information exchange in prediction markets. In: Proceedings of the 5th international workshop on argumentation in multi-agent systems, ArgMASPazzani MJ, Billsus D (2007) Content-based recommendation systems. In: The adaptive web, LNCS, vol 4321, pp 325–341Rahwan I, Zablith F, Reed C (2007) Laying the foundations for a world wide argument web. Artif Intell 171(10–15):897–921Rahwan I, Banihashemi B (2008) Arguments in OWL: a progress report. In: Proceedings of the 2nd international conference on computational models of argument (COMMA), pp 297–310Reed C, Walton D (2007) Argumentation schemes in dialogue. In: Dissensus and the search for common ground, OSSA-07, volume CD-ROM, pp 1–11Sabater J, Sierra C (2002) Reputation and social network analysis in multi-agent systems. In: Proceedings of the 1st international joint conference on autonomous agents and multiagent systems, vol 1, pp 475–482Schafer JB, Konstan JA, Riedl J (2001) E-commerce recommendation applications. Data Min Knowl Discov 5:115–153Schafer JB, Frankowski D, Herlocker J, Sen S (2007) Collaborative filtering recommender systems. In: The adaptive web, LNCS, vol 4321, pp 291–324Schneider J, Groza T, Passant A (2012) A review of argumentation for the aocial semantic web. Semantic web-interoperability, usability, applicability. IOS Press, Washington, DCTempich C, Pinto HS, Sure Y, Staab S (2005) An argumentation ontology for distributed, loosely-controlled and evolvInG Engineering processes of oNTologies (DILIGENT). In: Proceedings of the 2nd European semantic web conference, ESWC, pp 241–256Toulmin SE (1958) The uses of argument. Cambridge University Press, Cambridge, UKTrojahn C, Quaresma P, Vieira R, Isaac A (2009) Comparing argumentation frameworks for composite ontology matching. in: Proceedings of the 6th international workshop on argumentation in multi-agent systems, ArgMASTruthMapping. http://truthmapping.com/Walter FE, Battiston S, Schweitzer F (2007) A model of a trust-based recommendation system on a social network. J Auton Agents Multi-Agent Syst 16(1):57–74Walton D, Krabbe E (1995) Commitment in dialogue: basic concepts of interpersonal reasoning. State University of New York Press, New York, NYWalton D, Reed C, Macagno F (2008) Argumentation schemes. Cambridge University Press, CambridgeWells S, Gourlay C, Reed C (2009) Argument blogging. Computational models of natural argument, CMNAWyner A, Schneider J (2012) Arguing from a point of view. In: Proceedings of the first international conference on agreement technologie
Knowledge, attitude, and behavior in patients with atrial fibrillation undergoing radiofrequency catheter ablation
Fitness components and natural selection: why are there different patterns on the emergence of drug resistance in Plasmodium falciparum and Plasmodium vivax?
The long-term fate of the hip arthrodesis: does it remain a valid procedure for selected cases in the 21st century?
Even in current orthopaedic practice, some cases are still not suitable candidates for hip replacement and hip fusion remains the only option in these highly selected patients. In this retrospective study we describe the long-term clinical outcome, quality of life and radiological evaluation of all adjacent joints in a cohort of 47 hip fusions. The main objective of our study was to show the long-term effects of a fusion. Thirty patients were analysed after an average of 18.2 years (range 6.2–30.5 years) with a mean SMFA of 31.2 (range 9–70). The VAS for pain for the fused hip was an average 1.9 (range 0–8), for the contralateral hip 2.0 (0–8), for the ipsilateral knee 2.0 (0–8), for the contralateral knee 1.8 (0–8) and for the lower back 3.6 (0–8). Average walking distance was 115 minutes (range 10–unlimited). Although the hip arthrodesis has lost popularity, it still is an option for the young patient with severe hip disorders, while leaving the possibility to perform a THA at a later stage. If the arthrodesis is performed with an optimal alignment of the leg, complaints from the adjacent joints are minimal, even in the long-term, and an acceptable quality of life can be obtained. We believe that in highly selected cases a hip fusion, even in current practice, is still a valid option
Further investigation of confirmed urinary tract infection (UTI) in children under five years: a systematic review.
Background: Further investigation of confirmed UTI in children aims to prevent renal scarring and future complications. Methods: We conducted a systematic review to determine the most effective approach to the further investigation of confirmed urinary tract infection (UTI) in children under five years of age. Results: 73 studies were included. Many studies had methodological limitations or were poorly reported. Effectiveness of further investigations: One study found that routine imaging did not lead to a reduction in recurrent UTIs or renal scarring. Diagnostic accuracy: The studies do not support the use of less invasive tests such as ultrasound as an alternative to renal scintigraphy, either to rule out infection of the upper urinary tract (LR- = 0.57, 95%CI: 0.47, 0.68) and thus to exclude patients from further investigation or to detect renal scarring (LR+ = 3.5, 95% CI: 2.5, 4.8). None of the tests investigated can accurately predict the development of renal scarring. The available evidence supports the consideration of contrast-enhanced ultrasound techniques for detecting vesico-ureteric reflux (VUR), as an alternative to micturating cystourethrography (MCUG) (LR+ = 14.1, 95% CI: 9.5, 20.8; LR- = 0.20, 95%CI: 0.13, 0.29); these techniques have the advantage of not requiring exposure to ionising radiation. Conclusion: There is no evidence to support the clinical effectiveness of routine investigation of children with confirmed UTI. Primary research on the effectiveness, in terms of improved patient outcome, of testing at all stages in the investigation of confirmed urinary tract infection is urgently required
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