Characterization of visual object representations in rat primary visual cortex

For most animal species, quick and reliable identification of visual objects is critical for survival. This applies also to rodents, which, in recent years, have become increasingly popular models of visual functions. For this reason in this work we analyzed how various properties of visual objects are represented in rat primary visual cortex (V1). The analysis has been carried out through supervised (classification) and unsupervised (clustering) learning methods. We assessed quantitatively the discrimination capabilities of V1 neurons by demonstrating how photometric properties (luminosity and object position in the scene) can be derived directly from the neuronal responses

Probiotic-Derived Polyphosphate Enhances the Epithelial Barrier Function and Maintains Intestinal Homeostasis through Integrin–p38 MAPK Pathway

Probiotics exhibit beneficial effects on human health, particularly in the maintenance of intestinal homeostasis in a complex manner notwithstanding the diversity of an intestinal flora between individuals. Thus, it is highly probable that some common molecules secreted by probiotic and/or commensal bacteria contribute to the maintenance of intestinal homeostasis and protect the intestinal epithelium from injurious stimuli. To address this question, we aimed to isolate the cytoprotective compound from a lactobacillus strain, Lactobacillus brevis SBC8803 which possess the ability to induce cytoprotective heat shock proteins in mouse small intestine. L. brevis was incubated in MRS broth and the supernatant was passed through with a 0.2-µm filter. Caco2/bbe cells were treated with the culture supernatant, and HSP27 expression was evaluated by Western blotting. HSP27-inducible components were separated by ammonium sulfate precipitation, DEAE anion exchange chromatography, gel filtration, and HPLC. Finally, we identified that the HSP27-inducible fraction was polyphosphate (poly P), a simple repeated structure of phosphates, which is a common product of lactobacilli and other bacteria associated with intestinal microflora without any definitive physiological functions. Then, poly P was synthesized by poly P-synthesizing enzyme polyphosphate kinase. The synthesized poly P significantly induced HSP27 from Caco2/BBE cells. In addition, Poly P suppressed the oxidant-induced intestinal permeability in the mouse small intestine and pharmacological inhibitors of p38 MAPK and integrins counteract its protective effect. Daily intrarectal administration of poly P (10 µg) improved the inflammation grade and survival rate in 4% sodium dextran sulfate-administered mice. This study, for the first time, demonstrated that poly P is the molecule responsible for maintaining intestinal barrier actions which are mediated through the intestinal integrin β1-p38 MAPK

Salmonella Type III Effector AvrA Stabilizes Cell Tight Junctions to Inhibit Inflammation in Intestinal Epithelial Cells

Salmonella Typhimurium is a major cause of human gastroenteritis. The Salmonella type III secretory system secretes virulence proteins, called effectors. Effectors are responsible for the alteration of tight junction (TJ) structure and function in intestinal epithelial cells. AvrA is a newly described bacterial effector found in Salmonella. We report here that AvrA expression stabilizes cell permeability and tight junctions in intestinal epithelial cells. Cells colonized with an AvrA-deficient bacterial strain (AvrA−) displayed decreased cell permeability, disruption of TJs, and an increased inflammatory response. Western blot data showed that TJ proteins, such as ZO-1, claudin-1, decreased after AvrA- colonization for only 1 hour. In contrast, cells colonized with AvrA-sufficient bacteria maintained cell permeability with stabilized TJ structure. This difference was confirmed in vivo. Fluorescent tracer studies showed increased fluorescence in the blood of mice infected with AvrA- compared to those infected with the AvrA-sufficient strains. AvrA- disrupted TJ structure and function and increased inflammation in vivo, compared to the AvrA- sufficient strain. Additionally, AvrA overexpression increased TJ protein expression when transfected into colonic epithelial cells. An intriguing aspect of this study is that AvrA stabilized TJs, even though the other TTSS proteins, SopB, SopE, and SopE2, are known to disrupt TJs. AvrA may play a role in stabilizing TJs and balancing the opposing action of other bacterial effectors. Our findings indicate an important role for the bacterial effector AvrA in regulation of intestinal epithelial cell TJs during inflammation. The role of AvrA represents a highly refined bacterial strategy that helps the bacteria survive in the host and dampen the inflammatory response

A review of friction models in interacting joints for durability design.

This paper presents a comprehensive review of friction modelling to provide an understanding of design for durability within interacting systems. Friction is a complex phenomenon and occurs at the interface of two components in relative motion. Over the last several decades, the effects of friction and its modelling techniques have been of significant interests in terms of industrial applications. There is however a need to develop a unified mathematical model for friction to inform design for durability within the context of varying operational conditions. Classical dynamic mechanisms model for the design of control systems has not incorporated friction phenomena due to non-linearity behaviour. Therefore, the tribological performance concurrently with the joint dynamics of a manipulator joint applied in hazardous environments needs to be fully analysed. Previously the dynamics and impact models used in mechanical joints with clearance have also been examined. The inclusion of reliability and durability during the design phase is very important for manipulators which are deployed in harsh environmental and operational conditions. The revolute joint is susceptible to failures such as in heavy manipulators these revolute joints can be represented by lubricated conformal sliding surfaces. The presence of pollutants such as debris and corrosive constituents has the potential to alter the contacting surfaces, would in turn affect the performance of revolute joints, and puts both reliability and durability of the systems at greater risks of failure. Key literature is identified and a review on the latest developments of the science of friction modelling is presented here. This review is based on a large volume of knowledge. Gaps in the relevant field have been identified to capitalise on for future developments. Therefore, this review will bring significant benefits to researchers, academics and industrial professionals

