Revision of the taxonomic position of Pseudocypretta Klie, 1932 (Ostracoda: Cyprididae), with a redescription of P. maculata Klie, 1932

Pseudocypretta maculataKlie, 1932, type species of the genus, is redescribed based on new material from Thailand. The main diagnostic features of the species are the presence of marginal septa, presence of Wouters organ on the first antenna, strongly serrated claw G2 of the second antenna (A2), small β seta on the mandibular palp, elongated terminal segment of the maxillular palp, smooth and large bristles on the third endite of the maxillula, undivided penultimate segment of the second thoracopod (T2), absence of d1 seta on the T2, completely separated terminal segment of the third thoracopod, absence of a pincer organ, and reduced caudal ramus with a triangular base. The taxonomic position of PseudocyprettaKlie, 1932 is also revised. Based on molecular analysis using the cytochrome c oxidase subunit I (COI), Pseudocypretta appears closer to Cypridopsinae Kaufmann, 1900 than to Cyprettinae Hartmann, 1971. This result is congruent with morphological evidence and, among other cypridopsine genera, Pseudocypretta shows highest similarity with CyprettadopsisSavatenalinton, 2020 given its reduced caudal ramus, the strongly serrated claw G2 of the A2, absence of pincer organ, and presence of marginal septa. Pseudocypretta is therefore allocated to the tribe Cyprettadopsini Savatenalinton, 2020

Monitoring the impact of fertilizers on soil leachates using sequential injection analysis for multiparametric determination

info:eu-repo/semantics/publishedVersio

In silico and in vivo analysis reveal a novel gene in Saccharomyces cerevisiae trehalose metabolism

BACKGROUND: The ability to respond rapidly to fluctuations in environmental changes is decisive for cell survival. Under these conditions trehalose has an essential protective function and its concentration increases in response to enhanced expression of trehalose synthase genes, TPS1, TPS2, TPS3 and TSL1. Intriguingly, the NTH1 gene, which encodes neutral trehalase, is highly expressed at the same time. We have previously shown that trehalase remains in its inactive non-phosphorylated form by the action of an endogenous inhibitor. Recently, a comprehensive two-hybrid analysis revealed a 41-kDa protein encoded by the YLR270w ORF, which interacts with NTH1p. RESULTS: In this work we investigate the correlation of this Trehalase Associated Protein, in trehalase activity regulation. The neutral trehalase activity in the ylr270w mutant strain was about 4-fold higher than in the control strain. After in vitro activation by PKA the ylr270w mutant total trehalase activity increased 3-fold when compared to a control strain. The expression of the NTH1 gene promoter fused to the heterologous reporter lacZ gene was evaluated. The mutant strain lacking YLR270w exhibited a 2-fold increase in the NTH1-lacZ basal expression when compared to the wild type strain. CONCLUSIONS: These results strongly indicate a central role for Ylr270p in inhibiting trehalase activity, as well as in the regulation of its expression preventing a wasteful futile cycle of synthesis-degradation of trehalose

Análise estrutural de aduela prefabricada em betão reforçado com fibras para o revestimento de túneis

A presente comunicação descreve a investigação desenvolvida no âmbito da análise estrutural de aduelas prefabricadas em betão reforçado com fibras (BRFA), com comportamento melhorado ao fogo, para os casos de carga a que estão sujeitas durante a sua vida útil. Os ensaios necessários à caracterização das propriedades do BRFA desenvolvido são descritos e os resultados obtidos são apresentados

Biparametric sequential injection system with on-line solid phase extraction for the determination of copper and zinc in waters

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Qualidade sensorial da carne de cordeiros Morada Nova com ou sem suplementação, abatidos em diferentes idades.

Resumo: Objetivou-se caracterizar a carne de cordeiros Morada Nova abatidos em diferentes idades e submetidos ou não a suplementação. Foram utilizados 32 cordeiros, machos, não castrados, abatidos aos 60, 78, 106 e 134 dias, com grupo de 8 cordeiros em cada abate, num esquema fatorial 4x2. Avaliaram-se os parâmetros de dureza, suculência, sabor, cor, aroma e aceitação global, para tanto foi utilizado um painel treinado com 13 pessoas. A suplementação não modificou as características sensoriais, enquanto a idade ao abate não altera dureza, suculência e aceitação global. O sabor é intensificado com o aumento da idade de abate e a cor diminui com o aumento da idade, mas só foi diferente mesmo aos 60 e 134 dias, os outros são iguais. O aroma aos 60 dias é menos intenso que o restante. [Sensory quality of meat from lambs Morada Nova with or without supplementation, slaughtered in different ages]. Abstract: bjective of characterizing the meat from lambs Morada Nova slaughtered in different ages and submitted or no supplementation. We used 32 uncastrated males, lambs slaughtered at 60, 78, 106 and 134 days, with a group of 8 lambs in each slaughter, a factorial 4 x 2. We evaluated the parameters of hardness, juiciness, taste, colour, aroma and global acceptance, for both used a trained panel with 13 people. No supplementation modified sensory characteristics, while the age at slaughter does not change hardness, juiciness and global acceptance. The flavor is intensified with increasing age of slaughter and the color decreases with increasing age, but was only really different to 60 and 134 days, others are equal. The aroma to 60 days is less intense than the rest