5,999 research outputs found
Genome-Wide Transcriptional Changes and Lipid Profile Modifications Induced by Medicago truncatula N5 Overexpression at an Early Stage of the Symbiotic Interaction with Sinorhizobium meliloti.
Plant lipid-transfer proteins (LTPs) are small basic secreted proteins, which are characterized by lipid-binding capacity and are putatively involved in lipid trafficking. LTPs play a role in several biological processes, including the root nodule symbiosis. The Medicago truncatula nodulin 5 (MtN5) LTP has been proved to positively regulate the nodulation capacity, controlling rhizobial infection and nodule primordia invasion. To better define the lipid transfer protein MtN5 function during the symbiosis, we produced MtN5-downregulated and -overexpressing plants, and we analysed the transcriptomic changes occurring in the roots at an early stage of Sinorhizobium meliloti infection. We also carried out the lipid profile analysis of wild type (WT) and MtN5-overexpressing roots after rhizobia infection. The downregulation of MtN5 increased the root hair curling, an early event of rhizobia infection, and concomitantly induced changes in the expression of defence-related genes. On the other hand, MtN5 overexpression favoured the invasion of the nodules by rhizobia and determined in the roots the modulation of genes that are involved in lipid transport and metabolism as well as an increased content of lipids, especially galactolipids that characterize the symbiosome membranes. Our findings suggest the potential participation of LTPs in the synthesis and rearrangement of membranes occurring during the formation of the infection threads and the symbiosome membrane
Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana
In this work, we investigated the molecular basis of autotrophic vs. mixotrophic growth of Chlorella sorokiniana, one of the most productive microalgae species with high potential to produce biofuels, food and high value compounds. To increase biomass accumulation, photosynthetic microalgae are commonly cultivated in mixotrophic conditions, adding reduced carbon sources to the growth media. In the case of C. sorokiniana, the presence of acetate enhanced biomass, proteins, lipids and starch productivity when compared to autotrophic conditions. Despite decreased chlorophyll content, photosynthetic properties were essentially unaffected while differential gene expression profile revealed transcriptional regulation of several genes mainly involved in control of carbon flux. Interestingly, acetate assimilation caused upregulation of phosphoenolpyruvate carboxylase enzyme, enabling potential recovery of carbon atoms lost by acetate oxidation. The obtained results allowed to associate the increased productivity observed in mixotrophy in C. sorokiniana with a different gene regulation leading to a fine regulation of cell metabolism
Gas Analysis and Monitoring Systems for the RPC Detector of CMS at LHC
The Resistive Plate Chambers (RPC) detector of the CMS experiment at the LHC
proton collider (CERN, Switzerland) will employ an online gas analysis and
monitoring system of the freon-based gas mixture used. We give an overview of
the CMS RPC gas system, describe the project parameters and first results on
gas-chromatograph analysis. Finally, we report on preliminary results for a set
of monitor RPC.Comment: 9 pages, 8 figures. Presented by Stefano Bianco (Laboratori Nazionali
di Frascati dell'INFN) at the IEEE NSS, San Diego (USA), October 200
Recent results and developments on double-gap RPCs for CMS
Abstract A 3 mm wide-gap Resistive Plate Chamber, as proposed for CMS, has been tested in the H2 Cern beam line. Results on efficiency, rate capability, time resolution and cluster size are reported
Resistive Plate Chambers in avalanche mode: a comparison between model predictions and experimental results
Abstract In this paper a model simulating the main aspects of avalanche growth and signal development in Resistive Plate Chambers (RPCs) is presented. The model has been used to compute the performances, in particular, charge distribution and efficiency of single- double- and multi-gap RPCs, and to compare them with the available experimental results. This model could be used to optimize the characteristics of this type of detector with a view to its use in the future large experiments at LHC: ATLAS and CMS
The CMS RPC gas gain monitoring system: an overview and preliminary results