Shape similarity, better than semantic membership, accounts for the structure of visual object representations in a population of monkey inferotemporal neurons

The anterior inferotemporal cortex (IT) is the highest stage along the hierarchy of visual areas that, in primates, processes visual objects. Although several lines of evidence suggest that IT primarily represents visual shape information, some recent studies have argued that neuronal ensembles in IT code the semantic membership of visual objects (i.e., represent conceptual classes such as animate and inanimate objects). In this study, we investigated to what extent semantic, rather than purely visual information, is represented in IT by performing a multivariate analysis of IT responses to a set of visual objects. By relying on a variety of machine-learning approaches (including a cutting-edge clustering algorithm that has been recently developed in the domain of statistical physics), we found that, in most instances, IT representation of visual objects is accounted for by their similarity at the level of shape or, more surprisingly, low-level visual properties. Only in a few cases we observed IT representations of semantic classes that were not explainable by the visual similarity of their members. Overall, these findings reassert the primary function of IT as a conveyor of explicit visual shape information, and reveal that low-level visual properties are represented in IT to a greater extent than previously appreciated. In addition, our work demonstrates how combining a variety of state-of-the-art multivariate approaches, and carefully estimating the contribution of shape similarity to the representation of object categories, can substantially advance our understanding of neuronal coding of visual objects in cortex

Ranking Cooperative Industries According to Development Indices Affecting Cooperative Share Increase in the GDP

Creating structural changes for efficiency improvement in the economy of cooperative sector requires a comprehensive study, using a quick and focused approach. This will substitute more efficient strategies for former policies in line with growth and development of cooperative sector. Cooperative industries are of the most important areas of activity in the cooperative sector. In order to have a clear view of growth in this sector it is necessary to rank cooperative industries according to their efficiency. In this study, statistical methods such as numerical taxonomy and factor analysis have been used to rank cooperative industries on the basis of macro-economic variables including per capita employment, investment production and exports. Ranking cooperative industries showed that the food and beverage manufacturing industry has the first degree priority because of the cheap and unskilled labor as well as its inputs which is securable domestically. This means that paying attention to cooperative food and beverage industry would be most effective in terms of the sector's development

Investigating Formation Flying and COTS in an Integrated Simulation Environment

The Canadian Space Agency’s (CSA) Software and Ground Segment (SGS) section has the mandate to develop innovative software and ground segment technologies. The implementation of formation flying concepts for Canadian missions is also currently under investigation at CSA's Spacecraft Engineering section. To that end, there is an ongoing development of a simulation environment to test COTS (Commercial-Off-The-Shelf) and formation flying technologies. Some of today's spacecraft are laboriously custom designed for a specific mission and a limited set of tasks. Development time can be lengthy (several years), which means that designs do not take advantage of the most recent technology. Designs also tend to be extremely inflexible, creating a spacecraft that cannot be easily adapted to future missions. A design ethic that promotes reusability is much more cost-effective and increases the time available to advance new technologies. COTS offer advantages such as a reduced development time, an increased product selection, faster and cheaper parts replacement, and extensively tested advanced designs. The main drawbacks to COTS use in space are susceptibility to radiation and in some cases decreased reliability. Since one of the main advantages of formation flying is the reduced mission sensitivity to a spacecraft failure, the risk associated with COTS, which has hindered its use in conventional space missions, is less concerning in the context of a multiple spacecraft mission. Achieving some level of standardization is a problem currently confronting the space industry, which must be addressed to realize the cost savings that can come from mass production and spacecraft interoperability. The use of standard components with standard interfaces also reduces development time. As well, since part of the goal is to have spacecraft already in orbit regroup and possibly join with new spacecraft to accomplish other missions, some forwards and backwards compatibility between generations of spacecraft will be necessary. This paper describes an integrated simulation environment that uses COTS spacecraft and simulation components to investigate formation flying scenarios and their benefits and challenges. A few of the well-known industry software and hardware tools incorporated into the environment include Analytical Graphic's STK, Mathwork's Matlab/Simulink, CAE Electronic's Real-time Object-Oriented Simulation Environment (ROSETM), Intel's StrongARM processor, and the PC-104 architecture for rugged embedded systems. There are short term plans to incorporate goal decomposition hierarchies to implement autonomous operations and robust fault tolerance. This will be implemented on high-speed logic based controller cards, known as Q4 cards, developed by Xiphos Technologies Inc. The simulation environment was developed to allow spacecraft designers or mission operators to test their respective technologies or ideas in a modular structure rapidly, accurately, and cheaply. This will enhance the successful use of formation flying spacecraft and improve the technologies needed to make formation flying a feasible and a cost effective reality