O programa nacional de fortalecimento da agricultura familiar no Brasil: uma análise sobre a distribuição regional e setorial dos recursos.

No Brasil, as políticas públicas para o espaço rural sempre tenderam a priorizar a agricultura patronal, em detrimento dos agricultores familiares. Todavia, os estudos realizados pelos órgãos FAO - INCRA deram subsídio para a criação do Programa Nacional de Fortalecimento da Agricultura Familiar (PRONAF), resultando em um novo direcionamento dos investimentos públicos, os quais passaram a contemplar o segmento dos agricultores familiares. Entende-se o PRONAF como uma política não-compensatória, que, apesar de seus problemas, tem contribuído de fato para mudanças e melhorias no espaço agrário brasileiro. Desde sua criação no final da década de 1990, o PRONAF passou por várias mudanças em sua estrutura administrativa e operacional, a fim de alcançar seus objetivos e adequar-se face a complexa realidade social agrária brasileira. Sendo assim, o presente estudo visa discutir as ações do Estado por meio desse Programa, a partir de suas linhas de atuação, bem como analisar a distribuição de suas concessões de crédito regional e setorialmente. Assim, os procedimentos metodológicos utilizados para a realização deste trabalho compreendem pesquisa bibliográfica e documental, além de pesquisa em fontes secundárias, no intuito de obter dados e informações relevantes para a análise das relações sociais estabelecidas em meio a esse processo de concretização e espacialização desse Programa. Dentre as implicações do PRONAF pode-se notar em âmbito nacional, uma diminuição da disparidade regional brasileira, bem como a preocupação que o Programa tem demonstrado com os aspectos socioculturais locais e regionais, como forma de garantir que seus investimentos perpassem a dimensão econômica, mas valorize outras dimensões, a exemplo dos elementos culturais

Geotechnical Performance of a Tunnel in Soft Ground

The analysis of a tunnel section excavated through soft ground is presented. The tunnel construction was monitored and its behavior is compared with results obtained by a numerical simulation. The field instrumentation and the geotechnical properties of the site are presented and the modeling of the soil behavior using Lade\u27s model discussed

Tecnologia de aplicação de defensivos agrícolas.

Defensivos agrícolas. Alvo biológico e químico. Formulação dos defensivos agrícolas. Vias de intoxicação. Métodos de aplicação de defensivos agrícolas. Fatores fundamentais em uma aplicação de defensivos. Equipamentos de proteção individual (E. P. I.). Preparo e aplicação da calda. Fatores que influenciam nas pulverizações. Regulagem e calibração de um pulverizador de barras. Perdas por deriva. Perdas por evaporação. Manutenção de pulverizadores. Primeiros socorros. Avanços no desenvolvimento de novos equipamentos. Considerações finais.bitstream/CNPAT-2010/10340/1/Dc-102.pd

Estratégias para o estabelecimento e manutenção de áreas livres e de baixa prevalência de moscas-das-frutas.

Determinação de uma área livre de Moscas-das-Frutas (ALMF); Metas do programa; Procedimentos para a implantação de uma ALMF; Escolha da éspecie-alvo; Seleção dos Municípios e cadastro dos produtores da região; Delimitação da faixa de proteção permanente (Área Tampão); Estrutura organizacional; Monitoramento populacional da praga; Atividades de campo; Interpretação dos dados de captura; Plano de contigência; Preparação e envio de relatórios; Criação de decreto estadual; Estabelecimento de barreiras zoofitossanitárias; Procedimentos para a manutenção de uma área livre de pragas; Medidas fitossanitárias.bitstream/CNPAT-2010/9619/1/Ci-022.pd

CP tests of Higgs couplings in t¯th semileptonic events at the LHC

The CP nature of the Higgs coupling to top quarks is addressed in this paper, in single charged lepton final states of t¯th events produced in proton-proton collisions at the LHC. Pure scalar (h=H) and pseudoscalar (h=A) Higgs boson signal events, generated with MadGraph5_aMC@NLO, are fully reconstructed using a kinematic fit. Angular distributions of the decay products, as well as CP-sensitive asymmetries, are exploited to separate and gain sensitivity to possible pseudoscalar components of the Higgs boson and reduce the contribution from the dominant irreducible background t¯tb¯b. Significant differences are found between the pure CP-even and -odd signal hypotheses as well as with respect to the Standard Model background, in particular the t¯tb¯b contribution. Such differences survive the event reconstruction, allowing one to define optimal observables to extract the Higgs couplings parameters from a global fit. A dedicated analysis is applied to efficiently identify signal events and reject as much as possible the expected Standard Model background. The results obtained are compared with a similar analysis in the dilepton channel. We show that the single lepton channel is more promising overall and can be used in combination to study the CP nature of the Higgs coupling to top quarks.We would like to thank S. Amor Dos Santos et al. for providing the angular distributions from their analysis in the dileptonic channel [17,18]. This work was partially supported by Fundacao para a Ciencia e Tecnologia (Projects No. CERN/FIS-NUC/0005/2015 and No. CERN/FP/123619/2011, Grant No. SFRH/BPD/100379/2014, and Contract No. IF/01589/2012/CP0180/CT0002). Special thanks goes to our long-term collaborator Filipe Veloso for the invaluable help and availability on the evaluation of the confidence limits discussed in this paper.info:eu-repo/semantics/publishedVersio

Acceptance and Commitment Therapy for Psychosis. What's the evidence?