The status of the CMS RPC Gas Gain Monitoring (GGM) system developed at the
Frascati Laboratory of INFN (Istituto Nazionale di Fisica Nucleare) is reported
on. The GGM system is a cosmic ray telescope based on small RPC detectors
operated with the same gas mixture used by the CMS RPC system. The GGM gain and
efficiency are continuously monitored on-line, thus providing a fast and
accurate determination of any shift in working point conditions. The
construction details and the first result of GGM commissioning are described.Comment: 8 pages, 9 figures, uses lnfprepCMS.sty, presented by L. Benussi at
RPC07, Mumbai, INDIA 200
The Upgrade of the CMS RPC System during the First LHC Long Shutdown
The CMS muon system includes in both the barrel and endcap region Resistive
Plate Chambers (RPC). They mainly serve as trigger detectors and also improve
the reconstruction of muon parameters. Over the years, the instantaneous
luminosity of the Large Hadron Collider gradually increases. During the LHC
Phase 1 (~first 10 years of operation) an ultimate luminosity is expected above
its design value of 10^34/cm^2/s at 14 TeV. To prepare the machine and also the
experiments for this, two long shutdown periods are scheduled for 2013-2014 and
2018-2019. The CMS Collaboration is planning several detector upgrades during
these long shutdowns. In particular, the muon detection system should be able
to maintain a low-pT threshold for an efficient Level-1 Muon Trigger at high
particle rates. One of the measures to ensure this, is to extend the present
RPC system with the addition of a 4th layer in both endcap regions. During the
first long shutdown, these two new stations will be equipped in the region
|eta|<1.6 with 144 High Pressure Laminate (HPL) double-gap RPCs operating in
avalanche mode, with a similar design as the existing CMS endcap chambers.
Here, we present the upgrade plans for the CMS RPC system for the fist long
shutdown, including trigger simulation studies for the extended system, and
details on the new HPL production, the chamber assembly and the quality control
procedures.Comment: 9 pages, 6 figures, presented by M.Tytgat at the XI workshop on
Resistive Plate Chambers and Related Detectors (RPC2012), INFN - Laboratori
Nazionali di Frascati, February 5-10, 201
Draft genome assembly and transcriptome data of the icefish Chionodraco myersi reveal the key role of mitochondria for a life without hemoglobin at subzero temperatures
Antarctic fish belonging to Notothenioidei represent an extraordinary example of radiation in
the cold. In addition to the absence of hemoglobin, icefish show a number of other striking
peculiarities including large-diameter blood vessels, high vascular densities, mitochondriarich
muscle cells, and unusual mitochondrial architecture. In order to investigate the bases of
icefish adaptation to the extreme Southern Ocean conditions we sequenced the complete
genome of the icefish Chionodraco myersi. Comparative analyses of the icefish genome with
those of other teleost species, including two additional white-blooded and five red-blooded
notothenioids, provided a new perspective on the evolutionary loss of globin genes. Muscle
transcriptome comparative analyses against red-blooded notothenioids as well as temperate
fish revealed the peculiar regulation of genes involved in mitochondrial function in icefish.
Gene duplication and promoter sequence divergence were identified as genome-wide patterns
that likely contributed to the broad transcriptional program underlying the unique
features of icefish mitochondria
Simulation of the CMS Resistive Plate Chambers
The Resistive Plate Chamber (RPC) muon subsystem contributes significantly to
the formation of the trigger decision and reconstruction of the muon trajectory
parameters. Simulation of the RPC response is a crucial part of the entire CMS
Monte Carlo software and directly influences the final physical results. An
algorithm based on the parametrization of RPC efficiency, noise, cluster size
and timing for every strip has been developed. Experimental data obtained from
cosmic and proton-proton collisions at TeV have been used for
determination of the parameters. A dedicated validation procedure has been
developed. A good agreement between the simulated and experimental data has
been achieved.Comment: to be published in JINS
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