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Incorporation of alpha-amylase enzyme and a bioactive filler into hydrophilic, partially degradable, and bioactive cements (HDBCs) as a new approach to tailor simultaneously their degradation and bioactive behavior

Hydrophilic, partially degradable, and bioactive cements (HDBCs) are starch-containing cements intended to degrade partially in the human body and, in so doing, allow for bone ingrowth inside the pores formed during degradation. Therefore, the study of degradation and bioactivity behavior was performed to assess the suitability of the current HDBCs formulations to achieve those aims. The degradation profile of HDBCs was studied under different conditions, including incubation in phosphate-buffered saline (PBS) and PBS supplemented with R-amylase at different concentrations. Thermostable R-amylase was also added to some formulations to allow control of the degradation rate and its extent. In a second stage the simultaneous phenomena of enzymatic degradation and bioactivity (both in vitro) was studied. We observed that the degradation of starch present in HDBCs can be easily controlled by the amount of R-amylase added to the cement and high values of degradation may be achieved if high enough quantities of enzyme are incorporated. However, the maximum degradation extent is much more dependent on the total amount of starch present in the formulation than on the amount of enzyme added to it: for full pore connectivity, the amount of starch should be higher than the percolation threshold for a 3D specimen. Nonetheless, calcium phosphate was able to nucleate and spread in inner pores of the cement, formed due to degradation, if they were interconnected. For a more thorough covering of the pores with calcium phosphates the amount of starch present in HDBCs should be increased to be higher than the percolation threshold

In vitro assessment of the enzymatic degradation of several starch based biomaterials

The susceptibility of starch-based biomaterials to enzymatic degradation by amylolytic enzymes (glucoamylase and α-amylase) was investigated by means of incubating the materials with a buffer solution, containing enzymes at different concentrations and combinations, at 37 °C for 6 weeks. Two polymeric blends of corn starch with poly(ethylene-vinyl alcohol) copolymer and poly(Є-caprolactone), designated by SEVA-C and SPCL, respectively, were studied. The material degradation was characterized by gravimetry measurements, tensile mechanical testing, scanning electron microscopy (SEM), and Fourrier transform infrared-attenuated total reflectance (FTIR-ATR). The degradation liquors were analyzed for determination of reducing sugars, as a result of enzyme activity, and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used to identify the degradation products. All of the analysis performed showed that starch polymeric blends are susceptible to enzymatic degradation, as detected by increased weight loss and reducing sugars in solution. α-Amylase caused significant changes on the overall mechanical properties of the materials, with a decrease of about 65% and 58% being observed in the moduli for SEVA-C and SPCL, respectively, when compared with the control (samples incubated in buffer only). SEM analysis detected the presence of fractures and pores at the material’s surface as a result of starch degradation by amylolytic enzymes. FTIR spectra confirmed a decrease on the band corresponding to glycosidic linkage (-C-O-C-) of starch after incubation of the materials with α-amylase. In contrast, the incubation of the polymers in buffer only, did not cause significant changes on the material’s properties and morphology. Comparing the two materials, SEVA-C exhibited a higher degradability, which is related to the physicochemical structure of the materials and also to the fact that the starch concentration is higher in SEVA-C. The identification of the degradation products by HPAEC-PAD revealed that glucose was the major product of the enzymatic degradation of starch-based polymers. α-Amylase, as expected, is the key enzyme involved in the starch degradation, contributing to major changes on the physicochemical properties of the materials. Nevertheless, it was also found that starch-based polymers can also be degraded by other amylolytic enzymes but in a smaller extent.Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BPD/5744/2001 - Programa Operacional "Ciência, Tecnologia, Inovação (POCTI ) - União Europeia. Fundo Europeu de Desenvolvimento Regional (FEDER)

Biodiversity and characterization of marine mycota from Portuguese waters

Biodiversity and characterization of marine mycota from Portuguese waters.— The occurrence, diversity and similarity of marine fungi detected by the sum of direct and indirect observations in Fagus sylvatica and Pinus pinaster baits submerged at two Portuguese marinas are analyzed and discussed. In comparison with the data already published in 2010, the higher number of specimens considered in this study led to the higher number of very frequent taxa for these environments and substrata; the significant difference in substrata and also in fungal diversity detected at the two environments is also highlighted, in addition to the decrease in fungal similarity. Because the identification of Lulworthia spp., Fusarium sp., Graphium sp., Phoma sp. and Stachybotrys sp. down to species level was not possible, based only on the morphological characterization, a molecular approach based on the amplification of the LSU rDNA region was performed with isolates of these fungi. This was achieved for three isolates, identified as Fusarium solani, Graphium eumorphum and Stachybotrys chartarum. To achieve this with the other isolates which are more complex taxa, the sequencing of more regions will be considered.Biodiversidad y caracterización de los hongos marinos de las aguas portuguesas.— Se analiza y discute la presencia, la diversidad y la similitud de los hongos marinos detectados mediante la suma de observaciones directas e indirectas utilizando cebos de Fagus sylvatica y Pinus pinaster sumergidos en dos puertos deportivos portugueses. En comparación con los datos ya publicados en 2010, el mayor número de especímenes aquí considerados condujo a un mayor número de taxones muy frecuentes en estos sustratos y medios ambientales; también debe destacarse la diferencia significativa en los sustratos y también en la diversidad fúngica en los dos medios ambientales, además de la disminución de la similitud fúngica. Dado que no fue posible la identificación de Lulworthia spp., Fusarium sp., Graphium sp., Phoma sp., y Stachybotrys sp. hasta el nivel de especie, basándose únicamente en la caracterización morfológica, se llevó a cabo un estudio molecular basado en la amplificación de la región LSU ADNr con extractos de dichos hongos. Ello se consiguió en tres extractos puros, identificados como de Fusarium solani, Graphium eumorphum y Stachybotrys chartarum. Para llevar a cabo este proceso con otros extractos puros pertenecientes a taxones más complejos, se considerará la secuenciación de más regiones.publishe

A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis.

Biological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species.Edição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014